P39926 (SSO2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein SSO2

Gene names

Name:	SSO2
Ordered Locus Names:	YMR183C
ORF Names:	YM8010.13C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for vesicle fusion with the plasma membrane.
Subcellular location	Membrane; Single-pass type IV membrane protein Potential.
Miscellaneous	Present with 1720 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the syntaxin family. Contains 1 t-SNARE coiled-coil homology domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Coiled coil Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	Golgi to plasma membrane transport Traceable author statement. Source: SGD ascospore-type prospore assembly Inferred from genetic interaction. Source: SGD intracellular protein transport Inferred from electronic annotation. Source: InterPro vesicle fusion Traceable author statement. Source: SGD
Cellular component	endoplasmic reticulum Inferred from direct assay. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay. Source: SGD plasma membrane enriched fraction Inferred from direct assay. Source: SGD prospore membrane Inferred from direct assay. Source: SGD
Molecular function	SNAP receptor activity Inferred from physical interaction. Source: SGD phosphatidic acid binding Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 295	295	Protein SSO2		PRO_0000210275
Regions
	Topological domain	1 – 269	269	Cytoplasmic Potential
	Transmembrane	270 – 291	22	Helical; Anchor for type IV membrane protein; Potential
	Topological domain	292 – 295	4	Extracellular Potential
	Domain	194 – 256	63	t-SNARE coiled-coil homology
	Coiled coil	39 – 100	62	Potential
Amino acid modifications
	Modified residue	31	1	Phosphoserine Ref.5
	Modified residue	34	1	Phosphoserine Ref.5
Experimental info
	Sequence conflict	222	1	E → K in CAA47960. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P39926 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 454CF2B45FFE8E22	FASTA	295	33,734
	10 20 30 40 50 60 MSNANPYENN NPYAENYEMQ EDLNNAPTGH SDGSDDFVAF MNKINSINAN LSRYENIINQ 70 80 90 100 110 120 IDAQHKDLLT QVSEEQEMEL RRSLDDYISQ ATDLQYQLKA DIKDAQRDGL HDSNKQAQAE 130 140 150 160 170 180 NCRQKFLKLI QDYRIIDSNY KEESKEQAKR QYTIIQPEAT DEEVEAAIND VNGQQIFSQA 190 200 210 220 230 240 LLNANRRGEA KTALAEVQAR HQELLKLEKT MAELTQLFND MEELVIEQQE NVDVIDKNVE 250 260 270 280 290 DAQQDVEQGV GHTNKAVKSA RKARKNKIRC LIICFIIFAI VVVVVVVPSV VETRK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Yeast syntaxins Sso1p and Sso2p belong to a family of related membrane proteins that function in vesicular transport." Aalto M.K., Ronne H., Keraenen S. EMBO J. 12:4095-4104(1993) [PubMed: 8223426] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ATCC 26787 / X2180-1B.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-31 AND SER-34, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X67730 mRNA. Translation: CAA47960.1. Z49808 Genomic DNA. Translation: CAA89916.1. BK006946 Genomic DNA. Translation: DAA10081.1.
PIR	S55130.
RefSeq	NP_013908.1. NM_001182689.1.
3D structure databases
ProteinModelPortal	P39926.
SMR	P39926. Positions 36-261.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2951N.
IntAct	P39926. 3 interactions.
STRING	P39926.
Proteomic databases
PeptideAtlas	P39926.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR183C; YMR183C; YMR183C.
GeneID	855221.
KEGG	sce:YMR183C.
NMPDR	fig|4932.3.peg.4960.
Organism-specific databases
CYGD	YMR183c.
SGD	S000004795. SSO2.
Phylogenomic databases
eggNOG	fuNOG08328.
GeneTree	EFGT00050000002424.
HOGENOM	HBG735129.
OMA	DGDIESG.
OrthoDB	EOG4CNV12.
Gene expression databases
ArrayExpress	P39926.
Genevestigator	P39926.
GermOnline	YMR183C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006012. Syntaxin/epimorphin_CS. IPR006011. Syntaxin_N. IPR010989. t-SNARE. IPR000727. T_SNARE_dom. [Graphical view]
KO	K08486.
Pfam	PF05739. SNARE. 1 hit. [Graphical view]
SMART	SM00503. SynN. 1 hit. SM00397. t_SNARE. 1 hit. [Graphical view]
SUPFAM	SSF47661. t-snare. 1 hit.
PROSITE	PS00914. SYNTAXIN. 1 hit. PS50192. T_SNARE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	978741.

Entry information

Entry name

SSO2_YEAST

Accession

Primary (citable) accession number: P39926
Secondary accession number(s): D6W007

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 100 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program