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Protein

Inducible nitrate reductase [NADH] 2

Gene

INR2

Organism
Glycine max (Soybean) (Glycine hispida)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.

Catalytic activityi

Nitrite + NAD+ + H2O = nitrate + NADH.

Cofactori

Protein has several cofactor binding sites:
  • FADBy similarityNote: Binds 1 FAD.By similarity
  • hemeBy similarityNote: Binds 1 heme group. The heme group is called cytochrome b-557.By similarity
  • Mo-molybdopterinBy similarityNote: Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi169 – 1691MolybdenumBy similarity
Metal bindingi552 – 5521Iron (heme axial ligand)PROSITE-ProRule annotation
Metal bindingi575 – 5751Iron (heme axial ligand)PROSITE-ProRule annotation

GO - Molecular functioni

  1. electron carrier activity Source: InterPro
  2. flavin adenine dinucleotide binding Source: InterPro
  3. heme binding Source: InterPro
  4. iron ion binding Source: InterPro
  5. molybdenum ion binding Source: InterPro
  6. molybdopterin cofactor binding Source: InterPro
  7. nitrate reductase (NADH) activity Source: UniProtKB-EC

GO - Biological processi

  1. nitrate assimilation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Nitrate assimilation

Keywords - Ligandi

FAD, Flavoprotein, Heme, Iron, Metal-binding, Molybdenum, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Inducible nitrate reductase [NADH] 2 (EC:1.7.1.1)
Short name:
NR
Gene namesi
Name:INR2
OrganismiGlycine max (Soybean) (Glycine hispida)
Taxonomic identifieri3847 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja
ProteomesiUP000008827: Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 890890Inducible nitrate reductase [NADH] 2PRO_0000166070Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi408 – 408InterchainSequence Analysis

Keywords - PTMi

Disulfide bond

Expressioni

Gene expression databases

GenevestigatoriP39870.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP39870.
SMRiP39870. Positions 636-890.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini517 – 59276Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini634 – 746113FAD-binding FR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the nitrate reductase family.Curated
Contains 1 cytochrome b5 heme-binding domain.PROSITE-ProRule annotation
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation

Phylogenomic databases

InParanoidiP39870.
KOiK10534.

Family and domain databases

Gene3Di2.60.40.650. 1 hit.
3.10.120.10. 1 hit.
3.90.420.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR014756. Ig_E-set.
IPR005066. MoCF_OxRdtse_dimer.
IPR008335. Mopterin_OxRdtase_euk.
IPR001834. NADH-Cyt_B5_reductase.
IPR012137. Nitr_rd_NADH.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR000572. OxRdtase_Mopterin-bd_dom.
IPR022407. OxRdtase_Mopterin_BS.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
PF00970. FAD_binding_6. 1 hit.
PF03404. Mo-co_dimer. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF00174. Oxidored_molyb. 1 hit.
[Graphical view]
PIRSFiPIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTSiPR00406. CYTB5RDTASE.
PR00363. CYTOCHROMEB5.
PR00407. EUMOPTERIN.
PR00371. FPNCR.
SUPFAMiSSF55856. SSF55856. 1 hit.
SSF56524. SSF56524. 1 hit.
SSF63380. SSF63380. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
PS51384. FAD_FR. 1 hit.
PS00559. MOLYBDOPTERIN_EUK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39870-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAASVDQRPY PGLHNGVVRP LKPGPDIPRP KKLPQAPPPL SDSSSDEEED
60 70 80 90 100
TTLNLKDLIR KGTTEVESSI FDPRDDGTSD HWIQRNSSLV RLTGKHPFNS
110 120 130 140 150
EPPLPRLMHH GFITPVPLHY VRNHGPVPRA RWEDWTVEVT GLVTRPTCFT
160 170 180 190 200
MEQLLHDFPS REFPATLVCA GNRRKEQNMV KQSIGFNWGA AAISTSVWRG
210 220 230 240 250
VPLRTLLKSC GIYTRTKGAL HVCFEGAEDL PGGGGSKYGT SILREVALDP
260 270 280 290 300
SRDIILAYMQ NGEPLSPDHG FPVRMIIPGF IGGRMVKWLK RIIVTTDQSQ
310 320 330 340 350
NYYHYKDNRV LPSHVDAELA NAQAWWYKPD YIINELNINS VITTPCHEEI
360 370 380 390 400
LPINSWTTQM PYFIRGYAYS GGGRKVTRVE VTLDGGETWQ VCTLDCPEKP
410 420 430 440 450
NKYGKYWCWC LWSVEVEVLD LLGAREIAVR AWDEALNTQP EKLIWNVMGM
460 470 480 490 500
MNNCWFRVKT NVCRPHKGEI GIVFEHPTQP GNQSGGWMAK EKHLEKSSES
510 520 530 540 550
NPTLKKSVSS PFMNTTSKTY TMSEVRRHNN ADSAWIIVHG HVYDCTRFLK
560 570 580 590 600
DHPGGTDSIL INAGTDCTEE FEAIHSDKAK QMLEDYRIGE LTTTCYNSDS
610 620 630 640 650
SSSNPSVHGS SDTIPLTPIK EVITPMRSVA LNPREKIPCK LISKTSISHD
660 670 680 690 700
VRLFRFALPS DDLLMGLPVG KHIFLCATVD EKLCMRAYTP TSSVHEVGYF
710 720 730 740 750
DLVVKVYFKG VHPKFPTGGI MSQHLDSLPI GSVLDVKGPL GHIVYTGRGN
760 770 780 790 800
FLVHGKPRFA TRLAMLAGGT GITPIYQVVR AILKDPEDCT EMHVVYANRT
810 820 830 840 850
EDDILLKEEL DEWAKKYDRL KVWYVIQASI REGWEYSVGF ITESILTEHI
860 870 880 890
PNASPDTLAL TCGPPPMIQF AVQPNLEKLG YDTQNNLLVF
Length:890
Mass (Da):99,945
Last modified:February 1, 1995 - v1
Checksum:i3D1B373D9413F78C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U13987 mRNA. Translation: AAA96813.1.
PIRiS66308.
RefSeqiNP_001238150.1. NM_001251221.1.
UniGeneiGma.8416.

Genome annotation databases

GeneIDi732634.
KEGGigmx:732634.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U13987 mRNA. Translation: AAA96813.1.
PIRiS66308.
RefSeqiNP_001238150.1. NM_001251221.1.
UniGeneiGma.8416.

3D structure databases

ProteinModelPortaliP39870.
SMRiP39870. Positions 636-890.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi732634.
KEGGigmx:732634.

Phylogenomic databases

InParanoidiP39870.
KOiK10534.

Gene expression databases

GenevestigatoriP39870.

Family and domain databases

Gene3Di2.60.40.650. 1 hit.
3.10.120.10. 1 hit.
3.90.420.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR014756. Ig_E-set.
IPR005066. MoCF_OxRdtse_dimer.
IPR008335. Mopterin_OxRdtase_euk.
IPR001834. NADH-Cyt_B5_reductase.
IPR012137. Nitr_rd_NADH.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR000572. OxRdtase_Mopterin-bd_dom.
IPR022407. OxRdtase_Mopterin_BS.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
PF00970. FAD_binding_6. 1 hit.
PF03404. Mo-co_dimer. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF00174. Oxidored_molyb. 1 hit.
[Graphical view]
PIRSFiPIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTSiPR00406. CYTB5RDTASE.
PR00363. CYTOCHROMEB5.
PR00407. EUMOPTERIN.
PR00371. FPNCR.
SUPFAMiSSF55856. SSF55856. 1 hit.
SSF56524. SSF56524. 1 hit.
SSF63380. SSF63380. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
PS51384. FAD_FR. 1 hit.
PS00559. MOLYBDOPTERIN_EUK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Identification of cDNA clones corresponding to two inducible nitrate reductase genes in soybean: analysis in wild-type and nr1 mutant."
    Wu S., Lu Q., Kriz A.L., Harper J.E.
    Plant Mol. Biol. 29:491-506(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Williams.

Entry informationi

Entry nameiNIA2_SOYBN
AccessioniPrimary (citable) accession number: P39870
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: January 7, 2015
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.