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Protein

Mitochondrial respiratory chain complexes assembly protein AFG3

Gene

AFG3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a component of the m-AAA protease complex which is a ATP-dependent metalloprotease mediating degradation of non-assembled mitochondrial inner membrane proteins. The complex is necessary for the assembly of mitochondrial respiratory chain and ATPase complexes. Function both in post-translational assembly and in the turnover of mistranslated or misfolded polypeptides.2 Publications

Cofactori

Zn2+CuratedNote: Binds 1 zinc ion per subunit.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi558Zinc; catalyticBy similarity1
Active sitei559By similarity1
Metal bindingi562Zinc; catalyticBy similarity1
Metal bindingi634Zinc; catalyticBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi328 – 335ATPSequence analysis8

GO - Molecular functioni

  • ATPase activity Source: SGD
  • ATP binding Source: UniProtKB-KW
  • ATP-dependent peptidase activity Source: GO_Central
  • metalloendopeptidase activity Source: InterPro
  • metallopeptidase activity Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • cristae formation Source: GO_Central
  • cytoplasmic translation Source: SGD
  • mitochondrial fusion Source: GO_Central
  • mitochondrial protein processing Source: GO_Central
  • protein complex assembly Source: SGD
  • protein import into mitochondrial intermembrane space Source: GO_Central
  • protein import into mitochondrial matrix Source: SGD
  • proteolysis Source: SGD
  • replicative cell aging Source: SGD
  • signal peptide processing Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-30202-MONOMER.

Protein family/group databases

MEROPSiM41.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial respiratory chain complexes assembly protein AFG3 (EC:3.4.24.-)
Alternative name(s):
ATPase family gene 3 protein
Tat-binding homolog 10
Gene namesi
Name:AFG3
Synonyms:YTA10
Ordered Locus Names:YER017C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER017C.
SGDiS000000819. AFG3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei116 – 136HelicalSequence analysisAdd BLAST21
Transmembranei224 – 244HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • m-AAA complex Source: SGD
  • mitochondrial inner boundary membrane Source: SGD
  • mitochondrial inner membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi559E → Q: Abolishes proteolytic activity; impairs synthesis of respiratory chain proteins COB and COX1. No effect on m-AAA protease assembly. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000846701 – 761Mitochondrial respiratory chain complexes assembly protein AFG3Add BLAST761

Proteomic databases

MaxQBiP39925.
PRIDEiP39925.

Interactioni

Subunit structurei

Component of the 850 kDa m-AAA protease complex (YTA10-12) which consists of multiple copies of RCA1 AND AFG3.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
YTA12P403413EBI-2317,EBI-14858

Protein-protein interaction databases

BioGridi36750. 80 interactors.
DIPiDIP-802N.
IntActiP39925. 7 interactors.
MINTiMINT-563722.

Structurei

3D structure databases

ProteinModelPortaliP39925.
SMRiP39925.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

In the N-terminal section; belongs to the AAA ATPase family.Curated
In the C-terminal section; belongs to the peptidase M41 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00860000133808.
HOGENOMiHOG000217277.
InParanoidiP39925.
KOiK08956.
OMAiIVFGKIT.
OrthoDBiEOG092C147V.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_01458. FtsH. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR005936. FtsH.
IPR027417. P-loop_NTPase.
IPR011546. Pept_M41_FtsH_extracell.
IPR000642. Peptidase_M41.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF06480. FtsH_ext. 1 hit.
PF01434. Peptidase_M41. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01241. FtsH_fam. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39925-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMMWQRYARG APRSLTSLSF GKASRISTVK PVLRSRMPVH QRLQTLSGLA
60 70 80 90 100
TRNTIHRSTQ IRSFHISWTR LNENRPNKEG EGKNNGNKDN NSNKEDGKDK
110 120 130 140 150
RNEFGSLSEY FRSKEFANTM FLTIGFTIIF TLLTPSSNNS GDDSNRVLTF
160 170 180 190 200
QDFKTKYLEK GLVSKIYVVN KFLVEAELVN TKQVVSFTIG SVDIFEEQMD
210 220 230 240 250
QIQDLLNIPP RDRIPIKYIE RSSPFTFLFP FLPTIILLGG LYFITRKINS
260 270 280 290 300
SPPNANGGGG GGLGGMFNVG KSRAKLFNKE TDIKISFKNV AGCDEAKQEI
310 320 330 340 350
MEFVHFLKNP GKYTKLGAKI PRGAILSGPP GTGKTLLAKA TAGEANVPFL
360 370 380 390 400
SVSGSEFVEM FVGVGASRVR DLFTQARSMA PSIIFIDEID AIGKERGKGG
410 420 430 440 450
ALGGANDERE ATLNQLLVEM DGFTTSDQVV VLAGTNRPDV LDNALMRPGR
460 470 480 490 500
FDRHIQIDSP DVNGRQQIYL VHLKRLNLDP LLTDDMNNLS GKLATLTPGF
510 520 530 540 550
TGADIANACN EAALIAARHN DPYITIHHFE QAIERVIAGL EKKTRVLSKE
560 570 580 590 600
EKRSVAYHEA GHAVCGWFLK YADPLLKVSI IPRGQGALGY AQYLPPDQYL
610 620 630 640 650
ISEEQFRHRM IMALGGRVSE ELHFPSVTSG AHDDFKKVTQ MANAMVTSLG
660 670 680 690 700
MSPKIGYLSF DQNDGNFKVN KPFSNKTART IDLEVKSIVD DAHRACTELL
710 720 730 740 750
TKNLDKVDLV AKELLRKEAI TREDMIRLLG PRPFKERNEA FEKYLDPKSN
760
TEPPEAPAAT N
Length:761
Mass (Da):84,544
Last modified:February 1, 1995 - v1
Checksum:i517C1F0E81ABE841
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti411A → R in CAA54091 (PubMed:7754704).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X81066 Genomic DNA. Translation: CAA56953.1.
X76643 Genomic DNA. Translation: CAA54091.1.
U18778 Genomic DNA. Translation: AAB64550.1.
BK006939 Genomic DNA. Translation: DAA07669.1.
PIRiS46611.
RefSeqiNP_010933.1. NM_001178908.1.

Genome annotation databases

EnsemblFungiiYER017C; YER017C; YER017C.
GeneIDi856737.
KEGGisce:YER017C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X81066 Genomic DNA. Translation: CAA56953.1.
X76643 Genomic DNA. Translation: CAA54091.1.
U18778 Genomic DNA. Translation: AAB64550.1.
BK006939 Genomic DNA. Translation: DAA07669.1.
PIRiS46611.
RefSeqiNP_010933.1. NM_001178908.1.

3D structure databases

ProteinModelPortaliP39925.
SMRiP39925.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36750. 80 interactors.
DIPiDIP-802N.
IntActiP39925. 7 interactors.
MINTiMINT-563722.

Protein family/group databases

MEROPSiM41.002.

Proteomic databases

MaxQBiP39925.
PRIDEiP39925.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER017C; YER017C; YER017C.
GeneIDi856737.
KEGGisce:YER017C.

Organism-specific databases

EuPathDBiFungiDB:YER017C.
SGDiS000000819. AFG3.

Phylogenomic databases

GeneTreeiENSGT00860000133808.
HOGENOMiHOG000217277.
InParanoidiP39925.
KOiK08956.
OMAiIVFGKIT.
OrthoDBiEOG092C147V.

Enzyme and pathway databases

BioCyciYEAST:G3O-30202-MONOMER.

Miscellaneous databases

PROiP39925.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_01458. FtsH. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR005936. FtsH.
IPR027417. P-loop_NTPase.
IPR011546. Pept_M41_FtsH_extracell.
IPR000642. Peptidase_M41.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF06480. FtsH_ext. 1 hit.
PF01434. Peptidase_M41. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01241. FtsH_fam. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAFG3_YEAST
AccessioniPrimary (citable) accession number: P39925
Secondary accession number(s): D3DLR5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 30, 2016
This is version 165 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3870 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.