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Protein

Nuclear distribution protein PAC1

Gene

PAC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Positively regulates the activity of the minus-end directed microtubule motor protein dynein. Plays a central role in positioning the mitotic spindle at the bud neck during cell division. Targets cytoplasmic dynein to microtubule plus ends, thereby promoting dynein-mediated microtubule sliding along the bud cortex and consequently the movement of the mitotic spindle to the bud neck. Following plus end binding, dynein may be offloaded to the cortex by NUM1. Required for viability in the absence of the kinesin-related microtubule-dependent motor protein CIN8.UniRule annotation3 Publications

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • microtubule plus-end binding Source: SGD

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • establishment of mitotic spindle orientation Source: UniProtKB-HAMAP
  • mitotic nuclear division Source: UniProtKB-KW
  • nuclear migration along microtubule Source: SGD
  • positive regulation of ATP-dependent microtubule motor activity, plus-end-directed Source: SGD
  • positive regulation of microtubule plus-end binding Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33759-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear distribution protein PAC1UniRule annotation
Alternative name(s):
Lissencephaly-1 homologUniRule annotation
Short name:
LIS-1UniRule annotation
nudF homologUniRule annotation
Gene namesi
Name:PAC1UniRule annotation
Synonyms:LIS1UniRule annotation
Ordered Locus Names:YOR269W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR269W.
SGDiS000005795. PAC1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic microtubule Source: SGD
  • microtubule associated complex Source: UniProtKB-HAMAP
  • nucleus Source: SGD
  • spindle pole Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 494494Nuclear distribution protein PAC1PRO_0000051113Add
BLAST

Proteomic databases

MaxQBiP39946.

Interactioni

Subunit structurei

Self-associates. Interacts with NDL1. May form a complex with dynein.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-12838,EBI-12838
KEL1P388533EBI-12838,EBI-9619
NDL1Q065683EBI-12838,EBI-33247
NNF1P471493EBI-12838,EBI-12098

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • microtubule plus-end binding Source: SGD

Protein-protein interaction databases

BioGridi34658. 147 interactions.
DIPiDIP-1485N.
IntActiP39946. 9 interactions.
MINTiMINT-399947.

Structurei

3D structure databases

ProteinModelPortaliP39946.
SMRiP39946. Positions 141-493.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 4633LisHUniRule annotationAdd
BLAST
Repeati153 – 19240WD 1Add
BLAST
Repeati196 – 24449WD 2Add
BLAST
Repeati251 – 29242WD 3Add
BLAST
Repeati295 – 33440WD 4Add
BLAST
Repeati347 – 39549WD 5Add
BLAST
Repeati415 – 45440WD 6Add
BLAST
Repeati457 – 49236WD 7Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili90 – 12334UniRule annotationAdd
BLAST

Domaini

Dimerization mediated by the LisH domain may be required to activate dynein.UniRule annotation

Sequence similaritiesi

Belongs to the WD repeat LIS1/nudF family.UniRule annotation
Contains 1 LisH domain.UniRule annotation
Contains 7 WD repeats.UniRule annotation

Keywords - Domaini

Coiled coil, Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00820000127926.
InParanoidiP39946.
KOiK16794.
OMAiSIGICLM.
OrthoDBiEOG715QD8.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
HAMAPiMF_03141. lis1.
InterProiIPR017252. Dynein_regulator_LIS1.
IPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 4 hits.
[Graphical view]
PIRSFiPIRSF037647. Dynein_regulator_Lis1. 1 hit.
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39946-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNWQQQLPL TDTQKNELDK SVLRYLNWNY KQTVRHEHAQ DYESVRHAIV
60 70 80 90 100
TLSGFLLQES VDRQEFISNN DTSNESMVDI DELLLPKKWN SIVRLQKKII
110 120 130 140 150
ELEQNTETLV SQIKDLNTQV SELAQFKPTT SNGTSAHNVL KWIPRNLPSC
160 170 180 190 200
LINVESSVTS VKLHPNLPIV FVATDHGKLY AFDLFNYTIP LASLQSHTKA
210 220 230 240 250
ITSMDVLFTN YTNSSKKNYL VIVTASKDLQ IHVFKWVSEE CKFQQIRSLL
260 270 280 290 300
GHEHIVSAVK IWQKNNDVHI ASCSRDQTVK IWDFHNGWSL KTFQPHSQWV
310 320 330 340 350
RSIDVLGDYI ISGSHDTTLR LTHWPSGNGL SVGTGHEFPI EKVKFIHFIE
360 370 380 390 400
DSPEIRFRTP STDRYKNWGM QYCVSASRDR TIKIWEIPLP TLMAHRAPIP
410 420 430 440 450
NPTDSNFRCV LTLKGHLSWV RDISIRGQYL FSCADDKSVR CWDLNTGQCL
460 470 480 490
HVWEKLHTGF VNCLDLDVDF DSNVTPRQMM VTGGLDCKSN VFMR
Length:494
Mass (Da):56,901
Last modified:October 1, 1996 - v2
Checksum:iE667E4BA7602FB3B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U16827 Genomic DNA. Translation: AAB00685.1.
X89633 Genomic DNA. Translation: CAA61775.1.
Z75177 Genomic DNA. Translation: CAA99493.1.
BK006948 Genomic DNA. Translation: DAA11035.1.
PIRiS67166.
RefSeqiNP_014912.1. NM_001183688.1.

Genome annotation databases

EnsemblFungiiYOR269W; YOR269W; YOR269W.
GeneIDi854443.
KEGGisce:YOR269W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U16827 Genomic DNA. Translation: AAB00685.1.
X89633 Genomic DNA. Translation: CAA61775.1.
Z75177 Genomic DNA. Translation: CAA99493.1.
BK006948 Genomic DNA. Translation: DAA11035.1.
PIRiS67166.
RefSeqiNP_014912.1. NM_001183688.1.

3D structure databases

ProteinModelPortaliP39946.
SMRiP39946. Positions 141-493.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34658. 147 interactions.
DIPiDIP-1485N.
IntActiP39946. 9 interactions.
MINTiMINT-399947.

Proteomic databases

MaxQBiP39946.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR269W; YOR269W; YOR269W.
GeneIDi854443.
KEGGisce:YOR269W.

Organism-specific databases

EuPathDBiFungiDB:YOR269W.
SGDiS000005795. PAC1.

Phylogenomic databases

GeneTreeiENSGT00820000127926.
InParanoidiP39946.
KOiK16794.
OMAiSIGICLM.
OrthoDBiEOG715QD8.

Enzyme and pathway databases

BioCyciYEAST:G3O-33759-MONOMER.

Miscellaneous databases

NextBioi976691.
PROiP39946.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
HAMAPiMF_03141. lis1.
InterProiIPR017252. Dynein_regulator_LIS1.
IPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 4 hits.
[Graphical view]
PIRSFiPIRSF037647. Dynein_regulator_Lis1. 1 hit.
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Saccharomyces cerevisiae genes required in the absence of the CIN8-encoded spindle motor act in functionally diverse mitotic pathways."
    Geiser J.R., Schott E.J., Kingsbury T.J., Cole N.B., Totis L.J., Bhattacharyya G., He L., Hoyt M.A.
    Mol. Biol. Cell 8:1035-1050(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "DNA sequence analysis of the VPH1-SNF2 region on chromosome XV of Saccharomyces cerevisiae."
    Cheret G., Bernardi A., Sor F.J.
    Yeast 12:1059-1064(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Sequencing analysis of a 36.8 kb fragment of yeast chromosome XV reveals 26 open reading frames including SEC63, CDC31, SUG2, GCD1, RBL2, PNT1, PAC1 and VPH1."
    Poirey R., Jauniaux J.-C.
    Yeast 13:483-487(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "The role of the lissencephaly protein Pac1 during nuclear migration in budding yeast."
    Lee W.-L., Oberle J.R., Cooper J.A.
    J. Cell Biol. 160:355-364(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  7. "NudEL targets dynein to microtubule ends through LIS1."
    Li J., Lee W.-L., Cooper J.A.
    Nat. Cell Biol. 7:686-690(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SELF-ASSOCIATION, INTERACTION WITH NDL1, SUBCELLULAR LOCATION.
  8. "Motor- and tail-dependent targeting of dynein to microtubule plus ends and the cell cortex."
    Markus S.M., Punch J.J., Lee W.L.
    Curr. Biol. 19:196-205(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiLIS1_YEAST
AccessioniPrimary (citable) accession number: P39946
Secondary accession number(s): D6W2W9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 1, 1996
Last modified: May 11, 2016
This is version 149 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.