P39925 (AFG3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial respiratory chain complexes assembly protein AFG3
EC=3.4.24.-

Alternative name(s):
ATPase family gene 3 protein
Tat-binding homolog 10

Gene names

Name:	AFG3
Synonyms:	YTA10
Ordered Locus Names:	YER017C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	761 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a component of the m-AAA protease complex which is a ATP-dependent metalloprotease mediating degradation of non-assembled mitochondrial inner membrane proteins. The complex is necessary for the assembly of mitochondrial respiratory chain and ATPase complexes. Function both in post-translational assembly and in the turnover of mistranslated or misfolded polypeptides. Ref.5 Ref.6
Cofactor	Binds 1 zinc ion per subunit Potential.
Subunit structure	Component of the 850 kDa m-AAA protease complex (YTA10-12) which consists of multiple copies of RCA1 AND AFG3.
Subcellular location	Mitochondrion membrane; Multi-pass membrane protein Ref.5.
Miscellaneous	Present with 3870 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	In the N-terminal section; belongs to the AAA ATPase family. In the C-terminal section; belongs to the peptidase M41 family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein catabolic process Inferred from electronic annotation. Source: InterPro protein complex assembly Inferred from mutant phenotype Ref.5. Source: SGD protein import into mitochondrial intermembrane space Traceable author statement. Source: SGD proteolysis Inferred from mutant phenotype Ref.5. Source: SGD signal peptide processing Inferred from mutant phenotype. Source: SGD
Cellular component	m-AAA complex Inferred from direct assay Ref.5. Source: SGD mitochondrial inner boundary membrane Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from direct assay. Source: SGD ATPase activity Inferred from mutant phenotype. Source: SGD metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
YTA12	P40341	3	EBI-2317,EBI-14858

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 761	761	Mitochondrial respiratory chain complexes assembly protein AFG3		PRO_0000084670
Regions
	Transmembrane	116 – 136	21	Helical; Potential
	Transmembrane	224 – 244	21	Helical; Potential
	Nucleotide binding	328 – 335	8	ATP Potential
Sites
	Active site	559	1	By similarity
	Metal binding	558	1	Zinc; catalytic By similarity
	Metal binding	562	1	Zinc; catalytic By similarity
	Metal binding	634	1	Zinc; catalytic By similarity
Experimental info
	Mutagenesis	559	1	E → Q: Abolishes proteolytic activity; impairs synthesis of respiratory chain proteins COB and COX1. No effect on m-AAA protease assembly. Ref.6
	Sequence conflict	411	1	A → R in CAA54091. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P39925 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 517C1F0E81ABE841	FASTA	761	84,544
	10 20 30 40 50 60 MMMWQRYARG APRSLTSLSF GKASRISTVK PVLRSRMPVH QRLQTLSGLA TRNTIHRSTQ 70 80 90 100 110 120 IRSFHISWTR LNENRPNKEG EGKNNGNKDN NSNKEDGKDK RNEFGSLSEY FRSKEFANTM 130 140 150 160 170 180 FLTIGFTIIF TLLTPSSNNS GDDSNRVLTF QDFKTKYLEK GLVSKIYVVN KFLVEAELVN 190 200 210 220 230 240 TKQVVSFTIG SVDIFEEQMD QIQDLLNIPP RDRIPIKYIE RSSPFTFLFP FLPTIILLGG 250 260 270 280 290 300 LYFITRKINS SPPNANGGGG GGLGGMFNVG KSRAKLFNKE TDIKISFKNV AGCDEAKQEI 310 320 330 340 350 360 MEFVHFLKNP GKYTKLGAKI PRGAILSGPP GTGKTLLAKA TAGEANVPFL SVSGSEFVEM 370 380 390 400 410 420 FVGVGASRVR DLFTQARSMA PSIIFIDEID AIGKERGKGG ALGGANDERE ATLNQLLVEM 430 440 450 460 470 480 DGFTTSDQVV VLAGTNRPDV LDNALMRPGR FDRHIQIDSP DVNGRQQIYL VHLKRLNLDP 490 500 510 520 530 540 LLTDDMNNLS GKLATLTPGF TGADIANACN EAALIAARHN DPYITIHHFE QAIERVIAGL 550 560 570 580 590 600 EKKTRVLSKE EKRSVAYHEA GHAVCGWFLK YADPLLKVSI IPRGQGALGY AQYLPPDQYL 610 620 630 640 650 660 ISEEQFRHRM IMALGGRVSE ELHFPSVTSG AHDDFKKVTQ MANAMVTSLG MSPKIGYLSF 670 680 690 700 710 720 DQNDGNFKVN KPFSNKTART IDLEVKSIVD DAHRACTELL TKNLDKVDLV AKELLRKEAI 730 740 750 760 TREDMIRLLG PRPFKERNEA FEKYLDPKSN TEPPEAPAAT N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a set of yeast genes coding for a novel family of putative ATPases with high similarity to constituents of the 26S protease complex." Schnall R., Mannhaupt G., Stucka R., Tauer R., Ehnle S., Schwarzlose C., Vetter I., Feldmann H. Yeast 10:1141-1155(1994) [PubMed: 7754704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Sequence of the AFG3 gene encoding a new member of the FtsH/Yme1/Tma subfamily of the AAA-protein family." Guelin E.J.M., Rep M., Grivell L.A. Yeast 10:1389-1394(1994) [PubMed: 7900428] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / YPH1.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"The YTA10-12 complex, an AAA protease with chaperone-like activity in the inner membrane of mitochondria." Arlt H., Tauer R., Feldmann H., Neupert W., Langer T. Cell 85:875-885(1996) [PubMed: 8681382] [Abstract] Cited for: IDENTIFICATION IN THE M-AAA PROTEASE COMPLEX, FUNCTION OF THE M-AAA PROTEASE COMPLEX, SUBCELLULAR LOCATION.
[6]	"The formation of respiratory chain complexes in mitochondria is under the proteolytic control of the m-AAA protease." Arlt H., Steglich G., Perryman R., Guiard B., Neupert W., Langer T. EMBO J. 17:4837-4847(1998) [PubMed: 9707443] [Abstract] Cited for: FUNCTION OF THE M-AAA PROTEASE COMPLEX, MUTAGENESIS OF GLU-559.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X81066 Genomic DNA. Translation: CAA56953.1. X76643 Genomic DNA. Translation: CAA54091.1. U18778 Genomic DNA. Translation: AAB64550.1. BK006939 Genomic DNA. Translation: DAA07669.1.
PIR	S46611.
RefSeq	NP_010933.1. NM_001178908.1.
3D structure databases
ProteinModelPortal	P39925.
SMR	P39925. Positions 280-731.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-802N.
IntAct	P39925. 8 interactions.
MINT	MINT-563722.
STRING	P39925.
Protein family/group databases
MEROPS	M41.002.
Proteomic databases
PeptideAtlas	P39925.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YER017C; YER017C; YER017C.
GeneID	856737.
KEGG	sce:YER017C.
NMPDR	fig|4932.3.peg.1993.
Organism-specific databases
CYGD	YER017c.
SGD	S000000819. AFG3.
Phylogenomic databases
eggNOG	fuNOG04410.
GeneTree	EFGT00050000000010.
HOGENOM	HBG724153.
OMA	QIPVLYK.
OrthoDB	EOG4J14HG.
Gene expression databases
ArrayExpress	P39925.
Genevestigator	P39925.
GermOnline	YER017C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR003960. ATPase_AAA_CS. IPR005936. Pept_M41_FtsH. IPR011546. Pept_M41_FtsH_extracell. IPR000642. Peptidase_M41. [Graphical view]
KO	K08956.
Pfam	PF00004. AAA. 1 hit. PF06480. FtsH_ext. 1 hit. PF01434. Peptidase_M41. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01241. FtsH_fam. 1 hit.
PROSITE	PS00674. AAA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982861.

Entry information

Entry name

AFG3_YEAST

Accession

Primary (citable) accession number: P39925
Secondary accession number(s): D3DLR5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	January 25, 2012
This is version 119 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program