P39937 (PAC2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein PAC2

Gene names

Name:	PAC2
Ordered Locus Names:	YER007W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	518 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for viability in the absence of the kinesin-related CIN8 mitotic motor. Seems to be involved in the assembly of alpha-tubulin.
Subcellular location	Cytoplasm › cytoskeleton Probable.
Miscellaneous	Present with 2140 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 CAP-Gly domain. Contains 8 LRR (leucine-rich) repeats.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Microtubule
Domain	Leucine-rich repeat Repeat
Molecular function	Chaperone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein folding Inferred from genetic interaction Ref.1. Source: SGD tubulin complex assembly Inferred from genetic interaction Ref.1. Source: SGD
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW microtubule Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 518	518	Protein PAC2		PRO_0000083526
Regions
	Domain	23 – 67	45	CAP-Gly
	Repeat	153 – 174	22	LRR 1
	Repeat	179 – 201	23	LRR 2
	Repeat	204 – 227	24	LRR 3
	Repeat	229 – 252	24	LRR 4
	Repeat	255 – 276	22	LRR 5
	Repeat	277 – 298	22	LRR 6
	Repeat	299 – 319	21	LRR 7
	Repeat	324 – 345	22	LRR 8

Sequences

	Sequence		Length	Mass (Da)
	P39937 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 3997C60547D04A4A	FASTA	518	59,258
	10 20 30 40 50 60 MTYEIGDRLK IGGYFCTIKF IGVIKPWPSV KAYGVEWDDH SRGKHSGTID DIHYFDVQIP 70 80 90 100 110 120 NSGSFLKESK IKSPGVRRIT FYEALSEKYG GSSNNINDLS IGNKRVEGLG FDELNARNKN 130 140 150 160 170 180 YKKLRKIALR DSDVAILFQN QDELNRVIQN CVNVKDLDLS LNLFTNINSL CEFIEPLKNL 190 200 210 220 230 240 ESLNISQNKL LSGWDNLKEY DLSHIKTLRL SSCGLSYKHI GKLLKSFRTL KMLDLSYNNL 250 260 270 280 290 300 TSAGIQNFEN EIPCTLEELN ISGNNLISFP LFPKNLTLKG LNVSNNQISR APSIAIYSVE 310 320 330 340 350 360 SLDITDNKFK ERSLIDDLNK TFPSLKNIHL SGNEFNYNGN YINVEEQATF YEVLARFDRV 370 380 390 400 410 420 MVLNGSICDV KTRREAEMFF VSKVMNNELS YDTNLPRWSS LIKSYEIDMS KLSFNNERET 430 440 450 460 470 480 RQSLVLKIKI RAGKKPSSDL DYWVLPSFTV RYVKSVICRK LNFDILNVKL FHENSEGMIN 490 500 510 EIKYNFRPIS DFNVVNGDII HVSSPVNNKS IQKVNSPS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Saccharomyces cerevisiae PAC2 functions with CIN1, 2 and 4 in a pathway leading to normal microtubule stability." Hoyt M.A., Macke J.P., Roberts B.T., Geiser J.R. Genetics 146:849-857(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U16814 Genomic DNA. Translation: AAB00683.1. U18778 Genomic DNA. Translation: AAB64540.1. BK006939 Genomic DNA. Translation: DAA07657.1.
PIR	S50465.
RefSeq	NP_010922.1. NM_001178898.1.
3D structure databases
ProteinModelPortal	P39937.
SMR	P39937. Positions 2-81, 94-366.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2342N.
IntAct	P39937. 1 interaction.
MINT	MINT-475566.
STRING	4932.YER007W.
Proteomic databases
PaxDb	P39937.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YER007W; YER007W; YER007W.
GeneID	856724.
KEGG	sce:YER007W.
Organism-specific databases
CYGD	YER007w.
SGD	S000000809. PAC2.
Phylogenomic databases
eggNOG	NOG245706.
GeneTree	ENSGT00530000063405.
OMA	AYGVEWD.
OrthoDB	EOG4WWVTT.
Gene expression databases
Genevestigator	P39937.
GermOnline	YER007W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	2.30.30.190. 1 hit.
InterPro	IPR000938. CAP-Gly_domain. IPR001611. Leu-rich_rpt. [Graphical view]
Pfam	PF01302. CAP_GLY. 1 hit. PF00560. LRR_1. 1 hit. [Graphical view]
SMART	SM01052. CAP_GLY. 1 hit. [Graphical view]
SUPFAM	SSF74924. CAP-Gly_domain. 1 hit.
PROSITE	PS00845. CAP_GLY_1. 1 hit. PS50245. CAP_GLY_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982823.

Entry information

Entry name

PAC2_YEAST

Accession

Primary (citable) accession number: P39937
Secondary accession number(s): D3DLQ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program