ID   YEA6_YEAST              Reviewed;         335 AA.
AC   P39953; D3DLP1;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   27-MAR-2024, entry version 171.
DE   RecName: Full=Mitochondrial nicotinamide adenine dinucleotide transporter 2 {ECO:0000305};
DE   AltName: Full=Mitochondrial NAD(+) transporter 2 {ECO:0000303|PubMed:16291748};
GN   Name=YEA6; Synonyms=NDT2 {ECO:0000303|PubMed:16291748};
GN   OrderedLocusNames=YEL006W;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169868;
RA   Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E.,
RA   Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E.,
RA   Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S.,
RA   Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D.,
RA   Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X.,
RA   Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y.,
RA   Botstein D., Davis R.W.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
RL   Nature 387:78-81(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   IDENTIFICATION.
RX   PubMed=9178508;
RX   DOI=10.1002/(sici)1097-0061(199705)13:6<573::aid-yea107>3.0.co;2-i;
RA   el Moualij B., Duyckaerts C., Lamotte-Brasseur J., Sluse F.E.;
RT   "Phylogenetic classification of the mitochondrial carrier family of
RT   Saccharomyces cerevisiae.";
RL   Yeast 13:573-581(1997).
RN   [4]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [5]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=16291748; DOI=10.1074/jbc.m510425200;
RA   Todisco S., Agrimi G., Castegna A., Palmieri F.;
RT   "Identification of the mitochondrial NAD+ transporter in Saccharomyces
RT   cerevisiae.";
RL   J. Biol. Chem. 281:1524-1531(2006).
RN   [6]
RP   FUNCTION.
RX   PubMed=32906142; DOI=10.1038/s41586-020-2741-7;
RA   Luongo T.S., Eller J.M., Lu M.J., Niere M., Raith F., Perry C.,
RA   Bornstein M.R., Oliphint P., Wang L., McReynolds M.R., Migaud M.E.,
RA   Rabinowitz J.D., Johnson F.B., Johnsson K., Ziegler M., Cambronne X.A.,
RA   Baur J.A.;
RT   "SLC25A51 is a mammalian mitochondrial NAD+ transporter.";
RL   Nature 588:174-179(2020).
CC   -!- FUNCTION: Mitochondrial inner membrane carrier protein that mediates
CC       the import of NAD(+) into mitochondria (PubMed:16291748,
CC       PubMed:32906142). Can transport NAD(+) by unidirectional transport or
CC       by exchange with intramitochondrially generated dAMP and dGMP
CC       (PubMed:16291748). Also able to transport NAD(+) by exchange with AMP,
CC       GMP or deamido-NAD (+) in vitro (PubMed:16291748).
CC       {ECO:0000269|PubMed:16291748, ECO:0000269|PubMed:32906142}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=dAMP(in) + NAD(+)(out) = dAMP(out) + NAD(+)(in);
CC         Xref=Rhea:RHEA:65412, ChEBI:CHEBI:57540, ChEBI:CHEBI:58245;
CC         Evidence={ECO:0000269|PubMed:16291748};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:65413;
CC         Evidence={ECO:0000305|PubMed:16291748};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=dGMP(in) + NAD(+)(out) = dGMP(out) + NAD(+)(in);
CC         Xref=Rhea:RHEA:65416, ChEBI:CHEBI:57540, ChEBI:CHEBI:57673;
CC         Evidence={ECO:0000269|PubMed:16291748};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:65417;
CC         Evidence={ECO:0000305|PubMed:16291748};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=GMP(in) + NAD(+)(out) = GMP(out) + NAD(+)(in);
CC         Xref=Rhea:RHEA:65420, ChEBI:CHEBI:57540, ChEBI:CHEBI:58115;
CC         Evidence={ECO:0000269|PubMed:16291748};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:65421;
CC         Evidence={ECO:0000305|PubMed:16291748};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=AMP(in) + NAD(+)(out) = AMP(out) + NAD(+)(in);
CC         Xref=Rhea:RHEA:65424, ChEBI:CHEBI:57540, ChEBI:CHEBI:456215;
CC         Evidence={ECO:0000269|PubMed:16291748};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:65425;
CC         Evidence={ECO:0000305|PubMed:16291748};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=deamido-NAD(+)(in) + NAD(+)(out) = deamido-NAD(+)(out) +
CC         NAD(+)(in); Xref=Rhea:RHEA:65428, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:58437; Evidence={ECO:0000269|PubMed:16291748};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:65429;
CC         Evidence={ECO:0000305|PubMed:16291748};
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000305};
CC       Multi-pass membrane protein {ECO:0000305}.
CC   -!- MISCELLANEOUS: Present with 1630 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC       {ECO:0000305}.
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DR   EMBL; U18530; AAB64483.1; -; Genomic_DNA.
DR   EMBL; BK006939; DAA07645.1; -; Genomic_DNA.
DR   PIR; S50453; S50453.
DR   RefSeq; NP_010910.1; NM_001178821.1.
DR   AlphaFoldDB; P39953; -.
DR   SMR; P39953; -.
DR   BioGRID; 36725; 32.
DR   DIP; DIP-4659N; -.
DR   STRING; 4932.YEL006W; -.
DR   TCDB; 2.A.29.10.8; the mitochondrial carrier (mc) family.
DR   iPTMnet; P39953; -.
DR   MaxQB; P39953; -.
DR   PaxDb; 4932-YEL006W; -.
DR   PeptideAtlas; P39953; -.
DR   EnsemblFungi; YEL006W_mRNA; YEL006W; YEL006W.
DR   GeneID; 856712; -.
DR   KEGG; sce:YEL006W; -.
DR   AGR; SGD:S000000732; -.
DR   SGD; S000000732; YEA6.
DR   VEuPathDB; FungiDB:YEL006W; -.
DR   eggNOG; KOG0764; Eukaryota.
DR   GeneTree; ENSGT00940000176809; -.
DR   HOGENOM; CLU_015166_6_1_1; -.
DR   InParanoid; P39953; -.
DR   OMA; TLFRGCP; -.
DR   OrthoDB; 4772at2759; -.
DR   BioCyc; YEAST:G3O-30135-MONOMER; -.
DR   BioGRID-ORCS; 856712; 0 hits in 10 CRISPR screens.
DR   PRO; PR:P39953; -.
DR   Proteomes; UP000002311; Chromosome V.
DR   RNAct; P39953; Protein.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR   GO; GO:0051724; F:NAD transmembrane transporter activity; IDA:UniProtKB.
DR   GO; GO:1990549; P:mitochondrial NAD transmembrane transport; IDA:UniProtKB.
DR   GO; GO:0035352; P:NAD transmembrane transport; IMP:SGD.
DR   Gene3D; 1.50.40.10; Mitochondrial carrier domain; 2.
DR   InterPro; IPR002067; Mit_carrier.
DR   InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR   InterPro; IPR023395; Mt_carrier_dom_sf.
DR   InterPro; IPR044712; SLC25A32-like.
DR   PANTHER; PTHR45683:SF2; MITOCHONDRIAL NICOTINAMIDE ADENINE DINUCLEOTIDE TRANSPORTER 1-RELATED; 1.
DR   PANTHER; PTHR45683; MITOCHONDRIAL NICOTINAMIDE ADENINE DINUCLEOTIDE TRANSPORTER 1-RELATED-RELATED; 1.
DR   Pfam; PF00153; Mito_carr; 3.
DR   PRINTS; PR00926; MITOCARRIER.
DR   SUPFAM; SSF103506; Mitochondrial carrier; 1.
DR   PROSITE; PS50920; SOLCAR; 3.
PE   1: Evidence at protein level;
KW   Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW   Repeat; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..335
FT                   /note="Mitochondrial nicotinamide adenine dinucleotide
FT                   transporter 2"
FT                   /id="PRO_0000090694"
FT   TRANSMEM        42..62
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        104..124
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        142..162
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        198..218
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        247..267
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        298..318
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   REPEAT          36..128
FT                   /note="Solcar 1"
FT                   /evidence="ECO:0000255"
FT   REPEAT          136..225
FT                   /note="Solcar 2"
FT                   /evidence="ECO:0000255"
FT   REPEAT          241..329
FT                   /note="Solcar 3"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   335 AA;  36961 MW;  91555A2CE81286AC CRC64;
     MNNGDNKTTL ENSKNASLAN GNYAIPTKLN RLKKNADPRV AAISGALSGA LSAMLVCPFD
     VAKTRLQAQG LQNMTHQSQH YKGFFGTFAT IFKDEGAAGL YKGLQPTVLG YIPTLMIYFS
     VYDFCRKYSV DIFPHSPFLS NASSAITAGA ISTVATNPIW VVKTRLMLQT GIGKYSTHYK
     GTIDTFRKII QQEGAKALYA GLVPALLGML NVAIQFPLYE NLKIRFGYSE STDVSTDVTS
     SNFQKLILAS MLSKMVASTV TYPHEILRTR MQLKSDLPNT VQRHLLPLIK ITYRQEGFAG
     FYSGFATNLV RTVPAAVVTL VSFEYSKKYL TTFFQ
//