Reviewed,
UniProtKB/Swiss-Prot P39940 (RSP5_YEAST)
Last modified
June 16, 2009.
Version 92.
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: E3 ubiquitin-protein ligase RSP5 EC=6.3.2.- Alternative name(s): Reverses SPT-phenotype protein 5 | ||||||||
| Gene names |
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| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||||||
| Taxonomic identifier | 4932 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 809 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Component of a RSP5 ubiquitin ligase complex which specifies polyubiquitination and intracellular trafficking of the general amino acid permease GAP1 as well as other cell surface proteins like GAP1, FUR4, MAL61, PMA1 and STE2. The RSP5-BUL1/2 complex is also necessary for the heat-shock element (HSE)-mediated gene expression, nitrogen starvation GLN3-dependent transcription, pressure-induced differential regulation of the two tryptophan permeases TAT1 and TAT2 and sorting efficiency into multivesicular bodies. Also acts on RBP1. Plays a role in tolerance to o-dinitrobenzene. Ref.4 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.15 Ref.16 Ref.18 |
| Pathway | |
| Subunit structure | Component of the RSP5-BUL1/2 ubiquitin ligase complex composed of at least RSP5 and BUL1 or BUL2. Forms also a ternary complex with RUP1 and UBP2. The WW domains interact with the singular VPEY sequence in RCR1. Interacts with HSE1, ROG3 and ROD1. Ref.7 Ref.9 Ref.15 Ref.18 Ref.6 Ref.14 Ref.17 |
| Subcellular location | |
| Post-translational modification | The ubiquitination appears to be the result of an intramolecular transfer of ubiquitin. |
| Miscellaneous | A cysteine residue is required for ubiquitin-thioester formation. |
| Sequence similarities | Contains 1 C2 domain. Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain. Contains 3 WW domains. |
Ontologies
Binary interactions
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 809 | 809 | E3 ubiquitin-protein ligase RSP5 | PRO_0000120335 | |||||
Regions | |||||||||
| Domain | 1 – 88 | 88 | C2 | ||||||
| Domain | 229 – 262 | 34 | WW 1 | ||||||
| Domain | 331 – 364 | 34 | WW 2 | ||||||
| Domain | 387 – 420 | 34 | WW 3 | ||||||
| Domain | 705 – 809 | 105 | HECT | ||||||
| Compositional bias | 315 – 322 | 8 | Poly-Ala | ||||||
Sites | |||||||||
| Active site | 777 | 1 | Glycyl thioester intermediate | ||||||
Experimental info | |||||||||
| Mutagenesis | 733 | 1 | L → S in RSP5-1; impairs ubiquitin-thioester formation and catalysis of substrate ubiquitination. Ref.8 | ||||||
| Mutagenesis | 777 | 1 | C → A: Loss of ubiquitination. Ref.8 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W.  Davis R.W.Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972. |
| [2] | Winston F. Unpublished observations (FEB-1993) Cited for: IDENTIFICATION. |
| [3] | "NPI1, an essential yeast gene involved in induced degradation of Gap1 and Fur4 permeases, encodes the Rsp5 ubiquitin-protein ligase." Hein C., Springael J.-Y., Volland C., Haguenauer-Tsapis R., Andre B. Mol. Microbiol. 18:77-87(1995) [PubMed: 8596462] [Abstract] Cited for: CHARACTERIZATION. Strain: Sigma 1278B. |
| [4] | "A family of proteins structurally and functionally related to the E6-AP ubiquitin-protein ligase." Huibregtse J.M., Scheffner M., Beaudenon S., Howley P.M. Proc. Natl. Acad. Sci. U.S.A. 92:2563-2567(1995) [PubMed: 7708685] [Abstract] Cited for: FUNCTION. |
| [5] | Erratum Huibregtse J.M., Scheffner M., Beaudenon S., Howley P.M. Proc. Natl. Acad. Sci. U.S.A. 92:5249-5249(1995) [PubMed: 7761480] [Abstract] |
| [6] | "Bul1, a new protein that binds to the Rsp5 ubiquitin ligase in Saccharomyces cerevisiae." Yashiroda H., Oguchi T., Yasuda Y., Toh-e A., Kikuchi Y. Mol. Cell. Biol. 16:3255-3263(1996) [PubMed: 8668140] [Abstract] Cited for: INTERACTION WITH BUL1. |
| [7] | "The PY-motif of Bul1 protein is essential for growth of Saccharomyces cerevisiae under various stress conditions." Yashiroda H., Kaida D., Toh-e A., Kikuchi Y. Gene 225:39-46(1998) [PubMed: 9931424] [Abstract] Cited for: FUNCTION, INTERACTION WITH BUL1 AND BUL2. |
| [8] | "Functional domains of the rsp5 ubiquitin-protein ligase." Wang G., Yang J., Huibregtse J.M. Mol. Cell. Biol. 19:342-352(1999) [PubMed: 9858558] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF LEU-733 AND CYS-777. Strain: S288c / FY56. |
| [9] | "PY motifs of Rod1 are required for binding to Rsp5 and for drug resistance." Andoh T., Hirata Y., Kikuchi A. FEBS Lett. 525:131-134(2002) [PubMed: 12163175] [Abstract] Cited for: FUNCTION, INTERACTION WITH ROD1 AND ROG3. |
| [10] | "Rsp5-Bul1/2 complex is necessary for the HSE-mediated gene expression in budding yeast." Kaida D., Toh-e A., Kikuchi Y. Biochem. Biophys. Res. Commun. 306:1037-1041(2003) [PubMed: 12821147] [Abstract] Cited for: FUNCTION OF THE RSP5-BUL1/2 COMPLEX. |
| [11] | "Pressure-induced differential regulation of the two tryptophan permeases Tat1 and Tat2 by ubiquitin ligase Rsp5 and its binding proteins, Bul1 and Bul2." Abe F., Iida H. Mol. Cell. Biol. 23:7566-7584(2003) [PubMed: 14560004] [Abstract] Cited for: FUNCTION OF THE RSP5-BUL1/2 COMPLEX. |
| [12] | "NPR1 kinase and RSP5-BUL1/2 ubiquitin ligase control GLN3-dependent transcription in Saccharomyces cerevisiae." Crespo J.L., Helliwell S.B., Wiederkehr C., Demougin P., Fowler B., Primig M., Hall M.N. J. Biol. Chem. 279:37512-37517(2004) [PubMed: 15247235] [Abstract] Cited for: FUNCTION OF THE RSP5-BUL1/2 COMPLEX. |
| [13] | "Ubiquitin-mediated targeting of a mutant plasma membrane ATPase, Pma1-7, to the endosomal/vacuolar system in yeast." Pizzirusso M., Chang A. Mol. Biol. Cell 15:2401-2409(2004) [PubMed: 15020711] [Abstract] Cited for: FUNCTION OF THE RSP5-BUL1/2 COMPLEX. |
| [14] | "Protein-protein interactions of ESCRT complexes in the yeast Saccharomyces cerevisiae." Bowers K., Lottridge J., Helliwell S.B., Goldthwaite L.M., Luzio J.P., Stevens T.H. Traffic 5:194-210(2004) [PubMed: 15086794] [Abstract] Cited for: INTERACTION WITH HSE1. |
| [15] | "The Rsp5 ubiquitin ligase is coupled to and antagonized by the Ubp2 deubiquitinating enzyme." Kee Y., Lyon N., Huibregtse J.M. EMBO J. 24:2414-2424(2005) [PubMed: 15933713] [Abstract] Cited for: FUNCTION, INTERACTION WITH RUP1 AND UBP2. |
| [16] | "Transduction of the nitrogen signal activating Gln3-mediated transcription is independent of Npr1 kinase and Rsp5-Bul1/2 ubiquitin ligase in Saccharomyces cerevisiae." Feller A., Boeckstaens M., Marini A.-M., Dubois E. J. Biol. Chem. 281:28546-28554(2006) [PubMed: 16864574] [Abstract] Cited for: FUNCTION OF THE RSP5-BUL1/2 COMPLEX. |
| [17] | "Peculiar protein-protein interactions of the novel endoplasmic reticulum membrane protein Rcr1 and ubiquitin ligase Rsp5." Imai K., Noda Y., Adachi H., Yoda K. Biosci. Biotechnol. Biochem. 71:249-252(2007) [PubMed: 17213653] [Abstract] Cited for: INTERACTION WITH RCR1. |
| [18] | "Hse1, a component of the yeast Hrs-STAM ubiquitin-sorting complex, associates with ubiquitin peptidases and a ligase to control sorting efficiency into multivesicular bodies." Ren J., Kee Y., Huibregtse J.M., Piper R.C. Mol. Biol. Cell 18:324-335(2007) [PubMed: 17079730] [Abstract] Cited for: FUNCTION, INTERACTION WITH HSE1. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| U18916 Genomic DNA. Translation: AAC03223.1. | |
| PIR | S43217. |
| RefSeq | NP_011051.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1I5H based on UniProtKB Q62940. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP:2238N. |
| IntAct | P39940. 154 interactions. |
Proteomic databases | |
| PeptideAtlas | P39940. |
| PRIDE | P39940. |
Genome annotation databases | |
| Ensembl | YER125W. Saccharomyces cerevisiae. [Contig view] |
| GeneID | 856862. |
| GenomeReviews | Gene locus YER125W in contig U00092_GR. |
| KEGG | sce:YER125W. |
| NMPDR | fig|4932.3.peg.2127. |
Organism-specific databases | |
| CYGD | YER125w. |
| SGD | S000000927. RSP5. |
| Yeast-GFP | Search... |
Phylogenomic databases | |
| HOGENOM | P39940. |
| OMA | P39940. VLQDMEG. |
Gene expression databases | |
| ArrayExpress | P39940. |
| GermOnline | YER125W. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR000008. C2_Ca-dep. IPR018029. C2_membr_targeting. IPR000569. HECT. IPR001202. WW_Rsp5_WWP. [Graphical view] |
| Pfam | PF00168. C2. 1 hit. PF00632. HECT. 1 hit. PF00397. WW. 3 hits. [Graphical view] |
| SMART | SM00239. C2. 1 hit. SM00119. HECTc. 1 hit. SM00456. WW. 3 hits. [Graphical view] |
| PROSITE | PS50004. C2. 1 hit. PS50237. HECT. 1 hit. PS01159. WW_DOMAIN_1. 3 hits. PS50020. WW_DOMAIN_2. 3 hits. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 983216. |
Entry information
| Entry name | RSP5_YEAST | ||||||||
| Accession | Primary (citable) accession number: P39940 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome V Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names |
