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P39870 (NIA2_SOYBN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Inducible nitrate reductase [NADH] 2

Short name=NR
EC=1.7.1.1
Gene names
Name:INR2
OrganismGlycine max (Soybean) (Glycine hispida) [Reference proteome]
Taxonomic identifier3847 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja

Protein attributes

Sequence length890 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.

Catalytic activity

Nitrite + NAD+ + H2O = nitrate + NADH.

Cofactor

Binds 1 FAD By similarity.

Binds 1 heme group. The heme group is called cytochrome b-557 By similarity.

Binds 1 molybdenum-molybdopterin (Mo-MPT) cofactor per subunit By similarity.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the nitrate reductase family.

Contains 1 cytochrome b5 heme-binding domain.

Contains 1 FAD-binding FR-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 890890Inducible nitrate reductase [NADH] 2
PRO_0000166070

Regions

Domain517 – 59276Cytochrome b5 heme-binding
Domain634 – 746113FAD-binding FR-type

Sites

Metal binding1691Molybdenum By similarity
Metal binding5521Iron (heme axial ligand) By similarity
Metal binding5751Iron (heme axial ligand) By similarity

Amino acid modifications

Disulfide bond408Interchain Potential

Sequences

Sequence LengthMass (Da)Tools
P39870 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 3D1B373D9413F78C

FASTA89099,945
        10         20         30         40         50         60 
MAASVDQRPY PGLHNGVVRP LKPGPDIPRP KKLPQAPPPL SDSSSDEEED TTLNLKDLIR 

        70         80         90        100        110        120 
KGTTEVESSI FDPRDDGTSD HWIQRNSSLV RLTGKHPFNS EPPLPRLMHH GFITPVPLHY 

       130        140        150        160        170        180 
VRNHGPVPRA RWEDWTVEVT GLVTRPTCFT MEQLLHDFPS REFPATLVCA GNRRKEQNMV 

       190        200        210        220        230        240 
KQSIGFNWGA AAISTSVWRG VPLRTLLKSC GIYTRTKGAL HVCFEGAEDL PGGGGSKYGT 

       250        260        270        280        290        300 
SILREVALDP SRDIILAYMQ NGEPLSPDHG FPVRMIIPGF IGGRMVKWLK RIIVTTDQSQ 

       310        320        330        340        350        360 
NYYHYKDNRV LPSHVDAELA NAQAWWYKPD YIINELNINS VITTPCHEEI LPINSWTTQM 

       370        380        390        400        410        420 
PYFIRGYAYS GGGRKVTRVE VTLDGGETWQ VCTLDCPEKP NKYGKYWCWC LWSVEVEVLD 

       430        440        450        460        470        480 
LLGAREIAVR AWDEALNTQP EKLIWNVMGM MNNCWFRVKT NVCRPHKGEI GIVFEHPTQP 

       490        500        510        520        530        540 
GNQSGGWMAK EKHLEKSSES NPTLKKSVSS PFMNTTSKTY TMSEVRRHNN ADSAWIIVHG 

       550        560        570        580        590        600 
HVYDCTRFLK DHPGGTDSIL INAGTDCTEE FEAIHSDKAK QMLEDYRIGE LTTTCYNSDS 

       610        620        630        640        650        660 
SSSNPSVHGS SDTIPLTPIK EVITPMRSVA LNPREKIPCK LISKTSISHD VRLFRFALPS 

       670        680        690        700        710        720 
DDLLMGLPVG KHIFLCATVD EKLCMRAYTP TSSVHEVGYF DLVVKVYFKG VHPKFPTGGI 

       730        740        750        760        770        780 
MSQHLDSLPI GSVLDVKGPL GHIVYTGRGN FLVHGKPRFA TRLAMLAGGT GITPIYQVVR 

       790        800        810        820        830        840 
AILKDPEDCT EMHVVYANRT EDDILLKEEL DEWAKKYDRL KVWYVIQASI REGWEYSVGF 

       850        860        870        880        890 
ITESILTEHI PNASPDTLAL TCGPPPMIQF AVQPNLEKLG YDTQNNLLVF 

« Hide

References

[1]"Identification of cDNA clones corresponding to two inducible nitrate reductase genes in soybean: analysis in wild-type and nr1 mutant."
Wu S., Lu Q., Kriz A.L., Harper J.E.
Plant Mol. Biol. 29:491-506(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Williams.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U13987 mRNA. Translation: AAA96813.1.
PIRS66308.
RefSeqNP_001238150.1. NM_001251221.1.
UniGeneGma.8416.

3D structure databases

ProteinModelPortalP39870.
SMRP39870. Positions 636-890.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID732634.
KEGGgmx:732634.

Phylogenomic databases

KOK10534.

Gene expression databases

GenevestigatorP39870.

Family and domain databases

Gene3D2.60.40.650. 1 hit.
3.10.120.10. 1 hit.
3.90.420.10. 1 hit.
InterProIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR014756. Ig_E-set.
IPR005066. MoCF_OxRdtse_dimer.
IPR008335. Mopterin_OxRdtase_euk.
IPR001834. NADH-Cyt_B5_reductase.
IPR012137. Nitr_rd_NADH.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR000572. OxRdtase_Mopterin-bd_dom.
IPR022407. OxRdtase_Mopterin_BS.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamPF00173. Cyt-b5. 1 hit.
PF00970. FAD_binding_6. 1 hit.
PF03404. Mo-co_dimer. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF00174. Oxidored_molyb. 1 hit.
[Graphical view]
PIRSFPIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTSPR00406. CYTB5RDTASE.
PR00363. CYTOCHROMEB5.
PR00407. EUMOPTERIN.
PR00371. FPNCR.
SUPFAMSSF55856. SSF55856. 1 hit.
SSF56524. SSF56524. 1 hit.
SSF63380. SSF63380. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
PS51384. FAD_FR. 1 hit.
PS00559. MOLYBDOPTERIN_EUK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNIA2_SOYBN
AccessionPrimary (citable) accession number: P39870
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families