P39953 (YEA6_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial nicotinamide adenine dinucleotide transporter 2

Alternative name(s):
Mitochondrial NAD(+) transporter 2

Gene names

Name:	YEA6
Synonyms:	NDT2
Ordered Locus Names:	YEL006W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mitochondrial inner membrane carrier protein involved in the import of NAD+ into mitochondria by unidirectional transport or by exchange with intramitochondrially generated dAMP and dGMP. Ref.3 Ref.5
Subcellular location	Mitochondrion inner membrane; Multi-pass membrane protein Potential.
Miscellaneous	Present with 1630 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the mitochondrial carrier (TC 2.A.29) family. [View classification] Contains 3 Solcar repeats.

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Repeat Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	NAD transmembrane transport Inferred from mutant phenotype Ref.5. Source: SGD
Cellular_component	integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD mitochondrial inner membrane Inferred from sequence or structural similarity PubMed 10930523. Source: SGD
Molecular_function	NAD transporter activity Inferred from mutant phenotype Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	Mitochondrial nicotinamide adenine dinucleotide transporter 2		PRO_0000090694
Regions
	Transmembrane	42 – 62	21	Helical; Name=1; Potential
	Transmembrane	104 – 124	21	Helical; Name=2; Potential
	Transmembrane	142 – 162	21	Helical; Name=3; Potential
	Transmembrane	198 – 218	21	Helical; Name=4; Potential
	Transmembrane	247 – 267	21	Helical; Name=5; Potential
	Transmembrane	298 – 318	21	Helical; Name=6; Potential
	Repeat	36 – 128	93	Solcar 1
	Repeat	136 – 225	90	Solcar 2
	Repeat	241 – 329	89	Solcar 3
Amino acid modifications
	Modified residue	275	1	Phosphoserine Ref.6
	Modified residue	280	1	Phosphothreonine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P39953 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 91555A2CE81286AC	FASTA	335	36,961
	10 20 30 40 50 60 MNNGDNKTTL ENSKNASLAN GNYAIPTKLN RLKKNADPRV AAISGALSGA LSAMLVCPFD 70 80 90 100 110 120 VAKTRLQAQG LQNMTHQSQH YKGFFGTFAT IFKDEGAAGL YKGLQPTVLG YIPTLMIYFS 130 140 150 160 170 180 VYDFCRKYSV DIFPHSPFLS NASSAITAGA ISTVATNPIW VVKTRLMLQT GIGKYSTHYK 190 200 210 220 230 240 GTIDTFRKII QQEGAKALYA GLVPALLGML NVAIQFPLYE NLKIRFGYSE STDVSTDVTS 250 260 270 280 290 300 SNFQKLILAS MLSKMVASTV TYPHEILRTR MQLKSDLPNT VQRHLLPLIK ITYRQEGFAG 310 320 330 FYSGFATNLV RTVPAAVVTL VSFEYSKKYL TTFFQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Phylogenetic classification of the mitochondrial carrier family of Saccharomyces cerevisiae." el Moualij B., Duyckaerts C., Lamotte-Brasseur J., Sluse F.E. Yeast 13:573-581(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Identification of the mitochondrial NAD+ transporter in Saccharomyces cerevisiae." Todisco S., Agrimi G., Castegna A., Palmieri F. J. Biol. Chem. 281:1524-1531(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-275 AND THR-280, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18530 Genomic DNA. Translation: AAB64483.1. BK006939 Genomic DNA. Translation: DAA07645.1.
PIR	S50453.
RefSeq	NP_010910.1. NM_001178821.1.
3D structure databases
ProteinModelPortal	P39953.
SMR	P39953. Positions 40-335.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4659N.
MINT	MINT-502454.
STRING	4932.YEL006W.
Proteomic databases
PaxDb	P39953.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YEL006W; YEL006W; YEL006W.
GeneID	856712.
KEGG	sce:YEL006W.
Organism-specific databases
CYGD	YEL006w.
SGD	S000000732. YEA6.
Phylogenomic databases
eggNOG	NOG314559.
GeneTree	ENSGT00600000084419.
HOGENOM	HOG000188210.
KO	K15115.
OMA	CIRKVYH.
OrthoDB	EOG4CG3JQ.
Gene expression databases
Genevestigator	P39953.
GermOnline	YEL006W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.50.40.10. 1 hit.
InterPro	IPR002067. Mit_carrier. IPR018108. Mitochondrial_sb/sol_carrier. IPR023395. Mt_carrier_dom. [Graphical view]
Pfam	PF00153. Mito_carr. 3 hits. [Graphical view]
PRINTS	PR00926. MITOCARRIER.
SUPFAM	SSF103506. Mitoch_carrier. 1 hit.
PROSITE	PS50920. SOLCAR. 3 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	982787.

Entry information

Entry name

YEA6_YEAST

Accession

Primary (citable) accession number: P39953
Secondary accession number(s): D3DLP1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program