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P39927

- PTI1_YEAST

UniProt

P39927 - PTI1_YEAST

Protein

Protein PTI1

Gene

PTI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 2 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. Component of the APT complex, which may be involved in polyadenylation-independent transcript 3'-end formation. PTI1 is required for 3'-end formation of snoRNAs.1 Publication

    GO - Molecular functioni

    1. nucleic acid binding Source: InterPro
    2. nucleotide binding Source: InterPro

    GO - Biological processi

    1. mRNA cleavage Source: SGD
    2. mRNA polyadenylation Source: SGD
    3. snoRNA 3'-end processing Source: SGD
    4. termination of RNA polymerase II transcription, exosome-dependent Source: SGD
    5. termination of RNA polymerase II transcription, poly(A)-coupled Source: SGD

    Keywords - Biological processi

    mRNA processing

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30857-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein PTI1
    Gene namesi
    Name:PTI1
    Ordered Locus Names:YGR156W
    ORF Names:G6670
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGR156w.
    SGDiS000003388. PTI1.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. cytosol Source: SGD
    2. mRNA cleavage and polyadenylation specificity factor complex Source: SGD
    3. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 425425Protein PTI1PRO_0000076294Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei272 – 2721Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP39927.
    PaxDbiP39927.

    Expressioni

    Gene expression databases

    GenevestigatoriP39927.

    Interactioni

    Subunit structurei

    Component of the cleavage and polyadenylation factor (CPF) complex, which is composed of PTI1, SYC1, SSU72, GLC7, MPE1, REF2, PFS2, PTA1, YSH1/BRR5, SWD2, CFT2/YDH1, YTH1, CFT1/YHH1, FIP1 and PAP1. Component of the APT complex, which is a subcomplex of CPF, and is composed of PTI1, SYC1, SSU72, GLC7, REF2, PTA1 and SWD2.1 Publication

    Protein-protein interaction databases

    BioGridi33404. 46 interactions.
    DIPiDIP-4402N.
    IntActiP39927. 15 interactions.
    MINTiMINT-562054.
    STRINGi4932.YGR156W.

    Structurei

    3D structure databases

    ProteinModelPortaliP39927.
    SMRiP39927. Positions 19-95, 384-424.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    eggNOGiNOG148794.
    HOGENOMiHOG000000936.
    KOiK14407.
    OMAiLLMGFID.
    OrthoDBiEOG7N63XZ.

    Family and domain databases

    Gene3Di3.30.70.330. 1 hit.
    InterProiIPR025742. CSTF2_hinge.
    IPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    [Graphical view]
    PfamiPF14327. CSTF2_hinge. 1 hit.
    [Graphical view]
    SMARTiSM00360. RRM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P39927-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTDPRRRTGR HFLTPENLSS TLQITNLPPE WNQDIITSVV AGSGPVIDIK    50
    AKNDPRTGKL TGVLFDYLTS KDCKRAWEIL NRIENFPVKI EQIIPPNYKD 100
    HLRETANKNS QKQVLQLNRD SYPFEAGLEL PFEMVTEVPI PRRPPPPQAA 150
    NNTNSVSNNT NIQFPDILSK ASKHLPSFQD GSIIAPDKIS QNLSKIPPLQ 200
    LIEIISNLKI LSNQENIQKS QLESFLDTNS DITISVTQAL LEMGFIDYSV 250
    VTKVLKSQVG EAPSLLSSNN TSNSNTPVSV IRNNTPLHVP SNEVSNNPNN 300
    MPLNVAMPMP MSTPPFIPLP LQQQPFGFAP PGPFMPPAQG PSMGQPVLAN 350
    QLGQVQQQNI SSTEGPSNAN KANDSGTINM AKLQLLPENQ QDMIKQVLTL 400
    TPAQIQSLPS DQQLMVENFR KEYII 425
    Length:425
    Mass (Da):46,983
    Last modified:February 1, 1996 - v2
    Checksum:iF6564311A2B1F240
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti300 – 3001N → Y in BAA03953. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D16502 Genomic DNA. Translation: BAA03953.1.
    X85807 Genomic DNA. Translation: CAA59813.1.
    Z72941 Genomic DNA. Translation: CAA97170.1.
    AY557841 Genomic DNA. Translation: AAS56167.1.
    BK006941 Genomic DNA. Translation: DAA08247.1.
    PIRiS60446.
    RefSeqiNP_011672.1. NM_001181285.1.

    Genome annotation databases

    EnsemblFungiiYGR156W; YGR156W; YGR156W.
    GeneIDi853060.
    KEGGisce:YGR156W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D16502 Genomic DNA. Translation: BAA03953.1 .
    X85807 Genomic DNA. Translation: CAA59813.1 .
    Z72941 Genomic DNA. Translation: CAA97170.1 .
    AY557841 Genomic DNA. Translation: AAS56167.1 .
    BK006941 Genomic DNA. Translation: DAA08247.1 .
    PIRi S60446.
    RefSeqi NP_011672.1. NM_001181285.1.

    3D structure databases

    ProteinModelPortali P39927.
    SMRi P39927. Positions 19-95, 384-424.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33404. 46 interactions.
    DIPi DIP-4402N.
    IntActi P39927. 15 interactions.
    MINTi MINT-562054.
    STRINGi 4932.YGR156W.

    Proteomic databases

    MaxQBi P39927.
    PaxDbi P39927.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR156W ; YGR156W ; YGR156W .
    GeneIDi 853060.
    KEGGi sce:YGR156W.

    Organism-specific databases

    CYGDi YGR156w.
    SGDi S000003388. PTI1.

    Phylogenomic databases

    eggNOGi NOG148794.
    HOGENOMi HOG000000936.
    KOi K14407.
    OMAi LLMGFID.
    OrthoDBi EOG7N63XZ.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30857-MONOMER.

    Miscellaneous databases

    NextBioi 972995.

    Gene expression databases

    Genevestigatori P39927.

    Family and domain databases

    Gene3Di 3.30.70.330. 1 hit.
    InterProi IPR025742. CSTF2_hinge.
    IPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    [Graphical view ]
    Pfami PF14327. CSTF2_hinge. 1 hit.
    [Graphical view ]
    SMARTi SM00360. RRM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Ono B., Inoue T., Kijima K., Matsuda A., Negishi K., Shinoda S.
      Submitted (JUN-1993) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: A5-8-1A.
    2. "The sequence of a 27 kb segment on the right arm of chromosome VII from Saccharomyces cerevisiae reveals MOL1, NAT2, RPL30B, RSR1, CYS4, PEM1/CHO2, NSR1 genes and ten new open reading frames."
      Skala J., Nawrocki A., Goffeau A.
      Yeast 11:1421-1427(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "Pti1p and Ref2p found in association with the mRNA 3' end formation complex direct snoRNA maturation."
      Dheur S., Vo le T.A., Voisinet-Hakil F., Minet M., Schmitter J.-M., Lacroute F., Wyers F., Minvielle-Sebastia L.
      EMBO J. 22:2831-2840(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN SNORNA 3-END FORMATION.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends."
      Nedea E., He X., Kim M., Pootoolal J., Zhong G., Canadien V., Hughes T., Buratowski S., Moore C.L., Greenblatt J.
      J. Biol. Chem. 278:33000-33010(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE CPF COMPLEX, COMPOSITION OF THE APT COMPLEX, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
    9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-272, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPTI1_YEAST
    AccessioniPrimary (citable) accession number: P39927
    Secondary accession number(s): D6VUT6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 122 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 937 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3