P39949 (CCNE1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
G1/S-specific cyclin-E1

Gene names

Name:	Ccne1
Synonyms:	Ccne

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Essential for the control of the cell cycle at the G1/S (start) transition.
Subunit structure	Interacts with a member of the CDK2/CDK protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Found in a complex with CDK2, CABLES1 and CCNA1. Part of a complex consisting of UHRF2, CDK2 and CCNE1. Interacts directly with UHRF2; the interaction ubiquitinates CCNE1 and appears to occur independently of CCNE1 phosphorylation By similarity.
Subcellular location	Nucleus By similarity.
Post-translational modification	Phosphorylation of Thr-396 and Ser-400 accelerates degradation via the ubiquitin proteasome pathway. Ubiquitinated by UHRF2; appears to occur independently of phosphorylation By similarity.
Sequence similarities	Belongs to the cyclin family. Cyclin E subfamily.
Sequence caution	The sequence BAA03116.1 differs from that shown. Reason: Frameshift at position 15.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Cellular component	Nucleus
Molecular function	Cyclin
PTM	Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	antral ovarian follicle growth Inferred from expression pattern PubMed 17084963. Source: RGD cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular response to nutrient Inferred from expression pattern PubMed 17979132. Source: RGD liver development Inferred from expression pattern PubMed 15809057. Source: RGD organ regeneration Inferred from expression pattern PubMed 16273211. Source: RGD positive regulation of cell differentiation Inferred from direct assay PubMed 15642789. Source: RGD regulation of cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: InterPro response to corticosterone stimulus Inferred from expression pattern PubMed 12807724. Source: RGD response to cytokine stimulus Inferred from expression pattern PubMed 17483238. Source: RGD response to drug Inferred from expression pattern PubMed 16760380. Source: RGD response to estradiol stimulus Inferred from expression pattern PubMed 16019103. Source: RGD response to ethanol Inferred from expression pattern PubMed 16689928. Source: RGD response to methylmercury Inferred from expression pattern PubMed 17056119. Source: RGD response to progesterone stimulus Inferred from expression pattern PubMed 12810531. Source: RGD response to purine-containing compound Inferred from expression pattern PubMed 16759516. Source: RGD response to vitamin E Inferred from expression pattern PubMed 16298738. Source: RGD
Cellular_component	centrosome Inferred from direct assay PubMed 15514162. Source: RGD cytoplasm Inferred from direct assay PubMed 10952244. Source: RGD nucleus Inferred from direct assay PubMed 14614307. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 411	411	G1/S-specific cyclin-E1		PRO_0000080451
Amino acid modifications
	Modified residue	77	1	Phosphothreonine By similarity
	Modified residue	104	1	Phosphoserine By similarity
	Modified residue	388	1	Phosphoserine By similarity
	Modified residue	396	1	Phosphothreonine By similarity
	Modified residue	400	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	221	1	A → T in BAA09640. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P39949 [UniParc]. Last modified November 13, 2007. Version 2. Checksum: 24CA81652056C036	FASTA	411	47,482
	10 20 30 40 50 60 MPRERKERDS KDHTKMKEEG GSDLSVRSRK RKPNVPVFLQ DPDEEIAKID KTVKSQDSSQ 70 80 90 100 110 120 PWDDDSACVD PCSFIPTPNK EEDNELEYPK TAFQPRKIRP PRASPLPVLN WANREEVWRI 130 140 150 160 170 180 MLNKEKTYLR DEHFLQRHPL LQARMRAVLL DWLMEVCEVY KLHRETFYLA QDFFDRYMAS 190 200 210 220 230 240 QQNIIKTLLQ LIGISALFIA SKLEEIYPPK LHQFAYVTDG ACSGDEILTM ELMMMKALKW 250 260 270 280 290 300 RLSPLTIVSW LNVYVQVAYV NDTGEVLMPQ YPQQVFVQIA ELLDLCVLDV GCLEFPYGVL 310 320 330 340 350 360 AASALYHFSS LELMQKVSGY QWCDIEKCVK WMVPFAMVIR EMGSSKLKHF RGVPMEDSHN 370 380 390 400 410 IQTHTNSLDL LDKAQAKKAI LSEQNRISPP PSGVLTPPHS SKKQSSEQET E

References

[1]	"Cyclin G: a new mammalian cyclin with homology to fission yeast Cig1." Tamura K., Kanaoka Y., Jinno S., Nagata A., Ogiso Y., Shimizu K., Hayakawa T., Nojima H., Okayama H. Oncogene 8:2113-2118(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney.
[2]	"Synergistic gene expressions of cyclin E, cdk2, cdk5 and E2F-1 during the prolactin-induced G1/S transition in rat Nb2 pre-T lymphoma cells." Hosokawa Y., Yang M., Kaneko S., Tanaka M., Nakashima K. Biochem. Mol. Biol. Int. 37:393-399(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 152-222.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D14015 mRNA. Translation: BAA03116.1. Frameshift. D63164 mRNA. Translation: BAA09640.1.
IPI	IPI00190353.
UniGene	Rn.15455.
3D structure databases
ProteinModelPortal	P39949.
SMR	P39949. Positions 104-373.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-29865N.
Proteomic databases
PaxDb	P39949.
PRIDE	P39949.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
RGD	2294. Ccne1.
Phylogenomic databases
eggNOG	COG5024.
HOGENOM	HOG000231743.
HOVERGEN	HBG050834.
InParanoid	P39949.
OrthoDB	EOG45756T.
Gene expression databases
ArrayExpress	P39949.
Genevestigator	P39949.
GermOnline	ENSRNOG00000014786. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.472.10. 2 hits.
InterPro	IPR013763. Cyclin-like. IPR014400. Cyclin_A/B/D/E. IPR004367. Cyclin_C-dom. IPR006671. Cyclin_N. [Graphical view]
Pfam	PF02984. Cyclin_C. 1 hit. PF00134. Cyclin_N. 1 hit. [Graphical view]
PIRSF	PIRSF001771. Cyclin_A_B_D_E. 1 hit.
SMART	SM00385. CYCLIN. 1 hit. [Graphical view]
SUPFAM	SSF47954. Cyclin_like. 1 hit.
PROSITE	PS00292. CYCLINS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CCNE1_RAT

Accession

Primary (citable) accession number: P39949
Secondary accession number(s): O09138

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	November 13, 2007
Last modified:	May 1, 2013
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program