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P39890

- TCMI_STRGA

UniProt

P39890 - TCMI_STRGA

Protein

Tetracenomycin F2 cyclase

Gene

tcmI

Organism
Streptomyces glaucescens
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Catalyzing the conversion of tetracenomycin F2 to tetracenomycin F1.1 Publication

    Catalytic activityi

    Tetracenomycin F2 = tetracenomycin F1 + H2O.1 Publication

    Kineticsi

    1. KM=121 µM for tetracenomycin F21 Publication

    Vmax=704 nmol/min/mg enzyme1 Publication

    pH dependencei

    Optimum pH is 6-6.5.1 Publication

    Pathwayi

    GO - Biological processi

    1. antibiotic biosynthetic process Source: UniProtKB-KW
    2. polyketide biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Antibiotic biosynthesis

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-18608.
    UniPathwayiUPA00174.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tetracenomycin F2 cyclase (EC:4.2.1.154)
    Alternative name(s):
    Tetracenomycin polyketide synthesis protein TcmI
    Gene namesi
    Name:tcmI
    OrganismiStreptomyces glaucescens
    Taxonomic identifieri1907 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi26 – 261H → A: Relative activity reduced to 15% of wild-type. 1 Publication
    Mutagenesisi26 – 261H → Q: No effect. 1 Publication
    Mutagenesisi27 – 271D → N: Relative activity reduced to 14% of wild-type. 1 Publication
    Mutagenesisi40 – 401R → G: Relative activity reduced to 10% of wild-type. 1 Publication
    Mutagenesisi40 – 401R → K: Relative activity reduced to 16% of wild-type. 1 Publication
    Mutagenesisi51 – 511H → A: Relative activity reduced to 16% of wild-type. 1 Publication
    Mutagenesisi51 – 511H → Q: No effect. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 109108Tetracenomycin F2 cyclasePRO_0000072454Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Structurei

    Secondary structure

    1
    109
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 119
    Helixi13 – 153
    Helixi16 – 2611
    Helixi31 – 355
    Beta strandi39 – 457
    Beta strandi48 – 5710
    Helixi60 – 656
    Helixi70 – 723
    Helixi73 – 808
    Beta strandi83 – 875
    Helixi93 – 964

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1TUWX-ray1.90A1-109[»]
    ProteinModelPortaliP39890.
    SMRiP39890. Positions 2-107.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP39890.

    Family & Domainsi

    Family and domain databases

    Gene3Di3.30.70.1090. 1 hit.
    InterProiIPR011008. Dimeric_a/b-barrel.
    IPR006765. Polyketide_synth_cyclase.
    [Graphical view]
    PfamiPF04673. Cyclase_polyket. 1 hit.
    [Graphical view]
    ProDomiPD012644. Polyketide_synth_cyclase. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF54909. SSF54909. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P39890-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAYRALMVLR MDPADAEHVA AAFAEHDTTE LPLEIGVRRR VLFRFHDLYM    50
    HLIEADDDIM ERLYQARSHP LFQEVNERVG QYLTPYAQDW EELKDSKAEV 100
    FYSWTAPDS 109
    Length:109
    Mass (Da):12,861
    Last modified:February 1, 1995 - v1
    Checksum:i1F9E3EC9E1B90593
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M80674 Genomic DNA. Translation: AAA67513.1.
    PIRiB53291.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M80674 Genomic DNA. Translation: AAA67513.1 .
    PIRi B53291.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1TUW X-ray 1.90 A 1-109 [» ]
    ProteinModelPortali P39890.
    SMRi P39890. Positions 2-107.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00174 .
    BioCyci MetaCyc:MONOMER-18608.

    Miscellaneous databases

    EvolutionaryTracei P39890.

    Family and domain databases

    Gene3Di 3.30.70.1090. 1 hit.
    InterProi IPR011008. Dimeric_a/b-barrel.
    IPR006765. Polyketide_synth_cyclase.
    [Graphical view ]
    Pfami PF04673. Cyclase_polyket. 1 hit.
    [Graphical view ]
    ProDomi PD012644. Polyketide_synth_cyclase. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF54909. SSF54909. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and transcriptional analysis of the Streptomyces glaucescens tcmAR tetracenomycin C resistance and repressor gene loci."
      Guilfoile P.G., Hutchinson C.R.
      J. Bacteriol. 174:3651-3658(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: DSM 40716 / ETH 22794 / GLA.0.
    2. "Tetracenomycin F2 cyclase: intramolecular aldol condensation in the biosynthesis of tetracenomycin C in Streptomyces glaucescens."
      Shen B., Hutchinson C.R.
      Biochemistry 32:11149-11154(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-15, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: WMH1068.
    3. "Structural and functional analysis of tetracenomycin F2 cyclase from Streptomyces glaucescens. A type II polyketide cyclase."
      Thompson T.B., Katayama K., Watanabe K., Hutchinson C.R., Rayment I.
      J. Biol. Chem. 279:37956-37963(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS), SUBUNIT, MUTAGENESIS OF HIS-26; ASP-27; ARG-40 AND HIS-51.

    Entry informationi

    Entry nameiTCMI_STRGA
    AccessioniPrimary (citable) accession number: P39890
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3