P39912 (AROG_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein AroA(G)

Including the following 2 domains:

1. Phospho-2-dehydro-3-deoxyheptonate aldolase
   EC=2.5.1.54
   Alternative name(s):
   3-deoxy-D-arabino-heptulosonate 7-phosphate synthase
   DAHP synthase
   Phospho-2-keto-3-deoxyheptonate aldolase
2. Chorismate mutase
   EC=5.4.99.5

Gene names

Name:	aroA
Ordered Locus Names:	BSU29750

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate. Chorismate = prephenate.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7. Metabolic intermediate biosynthesis; prephenate biosynthesis; prephenate from chorismate: step 1/1.
Sequence similarities	Belongs to the class-I DAHP synthase family. Contains 1 chorismate mutase domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Molecular function	Isomerase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-deoxy-7-phosphoheptulonate synthase activity Inferred from electronic annotation. Source: EC aldehyde-lyase activity Inferred from electronic annotation. Source: InterPro chorismate mutase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 358	358	Protein AroA(G)		PRO_0000140827
Regions
	Domain	1 – 90	90	Chorismate mutase
Amino acid modifications
	Modified residue	2	1	Phosphoserine Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P39912 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 95D217477A2DE5AC	FASTA	358	39,539
	10 20 30 40 50 60 MSNTELELLR QKADELNLQI LKLINERGNV VKEIGKAKEA QGVNRFDPVR ERTMLNNIIE 70 80 90 100 110 120 NNDGPFENST IQHIFKEIFK AGLELQEEDH SKALLVSRKK KPEDTIVDIK GEKIGDGQQR 130 140 150 160 170 180 FIVGPCAVES YEQVAEVAAA AKKQGIKILR GGAFKPRTSP YDFQGLGVEG LQILKRVADE 190 200 210 220 230 240 FDLAVISEIV TPAHIEEALD YIDVIQIGAR NMQNFELLKA AGAVKKPVLL KRGLAATISE 250 260 270 280 290 300 FINAAEYIMS QGNDQIILCE RGIRTYETAT RNTLDISAVP ILKQETHLPV FVDVTHSTGR 310 320 330 340 350 RDLLLPTAKA ALAIGADGVM AEVHPDPSVA LSDSAQQMAI PEFEKWLNEL KPMVKVNA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identical amino acid sequence of the aroA(G) gene products of Bacillus subtilis 168 and B. subtilis Marburg strain." Bolotin A.P., Khazak V.E., Stoynova N., Ratmanova K., Yomantas Y., Kozlov Y. Microbiology 141:2219-2222(1995) [PubMed: 7496534] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region." Lapidus A., Galleron N., Sorokin A., Ehrlich S.D. Microbiology 143:3431-3441(1997) [PubMed: 9387221] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis." Macek B., Mijakovic I., Olsen J.V., Gnad F., Kumar C., Jensen P.R., Mann M. Mol. Cell. Proteomics 6:697-707(2007) [PubMed: 17218307] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2, MASS SPECTROMETRY. Strain: 168.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X65945 Genomic DNA. Translation: CAA46760.1. AF008220 Genomic DNA. Translation: AAC00298.1. AL009126 Genomic DNA. Translation: CAB14953.1.
PIR	S21418.
RefSeq	NP_390853.1. NC_000964.3.
3D structure databases
ProteinModelPortal	P39912.
SMR	P39912. Positions 9-350.
ModBase	Search...
PTM databases
PhosSite	P39912.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000003496; EBBACP00000003496; EBBACG00000003489.
GeneID	937853.
GenomeReviews	Gene locus BSU29750 in contig AL009126_GR.
KEGG	bsu:BSU29750.
NMPDR	fig|224308.1.peg.2978.
PATRIC	18977858. VBIBacSub10457_3117.
Organism-specific databases
GenoList	BSU29750. [Micado]
Phylogenomic databases
GeneTree	EBGT00050000002473.
HOGENOM	HBG575990.
OMA	STIQHIF.
PhylomeDB	P39912.
ProtClustDB	PRK12595.
Enzyme and pathway databases
BioCyc	BSUB:BSU29750-MONOMER.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR002701. Chorismate_mutase. IPR010954. Chorismate_mutase_GmP-bac. IPR020822. Chorismate_mutase_type_II. IPR006218. DAHP1/KDSA. IPR006268. DAHP_syn_2. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit. G3DSA:1.20.59.10. Chorismate_mutase. 1 hit.
KO	K13853.
Pfam	PF01817. CM_2. 1 hit. PF00793. DAHP_synth_1. 1 hit. [Graphical view]
SMART	SM00830. CM_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR01801. CM_A. 1 hit. TIGR01361. DAHP_synth_Bsub. 1 hit.
PROSITE	PS51168. CHORISMATE_MUT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROG_BACSU

Accession

Primary (citable) accession number: P39912

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	January 25, 2012
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program