Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Exodeoxyribonuclease 1

Gene

EXO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

5'->3' double-stranded DNA exonuclease involved in mismatch repair and eventually also in mitotic recombination between direct repeats. Also has a minor role in the correction of large DNA mismatches that occur in the heteroduplex DNA during meiotic recombination at the HIS4 locus.4 Publications

Miscellaneous

Present with 672 molecules/cell in log phase SD medium.1 Publication

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.By similarity

Enzyme regulationi

Inactivated by calcium and zinc ions.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi30Magnesium 1By similarity1
Metal bindingi78Magnesium 1By similarity1
Metal bindingi150Magnesium 1By similarity1
Metal bindingi152Magnesium 1By similarity1
Metal bindingi171Magnesium 2By similarity1
Metal bindingi173Magnesium 2By similarity1
Metal bindingi227Magnesium 2By similarity1

GO - Molecular functioni

  • 5'-3' exodeoxyribonuclease activity Source: InterPro
  • 5'-3' exonuclease activity Source: SGD
  • 5'-flap endonuclease activity Source: SGD
  • DNA binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • DNA double-strand break processing Source: SGD
  • gene conversion at mating-type locus, DNA double-strand break processing Source: SGD
  • meiotic DNA double-strand break processing Source: SGD
  • mismatch repair Source: SGD
  • telomere maintenance Source: SGD
  • telomeric 3' overhang formation Source: SGD

Keywordsi

Molecular functionDNA-binding, Excision nuclease, Exonuclease, Hydrolase, Nuclease
Biological processDNA damage, DNA excision, DNA repair
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33579-MONOMER
BRENDAi3.1.11.1 984
ReactomeiR-SCE-5358565 Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha)

Names & Taxonomyi

Protein namesi
Recommended name:
Exodeoxyribonuclease 1 (EC:3.1.-.-)
Alternative name(s):
Exodeoxyribonuclease I
Short name:
EXO I
Short name:
Exonuclease I
Protein DHS1
Gene namesi
Name:EXO1
Synonyms:DHS1
Ordered Locus Names:YOR033C
ORF Names:OR26.23
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR033C
SGDiS000005559 EXO1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001540451 – 702Exodeoxyribonuclease 1Add BLAST702

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei372PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP39875
PaxDbiP39875
PRIDEiP39875

PTM databases

iPTMnetiP39875

Interactioni

Subunit structurei

Interacts with mismatch repair protein MSH2.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi34435, 187 interactors
DIPiDIP-2421N
IntActiP39875, 6 interactors
STRINGi4932.YOR033C

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4FMOX-ray3.04C443-450[»]
ProteinModelPortaliP39875
SMRiP39875
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 96N-domainAdd BLAST96
Regioni114 – 247I-domainAdd BLAST134

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi501 – 520Asp/Glu-rich (acidic)Add BLAST20
Compositional biasi537 – 553Asp/Glu-rich (acidic)Add BLAST17
Compositional biasi618 – 625Asp-rich (acidic)8

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00510000047676
HOGENOMiHOG000112416
InParanoidiP39875
KOiK10746
OMAiACSCAYE
OrthoDBiEOG092C2BJU

Family and domain databases

CDDicd09908 H3TH_EXO1, 1 hit
InterProiView protein in InterPro
IPR036279 5-3_exonuclease_C_sf
IPR032641 Exo1
IPR037315 EXO1_H3TH
IPR008918 HhH2
IPR029060 PIN-like_dom_sf
IPR006086 XPG-I_dom
IPR006084 XPG/Rad2
IPR019974 XPG_CS
IPR006085 XPG_DNA_repair_N
PANTHERiPTHR11081 PTHR11081, 1 hit
PTHR11081:SF8 PTHR11081:SF8, 1 hit
PfamiView protein in Pfam
PF00867 XPG_I, 1 hit
PF00752 XPG_N, 1 hit
PRINTSiPR00853 XPGRADSUPER
SMARTiView protein in SMART
SM00279 HhH2, 1 hit
SM00484 XPGI, 1 hit
SM00485 XPGN, 1 hit
SUPFAMiSSF47807 SSF47807, 1 hit
SSF88723 SSF88723, 1 hit
PROSITEiView protein in PROSITE
PS00841 XPG_1, 1 hit
PS00842 XPG_2, 1 hit

Sequencei

Sequence statusi: Complete.

P39875-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGIQGLLPQL KPIQNPVSLR RYEGEVLAID GYAWLHRAAC SCAYELAMGK
60 70 80 90 100
PTDKYLQFFI KRFSLLKTFK VEPYLVFDGD AIPVKKSTES KRRDKRKENK
110 120 130 140 150
AIAERLWACG EKKNAMDYFQ KCVDITPEMA KCIICYCKLN GIRYIVAPFE
160 170 180 190 200
ADSQMVYLEQ KNIVQGIISE DSDLLVFGCR RLITKLNDYG ECLEICRDNF
210 220 230 240 250
IKLPKKFPLG SLTNEEIITM VCLSGCDYTN GIPKVGLITA MKLVRRFNTI
260 270 280 290 300
ERIILSIQRE GKLMIPDTYI NEYEAAVLAF QFQRVFCPIR KKIVSLNEIP
310 320 330 340 350
LYLKDTESKR KRLYACIGFV IHRETQKKQI VHFDDDIDHH LHLKIAQGDL
360 370 380 390 400
NPYDFHQPLA NREHKLQLAS KSNIEFGKTN TTNSEAKVKP IESFFQKMTK
410 420 430 440 450
LDHNPKVANN IHSLRQAEDK LTMAIKRRKL SNANVVQETL KDTRSKFFNK
460 470 480 490 500
PSMTVVENFK EKGDSIQDFK EDTNSQSLEE PVSESQLSTQ IPSSFITTNL
510 520 530 540 550
EDDDNLSEEV SEVVSDIEED RKNSEGKTIG NEIYNTDDDG DGDTSEDYSE
560 570 580 590 600
TAESRVPTSS TTSFPGSSQR SISGCTKVLQ KFRYSSSFSG VNANRQPLFP
610 620 630 640 650
RHVNQKSRGM VYVNQNRDDD CDDNDGKNQI TQRPSLRKSL IGARSQRIVI
660 670 680 690 700
DMKSVDERKS FNSSPILHEE SKKRDIETTK SSQARPAVRS ISLLSQFVYK

GK
Length:702
Mass (Da):80,162
Last modified:October 1, 1996 - v2
Checksum:i78E22F6B265DB3AA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U86134 Genomic DNA Translation: AAB47428.1
X87331 Genomic DNA Translation: CAA60749.1
Z74941 Genomic DNA Translation: CAA99223.1
S69545 Genomic DNA Translation: AAC60570.1
BK006948 Genomic DNA Translation: DAA10815.1
PIRiS62169
RefSeqiNP_014676.1, NM_001183452.1

Genome annotation databases

EnsemblFungiiYOR033C; YOR033C; YOR033C
GeneIDi854198
KEGGisce:YOR033C

Similar proteinsi

Entry informationi

Entry nameiEXO1_YEAST
AccessioniPrimary (citable) accession number: P39875
Secondary accession number(s): D6W299
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 1, 1996
Last modified: June 20, 2018
This is version 165 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health