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Protein

Mitochondrial inner membrane protein OXA1

Gene

OXA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mitochondrial inner membrane insertase that mediates the insertion of both mitochondrion-encoded precursors and nuclear-encoded proteins from the matrix into the inner membrane. Links mitoribosomes with the inner membrane (PubMed:22904327). Forms pores capable of accommodating translocating protein segments (PubMed:22829595). Essential for the activity and assembly of cytochrome c oxidase (PubMed:22904327). Plays a central role in the translocation and export of the N-terminal part of the COX2 protein into the mitochondrial intermembrane space.5 Publications

GO - Molecular functioni

  • ion channel activity Source: SGD
  • membrane insertase activity Source: SGD
  • mitochondrial ribosome binding Source: SGD

GO - Biological processi

  • ion transmembrane transport Source: GOC
  • mitochondrial genome maintenance Source: SGD
  • mitochondrial proton-transporting ATP synthase complex assembly Source: SGD
  • protein import into mitochondrial inner membrane Source: SGD
  • protein insertion into mitochondrial membrane from inner side Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-30315-MONOMER.

Protein family/group databases

TCDBi2.A.9.1.1. the membrane protein insertase (yidc/alb3/oxa1) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial inner membrane protein OXA1Curated
Alternative name(s):
Cytochrome oxidase biogenesis protein OXA1Curated
Oxidase assembly protein 11 Publication
Gene namesi
Name:OXA11 Publication
Synonyms:PET14021 Publication
Ordered Locus Names:YER154WImported
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER154W.
SGDiS000000956. OXA1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini43 – 11876Mitochondrial intermembrane1 PublicationAdd
BLAST
Transmembranei119 – 13921HelicalSequence analysisAdd
BLAST
Topological domaini140 – 19960Mitochondrial matrixCuratedAdd
BLAST
Transmembranei200 – 22021HelicalSequence analysisAdd
BLAST
Topological domaini221 – 23919Mitochondrial intermembraneCuratedAdd
BLAST
Transmembranei240 – 26021HelicalSequence analysisAdd
BLAST
Topological domaini261 – 27515Mitochondrial matrixCuratedAdd
BLAST
Transmembranei276 – 29217HelicalSequence analysisAdd
BLAST
Topological domaini293 – 2975Mitochondrial intermembraneCurated
Transmembranei298 – 31619HelicalSequence analysisAdd
BLAST
Topological domaini317 – 40286Mitochondrial matrixCuratedAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • mitochondrial crista Source: SGD
  • mitochondrial inner boundary membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Disruption phenotypei

Leads to reduced steady-state levels and activities of the mitochondrial ATP/ADP carrier protein AAC2.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi128 – 1281W → R: Leads to respiratory deficiency. 1 Publication
Mutagenesisi129 – 1291W → R: Leads to a defect in complex V assembly and subsequent respiratory deficiency. 1 Publication
Mutagenesisi213 – 2131G → R: Leads to a defect in complex V assembly and subsequent respiratory deficiency. 1 Publication
Mutagenesisi229 – 2291F → S: Leads to a defect in complexes IV and V assembly, and subsequent respiratory deficiency. 1 Publication
Mutagenesisi240 – 2401L → S: Leads to a defect in complex IV assembly and subsequent respiratory deficiency. 1 Publication
Mutagenesisi249 – 2491L → S: Leads to respiratory deficiency. 1 Publication
Mutagenesisi250 – 2501Q → R: Leads to respiratory deficiency. 1 Publication
Mutagenesisi300 – 3001L → P: Leads to a defect in complex V assembly and subsequent respiratory deficiency. 1 Publication
Mutagenesisi301 – 3011Y → D: Leads to respiratory deficiency. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4242Mitochondrion1 PublicationAdd
BLAST
Chaini43 – 402360Mitochondrial inner membrane protein OXA1PRO_0000020362Add
BLAST

Proteomic databases

MaxQBiP39952.

Miscellaneous databases

PMAP-CutDBP39952.

Interactioni

Subunit structurei

Interacts with the large ribosome subunit of mitochondrial ribosome (PubMed:14657017, PubMed:22904327). Interacts directly with MRP20 (PubMed:14657017). Interacts with OXA1 (PubMed:25609543).3 Publications

Protein-protein interaction databases

BioGridi36904. 50 interactions.
DIPiDIP-3870N.
IntActiP39952. 13 interactions.
MINTiMINT-517014.

Structurei

3D structure databases

ProteinModelPortaliP39952.
SMRiP39952. Positions 131-316.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the OXA1/ALB3/YidC family.Curated

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00530000063506.
HOGENOMiHOG000248029.
InParanoidiP39952.
KOiK03217.
OMAiVTICARS.
OrthoDBiEOG7V4B7X.

Family and domain databases

InterProiIPR001708. Membrane_insert_OXA1/ALB3/YidC.
[Graphical view]
PANTHERiPTHR12428. PTHR12428. 1 hit.
PfamiPF02096. 60KD_IMP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P39952-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKLTSRLVT SRFAASSRLA TARTIVLPRP HPSWISFQAK RFNSTGPNAN
60 70 80 90 100
DVSEIQTQLP SIDELTSSAP SLSASTSDLI ANTTQTVGEL SSHIGYLNSI
110 120 130 140 150
GLAQTWYWPS DIIQHVLEAV HVYSGLPWWG TIAATTILIR CLMFPLYVKS
160 170 180 190 200
SDTVARNSHI KPELDALNNK LMSTTDLQQG QLVAMQRKKL LSSHGIKNRW
210 220 230 240 250
LAAPMLQIPI ALGFFNALRH MANYPVDGFA NQGVAWFTDL TQADPYLGLQ
260 270 280 290 300
VITAAVFISF TRLGGETGAQ QFSSPMKRLF TILPIISIPA TMNLSSAVVL
310 320 330 340 350
YFAFNGAFSV LQTMILRNKW VRSKLKITEV AKPRTPIAGA SPTENMGIFQ
360 370 380 390 400
SLKHNIQKAR DQAERRQLMQ DNEKKLQESF KEKRQNSKIK IVHKSNFINN

KK
Length:402
Mass (Da):44,816
Last modified:February 1, 1995 - v1
Checksum:i108890B8F2EE67ED
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti108 – 1081W → R in CAA54675 (PubMed:8196054).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77558 Genomic DNA. Translation: CAA54675.1.
X74456 Genomic DNA. Translation: CAA52465.1.
U18917 Genomic DNA. Translation: AAB64681.1.
AY693132 Genomic DNA. Translation: AAT93151.1.
BK006939 Genomic DNA. Translation: DAA07815.1.
PIRiS47329.
RefSeqiNP_011081.1. NM_001179044.1.

Genome annotation databases

EnsemblFungiiYER154W; YER154W; YER154W.
GeneIDi856898.
KEGGisce:YER154W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77558 Genomic DNA. Translation: CAA54675.1.
X74456 Genomic DNA. Translation: CAA52465.1.
U18917 Genomic DNA. Translation: AAB64681.1.
AY693132 Genomic DNA. Translation: AAT93151.1.
BK006939 Genomic DNA. Translation: DAA07815.1.
PIRiS47329.
RefSeqiNP_011081.1. NM_001179044.1.

3D structure databases

ProteinModelPortaliP39952.
SMRiP39952. Positions 131-316.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36904. 50 interactions.
DIPiDIP-3870N.
IntActiP39952. 13 interactions.
MINTiMINT-517014.

Protein family/group databases

TCDBi2.A.9.1.1. the membrane protein insertase (yidc/alb3/oxa1) family.

Proteomic databases

MaxQBiP39952.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER154W; YER154W; YER154W.
GeneIDi856898.
KEGGisce:YER154W.

Organism-specific databases

EuPathDBiFungiDB:YER154W.
SGDiS000000956. OXA1.

Phylogenomic databases

GeneTreeiENSGT00530000063506.
HOGENOMiHOG000248029.
InParanoidiP39952.
KOiK03217.
OMAiVTICARS.
OrthoDBiEOG7V4B7X.

Enzyme and pathway databases

BioCyciYEAST:G3O-30315-MONOMER.

Miscellaneous databases

PMAP-CutDBP39952.
PROiP39952.

Family and domain databases

InterProiIPR001708. Membrane_insert_OXA1/ALB3/YidC.
[Graphical view]
PANTHERiPTHR12428. PTHR12428. 1 hit.
PfamiPF02096. 60KD_IMP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "OXA1, a Saccharomyces cerevisiae nuclear gene whose sequence is conserved from prokaryotes to eukaryotes controls cytochrome oxidase biogenesis."
    Bonnefoy N., Chalvet F., Hamel P., Slonimski P.P., Dujardin G.
    J. Mol. Biol. 239:201-212(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 28383 / FL100 / VTT C-80102.
  2. "PET1402, a nuclear gene required for proteolytic processing of cytochrome oxidase subunit 2 in yeast."
    Bauer M., Behrens M., Esser K., Michaelis G., Pratje E.
    Mol. Gen. Genet. 245:272-278(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "Insertion into the mitochondrial inner membrane of a polytopic protein, the nuclear-encoded Oxa1p."
    Herrmann J.M., Neupert W., Stuart R.A.
    EMBO J. 16:2217-2226(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TRANSIT PEPTIDE, TOPOLOGY.
  7. "Oxa1p, an essential component of the N-tail protein export machinery in mitochondria."
    Hell K., Herrmann J.M., Pratje E., Neupert W., Stuart R.A.
    Proc. Natl. Acad. Sci. U.S.A. 95:2250-2255(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Yeast Oxa1 interacts with mitochondrial ribosomes: the importance of the C-terminal region of Oxa1."
    Jia L., Dienhart M., Schramp M., McCauley M., Hell K., Stuart R.A.
    EMBO J. 22:6438-6447(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MRP20.
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "Insertion of proteins into the inner membrane of mitochondria: the role of the Oxa1 complex."
    Stuart R.A.
    Biochim. Biophys. Acta 1592:79-87(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  11. "A mutational analysis reveals new functional interactions between domains of the Oxa1 protein in Saccharomyces cerevisiae."
    Mathieu L., Bourens M., Marsy S., Hlavacek O., Panozzo C., Dujardin G.
    Mol. Microbiol. 75:474-488(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF TRP-128; TRP-129; GLY-213; PHE-229; LEU-240; LEU-249; GLN-250; LEU-300 AND TYR-301.
  12. "The mitochondrial oxidase assembly protein1 (Oxa1) insertase forms a membrane pore in lipid bilayers."
    Kruger V., Deckers M., Hildenbeutel M., van der Laan M., Hellmers M., Dreker C., Preuss M., Herrmann J.M., Rehling P., Wagner R., Meinecke M.
    J. Biol. Chem. 287:33314-33326(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  13. "Oxa1-ribosome complexes coordinate the assembly of cytochrome C oxidase in mitochondria."
    Keil M., Bareth B., Woellhaf M.W., Peleh V., Prestele M., Rehling P., Herrmann J.M.
    J. Biol. Chem. 287:34484-34493(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BINDING TO MITORIBOSOMES.
  14. "The membrane insertase Oxa1 is required for efficient import of carrier proteins into mitochondria."
    Hildenbeutel M., Theis M., Geier M., Haferkamp I., Neuhaus H.E., Herrmann J.M., Ott M.
    J. Mol. Biol. 423:590-599(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION.
  15. "The inner-mitochondrial distribution of Oxa1 depends on the growth conditions and on the availability of substrates."
    Stoldt S., Wenzel D., Hildenbeutel M., Wurm C.A., Herrmann J.M., Jakobs S.
    Mol. Biol. Cell 23:2292-2301(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  16. "Organization of the mitochondrial translation machinery studied in situ by cryoelectron tomography."
    Pfeffer S., Woellhaf M.W., Herrmann J.M., Forster F.
    Nat. Commun. 6:6019-6019(2015) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MBA1.

Entry informationi

Entry nameiOXA1_YEAST
AccessioniPrimary (citable) accession number: P39952
Secondary accession number(s): D3DM61
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 8, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6550 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.