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Protein

Adenosylhomocysteinase

Gene

SAH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine.By similarity

Catalytic activityi

S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine.

Cofactori

NAD+Note: Binds 1 NAD+ per subunit.

Pathwayi: L-homocysteine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-homocysteine from S-adenosyl-L-homocysteine.
Proteins known to be involved in this subpathway in this organism are:
  1. Adenosylhomocysteinase (SAH1)
This subpathway is part of the pathway L-homocysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-homocysteine from S-adenosyl-L-homocysteine, the pathway L-homocysteine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei58SubstrateBy similarity1
Binding sitei134SubstrateBy similarity1
Binding sitei159SubstrateBy similarity1
Binding sitei189SubstrateBy similarity1
Binding sitei193SubstrateBy similarity1
Binding sitei194NADBy similarity1
Binding sitei246NADBy similarity1
Binding sitei349NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi160 – 162NADBy similarity3
Nucleotide bindingi223 – 228NADBy similarity6
Nucleotide bindingi302 – 304NADBy similarity3

GO - Molecular functioni

  • adenosylhomocysteinase activity Source: SGD
  • NAD binding Source: InterPro

GO - Biological processi

  • methionine metabolic process Source: SGD
  • one-carbon metabolic process Source: UniProtKB-KW
  • phosphatidylcholine biosynthetic process Source: SGD
  • S-adenosylhomocysteine catabolic process Source: SGD
  • S-adenosylmethionine cycle Source: GO_Central
  • triglyceride metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciYEAST:YER043C-MONOMER.
ReactomeiR-SCE-156581. Methylation.
R-SCE-1614635. Sulfur amino acid metabolism.
R-SCE-425381. Bicarbonate transporters.
UniPathwayiUPA00314; UER00076.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenosylhomocysteinase (EC:3.3.1.1)
Short name:
AdoHcyase
Alternative name(s):
S-adenosyl-L-homocysteine hydrolase
Gene namesi
Name:SAH1
Ordered Locus Names:YER043C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER043C.
SGDiS000000845. SAH1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001169372 – 449AdenosylhomocysteinaseAdd BLAST448

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Cross-linki21Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei393PhosphothreonineCombined sources1
Cross-linki413Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP39954.
PRIDEiP39954.

PTM databases

iPTMnetiP39954.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CAF16P435693EBI-16451,EBI-22808

Protein-protein interaction databases

BioGridi36779. 37 interactors.
DIPiDIP-5184N.
IntActiP39954. 6 interactors.
MINTiMINT-538156.

Structurei

3D structure databases

ProteinModelPortaliP39954.
SMRiP39954.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the adenosylhomocysteinase family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000003626.
HOGENOMiHOG000227987.
InParanoidiP39954.
KOiK01251.
OMAiEEYQWCL.
OrthoDBiEOG092C2224.

Family and domain databases

CDDicd00401. SAHH. 1 hit.
HAMAPiMF_00563. AdoHcyase. 1 hit.
InterProiIPR000043. Adenosylhomocysteinase.
IPR015878. Ado_hCys_hydrolase_NAD-bd.
IPR016040. NAD(P)-bd_dom.
IPR020082. S-Ado-L-homoCys_hydrolase_CS.
[Graphical view]
PANTHERiPTHR23420. PTHR23420. 1 hit.
PfamiPF05221. AdoHcyase. 2 hits.
PF00670. AdoHcyase_NAD. 1 hit.
[Graphical view]
PIRSFiPIRSF001109. Ad_hcy_hydrolase. 1 hit.
SMARTiSM00996. AdoHcyase. 1 hit.
SM00997. AdoHcyase_NAD. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00936. ahcY. 1 hit.
PROSITEiPS00738. ADOHCYASE_1. 1 hit.
PS00739. ADOHCYASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P39954-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAPAQNYKI ADISLAAFGR KEIELAEHEM PGLMAIRKAY GDVQPLKGAR
60 70 80 90 100
IAGCLHMTIQ TAVLIETLVA LGAEVTWSSC NIYSTQDHAA AAIAASGVPV
110 120 130 140 150
FAWKGETEEE YLWCIEQQLF AFKDNKKLNL ILDDGGDLTT LVHEKHPEML
160 170 180 190 200
EDCFGLSEET TTGVHHLYRM VKEGKLKVPA INVNDSVTKS KFDNLYGCRE
210 220 230 240 250
SLVDGIKRAT DVMLAGKVAV VAGYGDVGKG CAAALRGMGA RVLVTEIDPI
260 270 280 290 300
NALQAAMEGY QVVTMEDASH IGQVFVTTTG CRDIINGEHF INMPEDAIVC
310 320 330 340 350
NIGHFDIEID VAWLKANAKE CINIKPQVDR YLLSSGRHVI LLANGRLVNL
360 370 380 390 400
GCATGHSSFV MSCSFSNQVL AQIALFKSND KSFREKHIEF QKTGPFEVGV
410 420 430 440
HVLPKILDEA VAKFHLGNLG VRLTKLSKVQ SEYLGIPEEG PFKADHYRY
Length:449
Mass (Da):49,126
Last modified:February 1, 1995 - v1
Checksum:iD98D9DD329374F5B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18796 Genomic DNA. Translation: AAB64578.1.
AY692801 Genomic DNA. Translation: AAT92820.1.
BK006939 Genomic DNA. Translation: DAA07697.1.
PIRiS50546.
RefSeqiNP_010961.3. NM_001178934.3.

Genome annotation databases

EnsemblFungiiYER043C; YER043C; YER043C.
GeneIDi856766.
KEGGisce:YER043C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18796 Genomic DNA. Translation: AAB64578.1.
AY692801 Genomic DNA. Translation: AAT92820.1.
BK006939 Genomic DNA. Translation: DAA07697.1.
PIRiS50546.
RefSeqiNP_010961.3. NM_001178934.3.

3D structure databases

ProteinModelPortaliP39954.
SMRiP39954.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36779. 37 interactors.
DIPiDIP-5184N.
IntActiP39954. 6 interactors.
MINTiMINT-538156.

PTM databases

iPTMnetiP39954.

Proteomic databases

MaxQBiP39954.
PRIDEiP39954.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER043C; YER043C; YER043C.
GeneIDi856766.
KEGGisce:YER043C.

Organism-specific databases

EuPathDBiFungiDB:YER043C.
SGDiS000000845. SAH1.

Phylogenomic databases

GeneTreeiENSGT00390000003626.
HOGENOMiHOG000227987.
InParanoidiP39954.
KOiK01251.
OMAiEEYQWCL.
OrthoDBiEOG092C2224.

Enzyme and pathway databases

UniPathwayiUPA00314; UER00076.
BioCyciYEAST:YER043C-MONOMER.
ReactomeiR-SCE-156581. Methylation.
R-SCE-1614635. Sulfur amino acid metabolism.
R-SCE-425381. Bicarbonate transporters.

Miscellaneous databases

PROiP39954.

Family and domain databases

CDDicd00401. SAHH. 1 hit.
HAMAPiMF_00563. AdoHcyase. 1 hit.
InterProiIPR000043. Adenosylhomocysteinase.
IPR015878. Ado_hCys_hydrolase_NAD-bd.
IPR016040. NAD(P)-bd_dom.
IPR020082. S-Ado-L-homoCys_hydrolase_CS.
[Graphical view]
PANTHERiPTHR23420. PTHR23420. 1 hit.
PfamiPF05221. AdoHcyase. 2 hits.
PF00670. AdoHcyase_NAD. 1 hit.
[Graphical view]
PIRSFiPIRSF001109. Ad_hcy_hydrolase. 1 hit.
SMARTiSM00996. AdoHcyase. 1 hit.
SM00997. AdoHcyase_NAD. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00936. ahcY. 1 hit.
PROSITEiPS00738. ADOHCYASE_1. 1 hit.
PS00739. ADOHCYASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSAHH_YEAST
AccessioniPrimary (citable) accession number: P39954
Secondary accession number(s): D3DLU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 30, 2016
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.