P39944 (UBP5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 5
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 5
Ubiquitin thioesterase 5
Ubiquitin-specific-processing protease 5

Gene names

Name:	UBP5
Ordered Locus Names:	YER144C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	805 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Miscellaneous	Present with 300 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the peptidase C19 family. Contains 1 rhodanese domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein deubiquitination Traceable author statement PubMed 8982460. Source: SGD ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cellular bud neck Inferred from direct assay PubMed 17145966. Source: SGD incipient cellular bud site Inferred from direct assay PubMed 17145966. Source: SGD
Molecular_function	ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro ubiquitin-specific protease activity Traceable author statement PubMed 8982460. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 805	805	Ubiquitin carboxyl-terminal hydrolase 5		PRO_0000080590
Regions
	Domain	159 – 283	125	Rhodanese
Sites
	Active site	455	1	Nucleophile By similarity
	Active site	761	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P39944 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 884054A66370DFF7	FASTA	805	92,261
	10 20 30 40 50 60 MGSEQALSEV VESAKERFGR LRHLVQKFLD DDDVPQECLP LLQECAEIWS SYVDACQDIT 70 80 90 100 110 120 MQAPKEDANR LSKGFLRLNE TAFLYYMIVY TLLEDTLPRL KEFSSNKDQN VRNLYGERIQ 130 140 150 160 170 180 LLHNDPNIER IRNVIENYPK FIQLQTIEPG KLSSMLHFHG DALLLIDVRP RSEFVRAHIK 190 200 210 220 230 240 CKNIICIDPA SFKDSFTDQQ IESVSLITSP HSDITFFSNR DKFKFIILYT DTQLHNNFQQ 250 260 270 280 290 300 RQTRILAKIL SQNSVIKPLS GTKILILENG FSNWVKLGGA YQSSVSETAH LTSSSSTPAF 310 320 330 340 350 360 GSPQVPTGLF NQKSLSPNKD KSMPMVSMNT QPLLTTVQRP QLPLYYSDLP IIPQPSPNRN 370 380 390 400 410 420 SPTVQKFSPH PPTTLSKLNT PSTIQNKANT VERISPDIRA AQAHAYLPPA SNVFSPRIPP 430 440 450 460 470 480 LPQQNLSSSR QTILNNSQVL DLDLIVGLEN IGNCCYMNCI LQCLVGTHDL VRMFLDNTYL 490 500 510 520 530 540 NFINFDSSRG SKGLLAKNFA ILVNNMHRHG AFTPPNVRTI PVQTIQFKKI CGHINPMYSD 550 560 570 580 590 600 SMQQDCQEFC QFLLDGLHED LNQNGSKKHL KQLSDEEERM REKMSIRKAS ALEWERFLLT 610 620 630 640 650 660 DFSAIIDLFQ GQYASRLQCQ VCEHTSTTYQ TFSVLSVPVP RVKTCNILDC FREFTKCERL 670 680 690 700 710 720 GVDEQWSCPK CLKKQPSTKQ LKITRLPKKL IINLKRFDNQ MNKNNVFVQY PYSLDLTPYW 730 740 750 760 770 780 ARDFNHEAIV NEDIPTRGQV PPFRYRLYGV ACHSGSLYGG HYTSYVYKGP KKGWYFFDDS 790 800 LYRPITFSTE FITPSAYVLF YERIF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"UBP5 encodes a putative yeast ubiquitin-specific protease that is related to the human Tre-2 oncogene product." Xiao W., Fontanie T., Tang M. Yeast 10:1497-1502(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U10082 Genomic DNA. Translation: AAC48928.1. U18917 Genomic DNA. Translation: AAB64671.1. BK006939 Genomic DNA. Translation: DAA07805.1.
PIR	S50277.
RefSeq	NP_011071.3. NM_001179034.3.
3D structure databases
ProteinModelPortal	P39944.
SMR	P39944. Positions 445-803.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1716N.
IntAct	P39944. 7 interactions.
MINT	MINT-409937.
STRING	4932.YER144C.
Protein family/group databases
MEROPS	C19.006.
Proteomic databases
PaxDb	P39944.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YER144C; YER144C; YER144C.
GeneID	856887.
KEGG	sce:YER144C.
Organism-specific databases
CYGD	YER144c.
SGD	S000000946. UBP5.
Phylogenomic databases
eggNOG	COG5533.
GeneTree	ENSGT00670000097591.
HOGENOM	HOG000248489.
KO	K11839.
OrthoDB	EOG4HX88F.
Enzyme and pathway databases
BioCyc	YEAST:G3O-30305-MONOMER.
Gene expression databases
Genevestigator	P39944.
GermOnline	YER144C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.40.250.10. 1 hit.
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. IPR001763. Rhodanese-like_dom. [Graphical view]
Pfam	PF00581. Rhodanese. 1 hit. PF00443. UCH. 1 hit. [Graphical view]
SMART	SM00450. RHOD. 1 hit. [Graphical view]
SUPFAM	SSF52821. Rhodanese-like. 1 hit.
PROSITE	PS50206. RHODANESE_3. 1 hit. PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	983284.

Entry information

Entry name

UBP5_YEAST

Accession

Primary (citable) accession number: P39944
Secondary accession number(s): D3DM51

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 29, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program