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P39929

- SNF7_YEAST

UniProt

P39929 - SNF7_YEAST

Protein

Vacuolar-sorting protein SNF7

Gene

SNF7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Required for the sorting and concentration of proteins resulting in the entry of these proteins into the invaginating vesicles of the multivesicular body (MVB). Acts a component of the ESCRT-III complex, which appears to be critical for late steps in MVB sorting, such as membrane invagination and final cargo sorting and recruitment of late-acting components of the sorting machinery. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complex assemblies. Appears to sequester MVB cargo. Recruits BRO1, which in turn recruits DOA4, which deubiquitinates cargos before their enclosure within MVB vesicles.2 Publications

    GO - Molecular functioni

    1. identical protein binding Source: IntAct
    2. protein binding Source: IntAct

    GO - Biological processi

    1. cellular response to anoxia Source: SGD
    2. intralumenal vesicle formation Source: SGD
    3. late endosome to vacuole transport Source: SGD
    4. protein transport Source: UniProtKB-KW
    5. ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: SGD

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32186-MONOMER.
    ReactomeiREACT_189189. Endosomal Sorting Complex Required For Transport (ESCRT).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vacuolar-sorting protein SNF7
    Alternative name(s):
    DOA4-independent degradation protein 1
    Vacuolar protein-sorting-associated protein 32
    Gene namesi
    Name:SNF7
    Synonyms:DID1, VPS32
    Ordered Locus Names:YLR025W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLR025w.
    SGDiS000004015. SNF7.

    Subcellular locationi

    Cytoplasm 1 Publication. Endosome membrane 1 Publication; Peripheral membrane protein 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. ESCRT III complex Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Endosome, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 240240Vacuolar-sorting protein SNF7PRO_0000211446Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei72 – 721Phosphothreonine2 Publications
    Modified residuei119 – 1191Phosphoserine1 Publication
    Modified residuei193 – 1931Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP39929.
    PaxDbiP39929.
    PeptideAtlasiP39929.

    Expressioni

    Gene expression databases

    GenevestigatoriP39929.

    Interactioni

    Subunit structurei

    Self-associates. Core component of the ESCRT-III complex (endosomal sorting required for transport complex III). ESCRT-III appears to be sequentially assembled as a flat lattice on the endosome membrane and forms a transient 450 kDa complex that contains DID4, oligomerized SNF7, VPS20 and VPS24. SNF7 oligomerization into a membrane-associated filament is nucleated by association of SNF7 with VPS20; the process is terminated through association of VPS24, possibly by capping the SNF7 filament. VPS24 subsequently associates with DID4/VPS2. Interacts with VTA1; the interaction requires DID2. Interacts with BRO1.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself7EBI-17554,EBI-17554
    DID4P361084EBI-17554,EBI-26574
    VPS20Q042723EBI-17554,EBI-28157
    VPS4P529174EBI-17554,EBI-20475

    Protein-protein interaction databases

    BioGridi31299. 105 interactions.
    DIPiDIP-1747N.
    IntActiP39929. 25 interactions.
    MINTiMINT-389266.
    STRINGi4932.YLR025W.

    Structurei

    3D structure databases

    ProteinModelPortaliP39929.
    SMRiP39929. Positions 20-95, 121-175.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi68 – 714Poly-Lys
    Compositional biasi216 – 22813Asp/Glu-rich (acidic)Add
    BLAST

    Sequence similaritiesi

    Belongs to the SNF7 family.Curated

    Phylogenomic databases

    eggNOGiNOG291419.
    GeneTreeiENSGT00390000005006.
    HOGENOMiHOG000209960.
    KOiK12194.
    OMAiENEARIF.
    OrthoDBiEOG72RN9W.

    Family and domain databases

    InterProiIPR005024. Snf7.
    [Graphical view]
    PfamiPF03357. Snf7. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P39929-1 [UniParc]FASTAAdd to Basket

    « Hide

    MWSSLFGWTS SNAKNKESPT KAIVRLREHI NLLSKKQSHL RTQITNQENE    50
    ARIFLTKGNK VMAKNALKKK KTIEQLLSKV EGTMESMEQQ LFSIESANLN 100
    LETMRAMQEG AKAMKTIHSG LDIDKVDETM DEIREQVELG DEISDAISRP 150
    LITGANEVDE DELDEELDML AQENANQETS KIVNNNVNAA PISENKVSLP 200
    SVPSNKIKQS ENSVKDGEEE EDEEDEDEKA LRELQAEMGL 240
    Length:240
    Mass (Da):26,987
    Last modified:February 1, 1995 - v1
    Checksum:i5241A18BB181F0C1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti173 – 1731E → G in AAS56528. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L09751 Genomic DNA. No translation available.
    Z73197 Genomic DNA. Translation: CAA97548.1.
    AY558202 Genomic DNA. Translation: AAS56528.1.
    BK006945 Genomic DNA. Translation: DAA09343.1.
    PIRiS52590.
    RefSeqiNP_013125.1. NM_001181912.1.

    Genome annotation databases

    EnsemblFungiiYLR025W; YLR025W; YLR025W.
    GeneIDi850712.
    KEGGisce:YLR025W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L09751 Genomic DNA. No translation available.
    Z73197 Genomic DNA. Translation: CAA97548.1 .
    AY558202 Genomic DNA. Translation: AAS56528.1 .
    BK006945 Genomic DNA. Translation: DAA09343.1 .
    PIRi S52590.
    RefSeqi NP_013125.1. NM_001181912.1.

    3D structure databases

    ProteinModelPortali P39929.
    SMRi P39929. Positions 20-95, 121-175.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31299. 105 interactions.
    DIPi DIP-1747N.
    IntActi P39929. 25 interactions.
    MINTi MINT-389266.
    STRINGi 4932.YLR025W.

    Proteomic databases

    MaxQBi P39929.
    PaxDbi P39929.
    PeptideAtlasi P39929.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR025W ; YLR025W ; YLR025W .
    GeneIDi 850712.
    KEGGi sce:YLR025W.

    Organism-specific databases

    CYGDi YLR025w.
    SGDi S000004015. SNF7.

    Phylogenomic databases

    eggNOGi NOG291419.
    GeneTreei ENSGT00390000005006.
    HOGENOMi HOG000209960.
    KOi K12194.
    OMAi ENEARIF.
    OrthoDBi EOG72RN9W.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32186-MONOMER.
    Reactomei REACT_189189. Endosomal Sorting Complex Required For Transport (ESCRT).

    Miscellaneous databases

    NextBioi 966772.
    PROi P39929.

    Gene expression databases

    Genevestigatori P39929.

    Family and domain databases

    InterProi IPR005024. Snf7.
    [Graphical view ]
    Pfami PF03357. Snf7. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular and genetic analysis of the SNF7 gene in Saccharomyces cerevisiae."
      Tu J., Vallier L.G., Carlson M.
      Genetics 135:17-23(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "CHMP1 functions as a member of a newly defined family of vesicle trafficking proteins."
      Howard T.L., Stauffer D.R., Degnin C.R., Hollenberg S.M.
      J. Cell Sci. 114:2395-2404(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Escrt-III: an endosome-associated heterooligomeric protein complex required for mvb sorting."
      Babst M., Katzmann D.J., Estepa-Sabal E.J., Meerloo T., Emr S.D.
      Dev. Cell 3:271-282(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN THE ESCRT-III COMPLEX, INTERACTION WITH VPS20, SUBCELLULAR LOCATION.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Protein-protein interactions of ESCRT complexes in the yeast Saccharomyces cerevisiae."
      Bowers K., Lottridge J., Helliwell S.B., Goldthwaite L.M., Luzio J.P., Stevens T.H.
      Traffic 5:194-210(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH BRO1.
    9. "Structural basis for endosomal targeting by the Bro1 domain."
      Kim J., Sitaraman S., Hierro A., Beach B.M., Odorizzi G., Hurley J.H.
      Dev. Cell 8:937-947(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH BRO1.
    10. "Vta1p and Vps46p regulate the membrane association and ATPase activity of Vps4p at the yeast multivesicular body."
      Lottridge J.M., Flannery A.R., Vincelli J.L., Stevens T.H.
      Proc. Natl. Acad. Sci. U.S.A. 103:6202-6207(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH VTA1.
    11. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-72, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    12. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-72, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "Ordered assembly of the ESCRT-III complex on endosomes is required to sequester cargo during MVB formation."
      Teis D., Saksena S., Emr S.D.
      Dev. Cell 15:578-589(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: ASSEMBLY OF THE ESCRT-III COMPLEX.
    14. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-119 AND SER-193, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    15. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    16. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSNF7_YEAST
    AccessioniPrimary (citable) accession number: P39929
    Secondary accession number(s): D6VY27, E9P8V6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 134 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3270 molecules/cell in log phase SD medium.1 Publication

    Caution

    Was originally thought to be a nuclear protein and mutations were shown to prevent full derepression of the SUC2 (invertase) gene.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3