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Protein

Eukaryotic initiation factor 4F subunit p150

Gene

TIF4631

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the eIF4F complex, which interacts with the mRNA cap structure and serves as an initial point of assembly for the translation apparatus. Stimulates translation by interaction with polyadenylate-binding protein PAB1, bringing the 5'- and 3'-ends of the mRNA in proximity. The formation of this circular mRNP structure appears to be critical for the synergistic effects of the cap and the poly(A) tail in facilitating translation initiation, recycling of ribosomes, and mRNA stability. TIF4631 is probably essential when TIF4632 is missing.2 Publications

GO - Molecular functioni

  • RNA binding Source: SGD
  • translation initiation factor activity Source: SGD

GO - Biological processi

  • regulation of translation Source: UniProtKB-KW
  • ribosomal large subunit biogenesis Source: SGD
  • stress granule assembly Source: SGD
  • translational initiation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis, Translation regulation

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30861-MONOMER.
ReactomeiR-SCE-1169408. ISG15 antiviral mechanism.
R-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-166208. mTORC1-mediated signalling.
R-SCE-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-SCE-72662. Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S.
R-SCE-72702. Ribosomal scanning and start codon recognition.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic initiation factor 4F subunit p150
Short name:
eIF-4F p150
Short name:
eIF4F p150
Alternative name(s):
eIF4G1
mRNA cap-binding protein complex subunit p150
Gene namesi
Name:TIF4631
Ordered Locus Names:YGR162W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR162W.
SGDiS000003394. TIF4631.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • cytoplasmic mRNA processing body Source: SGD
  • cytoplasmic stress granule Source: SGD
  • eukaryotic translation initiation factor 4F complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi214 – 2174KLRK → AAAA in TIF4631-213; abolishes interaction with PAB1 and inhibits poly(A)-dependent translation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 952952Eukaryotic initiation factor 4F subunit p150PRO_0000213331Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei179 – 1791PhosphothreonineBy similarity
Modified residuei181 – 1811PhosphothreonineCombined sources
Modified residuei299 – 2991PhosphothreonineBy similarity
Modified residuei539 – 5391PhosphoserineBy similarity
Modified residuei883 – 8831PhosphoserineCombined sources
Modified residuei888 – 8881PhosphothreonineCombined sources
Modified residuei908 – 9081PhosphoserineCombined sources
Modified residuei948 – 9481PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP39935.

PTM databases

iPTMnetiP39935.

Interactioni

Subunit structurei

Component of the eIF4F complex, which composition varies with external and internal environmental conditions. It is composed of at least eIF4A (TIF1/TIF2), eIF4E (TIF45) and eIF4G (TIF4631 or TIF4632) (By similarity). Interacts with PAT1 in a RNA-dependent manner.By similarity5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC33P0726015EBI-9002,EBI-150
PAB1P0414716EBI-9002,EBI-12823
TIF2P100817EBI-9002,EBI-9017

Protein-protein interaction databases

BioGridi33414. 145 interactions.
DIPiDIP-985N.
IntActiP39935. 63 interactions.
MINTiMINT-617040.

Structurei

Secondary structure

1
952
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi411 – 4199Combined sources
Helixi427 – 4293Combined sources
Helixi443 – 4453Combined sources
Helixi454 – 46411Combined sources
Helixi471 – 4766Combined sources
Helixi603 – 61513Combined sources
Beta strandi619 – 6213Combined sources
Helixi622 – 63413Combined sources
Helixi635 – 6384Combined sources
Helixi643 – 65816Combined sources
Helixi660 – 6623Combined sources
Helixi663 – 67614Combined sources
Helixi692 – 70918Combined sources
Helixi733 – 75523Combined sources
Helixi761 – 77616Combined sources
Helixi781 – 79818Combined sources
Helixi812 – 82716Combined sources
Helixi834 – 84815Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RF8NMR-B391-488[»]
2VSOX-ray2.60E/F572-854[»]
2VSXX-ray2.80E/F572-854[»]
DisProtiDP00082.
ProteinModelPortaliP39935.
SMRiP39935. Positions 391-488, 577-853.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP39935.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini607 – 850244MIF4GAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni188 – 299112Interaction with PAB1Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi173 – 20028Pro/Ser/Thr-richAdd
BLAST
Compositional biasi375 – 38410Ala/Glu-rich
Compositional biasi488 – 55366Arg/Ser-richAdd
BLAST
Compositional biasi869 – 8724Poly-Glu
Compositional biasi873 – 89927Arg/Ser-richAdd
BLAST

Sequence similaritiesi

Contains 1 MIF4G domain.Curated

Phylogenomic databases

GeneTreeiENSGT00530000063038.
HOGENOMiHOG000065985.
InParanoidiP39935.
KOiK03260.
OMAiATNMFSA.
OrthoDBiEOG092C0NOJ.

Family and domain databases

Gene3Di1.25.40.180. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR022745. eIF4G1_eIF4E-bd.
IPR016021. MIF4-like.
IPR003890. MIF4G-like_typ-3.
[Graphical view]
PfamiPF12152. eIF_4G1. 1 hit.
PF02854. MIF4G. 1 hit.
[Graphical view]
SMARTiSM00543. MIF4G. 1 hit.
[Graphical view]
SUPFAMiSSF101489. SSF101489. 1 hit.
SSF48371. SSF48371. 1 hit.

Sequencei

Sequence statusi: Complete.

P39935-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDETAHPTQ SASKQESAAL KQTGDDQQES QQQRGYTNYN NGSNYTQKKP
60 70 80 90 100
YNSNRPHQQR GGKFGPNRYN NRGNYNGGGS FRGGHMGANS SNVPWTGYYN
110 120 130 140 150
NYPVYYQPQQ MAAAGSAPAN PIPVEEKSPV PTKIEITTKS GEHLDLKEQH
160 170 180 190 200
KAKLQSQERS TVSPQPESKL KETSDSTSTS TPTPTPSTND SKASSEENIS
210 220 230 240 250
EAEKTRRNFI EQVKLRKAAL EKKRKEQLEG SSGNNNIPMK TTPENVEEKG
260 270 280 290 300
SDKPEVTEKT KPAEEKSAEP EVKQETPAEE GEQGEKGQIK EESTPKVLTF
310 320 330 340 350
AERLKLKKQQ KEREEKTEGK ENKEVPVQEE TKSAIESAPV PPSEQVKEET
360 370 380 390 400
EVAETEQSNI DESATTPAIP TKSDEAEAEV EAEAGDAGTK IGLEAEIETT
410 420 430 440 450
TDETDDGTNT VSHILNVLKD ATPIEDVFSF NYPEGIEGPD IKYKKEHVKY
460 470 480 490 500
TYGPTFLLQF KDKLNVKADA EWVQSTASKI VIPPGMGRGN RSRDSGRFGN
510 520 530 540 550
NSSRGHDFRN TSVRNMDDRA NSRTSSKRRS KRMNDDRRSN RSYTSRRDRE
560 570 580 590 600
RGSYRNEEKR EDDKPKEEVA PLVPSANRWV PKFKSKKTEK KLAPDGKTEL
610 620 630 640 650
LDKDEVERKM KSLLNKLTLE MFDAISSEIL AIANISVWET NGETLKAVIE
660 670 680 690 700
QIFLKACDEP HWSSMYAQLC GKVVKELNPD ITDETNEGKT GPKLVLHYLV
710 720 730 740 750
ARCHAEFDKG WTDKLPTNED GTPLEPEMMS EEYYAAASAK RRGLGLVRFI
760 770 780 790 800
GFLYRLNLLT GKMMFECFRR LMKDLTDSPS EETLESVVEL LNTVGEQFET
810 820 830 840 850
DSFRTGQATL EGSQLLDSLF GILDNIIQTA KISSRIKFKL IDIKELRHDK
860 870 880 890 900
NWNSDKKDNG PKTIQQIHEE EERQRQLKNN SRSNSRRTNN SSNRHSFRRD
910 920 930 940 950
APPASKDSFI TTRTYSQRNS QRAPPPKEEP AAPTSTATNM FSALMGESDD

EE
Length:952
Mass (Da):107,101
Last modified:October 1, 1996 - v2
Checksum:i391256802F86118E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti7 – 71H → Q in AAA02757 (PubMed:8336723).Curated
Sequence conflicti37 – 371T → N in AAA02757 (PubMed:8336723).Curated
Sequence conflicti110 – 1101Q → K in AAA02757 (PubMed:8336723).Curated
Sequence conflicti207 – 2071R → K in AAA02757 (PubMed:8336723).Curated
Sequence conflicti361 – 3611D → E in AAA02757 (PubMed:8336723).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L16923 Genomic DNA. Translation: AAA02757.1.
Z72947 Genomic DNA. Translation: CAA97184.1.
AY692973 Genomic DNA. Translation: AAT92992.1.
BK006941 Genomic DNA. Translation: DAA08257.1.
PIRiS64473.
RefSeqiNP_011678.3. NM_001181291.3.

Genome annotation databases

EnsemblFungiiYGR162W; YGR162W; YGR162W.
GeneIDi853071.
KEGGisce:YGR162W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L16923 Genomic DNA. Translation: AAA02757.1.
Z72947 Genomic DNA. Translation: CAA97184.1.
AY692973 Genomic DNA. Translation: AAT92992.1.
BK006941 Genomic DNA. Translation: DAA08257.1.
PIRiS64473.
RefSeqiNP_011678.3. NM_001181291.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RF8NMR-B391-488[»]
2VSOX-ray2.60E/F572-854[»]
2VSXX-ray2.80E/F572-854[»]
DisProtiDP00082.
ProteinModelPortaliP39935.
SMRiP39935. Positions 391-488, 577-853.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33414. 145 interactions.
DIPiDIP-985N.
IntActiP39935. 63 interactions.
MINTiMINT-617040.

PTM databases

iPTMnetiP39935.

Proteomic databases

MaxQBiP39935.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR162W; YGR162W; YGR162W.
GeneIDi853071.
KEGGisce:YGR162W.

Organism-specific databases

EuPathDBiFungiDB:YGR162W.
SGDiS000003394. TIF4631.

Phylogenomic databases

GeneTreeiENSGT00530000063038.
HOGENOMiHOG000065985.
InParanoidiP39935.
KOiK03260.
OMAiATNMFSA.
OrthoDBiEOG092C0NOJ.

Enzyme and pathway databases

BioCyciYEAST:G3O-30861-MONOMER.
ReactomeiR-SCE-1169408. ISG15 antiviral mechanism.
R-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-166208. mTORC1-mediated signalling.
R-SCE-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-SCE-72662. Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S.
R-SCE-72702. Ribosomal scanning and start codon recognition.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

EvolutionaryTraceiP39935.
PROiP39935.

Family and domain databases

Gene3Di1.25.40.180. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR022745. eIF4G1_eIF4E-bd.
IPR016021. MIF4-like.
IPR003890. MIF4G-like_typ-3.
[Graphical view]
PfamiPF12152. eIF_4G1. 1 hit.
PF02854. MIF4G. 1 hit.
[Graphical view]
SMARTiSM00543. MIF4G. 1 hit.
[Graphical view]
SUPFAMiSSF101489. SSF101489. 1 hit.
SSF48371. SSF48371. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiIF4F1_YEAST
AccessioniPrimary (citable) accession number: P39935
Secondary accession number(s): D6VUU6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 165 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 9760 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.