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P39920 (FTSK_COXBU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA translocase FtsK
Gene names
Name:ftsK
Synonyms:spoIIIE
Ordered Locus Names:CBU_1191
OrganismCoxiella burnetii (strain RSA 493 / Nine Mile phase I) [Reference proteome] [HAMAP]
Taxonomic identifier227377 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella

Protein attributes

Sequence length778 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Essential cell division protein that coordinates cell division and chromosome segregation. The N-terminus is involved in assembly of the cell-division machinery. The C-terminus functions as a DNA motor that moves dsDNA in an ATP-dependent manner towards the dif recombination site, which is located within the replication terminus region. Translocation stops specifically at Xer-dif sites, where FtsK interacts with the Xer recombinase, allowing activation of chromosome unlinking by recombination. FtsK orienting polar sequences (KOPS) guide the direction of DNA translocation. FtsK can remove proteins from DNA as it translocates, but translocation stops specifically at XerCD-dif site, thereby preventing removal of XerC and XerD from dif By similarity.

Subunit structure

Homohexamer. Forms a ring that surrounds DNA By similarity.

Subcellular location

Cell inner membrane; Multi-pass membrane protein By similarity. Note: Located at the septum By similarity.

Domain

Consists of an N-terminal domain, which is sufficient for the localization to the septal ring and is required for cell division, followed by a linker domain, and a C-terminal domain, which forms the translocation motor involved in chromosome segregation. The C-terminal domain can be further subdivided into alpha, beta and gamma subdomains. The alpha and beta subdomains multimerise to produce a hexameric ring, contain the nucleotide binding motif and form the DNA pump. The gamma subdomain is a regulatory subdomain that controls translocation of DNA by recognition of KOPS motifs and interacts with XerD recombinase By similarity.

Sequence similarities

Belongs to the FtsK/SpoIIIE/SftA family.

Contains 1 FtsK domain.

Sequence caution

The sequence AAO90700.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 778778DNA translocase FtsK
PRO_0000098256

Regions

Transmembrane28 – 4821Helical; Potential
Transmembrane81 – 10121Helical; Potential
Transmembrane122 – 14221Helical; Potential
Transmembrane146 – 16621Helical; Potential
Transmembrane172 – 19221Helical; Potential
Topological domain193 – 778586Cytoplasmic Potential
Domain419 – 629211FtsK
Nucleotide binding439 – 4446ATP By similarity

Experimental info

Sequence conflict641A → P in CAA53289. Ref.1
Sequence conflict79 – 802LY → KN in CAA53289. Ref.1
Sequence conflict871Y → N in CAA53289. Ref.1
Sequence conflict1021R → P in CAA53289. Ref.1
Sequence conflict1211A → P in CAA53289. Ref.1
Sequence conflict1591A → P in CAA53289. Ref.1
Sequence conflict1681T → A in CAA53289. Ref.1
Sequence conflict1921F → C in CAA53289. Ref.1
Sequence conflict2001K → R in CAA53289. Ref.1
Sequence conflict2361T → A in CAA53289. Ref.1
Sequence conflict2451P → R in CAA53289. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P39920 [UniParc].

Last modified May 9, 2003. Version 2.
Checksum: 54B9D88081D73F7D

FASTA77885,251
        10         20         30         40         50         60 
MGRRKGKKSA KETGVLLRRH LRYRLREGCF ILALAFSAFL FIALLSYHRS DPGWSHSIVV 

        70         80         90        100        110        120 
KHVANLTGEA GAWLSDFTLY MVGYLAYIFP LMVAFAAWVF FRNRHEEQDI PTKWPLLILR 

       130        140        150        160        170        180 
AVGFLLILLA GSALAAIHLG TLDANLPYNG GGIIGVVMAK SLFPIFNTAG TSLILIAFLL 

       190        200        210        220        230        240 
IGITLFTGLS WFQFLELLGK NAIKFTKFCA IRLGAISWKD LFLSLLPSQD KREAVTVPKI 

       250        260        270        280        290        300 
KRVEPDLVPD ALDMISTPKI AERPKLEIID HEFKTPRFKG SAILPELSLL DKPSQDHTLS 

       310        320        330        340        350        360 
YSEEELQQKS REVELRLADF GIQAKVVAVH PGPVVTRFEL QLAAGTKASR VTNLAKDLAR 

       370        380        390        400        410        420 
SLSVISVRIV EVIPGKSVIG LELPNKNREV VTIYEVLATK QYQNARSSLT LALGKDIGGH 

       430        440        450        460        470        480 
PVIVDLAKMP HLLVAGTTGS GKSVSLNAML LSLLYKSTPQ QLRLILIDPK MLELSVYEGI 

       490        500        510        520        530        540 
PHLLTPVVTD MKDAAAALRW CVVEMERRYR LMASLGVRNI LGYNAKVKEA IEAGAPLLDP 

       550        560        570        580        590        600 
LQAAAEGKPP ELQELPQLVV IADEFADMMV VVGKKVETLI VRLAQKARAA GIHLIFATQR 

       610        620        630        640        650        660 
PSVDVITGLI KANIPTRVAF QVSSKIDSRT ILDQQGAEQL LGHGDLLYLA PGSGVPVRVH 

       670        680        690        700        710        720 
GPYVKDEEVH RVAEYLRESS EPNYVEGILD EMGAQDLSGF VEAALGGGSE EGGESDPLYD 

       730        740        750        760        770 
EAVEAVIRSR RVSVSSIQRR FKIGYNRAAR IVEAMEAAGV VSPMENNGAR EVLAPSKE 

« Hide

References

« Hide 'large scale' references
[1]"A sporulation gene in Coxiella burnetii?"
Oswald W., Thiele D.
J. Vet. Med. B 40:366-370(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Nine Mile phase I / Bratislava.
[2]"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii."
Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. expand/collapse author list , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F.
Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: RSA 493 / Nine Mile phase I.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X75627 Genomic DNA. Translation: CAA53289.1.
AE016828 Genomic DNA. Translation: AAO90700.2. Different initiation.
PIRS43132.
RefSeqNP_820186.2. NC_002971.3.

3D structure databases

ProteinModelPortalP39920.
SMRP39920. Positions 284-689, 711-776.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING227377.CBU_1191.

Proteomic databases

PRIDEP39920.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO90700; AAO90700; CBU_1191.
GeneID1209095.
KEGGcbu:CBU_1191.
PATRIC17931127. VBICoxBur82552_1189.

Phylogenomic databases

eggNOGCOG1674.
HOGENOMHOG000010003.
KOK03466.
OMAGFMSVFD.
OrthoDBEOG6S52GD.

Enzyme and pathway databases

BioCycCBUR227377:GJ7S-1180-MONOMER.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR003593. AAA+_ATPase.
IPR002543. Cell_div_FtsK/SpoIIIE.
IPR018541. DNA_translocase_Ftsk_gamma.
IPR025199. DUF4117.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF13491. DUF4117. 1 hit.
PF09397. Ftsk_gamma. 1 hit.
PF01580. FtsK_SpoIIIE. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 1 hit.
SM00843. Ftsk_gamma. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS50901. FTSK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFTSK_COXBU
AccessionPrimary (citable) accession number: P39920
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: May 9, 2003
Last modified: May 14, 2014
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Coxiella burnetii

Coxiella burnetii (strain RSA 493): entries and gene names