Reviewed, UniProtKB/Swiss-Prot P39871 (NIA2_MAIZE)

Names and origin

Protein names

Recommended name:
    Nitrate reductase [NAD(P)H]
      Short name=NR
    EC=1.7.1.2

Gene names

Name:

NAR

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACCAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	231 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.
Catalytic activity	Nitrite + NAD(P)+ + H2O = nitrate + NAD(P)H.
Cofactor	Binds 1 FAD. Binds 1 heme group. The heme group is called cytochrome b-557. Binds 1 molybdenum ion.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the nitrate reductase family. Contains 1 cytochrome b5 heme-binding domain. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Biological process	Nitrate assimilation
Ligand	FAD Flavoprotein Heme Iron Metal-binding Molybdenum NAD NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	nitrate assimilation Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitrate reductase [NAD(P)H] activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 231	›231	Nitrate reductase [NAD(P)H]		PRO_0000166063
Regions
	Domain	‹1 – 85	›85	FAD-binding FR-type
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P39871-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 9864B425C0ED45F4	FASTA	231	26,254
	10 20 30 40 50 60 PQKLGLPVGR HVYVCASIGG KLCMRAYTPT SPVDEVGHFD LLIKIYFKDE DPKYPNGGLM 70 80 90 100 110 120 SQYLDSLPLG ATIDIKGPHR HIEYTGRRRF VVNGKQRHAR RLAMIQAGRG TTPDDDTEQA 130 140 150 160 170 180 VLRDQPDDDT EMHLVYANRT DHDMLLREEI DRAWLPRTRR LKVWYVVSKV PEDGWEYGVG 190 200 210 220 230 RVDEHVMREH LPLGDSETIA LVCGPPAMIE CTVRPGLEKM GYDLDKACLV F

References

[1]	"Regulation of maize root nitrate reductase mRNA levels." Long D.M., Oaks A., Rothstein S.J. Physiol. Plantarum 85:561-566(1992) Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. W64A X WI82E. Tissue: Root.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X64446 mRNA. Translation: CAA45776.1.
PIR	S24544.
UniGene	Zm.42640
3D structure databases
HSSP	HSSP built from PDB template 2CND based on UniProtKB P17571.
ModBase	Search...
Organism-specific databases
Gramene	P39871.
MaizeGDB	25891.
Enzyme and pathway databases
BRENDA	1.7.1.2. 289.
Family and domain databases
InterPro	IPR018506. Cyt_B5_heme-BS. IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd. IPR001433. OxRdtase_FAD/NAD_bd. IPR000572. OxRdtase_Mopterin-bd. [Graphical view]
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00371. FPNCR.
PROSITE	PS00191. CYTOCHROME_B5_1. Partial match. PS50255. CYTOCHROME_B5_2. Partial match. PS51384. FAD_FR. 1 hit. PS00559. MOLYBDOPTERIN_EUK. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NIA2_MAIZE

Accession

Primary (citable) accession number: P39871

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 5, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)