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P39954

- SAHH_YEAST

UniProt

P39954 - SAHH_YEAST

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Protein
Adenosylhomocysteinase
Gene
SAH1, YER043C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine.UniRule annotation

Cofactori

Binds 1 NAD per subunit.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei58 – 581Substrate By similarity
Binding sitei134 – 1341Substrate By similarity
Binding sitei159 – 1591Substrate By similarity
Binding sitei189 – 1891Substrate By similarity
Binding sitei193 – 1931Substrate By similarity
Binding sitei194 – 1941NAD By similarity
Binding sitei246 – 2461NAD By similarity
Binding sitei349 – 3491NAD By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi160 – 1623NAD By similarity
Nucleotide bindingi223 – 2286NAD By similarity
Nucleotide bindingi302 – 3043NAD By similarity

GO - Molecular functioni

  1. adenosylhomocysteinase activity Source: SGD
  2. protein binding Source: IntAct
Complete GO annotation...

GO - Biological processi

  1. S-adenosylhomocysteine catabolic process Source: SGD
  2. methionine metabolic process Source: SGD
  3. one-carbon metabolic process Source: UniProtKB-KW
  4. phosphatidylcholine biosynthetic process Source: SGD
  5. triglyceride metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciYEAST:YER043C-MONOMER.
ReactomeiREACT_188464. Sulfur amino acid metabolism.
UniPathwayiUPA00314; UER00076.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenosylhomocysteinase (EC:3.3.1.1)
Short name:
AdoHcyase
Alternative name(s):
S-adenosyl-L-homocysteine hydrolase
Gene namesi
Name:SAH1
Ordered Locus Names:YER043C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYER043c.
SGDiS000000845. SAH1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 449448AdenosylhomocysteinaseUniRule annotation
PRO_0000116937Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei393 – 3931Phosphothreonine4 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP39954.
PaxDbiP39954.
PeptideAtlasiP39954.
PRIDEiP39954.

Expressioni

Gene expression databases

GenevestigatoriP39954.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CAF16P435693EBI-16451,EBI-22808

Protein-protein interaction databases

BioGridi36779. 36 interactions.
DIPiDIP-5184N.
IntActiP39954. 6 interactions.
MINTiMINT-538156.
STRINGi4932.YER043C.

Structurei

3D structure databases

ProteinModelPortaliP39954.
SMRiP39954. Positions 8-449.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0499.
GeneTreeiENSGT00390000003626.
HOGENOMiHOG000227987.
KOiK01251.
OMAiTRVNIKP.
OrthoDBiEOG7FZ07M.

Family and domain databases

HAMAPiMF_00563. AdoHcyase.
InterProiIPR000043. Adenosylhomocysteinase.
IPR015878. Ado_hCys_hydrolase_NAD-bd.
IPR020082. S-Ado-L-homoCys_hydrolase_CS.
[Graphical view]
PANTHERiPTHR23420. PTHR23420. 1 hit.
PfamiPF05221. AdoHcyase. 1 hit.
PF00670. AdoHcyase_NAD. 1 hit.
[Graphical view]
PIRSFiPIRSF001109. Ad_hcy_hydrolase. 1 hit.
SMARTiSM00996. AdoHcyase. 1 hit.
SM00997. AdoHcyase_NAD. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00936. ahcY. 1 hit.
PROSITEiPS00738. ADOHCYASE_1. 1 hit.
PS00739. ADOHCYASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P39954-1 [UniParc]FASTAAdd to Basket

« Hide

MSAPAQNYKI ADISLAAFGR KEIELAEHEM PGLMAIRKAY GDVQPLKGAR    50
IAGCLHMTIQ TAVLIETLVA LGAEVTWSSC NIYSTQDHAA AAIAASGVPV 100
FAWKGETEEE YLWCIEQQLF AFKDNKKLNL ILDDGGDLTT LVHEKHPEML 150
EDCFGLSEET TTGVHHLYRM VKEGKLKVPA INVNDSVTKS KFDNLYGCRE 200
SLVDGIKRAT DVMLAGKVAV VAGYGDVGKG CAAALRGMGA RVLVTEIDPI 250
NALQAAMEGY QVVTMEDASH IGQVFVTTTG CRDIINGEHF INMPEDAIVC 300
NIGHFDIEID VAWLKANAKE CINIKPQVDR YLLSSGRHVI LLANGRLVNL 350
GCATGHSSFV MSCSFSNQVL AQIALFKSND KSFREKHIEF QKTGPFEVGV 400
HVLPKILDEA VAKFHLGNLG VRLTKLSKVQ SEYLGIPEEG PFKADHYRY 449
Length:449
Mass (Da):49,126
Last modified:February 1, 1995 - v1
Checksum:iD98D9DD329374F5B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18796 Genomic DNA. Translation: AAB64578.1.
AY692801 Genomic DNA. Translation: AAT92820.1.
BK006939 Genomic DNA. Translation: DAA07697.1.
PIRiS50546.
RefSeqiNP_010961.3. NM_001178934.3.

Genome annotation databases

EnsemblFungiiYER043C; YER043C; YER043C.
GeneIDi856766.
KEGGisce:YER043C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18796 Genomic DNA. Translation: AAB64578.1 .
AY692801 Genomic DNA. Translation: AAT92820.1 .
BK006939 Genomic DNA. Translation: DAA07697.1 .
PIRi S50546.
RefSeqi NP_010961.3. NM_001178934.3.

3D structure databases

ProteinModelPortali P39954.
SMRi P39954. Positions 8-449.
ModBasei Search...

Protein-protein interaction databases

BioGridi 36779. 36 interactions.
DIPi DIP-5184N.
IntActi P39954. 6 interactions.
MINTi MINT-538156.
STRINGi 4932.YER043C.

Proteomic databases

MaxQBi P39954.
PaxDbi P39954.
PeptideAtlasi P39954.
PRIDEi P39954.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YER043C ; YER043C ; YER043C .
GeneIDi 856766.
KEGGi sce:YER043C.

Organism-specific databases

CYGDi YER043c.
SGDi S000000845. SAH1.

Phylogenomic databases

eggNOGi COG0499.
GeneTreei ENSGT00390000003626.
HOGENOMi HOG000227987.
KOi K01251.
OMAi TRVNIKP.
OrthoDBi EOG7FZ07M.

Enzyme and pathway databases

UniPathwayi UPA00314 ; UER00076 .
BioCyci YEAST:YER043C-MONOMER.
Reactomei REACT_188464. Sulfur amino acid metabolism.

Miscellaneous databases

NextBioi 982947.
PROi P39954.

Gene expression databases

Genevestigatori P39954.

Family and domain databases

HAMAPi MF_00563. AdoHcyase.
InterProi IPR000043. Adenosylhomocysteinase.
IPR015878. Ado_hCys_hydrolase_NAD-bd.
IPR020082. S-Ado-L-homoCys_hydrolase_CS.
[Graphical view ]
PANTHERi PTHR23420. PTHR23420. 1 hit.
Pfami PF05221. AdoHcyase. 1 hit.
PF00670. AdoHcyase_NAD. 1 hit.
[Graphical view ]
PIRSFi PIRSF001109. Ad_hcy_hydrolase. 1 hit.
SMARTi SM00996. AdoHcyase. 1 hit.
SM00997. AdoHcyase_NAD. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00936. ahcY. 1 hit.
PROSITEi PS00738. ADOHCYASE_1. 1 hit.
PS00739. ADOHCYASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-393, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  5. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-393, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-393, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-393, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  9. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSAHH_YEAST
AccessioniPrimary (citable) accession number: P39954
Secondary accession number(s): D3DLU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 3, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

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