Vacuolar-sorting protein SNF7 - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot P39929 (SNF7_YEAST)

Last modified November 24, 2009. Version 89. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Vacuolar-sorting protein SNF7
Alternative name(s):
    Vacuolar protein-sorting-associated protein 32
    DOA4-independent degradation protein 1

Gene names

Name:	SNF7
Synonyms:	DID1, VPS32
Ordered Locus Names:	YLR025W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	240 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Required for the sorting and concentration of proteins resulting in the entry of these proteins into the invaginating vesicles of the multivesicular body (MVB). Acts a component of the ESCRT-III complex, which appears to be critical for late steps in MVB sorting, such as membrane invagination and final cargo sorting and recruitment of late-acting components of the sorting machinery. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complex assemblies. Appears to sequester MVB cargo. Recruits BRO1, which in turn recruits DOA4, which deubiquitinates cargos before their enclosure within MVB vesicles. Ref.3 Ref.4
Subunit structure	Self-associates. Core component of the ESCRT-III complex (endosomal sorting required for transport complex III). ESCRT-III appears to be sequentially assembled as a flat lattice on the endosome membrane and forms a transient 450 kDa complex that contains DID4, oligomerized SNF7, VPS20 and VPS24. SNF7 oligomerization into a membrane-associated filament is nucleated by association of SNF7 with VPS20; the process is terminated through association of VPS24, possibly by capping the SNF7 filament. VPS24 subsequently associates with DID4/VPS2. Interacts with VTA1; the interaction requires DID2. Interacts with BRO1. Ref.4 Ref.6 Ref.7 Ref.8
Subcellular location	Cytoplasm. Endosome membrane; Peripheral membrane protein.
Miscellaneous	Present with 3270 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the SNF7 family.
Caution	Was originally (Ref.1) thought to be a nuclear protein and mutations were shown to prevent full derepression of the SUC2 (invertase) gene.

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Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Endosome Membrane
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	intralumenal vesicle formation Inferred from mutant phenotype. Source: SGD late endosome to vacuole transport Inferred from mutant phenotype. Source: SGD protein transport Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Ref.6 Inferred by curator. Source: SGD
Cellular component	ESCRT III complex Ref.4 Inferred from direct assay. Source: SGD extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	identical protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
itself		1	EBI-17554,EBI-17554
	P53272	1	EBI-17554,EBI-23284
	Q03049	1	EBI-17554,EBI-35573
ALO1	P54783	1	EBI-17554,EBI-2519
DID2	P69771	1	EBI-17554,EBI-2053489
DID4	P36108	1	EBI-17554,EBI-26574
RIM13	Q03792	1	EBI-17554,EBI-27327
RIM20	Q12033	1	EBI-17554,EBI-38947
VPS24	P36095	1	EBI-17554,EBI-26653
VPS4	P52917	2	EBI-17554,EBI-20475
VPS60	Q03390	1	EBI-17554,EBI-2090142
VTA1	Q06263	1	EBI-17554,EBI-37098

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 240

240

Vacuolar-sorting protein SNF7

PRO_0000211446

Regions

Compositional bias

68 – 71

Poly-Lys

Compositional bias

216 – 228

Asp/Glu-rich (acidic)

Amino acid modifications

Modified residue

Phosphothreonine Ref.9 Ref.10 Ref.13

Modified residue

116

Phosphothreonine Ref.13

Modified residue

119

Phosphoserine Ref.10 Ref.13

Modified residue

193

Phosphoserine Ref.13

Modified residue

204

Phosphoserine Ref.13

Modified residue

213

Phosphoserine Ref.11

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Sequences

Sequence

Length

Mass (Da)

Tools

P39929-1 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 5241A18BB181F0C1
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FASTA

240

26,987

        10         20         30         40         50         60 
MWSSLFGWTS SNAKNKESPT KAIVRLREHI NLLSKKQSHL RTQITNQENE ARIFLTKGNK 

        70         80         90        100        110        120 
VMAKNALKKK KTIEQLLSKV EGTMESMEQQ LFSIESANLN LETMRAMQEG AKAMKTIHSG 

       130        140        150        160        170        180 
LDIDKVDETM DEIREQVELG DEISDAISRP LITGANEVDE DELDEELDML AQENANQETS 

       190        200        210        220        230        240 
KIVNNNVNAA PISENKVSLP SVPSNKIKQS ENSVKDGEEE EDEEDEDEKA LRELQAEMGL

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular and genetic analysis of the SNF7 gene in Saccharomyces cerevisiae." Tu J., Vallier L.G., Carlson M. Genetics 135:17-23(1993) [PubMed: 8224817] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"CHMP1 functions as a member of a newly defined family of vesicle trafficking proteins." Howard T.L., Stauffer D.R., Degnin C.R., Hollenberg S.M. J. Cell Sci. 114:2395-2404(2001) [PubMed: 11559748] [Abstract] Cited for: FUNCTION.
[4]	"Escrt-III: an endosome-associated heterooligomeric protein complex required for mvb sorting." Babst M., Katzmann D.J., Estepa-Sabal E.J., Meerloo T., Emr S.D. Dev. Cell 3:271-282(2002) [PubMed: 12194857] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE ESCRT-III COMPLEX, INTERACTION WITH VPS20, SUBCELLULAR LOCATION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Protein-protein interactions of ESCRT complexes in the yeast Saccharomyces cerevisiae." Bowers K., Lottridge J., Helliwell S.B., Goldthwaite L.M., Luzio J.P., Stevens T.H. Traffic 5:194-210(2004) [PubMed: 15086794] [Abstract] Cited for: INTERACTION WITH BRO1.
[7]	"Structural basis for endosomal targeting by the Bro1 domain." Kim J., Sitaraman S., Hierro A., Beach B.M., Odorizzi G., Hurley J.H. Dev. Cell 8:937-947(2005) [PubMed: 15935782] [Abstract] Cited for: INTERACTION WITH BRO1.
[8]	"Vta1p and Vps46p regulate the membrane association and ATPase activity of Vps4p at the yeast multivesicular body." Lottridge J.M., Flannery A.R., Vincelli J.L., Stevens T.H. Proc. Natl. Acad. Sci. U.S.A. 103:6202-6207(2006) [PubMed: 16601096] [Abstract] Cited for: INTERACTION WITH VTA1.
[9]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-72, MASS SPECTROMETRY.
[10]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-72 AND SER-119, MASS SPECTROMETRY.
[11]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-213, MASS SPECTROMETRY.
[12]	"Ordered assembly of the ESCRT-III complex on endosomes is required to sequester cargo during MVB formation." Teis D., Saksena S., Emr S.D. Dev. Cell 15:578-589(2008) [PubMed: 18854142] [Abstract] Cited for: ASSEMBLY OF THE ESCRT-III COMPLEX.
[13]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-72; THR-116; SER-119; SER-193 AND SER-204, MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	L09751 Genomic DNA. No translation available. Z73197 Genomic DNA. Translation: CAA97548.1.
PIR	S52590.
RefSeq	NP_013125.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1747N.
IntAct	P39929. 18 interactions.
STRING	P39929.
Proteomic databases
PeptideAtlas	P39929.
Genome annotation databases
Ensembl	YLR025W; YLR025W; YLR025W; Saccharomyces cerevisiae. [Genome view]
GeneID	850712.
KEGG	sce:YLR025W.
NMPDR	fig\|4932.3.peg.4116.
Organism-specific databases
CYGD	YLR025w.
SGD	S000004015. SNF7.
Phylogenomic databases
HOGENOM	P39929.
OMA	PSAQEAI
OrthoDB	EOG96HHTF
Gene expression databases
ArrayExpress	P39929.
Genevestigator	P39929.
GermOnline	YLR025W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005024. Snf7. [Graphical view]
Pfam	PF03357. Snf7. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	966772.

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Entry information

Entry name

SNF7_YEAST

Accession

Primary (citable) accession number: P39929

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 24, 2009
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents