Reviewed, UniProtKB/Swiss-Prot P39951 (CDC2_RAT)

Names and origin

Protein names

Recommended name:
    Cell division control protein 2 homolog
    EC=2.7.11.22
    EC=2.7.11.23
Alternative name(s):
    p34 protein kinase
    Cyclin-dependent kinase 1
      Short name=CDK1

Gene names

Name:	Cdc2
Synonyms:	Cdc2a

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Enzyme regulation	Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-161 activates it By similarity.
Subunit structure	Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with DLGAP5. Interacts with catalytically active CCNB1 and RALBP1 during mitosis to form an endocytotic complex during interphase.
Subcellular location	Nucleus Ref.3.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Acetylation Isopeptide bond Phosphoprotein Ubl conjugation
Gene Ontology (GO)
Biological process	G1 phase of mitotic cell cycle Traceable author statement. Source: RGD cell aging Inferred from direct assay. Source: RGD cell division Inferred from electronic annotation. Source: UniProtKB-KW chromosome condensation Inferred from mutant phenotype. Source: RGD mitosis Inferred from electronic annotation. Source: UniProtKB-KW organ regeneration Inferred from mutant phenotype. Source: RGD positive regulation of cardiac muscle cell proliferation Inferred from mutant phenotype. Source: RGD positive regulation of mitotic cell cycle Inferred from mutant phenotype. Source: RGD response to cadmium ion Inferred from expression pattern. Source: RGD response to drug Inferred from expression pattern. Source: RGD response to ethanol Inferred from direct assay. Source: RGD response to hydrogen peroxide Inferred from expression pattern. Source: RGD response to organic nitrogen Inferred from direct assay. Source: RGD
Cellular component	nucleus Inferred from direct assay. Source: RGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin binding Inferred from physical interaction. Source: RGD cyclin-dependent protein kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 297	297	Cell division control protein 2 homolog		PRO_0000085727
Regions
	Domain	4 – 287	284	Protein kinase
	Nucleotide binding	10 – 18	9	ATP By similarity
Sites
	Active site	128	1	Proton acceptor By similarity
	Binding site	33	1	ATP By similarity
Amino acid modifications
	Modified residue	4	1	Phosphotyrosine By similarity
	Modified residue	6	1	N6-acetyllysine By similarity
	Modified residue	14	1	Phosphothreonine By similarity
	Modified residue	15	1	Phosphotyrosine By similarity
	Modified residue	19	1	Phosphotyrosine By similarity
	Modified residue	39	1	Phosphoserine By similarity
	Modified residue	46	1	Phosphoserine By similarity
	Modified residue	77	1	Phosphotyrosine By similarity
	Modified residue	141	1	Phosphothreonine By similarity
	Modified residue	160	1	Phosphotyrosine By similarity
	Modified residue	161	1	Phosphothreonine By similarity
	Modified residue	178	1	Phosphoserine By similarity
	Modified residue	181	1	Phosphotyrosine By similarity
	Modified residue	222	1	Phosphothreonine By similarity
	Modified residue	233	1	Phosphoserine By similarity
	Modified residue	248	1	Phosphoserine By similarity
	Cross-link	89		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P39951-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 73695017E127178A	FASTA	297	34,135
	10 20 30 40 50 60 MEDYIKIEKI GEGTYGVVYK GRHRTTGQIV AMKKIRLESE EEGVPSTAIR EISLLKELRH 70 80 90 100 110 120 PNIVSLQDVL MQDSRLYLIF EFLSMDLKKY LDSIPPGQFM DSSLVKSYLY QILQGIVFCH 130 140 150 160 170 180 SRRVLHRDLK PQNLLIDDKG TIKLADFGLA RAFGIPIRVY THEVVTLWYR SPEVLLGSAR 190 200 210 220 230 240 YSTPVDIWSI GTIFAELATK KPLFHGDSEI DQLFRIFRAL GTPNNEVWPE VESLQDYKNT 250 260 270 280 290 FPKWKPGSLA SHVKNLDENG LDLLSKMLVY DPAKRISGKM ALKHPYFDDL DNQIKKM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Kanaoka Y., Nojima H., Okayama H. Submitted (JUL-1991) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Thymus.
[3]	"The cdc2 kinase is a nuclear protein that is essential for mitosis in mammalian cells." Riabowol K., Draetta G., Brizuela L., Vandre D., Beach D. Cell 57:393-401(1989) [PubMed: 2541912] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X60767 mRNA. Translation: CAA43177.1. BC091549 mRNA. Translation: AAH91549.1.
IPI	IPI00190390.
PIR	S24913.
RefSeq	NP_062169.1.
UniGene	Rn.6934
3D structure databases
SMR	P39951. Positions 1-294.
ModBase	Search...
Protein-protein interaction databases
IntAct	P39951. 3 interactions.
STRING	P39951.
PTM databases
PhosphoSite	P39951.
Genome annotation databases
Ensembl	ENSRNOT00000000783; ENSRNOP00000000783; ENSRNOG00000000632; Rattus norvegicus. [Genome view]
GeneID	54237.
KEGG	rno:54237.
UCSC	NM_019296. rat.
Organism-specific databases
CTD	54237.
RGD	2319. Cdc2a.
Phylogenomic databases
eggNOG	roNOG07497.
HOVERGEN	P39951.
InParanoid	P39951.
OMA	MLIYDPA.
OrthoDB	EOG95QKZV.
PhylomeDB	P39951.
Enzyme and pathway databases
BRENDA	2.7.11.22. 248. 2.7.11.23. 248.
Gene expression databases
ArrayExpress	P39951.
Genevestigator	P39951.
GermOnline	ENSRNOG00000000632. Rattus norvegicus.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	610684.

Entry information

Entry name

CDC2_RAT

Accession

Primary (citable) accession number: P39951
Secondary accession number(s): Q5BJB4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	February 9, 2010
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)