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Protein

Eukaryotic initiation factor 4F subunit p150

Gene

TIF4631

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the eIF4F complex, which interacts with the mRNA cap structure and serves as an initial point of assembly for the translation apparatus. Stimulates translation by interaction with polyadenylate-binding protein PAB1, bringing the 5'- and 3'-ends of the mRNA in proximity. The formation of this circular mRNP structure appears to be critical for the synergistic effects of the cap and the poly(A) tail in facilitating translation initiation, recycling of ribosomes, and mRNA stability. TIF4631 is probably essential when TIF4632 is missing.2 Publications

GO - Molecular functioni

  • RNA binding Source: SGD
  • translation initiation factor activity Source: SGD

GO - Biological processi

  • regulation of translation Source: UniProtKB-KW
  • ribosomal large subunit biogenesis Source: SGD
  • stress granule assembly Source: SGD
  • translational initiation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis, Translation regulation

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30861-MONOMER.
ReactomeiR-SCE-1169408. ISG15 antiviral mechanism.
R-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-166208. mTORC1-mediated signalling.
R-SCE-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-SCE-72662. Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S.
R-SCE-72702. Ribosomal scanning and start codon recognition.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic initiation factor 4F subunit p150
Short name:
eIF-4F p150
Short name:
eIF4F p150
Alternative name(s):
eIF4G1
mRNA cap-binding protein complex subunit p150
Gene namesi
Name:TIF4631
Ordered Locus Names:YGR162W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR162W.
SGDiS000003394. TIF4631.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • cytoplasmic mRNA processing body Source: SGD
  • cytoplasmic stress granule Source: SGD
  • eukaryotic translation initiation factor 4F complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi214 – 217KLRK → AAAA in TIF4631-213; abolishes interaction with PAB1 and inhibits poly(A)-dependent translation. 1 Publication4

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002133311 – 952Eukaryotic initiation factor 4F subunit p150Add BLAST952

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei179PhosphothreonineBy similarity1
Modified residuei181PhosphothreonineCombined sources1
Modified residuei299PhosphothreonineBy similarity1
Modified residuei539PhosphoserineBy similarity1
Modified residuei883PhosphoserineCombined sources1
Modified residuei888PhosphothreonineCombined sources1
Modified residuei908PhosphoserineCombined sources1
Modified residuei948PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP39935.
PRIDEiP39935.

PTM databases

iPTMnetiP39935.

Interactioni

Subunit structurei

Component of the eIF4F complex, which composition varies with external and internal environmental conditions. It is composed of at least eIF4A (TIF1/TIF2), eIF4E (TIF45) and eIF4G (TIF4631 or TIF4632) (By similarity). Interacts with PAT1 in a RNA-dependent manner.By similarity5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC33P0726015EBI-9002,EBI-150
PAB1P0414716EBI-9002,EBI-12823
TIF2P100817EBI-9002,EBI-9017

Protein-protein interaction databases

BioGridi33414. 145 interactors.
DIPiDIP-985N.
IntActiP39935. 63 interactors.
MINTiMINT-617040.

Structurei

Secondary structure

1952
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi411 – 419Combined sources9
Helixi427 – 429Combined sources3
Helixi443 – 445Combined sources3
Helixi454 – 464Combined sources11
Helixi471 – 476Combined sources6
Helixi603 – 615Combined sources13
Beta strandi619 – 621Combined sources3
Helixi622 – 634Combined sources13
Helixi635 – 638Combined sources4
Helixi643 – 658Combined sources16
Helixi660 – 662Combined sources3
Helixi663 – 676Combined sources14
Helixi692 – 709Combined sources18
Helixi733 – 755Combined sources23
Helixi761 – 776Combined sources16
Helixi781 – 798Combined sources18
Helixi812 – 827Combined sources16
Helixi834 – 848Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RF8NMR-B391-488[»]
2VSOX-ray2.60E/F572-854[»]
2VSXX-ray2.80E/F572-854[»]
DisProtiDP00082.
ProteinModelPortaliP39935.
SMRiP39935.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP39935.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini607 – 850MIF4GAdd BLAST244

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni188 – 299Interaction with PAB1Add BLAST112

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi173 – 200Pro/Ser/Thr-richAdd BLAST28
Compositional biasi375 – 384Ala/Glu-rich10
Compositional biasi488 – 553Arg/Ser-richAdd BLAST66
Compositional biasi869 – 872Poly-Glu4
Compositional biasi873 – 899Arg/Ser-richAdd BLAST27

Sequence similaritiesi

Contains 1 MIF4G domain.Curated

Phylogenomic databases

GeneTreeiENSGT00530000063038.
HOGENOMiHOG000065985.
InParanoidiP39935.
KOiK03260.
OMAiATNMFSA.
OrthoDBiEOG092C0NOJ.

Family and domain databases

Gene3Di1.25.40.180. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR022745. eIF4G1_eIF4E-bd.
IPR016021. MIF4-like.
IPR003890. MIF4G-like_typ-3.
[Graphical view]
PfamiPF12152. eIF_4G1. 1 hit.
PF02854. MIF4G. 1 hit.
[Graphical view]
SMARTiSM00543. MIF4G. 1 hit.
[Graphical view]
SUPFAMiSSF101489. SSF101489. 1 hit.
SSF48371. SSF48371. 1 hit.

Sequencei

Sequence statusi: Complete.

P39935-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDETAHPTQ SASKQESAAL KQTGDDQQES QQQRGYTNYN NGSNYTQKKP
60 70 80 90 100
YNSNRPHQQR GGKFGPNRYN NRGNYNGGGS FRGGHMGANS SNVPWTGYYN
110 120 130 140 150
NYPVYYQPQQ MAAAGSAPAN PIPVEEKSPV PTKIEITTKS GEHLDLKEQH
160 170 180 190 200
KAKLQSQERS TVSPQPESKL KETSDSTSTS TPTPTPSTND SKASSEENIS
210 220 230 240 250
EAEKTRRNFI EQVKLRKAAL EKKRKEQLEG SSGNNNIPMK TTPENVEEKG
260 270 280 290 300
SDKPEVTEKT KPAEEKSAEP EVKQETPAEE GEQGEKGQIK EESTPKVLTF
310 320 330 340 350
AERLKLKKQQ KEREEKTEGK ENKEVPVQEE TKSAIESAPV PPSEQVKEET
360 370 380 390 400
EVAETEQSNI DESATTPAIP TKSDEAEAEV EAEAGDAGTK IGLEAEIETT
410 420 430 440 450
TDETDDGTNT VSHILNVLKD ATPIEDVFSF NYPEGIEGPD IKYKKEHVKY
460 470 480 490 500
TYGPTFLLQF KDKLNVKADA EWVQSTASKI VIPPGMGRGN RSRDSGRFGN
510 520 530 540 550
NSSRGHDFRN TSVRNMDDRA NSRTSSKRRS KRMNDDRRSN RSYTSRRDRE
560 570 580 590 600
RGSYRNEEKR EDDKPKEEVA PLVPSANRWV PKFKSKKTEK KLAPDGKTEL
610 620 630 640 650
LDKDEVERKM KSLLNKLTLE MFDAISSEIL AIANISVWET NGETLKAVIE
660 670 680 690 700
QIFLKACDEP HWSSMYAQLC GKVVKELNPD ITDETNEGKT GPKLVLHYLV
710 720 730 740 750
ARCHAEFDKG WTDKLPTNED GTPLEPEMMS EEYYAAASAK RRGLGLVRFI
760 770 780 790 800
GFLYRLNLLT GKMMFECFRR LMKDLTDSPS EETLESVVEL LNTVGEQFET
810 820 830 840 850
DSFRTGQATL EGSQLLDSLF GILDNIIQTA KISSRIKFKL IDIKELRHDK
860 870 880 890 900
NWNSDKKDNG PKTIQQIHEE EERQRQLKNN SRSNSRRTNN SSNRHSFRRD
910 920 930 940 950
APPASKDSFI TTRTYSQRNS QRAPPPKEEP AAPTSTATNM FSALMGESDD

EE
Length:952
Mass (Da):107,101
Last modified:October 1, 1996 - v2
Checksum:i391256802F86118E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7H → Q in AAA02757 (PubMed:8336723).Curated1
Sequence conflicti37T → N in AAA02757 (PubMed:8336723).Curated1
Sequence conflicti110Q → K in AAA02757 (PubMed:8336723).Curated1
Sequence conflicti207R → K in AAA02757 (PubMed:8336723).Curated1
Sequence conflicti361D → E in AAA02757 (PubMed:8336723).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L16923 Genomic DNA. Translation: AAA02757.1.
Z72947 Genomic DNA. Translation: CAA97184.1.
AY692973 Genomic DNA. Translation: AAT92992.1.
BK006941 Genomic DNA. Translation: DAA08257.1.
PIRiS64473.
RefSeqiNP_011678.3. NM_001181291.3.

Genome annotation databases

EnsemblFungiiYGR162W; YGR162W; YGR162W.
GeneIDi853071.
KEGGisce:YGR162W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L16923 Genomic DNA. Translation: AAA02757.1.
Z72947 Genomic DNA. Translation: CAA97184.1.
AY692973 Genomic DNA. Translation: AAT92992.1.
BK006941 Genomic DNA. Translation: DAA08257.1.
PIRiS64473.
RefSeqiNP_011678.3. NM_001181291.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RF8NMR-B391-488[»]
2VSOX-ray2.60E/F572-854[»]
2VSXX-ray2.80E/F572-854[»]
DisProtiDP00082.
ProteinModelPortaliP39935.
SMRiP39935.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33414. 145 interactors.
DIPiDIP-985N.
IntActiP39935. 63 interactors.
MINTiMINT-617040.

PTM databases

iPTMnetiP39935.

Proteomic databases

MaxQBiP39935.
PRIDEiP39935.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR162W; YGR162W; YGR162W.
GeneIDi853071.
KEGGisce:YGR162W.

Organism-specific databases

EuPathDBiFungiDB:YGR162W.
SGDiS000003394. TIF4631.

Phylogenomic databases

GeneTreeiENSGT00530000063038.
HOGENOMiHOG000065985.
InParanoidiP39935.
KOiK03260.
OMAiATNMFSA.
OrthoDBiEOG092C0NOJ.

Enzyme and pathway databases

BioCyciYEAST:G3O-30861-MONOMER.
ReactomeiR-SCE-1169408. ISG15 antiviral mechanism.
R-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-166208. mTORC1-mediated signalling.
R-SCE-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-SCE-72662. Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S.
R-SCE-72702. Ribosomal scanning and start codon recognition.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

EvolutionaryTraceiP39935.
PROiP39935.

Family and domain databases

Gene3Di1.25.40.180. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR022745. eIF4G1_eIF4E-bd.
IPR016021. MIF4-like.
IPR003890. MIF4G-like_typ-3.
[Graphical view]
PfamiPF12152. eIF_4G1. 1 hit.
PF02854. MIF4G. 1 hit.
[Graphical view]
SMARTiSM00543. MIF4G. 1 hit.
[Graphical view]
SUPFAMiSSF101489. SSF101489. 1 hit.
SSF48371. SSF48371. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiIF4F1_YEAST
AccessioniPrimary (citable) accession number: P39935
Secondary accession number(s): D6VUU6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 167 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 9760 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.