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Protein

Transcription corepressor MIG3

Gene

MIG3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-binding transcriptional repressor involved in response to toxic agents such as ribonucleotide reductase inhibitor, hydroxyurea (HU).1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri17 – 3923C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri45 – 6925C2H2-type 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: SGD
  • cellular response to UV Source: SGD
  • negative regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage Source: SGD
  • regulation of transcription from RNA polymerase II promoter by glucose Source: SGD
  • response to ethanol Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-30209-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription corepressor MIG3
Alternative name(s):
Multicopy inhibitor of growth protein 3
Gene namesi
Name:MIG3
Ordered Locus Names:YER028C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER028C.
SGDiS000000830. MIG3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 394394Transcription corepressor MIG3PRO_0000046886Add
BLAST

Post-translational modificationi

Phosphorylated during genotoxic stress. DNA damage induces phosphorylation by SNF1 or the MEC1 pathway and leads to its inactivation, which allows induction of damage response genes.1 Publication

Keywords - PTMi

Phosphoprotein

Expressioni

Inductioni

Down-regulated at low calcium levels.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi36763. 14 interactions.
DIPiDIP-2545N.
IntActiP39943. 3 interactions.
MINTiMINT-426309.

Structurei

3D structure databases

ProteinModelPortaliP39943.
SMRiP39943. Positions 22-70.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi163 – 296134Ser-richAdd
BLAST

Sequence similaritiesi

Contains 2 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri17 – 3923C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri45 – 6925C2H2-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00550000074455.
InParanoidiP39943.
KOiK09467.
OrthoDBiEOG7K0ZMS.

Family and domain databases

Gene3Di3.30.160.60. 2 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 2 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39943-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNYLRDRFPP DNDQRPFRCE ICSRGFHRLE HKKRHGRTHT GEKPHKCTVQ
60 70 80 90 100
GCPKSFSRSD ELKRHLRTHT KGVQRRRIKS KGSRKTVVNT ATAAPTTFNE
110 120 130 140 150
NTGVSLTGIG QSKVPPILIS VAQNCDDVNI RNTGNNNGIV ETQAPAILVP
160 170 180 190 200
VINIPNDPHP IPSSLSTTSI TSIASVYPST SPFQYLKSGF PEDPASTPYV
210 220 230 240 250
HSSGSSLALG ELSSNSSIFS KSRRNLAAMS GPDSLSSSKN QSSASLLSQT
260 270 280 290 300
SHPSKSFSRP PTDLSPLRRI MPSVNTGDME ISRTVSVSSS SSSLTSVTYD
310 320 330 340 350
DTAAKDMGMG IFFDRPPVTQ KACRSNHKYK VNAVSRGRQH ERAQFHISGD
360 370 380 390
DEDSNVHRQE SRASNTSPNV SLPPIKSILR QIDNFNSAPS YFSK
Length:394
Mass (Da):43,119
Last modified:February 1, 1995 - v1
Checksum:i5218D1A0F117BEBB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18778 Genomic DNA. Translation: AAB64561.1.
BK006939 Genomic DNA. Translation: DAA07681.1.
PIRiS50486.
RefSeqiNP_010945.1. NM_001178919.1.

Genome annotation databases

EnsemblFungiiYER028C; YER028C; YER028C.
GeneIDi856750.
KEGGisce:YER028C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18778 Genomic DNA. Translation: AAB64561.1.
BK006939 Genomic DNA. Translation: DAA07681.1.
PIRiS50486.
RefSeqiNP_010945.1. NM_001178919.1.

3D structure databases

ProteinModelPortaliP39943.
SMRiP39943. Positions 22-70.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36763. 14 interactions.
DIPiDIP-2545N.
IntActiP39943. 3 interactions.
MINTiMINT-426309.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER028C; YER028C; YER028C.
GeneIDi856750.
KEGGisce:YER028C.

Organism-specific databases

EuPathDBiFungiDB:YER028C.
SGDiS000000830. MIG3.

Phylogenomic databases

GeneTreeiENSGT00550000074455.
InParanoidiP39943.
KOiK09467.
OrthoDBiEOG7K0ZMS.

Enzyme and pathway databases

BioCyciYEAST:G3O-30209-MONOMER.

Miscellaneous databases

PROiP39943.

Family and domain databases

Gene3Di3.30.160.60. 2 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 2 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Genome-wide analysis of yeast transcription upon calcium shortage."
    Lombardia L.J., Becerra M., Rodriguez-Belmonte E., Hauser N.C., Cerdan M.E.
    Cell Calcium 32:83-91(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. "The protein kinase Snf1 is required for tolerance to the ribonucleotide reductase inhibitor hydroxyurea."
    Dubacq C., Chevalier A., Mann C.
    Mol. Cell. Biol. 24:2560-2572(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION.

Entry informationi

Entry nameiMIG3_YEAST
AccessioniPrimary (citable) accession number: P39943
Secondary accession number(s): D3DLS7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 8, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.