P39870 (NIA2_SOYBN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inducible nitrate reductase [NADH] 2
Short name=NR
EC=1.7.1.1

Gene names

Name:

INR2

Organism

Glycine max (Soybean) (Glycine hispida) [Reference proteome]

Taxonomic identifier

3847 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Glycine

Protein attributes

Sequence length	890 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.
Catalytic activity	Nitrite + NAD+ + H2O = nitrate + NADH.
Cofactor	Binds 1 FAD. Binds 1 heme group. The heme group is called cytochrome b-557. Binds 1 molybdenum ion.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the nitrate reductase family. Contains 1 cytochrome b5 heme-binding domain. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Biological process	Nitrate assimilation
Ligand	FAD Flavoprotein Heme Iron Metal-binding Molybdenum NAD
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nitrate assimilation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	electron carrier activity Inferred from electronic annotation. Source: InterPro flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro molybdenum ion binding Inferred from electronic annotation. Source: InterPro molybdopterin cofactor binding Inferred from electronic annotation. Source: InterPro nitrate reductase (NADH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 890	890	Inducible nitrate reductase [NADH] 2		PRO_0000166070
Regions
	Domain	517 – 592	76	Cytochrome b5 heme-binding
	Domain	634 – 746	113	FAD-binding FR-type
Sites
	Metal binding	169	1	Molybdenum-pterin Potential
	Metal binding	223	1	Molybdenum-pterin Potential
	Metal binding	552	1	Iron (heme axial ligand) By similarity
	Metal binding	575	1	Iron (heme axial ligand) By similarity
Amino acid modifications
	Disulfide bond	408		Interchain Potential

Sequences

	Sequence		Length	Mass (Da)
	P39870 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 3D1B373D9413F78C	FASTA	890	99,945
	10 20 30 40 50 60 MAASVDQRPY PGLHNGVVRP LKPGPDIPRP KKLPQAPPPL SDSSSDEEED TTLNLKDLIR 70 80 90 100 110 120 KGTTEVESSI FDPRDDGTSD HWIQRNSSLV RLTGKHPFNS EPPLPRLMHH GFITPVPLHY 130 140 150 160 170 180 VRNHGPVPRA RWEDWTVEVT GLVTRPTCFT MEQLLHDFPS REFPATLVCA GNRRKEQNMV 190 200 210 220 230 240 KQSIGFNWGA AAISTSVWRG VPLRTLLKSC GIYTRTKGAL HVCFEGAEDL PGGGGSKYGT 250 260 270 280 290 300 SILREVALDP SRDIILAYMQ NGEPLSPDHG FPVRMIIPGF IGGRMVKWLK RIIVTTDQSQ 310 320 330 340 350 360 NYYHYKDNRV LPSHVDAELA NAQAWWYKPD YIINELNINS VITTPCHEEI LPINSWTTQM 370 380 390 400 410 420 PYFIRGYAYS GGGRKVTRVE VTLDGGETWQ VCTLDCPEKP NKYGKYWCWC LWSVEVEVLD 430 440 450 460 470 480 LLGAREIAVR AWDEALNTQP EKLIWNVMGM MNNCWFRVKT NVCRPHKGEI GIVFEHPTQP 490 500 510 520 530 540 GNQSGGWMAK EKHLEKSSES NPTLKKSVSS PFMNTTSKTY TMSEVRRHNN ADSAWIIVHG 550 560 570 580 590 600 HVYDCTRFLK DHPGGTDSIL INAGTDCTEE FEAIHSDKAK QMLEDYRIGE LTTTCYNSDS 610 620 630 640 650 660 SSSNPSVHGS SDTIPLTPIK EVITPMRSVA LNPREKIPCK LISKTSISHD VRLFRFALPS 670 680 690 700 710 720 DDLLMGLPVG KHIFLCATVD EKLCMRAYTP TSSVHEVGYF DLVVKVYFKG VHPKFPTGGI 730 740 750 760 770 780 MSQHLDSLPI GSVLDVKGPL GHIVYTGRGN FLVHGKPRFA TRLAMLAGGT GITPIYQVVR 790 800 810 820 830 840 AILKDPEDCT EMHVVYANRT EDDILLKEEL DEWAKKYDRL KVWYVIQASI REGWEYSVGF 850 860 870 880 890 ITESILTEHI PNASPDTLAL TCGPPPMIQF AVQPNLEKLG YDTQNNLLVF

References

[1]

"Identification of cDNA clones corresponding to two inducible nitrate reductase genes in soybean: analysis in wild-type and nr1 mutant." Wu S., Lu Q., Kriz A.L., Harper J.E. Plant Mol. Biol. 29:491-506(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Williams.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U13987 mRNA. Translation: AAA96813.1.
PIR	S66308.
RefSeq	NP_001238150.1. NM_001251221.1.
UniGene	Gma.8416.
3D structure databases
ProteinModelPortal	P39870.
SMR	P39870. Positions 636-890.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	732634.
KEGG	gmx:732634.
Gene expression databases
Genevestigator	P39870.
Family and domain databases
Gene3D	2.60.40.650. 1 hit. 3.10.120.10. 1 hit. 3.90.420.10. 1 hit.
InterPro	IPR001199. Cyt_B5-like_heme/steroid-bd. IPR018506. Cyt_B5_heme-BS. IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR014756. Ig_E-set. IPR005066. MoCF_OxRdtse_dimer. IPR008335. Mopterin_OxRdtase_euk. IPR001834. NADH-Cyt_B5_reductase. IPR012137. Nitr_rd_NADH. IPR008333. OxRdtase_FAD-bd_dom. IPR001433. OxRdtase_FAD/NAD-bd. IPR000572. OxRdtase_Mopterin-bd_dom. IPR022407. OxRdtase_Mopterin_BS. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
Pfam	PF00173. Cyt-b5. 1 hit. PF00970. FAD_binding_6. 1 hit. PF03404. Mo-co_dimer. 1 hit. PF00175. NAD_binding_1. 1 hit. PF00174. Oxidored_molyb. 1 hit. [Graphical view]
PIRSF	PIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTS	PR00406. CYTB5RDTASE. PR00363. CYTOCHROMEB5. PR00407. EUMOPTERIN. PR00371. FPNCR.
SUPFAM	SSF55856. Cyt_B5. 1 hit. SSF81296. Ig_E-set. 1 hit. SSF56524. Oxidored_molyb. 1 hit. SSF63380. Riboflavin_synthase_like_b-brl. 1 hit.
PROSITE	PS00191. CYTOCHROME_B5_1. 1 hit. PS50255. CYTOCHROME_B5_2. 1 hit. PS51384. FAD_FR. 1 hit. PS00559. MOLYBDOPTERIN_EUK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NIA2_SOYBN

Accession

Primary (citable) accession number: P39870

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	April 3, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program