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P39944

- UBP5_YEAST

UniProt

P39944 - UBP5_YEAST

Protein

Ubiquitin carboxyl-terminal hydrolase 5

Gene

UBP5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei455 – 4551NucleophilePROSITE-ProRule annotation
    Active sitei761 – 7611Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. ubiquitin-specific protease activity Source: SGD

    GO - Biological processi

    1. protein deubiquitination Source: SGD
    2. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30305-MONOMER.

    Protein family/group databases

    MEROPSiC19.006.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 5 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 5
    Ubiquitin thioesterase 5
    Ubiquitin-specific-processing protease 5
    Gene namesi
    Name:UBP5
    Ordered Locus Names:YER144C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    CYGDiYER144c.
    SGDiS000000946. UBP5.

    Subcellular locationi

    GO - Cellular componenti

    1. cellular bud neck Source: SGD
    2. incipient cellular bud site Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 805805Ubiquitin carboxyl-terminal hydrolase 5PRO_0000080590Add
    BLAST

    Proteomic databases

    MaxQBiP39944.
    PaxDbiP39944.

    Expressioni

    Gene expression databases

    GenevestigatoriP39944.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    HOF1Q050803EBI-19847,EBI-5412

    Protein-protein interaction databases

    BioGridi36893. 29 interactions.
    DIPiDIP-1716N.
    IntActiP39944. 7 interactions.
    MINTiMINT-409937.
    STRINGi4932.YER144C.

    Structurei

    3D structure databases

    ProteinModelPortaliP39944.
    SMRiP39944. Positions 445-803.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini159 – 283125RhodanesePROSITE-ProRule annotationAdd
    BLAST
    Domaini446 – 804359USPAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 1 rhodanese domain.PROSITE-ProRule annotation
    Contains 1 USP domain.Curated

    Phylogenomic databases

    eggNOGiCOG5533.
    GeneTreeiENSGT00750000117363.
    HOGENOMiHOG000248489.
    KOiK11839.
    OMAiKGISANE.
    OrthoDBiEOG7R2BSX.

    Family and domain databases

    Gene3Di3.40.250.10. 1 hit.
    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR001763. Rhodanese-like_dom.
    IPR028889. UCH/PAN2.
    [Graphical view]
    PfamiPF00581. Rhodanese. 1 hit.
    PF00443. UCH. 1 hit.
    [Graphical view]
    SMARTiSM00450. RHOD. 1 hit.
    [Graphical view]
    SUPFAMiSSF52821. SSF52821. 1 hit.
    PROSITEiPS50206. RHODANESE_3. 1 hit.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P39944-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSEQALSEV VESAKERFGR LRHLVQKFLD DDDVPQECLP LLQECAEIWS    50
    SYVDACQDIT MQAPKEDANR LSKGFLRLNE TAFLYYMIVY TLLEDTLPRL 100
    KEFSSNKDQN VRNLYGERIQ LLHNDPNIER IRNVIENYPK FIQLQTIEPG 150
    KLSSMLHFHG DALLLIDVRP RSEFVRAHIK CKNIICIDPA SFKDSFTDQQ 200
    IESVSLITSP HSDITFFSNR DKFKFIILYT DTQLHNNFQQ RQTRILAKIL 250
    SQNSVIKPLS GTKILILENG FSNWVKLGGA YQSSVSETAH LTSSSSTPAF 300
    GSPQVPTGLF NQKSLSPNKD KSMPMVSMNT QPLLTTVQRP QLPLYYSDLP 350
    IIPQPSPNRN SPTVQKFSPH PPTTLSKLNT PSTIQNKANT VERISPDIRA 400
    AQAHAYLPPA SNVFSPRIPP LPQQNLSSSR QTILNNSQVL DLDLIVGLEN 450
    IGNCCYMNCI LQCLVGTHDL VRMFLDNTYL NFINFDSSRG SKGLLAKNFA 500
    ILVNNMHRHG AFTPPNVRTI PVQTIQFKKI CGHINPMYSD SMQQDCQEFC 550
    QFLLDGLHED LNQNGSKKHL KQLSDEEERM REKMSIRKAS ALEWERFLLT 600
    DFSAIIDLFQ GQYASRLQCQ VCEHTSTTYQ TFSVLSVPVP RVKTCNILDC 650
    FREFTKCERL GVDEQWSCPK CLKKQPSTKQ LKITRLPKKL IINLKRFDNQ 700
    MNKNNVFVQY PYSLDLTPYW ARDFNHEAIV NEDIPTRGQV PPFRYRLYGV 750
    ACHSGSLYGG HYTSYVYKGP KKGWYFFDDS LYRPITFSTE FITPSAYVLF 800
    YERIF 805
    Length:805
    Mass (Da):92,261
    Last modified:February 1, 1995 - v1
    Checksum:i884054A66370DFF7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U10082 Genomic DNA. Translation: AAC48928.1.
    U18917 Genomic DNA. Translation: AAB64671.1.
    BK006939 Genomic DNA. Translation: DAA07805.1.
    PIRiS50277.
    RefSeqiNP_011071.3. NM_001179034.3.

    Genome annotation databases

    EnsemblFungiiYER144C; YER144C; YER144C.
    GeneIDi856887.
    KEGGisce:YER144C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U10082 Genomic DNA. Translation: AAC48928.1 .
    U18917 Genomic DNA. Translation: AAB64671.1 .
    BK006939 Genomic DNA. Translation: DAA07805.1 .
    PIRi S50277.
    RefSeqi NP_011071.3. NM_001179034.3.

    3D structure databases

    ProteinModelPortali P39944.
    SMRi P39944. Positions 445-803.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36893. 29 interactions.
    DIPi DIP-1716N.
    IntActi P39944. 7 interactions.
    MINTi MINT-409937.
    STRINGi 4932.YER144C.

    Protein family/group databases

    MEROPSi C19.006.

    Proteomic databases

    MaxQBi P39944.
    PaxDbi P39944.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YER144C ; YER144C ; YER144C .
    GeneIDi 856887.
    KEGGi sce:YER144C.

    Organism-specific databases

    CYGDi YER144c.
    SGDi S000000946. UBP5.

    Phylogenomic databases

    eggNOGi COG5533.
    GeneTreei ENSGT00750000117363.
    HOGENOMi HOG000248489.
    KOi K11839.
    OMAi KGISANE.
    OrthoDBi EOG7R2BSX.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30305-MONOMER.

    Miscellaneous databases

    NextBioi 983284.

    Gene expression databases

    Genevestigatori P39944.

    Family and domain databases

    Gene3Di 3.40.250.10. 1 hit.
    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR001763. Rhodanese-like_dom.
    IPR028889. UCH/PAN2.
    [Graphical view ]
    Pfami PF00581. Rhodanese. 1 hit.
    PF00443. UCH. 1 hit.
    [Graphical view ]
    SMARTi SM00450. RHOD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52821. SSF52821. 1 hit.
    PROSITEi PS50206. RHODANESE_3. 1 hit.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "UBP5 encodes a putative yeast ubiquitin-specific protease that is related to the human Tre-2 oncogene product."
      Xiao W., Fontanie T., Tang M.
      Yeast 10:1497-1502(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiUBP5_YEAST
    AccessioniPrimary (citable) accession number: P39944
    Secondary accession number(s): D3DM51
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 300 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3