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P39870

- NIA2_SOYBN

UniProt

P39870 - NIA2_SOYBN

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Protein
Inducible nitrate reductase [NADH] 2
Gene
INR2
Organism
Glycine max (Soybean) (Glycine hispida)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.

Catalytic activityi

Nitrite + NAD+ + H2O = nitrate + NADH.

Cofactori

Binds 1 FAD By similarity.
Binds 1 heme group. The heme group is called cytochrome b-557 By similarity.
Binds 1 molybdenum-molybdopterin (Mo-MPT) cofactor per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi169 – 1691Molybdenum By similarity
Metal bindingi552 – 5521Iron (heme axial ligand) By similarity
Metal bindingi575 – 5751Iron (heme axial ligand) By similarity

GO - Molecular functioni

  1. electron carrier activity Source: InterPro
  2. flavin adenine dinucleotide binding Source: InterPro
  3. heme binding Source: InterPro
  4. iron ion binding Source: InterPro
  5. molybdenum ion binding Source: InterPro
  6. molybdopterin cofactor binding Source: InterPro
  7. nitrate reductase (NADH) activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. nitrate assimilation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Nitrate assimilation

Keywords - Ligandi

FAD, Flavoprotein, Heme, Iron, Metal-binding, Molybdenum, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Inducible nitrate reductase [NADH] 2 (EC:1.7.1.1)
Short name:
NR
Gene namesi
Name:INR2
OrganismiGlycine max (Soybean) (Glycine hispida)
Taxonomic identifieri3847 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja
ProteomesiUP000008827: Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 890890Inducible nitrate reductase [NADH] 2
PRO_0000166070Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi408 – 408Interchain Reviewed prediction

Keywords - PTMi

Disulfide bond

Expressioni

Gene expression databases

GenevestigatoriP39870.

Interactioni

Subunit structurei

Homodimer By similarity.

Structurei

3D structure databases

ProteinModelPortaliP39870.
SMRiP39870. Positions 636-890.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini517 – 59276Cytochrome b5 heme-binding
Add
BLAST
Domaini634 – 746113FAD-binding FR-type
Add
BLAST

Sequence similaritiesi

Belongs to the nitrate reductase family.

Phylogenomic databases

KOiK10534.

Family and domain databases

Gene3Di2.60.40.650. 1 hit.
3.10.120.10. 1 hit.
3.90.420.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR014756. Ig_E-set.
IPR005066. MoCF_OxRdtse_dimer.
IPR008335. Mopterin_OxRdtase_euk.
IPR001834. NADH-Cyt_B5_reductase.
IPR012137. Nitr_rd_NADH.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR000572. OxRdtase_Mopterin-bd_dom.
IPR022407. OxRdtase_Mopterin_BS.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
PF00970. FAD_binding_6. 1 hit.
PF03404. Mo-co_dimer. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF00174. Oxidored_molyb. 1 hit.
[Graphical view]
PIRSFiPIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTSiPR00406. CYTB5RDTASE.
PR00363. CYTOCHROMEB5.
PR00407. EUMOPTERIN.
PR00371. FPNCR.
SUPFAMiSSF55856. SSF55856. 1 hit.
SSF56524. SSF56524. 1 hit.
SSF63380. SSF63380. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
PS51384. FAD_FR. 1 hit.
PS00559. MOLYBDOPTERIN_EUK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39870-1 [UniParc]FASTAAdd to Basket

« Hide

MAASVDQRPY PGLHNGVVRP LKPGPDIPRP KKLPQAPPPL SDSSSDEEED    50
TTLNLKDLIR KGTTEVESSI FDPRDDGTSD HWIQRNSSLV RLTGKHPFNS 100
EPPLPRLMHH GFITPVPLHY VRNHGPVPRA RWEDWTVEVT GLVTRPTCFT 150
MEQLLHDFPS REFPATLVCA GNRRKEQNMV KQSIGFNWGA AAISTSVWRG 200
VPLRTLLKSC GIYTRTKGAL HVCFEGAEDL PGGGGSKYGT SILREVALDP 250
SRDIILAYMQ NGEPLSPDHG FPVRMIIPGF IGGRMVKWLK RIIVTTDQSQ 300
NYYHYKDNRV LPSHVDAELA NAQAWWYKPD YIINELNINS VITTPCHEEI 350
LPINSWTTQM PYFIRGYAYS GGGRKVTRVE VTLDGGETWQ VCTLDCPEKP 400
NKYGKYWCWC LWSVEVEVLD LLGAREIAVR AWDEALNTQP EKLIWNVMGM 450
MNNCWFRVKT NVCRPHKGEI GIVFEHPTQP GNQSGGWMAK EKHLEKSSES 500
NPTLKKSVSS PFMNTTSKTY TMSEVRRHNN ADSAWIIVHG HVYDCTRFLK 550
DHPGGTDSIL INAGTDCTEE FEAIHSDKAK QMLEDYRIGE LTTTCYNSDS 600
SSSNPSVHGS SDTIPLTPIK EVITPMRSVA LNPREKIPCK LISKTSISHD 650
VRLFRFALPS DDLLMGLPVG KHIFLCATVD EKLCMRAYTP TSSVHEVGYF 700
DLVVKVYFKG VHPKFPTGGI MSQHLDSLPI GSVLDVKGPL GHIVYTGRGN 750
FLVHGKPRFA TRLAMLAGGT GITPIYQVVR AILKDPEDCT EMHVVYANRT 800
EDDILLKEEL DEWAKKYDRL KVWYVIQASI REGWEYSVGF ITESILTEHI 850
PNASPDTLAL TCGPPPMIQF AVQPNLEKLG YDTQNNLLVF 890
Length:890
Mass (Da):99,945
Last modified:February 1, 1995 - v1
Checksum:i3D1B373D9413F78C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U13987 mRNA. Translation: AAA96813.1.
PIRiS66308.
RefSeqiNP_001238150.1. NM_001251221.1.
UniGeneiGma.8416.

Genome annotation databases

GeneIDi732634.
KEGGigmx:732634.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U13987 mRNA. Translation: AAA96813.1 .
PIRi S66308.
RefSeqi NP_001238150.1. NM_001251221.1.
UniGenei Gma.8416.

3D structure databases

ProteinModelPortali P39870.
SMRi P39870. Positions 636-890.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 732634.
KEGGi gmx:732634.

Phylogenomic databases

KOi K10534.

Gene expression databases

Genevestigatori P39870.

Family and domain databases

Gene3Di 2.60.40.650. 1 hit.
3.10.120.10. 1 hit.
3.90.420.10. 1 hit.
InterProi IPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR014756. Ig_E-set.
IPR005066. MoCF_OxRdtse_dimer.
IPR008335. Mopterin_OxRdtase_euk.
IPR001834. NADH-Cyt_B5_reductase.
IPR012137. Nitr_rd_NADH.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR000572. OxRdtase_Mopterin-bd_dom.
IPR022407. OxRdtase_Mopterin_BS.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view ]
Pfami PF00173. Cyt-b5. 1 hit.
PF00970. FAD_binding_6. 1 hit.
PF03404. Mo-co_dimer. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF00174. Oxidored_molyb. 1 hit.
[Graphical view ]
PIRSFi PIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTSi PR00406. CYTB5RDTASE.
PR00363. CYTOCHROMEB5.
PR00407. EUMOPTERIN.
PR00371. FPNCR.
SUPFAMi SSF55856. SSF55856. 1 hit.
SSF56524. SSF56524. 1 hit.
SSF63380. SSF63380. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEi PS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
PS51384. FAD_FR. 1 hit.
PS00559. MOLYBDOPTERIN_EUK. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Identification of cDNA clones corresponding to two inducible nitrate reductase genes in soybean: analysis in wild-type and nr1 mutant."
    Wu S., Lu Q., Kriz A.L., Harper J.E.
    Plant Mol. Biol. 29:491-506(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Williams.

Entry informationi

Entry nameiNIA2_SOYBN
AccessioniPrimary (citable) accession number: P39870
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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