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P39927

- PTI1_YEAST

UniProt

P39927 - PTI1_YEAST

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Protein

Protein PTI1

Gene

PTI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. Component of the APT complex, which may be involved in polyadenylation-independent transcript 3'-end formation. PTI1 is required for 3'-end formation of snoRNAs.1 Publication

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. nucleotide binding Source: InterPro

GO - Biological processi

  1. mRNA cleavage Source: SGD
  2. mRNA polyadenylation Source: SGD
  3. snoRNA 3'-end processing Source: SGD
  4. termination of RNA polymerase II transcription, exosome-dependent Source: SGD
  5. termination of RNA polymerase II transcription, poly(A)-coupled Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-30857-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein PTI1
Gene namesi
Name:PTI1
Ordered Locus Names:YGR156W
ORF Names:G6670
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGR156w.
SGDiS000003388. PTI1.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. cytosol Source: SGD
  2. mRNA cleavage and polyadenylation specificity factor complex Source: SGD
  3. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 425425Protein PTI1PRO_0000076294Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei272 – 2721Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP39927.
PaxDbiP39927.

Expressioni

Gene expression databases

GenevestigatoriP39927.

Interactioni

Subunit structurei

Component of the cleavage and polyadenylation factor (CPF) complex, which is composed of PTI1, SYC1, SSU72, GLC7, MPE1, REF2, PFS2, PTA1, YSH1/BRR5, SWD2, CFT2/YDH1, YTH1, CFT1/YHH1, FIP1 and PAP1. Component of the APT complex, which is a subcomplex of CPF, and is composed of PTI1, SYC1, SSU72, GLC7, REF2, PTA1 and SWD2.1 Publication

Protein-protein interaction databases

BioGridi33404. 47 interactions.
DIPiDIP-4402N.
IntActiP39927. 15 interactions.
MINTiMINT-562054.
STRINGi4932.YGR156W.

Structurei

3D structure databases

ProteinModelPortaliP39927.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiNOG148794.
HOGENOMiHOG000000936.
InParanoidiP39927.
KOiK14407.
OMAiLLMGFID.
OrthoDBiEOG7N63XZ.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR025742. CSTF2_hinge.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF14327. CSTF2_hinge. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39927-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTDPRRRTGR HFLTPENLSS TLQITNLPPE WNQDIITSVV AGSGPVIDIK
60 70 80 90 100
AKNDPRTGKL TGVLFDYLTS KDCKRAWEIL NRIENFPVKI EQIIPPNYKD
110 120 130 140 150
HLRETANKNS QKQVLQLNRD SYPFEAGLEL PFEMVTEVPI PRRPPPPQAA
160 170 180 190 200
NNTNSVSNNT NIQFPDILSK ASKHLPSFQD GSIIAPDKIS QNLSKIPPLQ
210 220 230 240 250
LIEIISNLKI LSNQENIQKS QLESFLDTNS DITISVTQAL LEMGFIDYSV
260 270 280 290 300
VTKVLKSQVG EAPSLLSSNN TSNSNTPVSV IRNNTPLHVP SNEVSNNPNN
310 320 330 340 350
MPLNVAMPMP MSTPPFIPLP LQQQPFGFAP PGPFMPPAQG PSMGQPVLAN
360 370 380 390 400
QLGQVQQQNI SSTEGPSNAN KANDSGTINM AKLQLLPENQ QDMIKQVLTL
410 420
TPAQIQSLPS DQQLMVENFR KEYII
Length:425
Mass (Da):46,983
Last modified:February 1, 1996 - v2
Checksum:iF6564311A2B1F240
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti300 – 3001N → Y in BAA03953. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D16502 Genomic DNA. Translation: BAA03953.1.
X85807 Genomic DNA. Translation: CAA59813.1.
Z72941 Genomic DNA. Translation: CAA97170.1.
AY557841 Genomic DNA. Translation: AAS56167.1.
BK006941 Genomic DNA. Translation: DAA08247.1.
PIRiS60446.
RefSeqiNP_011672.1. NM_001181285.1.

Genome annotation databases

EnsemblFungiiYGR156W; YGR156W; YGR156W.
GeneIDi853060.
KEGGisce:YGR156W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D16502 Genomic DNA. Translation: BAA03953.1 .
X85807 Genomic DNA. Translation: CAA59813.1 .
Z72941 Genomic DNA. Translation: CAA97170.1 .
AY557841 Genomic DNA. Translation: AAS56167.1 .
BK006941 Genomic DNA. Translation: DAA08247.1 .
PIRi S60446.
RefSeqi NP_011672.1. NM_001181285.1.

3D structure databases

ProteinModelPortali P39927.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33404. 47 interactions.
DIPi DIP-4402N.
IntActi P39927. 15 interactions.
MINTi MINT-562054.
STRINGi 4932.YGR156W.

Proteomic databases

MaxQBi P39927.
PaxDbi P39927.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGR156W ; YGR156W ; YGR156W .
GeneIDi 853060.
KEGGi sce:YGR156W.

Organism-specific databases

CYGDi YGR156w.
SGDi S000003388. PTI1.

Phylogenomic databases

eggNOGi NOG148794.
HOGENOMi HOG000000936.
InParanoidi P39927.
KOi K14407.
OMAi LLMGFID.
OrthoDBi EOG7N63XZ.

Enzyme and pathway databases

BioCyci YEAST:G3O-30857-MONOMER.

Miscellaneous databases

NextBioi 972995.

Gene expression databases

Genevestigatori P39927.

Family and domain databases

Gene3Di 3.30.70.330. 1 hit.
InterProi IPR025742. CSTF2_hinge.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view ]
Pfami PF14327. CSTF2_hinge. 1 hit.
[Graphical view ]
SMARTi SM00360. RRM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Ono B., Inoue T., Kijima K., Matsuda A., Negishi K., Shinoda S.
    Submitted (JUN-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: A5-8-1A.
  2. "The sequence of a 27 kb segment on the right arm of chromosome VII from Saccharomyces cerevisiae reveals MOL1, NAT2, RPL30B, RSR1, CYS4, PEM1/CHO2, NSR1 genes and ten new open reading frames."
    Skala J., Nawrocki A., Goffeau A.
    Yeast 11:1421-1427(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "Pti1p and Ref2p found in association with the mRNA 3' end formation complex direct snoRNA maturation."
    Dheur S., Vo le T.A., Voisinet-Hakil F., Minet M., Schmitter J.-M., Lacroute F., Wyers F., Minvielle-Sebastia L.
    EMBO J. 22:2831-2840(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN SNORNA 3-END FORMATION.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends."
    Nedea E., He X., Kim M., Pootoolal J., Zhong G., Canadien V., Hughes T., Buratowski S., Moore C.L., Greenblatt J.
    J. Biol. Chem. 278:33000-33010(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CPF COMPLEX, COMPOSITION OF THE APT COMPLEX, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
  9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-272, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPTI1_YEAST
AccessioniPrimary (citable) accession number: P39927
Secondary accession number(s): D6VUT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 937 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3