Reviewed, UniProtKB/Swiss-Prot P39923 (NPR2_YEAST)

Names and origin

Protein names

Recommended name:
    Nitrogen permease regulator 2

Gene names

Name:	NPR2
Ordered Locus Names:	YEL062W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	615 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Post-transcriptional regulator of nitrogen permeases. It could be involved in the putative NPR1-dependent phosphorylation of nitrogen permeases or in the processing and targeting of the nitrogen permeases at the level of the endoplasmic reticulum. Involved in cell kill provoked by cisplatin and doxorubicin.
Induction	By urea and proline.
Miscellaneous	Present with 319 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the NPR2 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular response to amino acid starvation Inferred from mutant phenotype. Source: SGD proline transport Ref.1 Inferred from mutant phenotype. Source: SGD regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW response to drug Ref.4 Inferred from mutant phenotype. Source: SGD transcription Inferred from electronic annotation. Source: UniProtKB-KW urea transport Ref.1 Inferred from mutant phenotype. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BBP1	Q12365	1	EBI-12212,EBI-3448

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 615	615	Nitrogen permease regulator 2		PRO_0000213321
Regions
	Compositional bias	354 – 420	67	Ser-rich
Amino acid modifications
	Modified residue	362	1	Phosphoserine Ref.7
Experimental info
	Sequence conflict	225	1	D → G in AAT92885. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P39923-1 [UniParc]. Last modified December 20, 2005. Version 2. Checksum: 58CA4983B0BAF7BA	FASTA	615	69,859
	10 20 30 40 50 60 MLSYFQGFVP IHTIFYSVFH PTEGSKIKYE FPPNNLKNHG INFNTFKNYI IPKPILCHKL 70 80 90 100 110 120 ITFKYGTYRI VCYPVTINSP IYARNFFSFN FVFVFPYDCE TSPYEPAITR LGKMFKVLEE 130 140 150 160 170 180 QNQLLSKSER DPVFFDLKVL ENSTTTPSTA GPSSTPNPSS NTTPTHPTSE KDTKDMRSSR 190 200 210 220 230 240 YSDLIKDLGL PQSAFSIQDL LMRIFQDLNN YSECLIPIDE GNAVDIKIFP LLRPPTTCVS 250 260 270 280 290 300 LEDVPLSSVN LKKIIDVNWD PTMMSIVPYI DGLNSIAKIS KLSNSDPGLV IECIRHLIYY 310 320 330 340 350 360 KCVTLSDIFQ FSNIYAPSSL IRNFLTDPLM ASDCQSYVTF PEVSKISNLP LNKSLGSGDQ 370 380 390 400 410 420 DSPSFSVRRK SKSSSIPSNP DSRTTSFSST SRVSQNSSLN SSFSSIYKDW RQSQTSCSSS 430 440 450 460 470 480 NIHVINNRNR FLPTRSCLFD LYRSLSQGQT LKTWYESKYM ILKENNIDIR RFITFGLEKR 490 500 510 520 530 540 IIYRCYSFPV MINAGSREPK EMTPIITKDL VNNDKLLEKR NHNHLLSATG SRNTAQSGNL 550 560 570 580 590 600 KPERPSKVSF EMQRVSSLAT GKSTMPKLSD EEEGILEESI RNAETFDKIC VLLSKPKLEV 610 ESYLNELGEF KVINS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A second nitrogen permease regulator in Saccharomyces cerevisiae." Rousselet G., Simon M., Ripoche P., Buhler J.-M. FEBS Lett. 359:215-219(1995) [PubMed: 7867803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Anticancer drug resistance induced by disruption of the Saccharomyces cerevisiae NPR2 gene: a novel component involved in cisplatin- and doxorubicin-provoked cell kill." Schenk P.W., Brok M., Boersma A.W., Brandsma J.A., Den Dulk H., Burger H., Stoter G., Brouwer J., Nooter K. Mol. Pharmacol. 64:259-268(2003) [PubMed: 12869630] [Abstract] Cited for: INVOLVEMENT IN CISPLATIN RESISTANCE.
[5]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed: 12748633] [Abstract] Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-362, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X79105 Genomic DNA. Translation: CAA55721.1. Different initiation. U18795 Genomic DNA. Translation: AAB65025.1. Different initiation. AY692866 Genomic DNA. Translation: AAT92885.1. Different initiation.
PIR	S44938.
RefSeq	NP_010852.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1985N.
IntAct	P39923. 3 interactions.
STRING	P39923.
Proteomic databases
PRIDE	P39923.
Genome annotation databases
Ensembl	YEL062W; YEL062W; YEL062W; Saccharomyces cerevisiae. [Genome view]
GeneID	856647.
KEGG	sce:YEL062W.
NMPDR	fig|4932.3.peg.1900.
Organism-specific databases
CYGD	YEL062w.
SGD	S000000788. NPR2.
Phylogenomic databases
HOGENOM	P39923.
OMA	QFSNIYA
OrthoDB	EOG9V9W78
Gene expression databases
ArrayExpress	P39923.
Genevestigator	P39923.
GermOnline	YEL062W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR009348. NPR2. [Graphical view]
Pfam	PF06218. NPR2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	982619.

Entry information

Entry name

NPR2_YEAST

Accession

Primary (citable) accession number: P39923
Secondary accession number(s): Q6B264

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	December 20, 2005
Last modified:	November 24, 2009
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)