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Protein

Ubiquitin carboxyl-terminal hydrolase 5

Gene

UBP5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei455 – 4551NucleophilePROSITE-ProRule annotation
Active sitei761 – 7611Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. ubiquitin-specific protease activity Source: SGD

GO - Biological processi

  1. protein deubiquitination Source: SGD
  2. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-30305-MONOMER.

Protein family/group databases

MEROPSiC19.006.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 5 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 5
Ubiquitin thioesterase 5
Ubiquitin-specific-processing protease 5
Gene namesi
Name:UBP5
Ordered Locus Names:YER144C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome V

Organism-specific databases

CYGDiYER144c.
SGDiS000000946. UBP5.

Subcellular locationi

GO - Cellular componenti

  1. cellular bud neck Source: SGD
  2. incipient cellular bud site Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 805805Ubiquitin carboxyl-terminal hydrolase 5PRO_0000080590Add
BLAST

Proteomic databases

MaxQBiP39944.
PaxDbiP39944.

Expressioni

Gene expression databases

GenevestigatoriP39944.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
HOF1Q050803EBI-19847,EBI-5412

Protein-protein interaction databases

BioGridi36893. 30 interactions.
DIPiDIP-1716N.
IntActiP39944. 7 interactions.
MINTiMINT-409937.
STRINGi4932.YER144C.

Structurei

3D structure databases

ProteinModelPortaliP39944.
SMRiP39944. Positions 445-803.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini159 – 283125RhodanesePROSITE-ProRule annotationAdd
BLAST
Domaini446 – 804359USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 rhodanese domain.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiCOG5533.
GeneTreeiENSGT00780000121880.
HOGENOMiHOG000248489.
InParanoidiP39944.
KOiK11839.
OMAiKGISANE.
OrthoDBiEOG7R2BSX.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
InterProiIPR001394. Peptidase_C19_UCH.
IPR001763. Rhodanese-like_dom.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00581. Rhodanese. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view]
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39944-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSEQALSEV VESAKERFGR LRHLVQKFLD DDDVPQECLP LLQECAEIWS
60 70 80 90 100
SYVDACQDIT MQAPKEDANR LSKGFLRLNE TAFLYYMIVY TLLEDTLPRL
110 120 130 140 150
KEFSSNKDQN VRNLYGERIQ LLHNDPNIER IRNVIENYPK FIQLQTIEPG
160 170 180 190 200
KLSSMLHFHG DALLLIDVRP RSEFVRAHIK CKNIICIDPA SFKDSFTDQQ
210 220 230 240 250
IESVSLITSP HSDITFFSNR DKFKFIILYT DTQLHNNFQQ RQTRILAKIL
260 270 280 290 300
SQNSVIKPLS GTKILILENG FSNWVKLGGA YQSSVSETAH LTSSSSTPAF
310 320 330 340 350
GSPQVPTGLF NQKSLSPNKD KSMPMVSMNT QPLLTTVQRP QLPLYYSDLP
360 370 380 390 400
IIPQPSPNRN SPTVQKFSPH PPTTLSKLNT PSTIQNKANT VERISPDIRA
410 420 430 440 450
AQAHAYLPPA SNVFSPRIPP LPQQNLSSSR QTILNNSQVL DLDLIVGLEN
460 470 480 490 500
IGNCCYMNCI LQCLVGTHDL VRMFLDNTYL NFINFDSSRG SKGLLAKNFA
510 520 530 540 550
ILVNNMHRHG AFTPPNVRTI PVQTIQFKKI CGHINPMYSD SMQQDCQEFC
560 570 580 590 600
QFLLDGLHED LNQNGSKKHL KQLSDEEERM REKMSIRKAS ALEWERFLLT
610 620 630 640 650
DFSAIIDLFQ GQYASRLQCQ VCEHTSTTYQ TFSVLSVPVP RVKTCNILDC
660 670 680 690 700
FREFTKCERL GVDEQWSCPK CLKKQPSTKQ LKITRLPKKL IINLKRFDNQ
710 720 730 740 750
MNKNNVFVQY PYSLDLTPYW ARDFNHEAIV NEDIPTRGQV PPFRYRLYGV
760 770 780 790 800
ACHSGSLYGG HYTSYVYKGP KKGWYFFDDS LYRPITFSTE FITPSAYVLF

YERIF
Length:805
Mass (Da):92,261
Last modified:January 31, 1995 - v1
Checksum:i884054A66370DFF7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10082 Genomic DNA. Translation: AAC48928.1.
U18917 Genomic DNA. Translation: AAB64671.1.
BK006939 Genomic DNA. Translation: DAA07805.1.
PIRiS50277.
RefSeqiNP_011071.3. NM_001179034.3.

Genome annotation databases

EnsemblFungiiYER144C; YER144C; YER144C.
GeneIDi856887.
KEGGisce:YER144C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10082 Genomic DNA. Translation: AAC48928.1.
U18917 Genomic DNA. Translation: AAB64671.1.
BK006939 Genomic DNA. Translation: DAA07805.1.
PIRiS50277.
RefSeqiNP_011071.3. NM_001179034.3.

3D structure databases

ProteinModelPortaliP39944.
SMRiP39944. Positions 445-803.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36893. 30 interactions.
DIPiDIP-1716N.
IntActiP39944. 7 interactions.
MINTiMINT-409937.
STRINGi4932.YER144C.

Protein family/group databases

MEROPSiC19.006.

Proteomic databases

MaxQBiP39944.
PaxDbiP39944.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER144C; YER144C; YER144C.
GeneIDi856887.
KEGGisce:YER144C.

Organism-specific databases

CYGDiYER144c.
SGDiS000000946. UBP5.

Phylogenomic databases

eggNOGiCOG5533.
GeneTreeiENSGT00780000121880.
HOGENOMiHOG000248489.
InParanoidiP39944.
KOiK11839.
OMAiKGISANE.
OrthoDBiEOG7R2BSX.

Enzyme and pathway databases

BioCyciYEAST:G3O-30305-MONOMER.

Miscellaneous databases

NextBioi983284.

Gene expression databases

GenevestigatoriP39944.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
InterProiIPR001394. Peptidase_C19_UCH.
IPR001763. Rhodanese-like_dom.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00581. Rhodanese. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view]
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "UBP5 encodes a putative yeast ubiquitin-specific protease that is related to the human Tre-2 oncogene product."
    Xiao W., Fontanie T., Tang M.
    Yeast 10:1497-1502(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiUBP5_YEAST
AccessioniPrimary (citable) accession number: P39944
Secondary accession number(s): D3DM51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 1995
Last sequence update: January 31, 1995
Last modified: February 3, 2015
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 300 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.