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Protein

Protein PTI1

Gene

PTI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. Component of the APT complex, which may be involved in polyadenylation-independent transcript 3'-end formation. PTI1 is required for 3'-end formation of snoRNAs.1 Publication

Miscellaneous

Present with 937 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

GO - Biological processi

  • mRNA polyadenylation Source: SGD
  • pre-mRNA cleavage required for polyadenylation Source: SGD
  • snoRNA 3'-end processing Source: SGD
  • termination of RNA polymerase II transcription, exosome-dependent Source: SGD
  • termination of RNA polymerase II transcription, poly(A)-coupled Source: SGD

Keywordsi

Biological processmRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-30857-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Protein PTI1
Gene namesi
Name:PTI1
Ordered Locus Names:YGR156W
ORF Names:G6670
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR156W
SGDiS000003388 PTI1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000762941 – 425Protein PTI1Add BLAST425

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei272PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP39927
PaxDbiP39927
PRIDEiP39927

PTM databases

iPTMnetiP39927

Interactioni

Subunit structurei

Component of the cleavage and polyadenylation factor (CPF) complex, which is composed of PTI1, SYC1, SSU72, GLC7, MPE1, REF2, PFS2, PTA1, YSH1/BRR5, SWD2, CFT2/YDH1, YTH1, CFT1/YHH1, FIP1 and PAP1. Component of the APT complex, which is a subcomplex of CPF, and is composed of PTI1, SYC1, SSU72, GLC7, REF2, PTA1 and SWD2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PTA1Q013293EBI-23382,EBI-14145

Protein-protein interaction databases

BioGridi33404, 534 interactors
DIPiDIP-4402N
IntActiP39927, 16 interactors
MINTiP39927
STRINGi4932.YGR156W

Structurei

3D structure databases

ProteinModelPortaliP39927
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

HOGENOMiHOG000000936
InParanoidiP39927
KOiK14407
OMAiSKIPPAQ
OrthoDBiEOG092C3MCG

Family and domain databases

Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR025742 CSTF2_hinge
IPR026896 CSTF_C
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PfamiView protein in Pfam
PF14327 CSTF2_hinge, 1 hit
PF14304 CSTF_C, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit

Sequencei

Sequence statusi: Complete.

P39927-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDPRRRTGR HFLTPENLSS TLQITNLPPE WNQDIITSVV AGSGPVIDIK
60 70 80 90 100
AKNDPRTGKL TGVLFDYLTS KDCKRAWEIL NRIENFPVKI EQIIPPNYKD
110 120 130 140 150
HLRETANKNS QKQVLQLNRD SYPFEAGLEL PFEMVTEVPI PRRPPPPQAA
160 170 180 190 200
NNTNSVSNNT NIQFPDILSK ASKHLPSFQD GSIIAPDKIS QNLSKIPPLQ
210 220 230 240 250
LIEIISNLKI LSNQENIQKS QLESFLDTNS DITISVTQAL LEMGFIDYSV
260 270 280 290 300
VTKVLKSQVG EAPSLLSSNN TSNSNTPVSV IRNNTPLHVP SNEVSNNPNN
310 320 330 340 350
MPLNVAMPMP MSTPPFIPLP LQQQPFGFAP PGPFMPPAQG PSMGQPVLAN
360 370 380 390 400
QLGQVQQQNI SSTEGPSNAN KANDSGTINM AKLQLLPENQ QDMIKQVLTL
410 420
TPAQIQSLPS DQQLMVENFR KEYII
Length:425
Mass (Da):46,983
Last modified:February 1, 1996 - v2
Checksum:iF6564311A2B1F240
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti300N → Y in BAA03953 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16502 Genomic DNA Translation: BAA03953.1
X85807 Genomic DNA Translation: CAA59813.1
Z72941 Genomic DNA Translation: CAA97170.1
AY557841 Genomic DNA Translation: AAS56167.1
BK006941 Genomic DNA Translation: DAA08247.1
PIRiS60446
RefSeqiNP_011672.1, NM_001181285.1

Genome annotation databases

EnsemblFungiiYGR156W; YGR156W; YGR156W
GeneIDi853060
KEGGisce:YGR156W

Similar proteinsi

Entry informationi

Entry nameiPTI1_YEAST
AccessioniPrimary (citable) accession number: P39927
Secondary accession number(s): D6VUT6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1996
Last modified: May 23, 2018
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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