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Protein

Osmosensing histidine protein kinase SLN1

Gene

SLN1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Histidine kinase that acts as a osmosensor at the plasma membrane. Part of the bifurcated SLN1-YPD1-SKN7/SSK1 two-component regulatory system, which controls activity of the HOG1 pathway and gene expression in response to changes in the osmolarity of the extracellular environment. Under normal osmotic conditions, the histidine kinase autophosphorylates His-576. This phosphate is subsequently transferred to Asp-1144, from where it is relayed to 'His-64' of the phosphorelay intermediate protein YPD1. Under high osmolarity conditions, the histidine kinase is no longer active.2 Publications

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi1094 – 10941MagnesiumBy similarity
Metal bindingi1095 – 10951MagnesiumBy similarity
Metal bindingi1144 – 11441MagnesiumBy similarity
Metal bindingi1195 – 11951MagnesiumBy similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • histidine phosphotransfer kinase activity Source: SGD
  • metal ion binding Source: UniProtKB-KW
  • osmosensor activity Source: SGD
  • phosphorelay response regulator activity Source: GO_Central
  • phosphorelay sensor kinase activity Source: InterPro
  • protein histidine kinase activity Source: SGD

GO - Biological processi

  • negative regulation of protein kinase activity Source: SGD
  • osmosensory signaling via phosphorelay pathway Source: SGD
  • peptidyl-histidine phosphorylation Source: SGD
  • protein autophosphorylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Two-component regulatory system

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31396-MONOMER.
BRENDAi2.7.13.3. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Osmosensing histidine protein kinase SLN1 (EC:2.7.13.3)
Alternative name(s):
Osmolarity two-component system protein SLN1
Tyrosine phosphatase-dependent protein 2
Gene namesi
Name:SLN1
Synonyms:YPD2
Ordered Locus Names:YIL147C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIL147C.
SGDiS000001409. SLN1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2222CytoplasmicSequence analysisAdd
BLAST
Transmembranei23 – 4624HelicalSequence analysisAdd
BLAST
Topological domaini47 – 333287ExtracellularSequence analysisAdd
BLAST
Transmembranei334 – 35421HelicalSequence analysisAdd
BLAST
Topological domaini355 – 1220866CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: SGD
  • intracellular Source: GOC
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi576 – 5761H → Q: Inactive. 1 Publication
Mutagenesisi891 – 8911G → D in SLN1-1; slow growth.
Mutagenesisi1144 – 11441D → N: Inactive. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12201220Osmosensing histidine protein kinase SLN1PRO_0000081405Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi100 – 1001N-linked (GlcNAc...)Sequence analysis
Glycosylationi138 – 1381N-linked (GlcNAc...)Sequence analysis
Glycosylationi142 – 1421N-linked (GlcNAc...)Sequence analysis
Glycosylationi181 – 1811N-linked (GlcNAc...)Sequence analysis
Glycosylationi224 – 2241N-linked (GlcNAc...)Sequence analysis
Glycosylationi272 – 2721N-linked (GlcNAc...)Sequence analysis
Modified residuei502 – 5021PhosphoserineCombined sources
Modified residuei576 – 5761Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1 Publication
Modified residuei758 – 7581PhosphoserineCombined sources
Modified residuei833 – 8331PhosphoserineCombined sources
Modified residuei1041 – 10411PhosphoserineCombined sources
Modified residuei1044 – 10441PhosphoserineCombined sources
Modified residuei1144 – 114414-aspartylphosphatePROSITE-ProRule annotation1 Publication

Post-translational modificationi

The phosphorelay mechanism involves the sequential transfer of a phosphate group from His-576 (H1) in the histidine kinase domain (transmitter domain) to Asp-1144 (D1) of the response regulatory domain (receiver domain). This transfer probably occurs between two SLN1 molecules, rather than intramolecularly. The phosphate group is further transferred to 'His-64' (H2) of YPD1 and finally to 'Asp-554' (D2) of SSK1 or 'Asp-427' (D2) of SKN7.

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP39928.

PTM databases

iPTMnetiP39928.

Interactioni

Subunit structurei

Interacts with DJP1, MOG1 and YPD1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
YPD1Q076882EBI-17357,EBI-34423

Protein-protein interaction databases

BioGridi34845. 38 interactions.
DIPiDIP-2939N.
IntActiP39928. 29 interactions.
MINTiMINT-682970.

Structurei

Secondary structure

1
1220
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi1090 – 10934Combined sources
Helixi1097 – 110913Combined sources
Beta strandi1115 – 11206Combined sources
Helixi1121 – 113414Combined sources
Beta strandi1139 – 11435Combined sources
Beta strandi1148 – 11503Combined sources
Helixi1152 – 116211Combined sources
Beta strandi1169 – 11746Combined sources
Helixi1178 – 11869Combined sources
Beta strandi1190 – 11967Combined sources
Helixi1199 – 120911Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OXBX-ray2.30B1087-1220[»]
1OXKX-ray2.10B/D/F/H/J/L1087-1220[»]
2R25X-ray1.70B1086-1218[»]
ProteinModelPortaliP39928.
SMRiP39928. Positions 471-717, 815-928, 1058-1214.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP39928.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini573 – 928356Histidine kinasePROSITE-ProRule annotationAdd
BLAST
Domaini1089 – 1210122Response regulatoryPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 histidine kinase domain.PROSITE-ProRule annotation
Contains 1 response regulatory domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000248613.
InParanoidiP39928.
KOiK11231.
OMAiFPLAVWF.
OrthoDBiEOG092C0AJ1.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 2 hits.
InterProiIPR011006. CheY-like_superfamily.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF55874. SSF55874. 2 hits.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39928-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFGLPSKLE LTPPFRIGIR TQLTALVSIV ALGSLIILAV TTGVYFTSNY
60 70 80 90 100
KNLRSDRLYI AAQLKSSQID QTLNYLYYQA YYLASRDALQ SSLTSYVAGN
110 120 130 140 150
KSADNWVDSL SVIQKFLSSS NLFYVAKVYD SSFNAVLNAT NNGTGDLIPE
160 170 180 190 200
DVLDSLFPLS TDTPLPSSLE TIGILTDPVL NSTDYLMSMS LPIFANPSII
210 220 230 240 250
LTDSRVYGYI TIIMSAEGLK SVFNDTTALE HSTIAIISAV YNSQGKASGY
260 270 280 290 300
HFVFPPYGSR SDLPQKVFSI KNDTFISSAF RNGKGGSLKQ TNILSTRNTA
310 320 330 340 350
LGYSPCSFNL VNWVAIVSQP ESVFLSPATK LAKIITGTVI AIGVFVILLT
360 370 380 390 400
LPLAHWAVQP IVRLQKATEL ITEGRGLRPS TPRTISRASS FKRGFSSGFA
410 420 430 440 450
VPSSLLQFNT AEAGSTTSVS GHGGSGHGSG AAFSANSSMK SAINLGNEKM
460 470 480 490 500
SPPEEENKIP NNHTDAKISM DGSLNHDLLG PHSLRHNDTD RSSNRSHILT
510 520 530 540 550
TSANLTEARL PDYRRLFSDE LSDLTETFNT MTDALDQHYA LLEERVRART
560 570 580 590 600
KQLEAAKIEA EAANEAKTVF IANISHELRT PLNGILGMTA ISMEETDVNK
610 620 630 640 650
IRNSLKLIFR SGELLLHILT ELLTFSKNVL QRTKLEKRDF CITDVALQIK
660 670 680 690 700
SIFGKVAKDQ RVRLSISLFP NLIRTMVLWG DSNRIIQIVM NLVSNALKFT
710 720 730 740 750
PVDGTVDVRM KLLGEYDKEL SEKKQYKEVY IKKGTEVTEN LETTDKYDLP
760 770 780 790 800
TLSNHRKSVD LESSATSLGS NRDTSTIQEE ITKRNTVANE SIYKKVNDRE
810 820 830 840 850
KASNDDVSSI VSTTTSSYDN AIFNSQFNKA PGSDDEEGGN LGRPIENPKT
860 870 880 890 900
WVISIEVEDT GPGIDPSLQE SVFHPFVQGD QTLSRQYGGT GLGLSICRQL
910 920 930 940 950
ANMMHGTMKL ESKVGVGSKF TFTLPLNQTK EISFADMEFP FEDEFNPESR
960 970 980 990 1000
KNRRVKFSVA KSIKSRQSTS SVATPATNRS SLTNDVLPEV RSKGKHETKD
1010 1020 1030 1040 1050
VGNPNMGREE KNDNGGLEQL QEKNIKPSIC LTGAEVNEQN SLSSKHRSRH
1060 1070 1080 1090 1100
EGLGSVNLDR PFLQSTGTAT SSRNIPTVKD DDKNETSVKI LVVEDNHVNQ
1110 1120 1130 1140 1150
EVIKRMLNLE GIENIELACD GQEAFDKVKE LTSKGENYNM IFMDVQMPKV
1160 1170 1180 1190 1200
DGLLSTKMIR RDLGYTSPIV ALTAFADDSN IKECLESGMN GFLSKPIKRP
1210 1220
KLKTILTEFC AAYQGKKNNK
Length:1,220
Mass (Da):134,435
Last modified:February 1, 1995 - v1
Checksum:i45FFE24A8165486B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01835 Unassigned DNA. Translation: AAC48912.1.
Z38059 Genomic DNA. Translation: CAA86131.1.
BK006942 Genomic DNA. Translation: DAA08406.1.
PIRiS48387.
RefSeqiNP_012119.1. NM_001179495.1.

Genome annotation databases

EnsemblFungiiYIL147C; YIL147C; YIL147C.
GeneIDi854659.
KEGGisce:YIL147C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01835 Unassigned DNA. Translation: AAC48912.1.
Z38059 Genomic DNA. Translation: CAA86131.1.
BK006942 Genomic DNA. Translation: DAA08406.1.
PIRiS48387.
RefSeqiNP_012119.1. NM_001179495.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OXBX-ray2.30B1087-1220[»]
1OXKX-ray2.10B/D/F/H/J/L1087-1220[»]
2R25X-ray1.70B1086-1218[»]
ProteinModelPortaliP39928.
SMRiP39928. Positions 471-717, 815-928, 1058-1214.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34845. 38 interactions.
DIPiDIP-2939N.
IntActiP39928. 29 interactions.
MINTiMINT-682970.

PTM databases

iPTMnetiP39928.

Proteomic databases

MaxQBiP39928.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYIL147C; YIL147C; YIL147C.
GeneIDi854659.
KEGGisce:YIL147C.

Organism-specific databases

EuPathDBiFungiDB:YIL147C.
SGDiS000001409. SLN1.

Phylogenomic databases

HOGENOMiHOG000248613.
InParanoidiP39928.
KOiK11231.
OMAiFPLAVWF.
OrthoDBiEOG092C0AJ1.

Enzyme and pathway databases

BioCyciYEAST:G3O-31396-MONOMER.
BRENDAi2.7.13.3. 984.

Miscellaneous databases

EvolutionaryTraceiP39928.
PROiP39928.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 2 hits.
InterProiIPR011006. CheY-like_superfamily.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF55874. SSF55874. 2 hits.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSLN1_YEAST
AccessioniPrimary (citable) accession number: P39928
Secondary accession number(s): D6VVE0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 7, 2016
This is version 163 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 656 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.