P39882 (NIA_LOTTE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nitrate reductase [NADH]
Short name=NR
EC=1.7.1.1

Gene names

Name:

NIA

Organism

Lotus tetragonolobus (Winged pea) (Tetragonolobus purpureus)

Taxonomic identifier

3868 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Loteae › Lotus

Protein attributes

Sequence length	160 AA.
Sequence status	Fragment.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.
Catalytic activity	Nitrite + NAD+ + H2O = nitrate + NADH.
Cofactor	Binds 1 FAD. Binds 1 heme group. The heme group is called cytochrome b-557. Binds 1 molybdenum ion.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the nitrate reductase family. Contains 1 cytochrome b5 heme-binding domain.

Ontologies

Keywords
Biological process	Nitrate assimilation
Ligand	FAD Flavoprotein Heme Iron Metal-binding Molybdenum NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	nitrate assimilation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitrate reductase (NADH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 160	›160	Nitrate reductase [NADH]		PRO_0000166060
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P39882 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: D6CFC1447393A932	FASTA	160	17,728
	10 20 30 40 50 60 KGPLGHIEYT GKGNFIAHGE PKFARRLAML AGGTGITPIY QVIQAVLKDP DDRTEMFFAV 70 80 90 100 110 120 YSNKTESDIL LREELDAWAK KHSDRFKVWY VVDKAGNDWA FSTGRVNESI MRVHLPGPSD 130 140 150 160 ALALACGPPP INSAYGWQPS LENIGYKKDD VPVFMATTQS

References

[1]	"Molecular cloning and analysis winged bean and soybean bispecific constitutive NR and their NiR." Wu S., Lu Q., Kriz A.L., Harper J.E. Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Leaf.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L16780 mRNA. Translation: AAA80564.1.
3D structure databases
ProteinModelPortal	P39882.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001834. NADH-Cyt_B5_reductase. IPR001433. OxRdtase_FAD/NAD-bd. [Graphical view]
Pfam	PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00371. FPNCR.
PROSITE	PS00191. CYTOCHROME_B5_1. Partial match. PS50255. CYTOCHROME_B5_2. Partial match. PS00559. MOLYBDOPTERIN_EUK. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NIA_LOTTE

Accession

Primary (citable) accession number: P39882

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	September 21, 2011
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program