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P39870

- NIA2_SOYBN

UniProt

P39870 - NIA2_SOYBN

Protein

Inducible nitrate reductase [NADH] 2

Gene

INR2

Organism
Glycine max (Soybean) (Glycine hispida)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.

    Catalytic activityi

    Nitrite + NAD+ + H2O = nitrate + NADH.

    Cofactori

    Binds 1 FAD.By similarity
    Binds 1 heme group. The heme group is called cytochrome b-557 By similarity.By similarity
    Binds 1 molybdenum-molybdopterin (Mo-MPT) cofactor per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi169 – 1691MolybdenumBy similarity
    Metal bindingi552 – 5521Iron (heme axial ligand)PROSITE-ProRule annotation
    Metal bindingi575 – 5751Iron (heme axial ligand)PROSITE-ProRule annotation

    GO - Molecular functioni

    1. electron carrier activity Source: InterPro
    2. flavin adenine dinucleotide binding Source: InterPro
    3. heme binding Source: InterPro
    4. iron ion binding Source: InterPro
    5. molybdenum ion binding Source: InterPro
    6. molybdopterin cofactor binding Source: InterPro
    7. nitrate reductase (NADH) activity Source: UniProtKB-EC

    GO - Biological processi

    1. nitrate assimilation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Nitrate assimilation

    Keywords - Ligandi

    FAD, Flavoprotein, Heme, Iron, Metal-binding, Molybdenum, NAD

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inducible nitrate reductase [NADH] 2 (EC:1.7.1.1)
    Short name:
    NR
    Gene namesi
    Name:INR2
    OrganismiGlycine max (Soybean) (Glycine hispida)
    Taxonomic identifieri3847 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja
    ProteomesiUP000008827: Unplaced

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 890890Inducible nitrate reductase [NADH] 2PRO_0000166070Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi408 – 408InterchainSequence Analysis

    Keywords - PTMi

    Disulfide bond

    Expressioni

    Gene expression databases

    GenevestigatoriP39870.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP39870.
    SMRiP39870. Positions 636-890.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini517 – 59276Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini634 – 746113FAD-binding FR-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the nitrate reductase family.Curated
    Contains 1 cytochrome b5 heme-binding domain.PROSITE-ProRule annotation
    Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation

    Phylogenomic databases

    KOiK10534.

    Family and domain databases

    Gene3Di2.60.40.650. 1 hit.
    3.10.120.10. 1 hit.
    3.90.420.10. 1 hit.
    InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
    IPR018506. Cyt_B5_heme-BS.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR014756. Ig_E-set.
    IPR005066. MoCF_OxRdtse_dimer.
    IPR008335. Mopterin_OxRdtase_euk.
    IPR001834. NADH-Cyt_B5_reductase.
    IPR012137. Nitr_rd_NADH.
    IPR008333. OxRdtase_FAD-bd_dom.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR000572. OxRdtase_Mopterin-bd_dom.
    IPR022407. OxRdtase_Mopterin_BS.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view]
    PfamiPF00173. Cyt-b5. 1 hit.
    PF00970. FAD_binding_6. 1 hit.
    PF03404. Mo-co_dimer. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    PF00174. Oxidored_molyb. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000233. Nitr_rd_NADH. 1 hit.
    PRINTSiPR00406. CYTB5RDTASE.
    PR00363. CYTOCHROMEB5.
    PR00407. EUMOPTERIN.
    PR00371. FPNCR.
    SUPFAMiSSF55856. SSF55856. 1 hit.
    SSF56524. SSF56524. 1 hit.
    SSF63380. SSF63380. 1 hit.
    SSF81296. SSF81296. 1 hit.
    PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
    PS50255. CYTOCHROME_B5_2. 1 hit.
    PS51384. FAD_FR. 1 hit.
    PS00559. MOLYBDOPTERIN_EUK. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P39870-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAASVDQRPY PGLHNGVVRP LKPGPDIPRP KKLPQAPPPL SDSSSDEEED    50
    TTLNLKDLIR KGTTEVESSI FDPRDDGTSD HWIQRNSSLV RLTGKHPFNS 100
    EPPLPRLMHH GFITPVPLHY VRNHGPVPRA RWEDWTVEVT GLVTRPTCFT 150
    MEQLLHDFPS REFPATLVCA GNRRKEQNMV KQSIGFNWGA AAISTSVWRG 200
    VPLRTLLKSC GIYTRTKGAL HVCFEGAEDL PGGGGSKYGT SILREVALDP 250
    SRDIILAYMQ NGEPLSPDHG FPVRMIIPGF IGGRMVKWLK RIIVTTDQSQ 300
    NYYHYKDNRV LPSHVDAELA NAQAWWYKPD YIINELNINS VITTPCHEEI 350
    LPINSWTTQM PYFIRGYAYS GGGRKVTRVE VTLDGGETWQ VCTLDCPEKP 400
    NKYGKYWCWC LWSVEVEVLD LLGAREIAVR AWDEALNTQP EKLIWNVMGM 450
    MNNCWFRVKT NVCRPHKGEI GIVFEHPTQP GNQSGGWMAK EKHLEKSSES 500
    NPTLKKSVSS PFMNTTSKTY TMSEVRRHNN ADSAWIIVHG HVYDCTRFLK 550
    DHPGGTDSIL INAGTDCTEE FEAIHSDKAK QMLEDYRIGE LTTTCYNSDS 600
    SSSNPSVHGS SDTIPLTPIK EVITPMRSVA LNPREKIPCK LISKTSISHD 650
    VRLFRFALPS DDLLMGLPVG KHIFLCATVD EKLCMRAYTP TSSVHEVGYF 700
    DLVVKVYFKG VHPKFPTGGI MSQHLDSLPI GSVLDVKGPL GHIVYTGRGN 750
    FLVHGKPRFA TRLAMLAGGT GITPIYQVVR AILKDPEDCT EMHVVYANRT 800
    EDDILLKEEL DEWAKKYDRL KVWYVIQASI REGWEYSVGF ITESILTEHI 850
    PNASPDTLAL TCGPPPMIQF AVQPNLEKLG YDTQNNLLVF 890
    Length:890
    Mass (Da):99,945
    Last modified:February 1, 1995 - v1
    Checksum:i3D1B373D9413F78C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U13987 mRNA. Translation: AAA96813.1.
    PIRiS66308.
    RefSeqiNP_001238150.1. NM_001251221.1.
    UniGeneiGma.8416.

    Genome annotation databases

    GeneIDi732634.
    KEGGigmx:732634.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U13987 mRNA. Translation: AAA96813.1 .
    PIRi S66308.
    RefSeqi NP_001238150.1. NM_001251221.1.
    UniGenei Gma.8416.

    3D structure databases

    ProteinModelPortali P39870.
    SMRi P39870. Positions 636-890.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 732634.
    KEGGi gmx:732634.

    Phylogenomic databases

    KOi K10534.

    Gene expression databases

    Genevestigatori P39870.

    Family and domain databases

    Gene3Di 2.60.40.650. 1 hit.
    3.10.120.10. 1 hit.
    3.90.420.10. 1 hit.
    InterProi IPR001199. Cyt_B5-like_heme/steroid-bd.
    IPR018506. Cyt_B5_heme-BS.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR014756. Ig_E-set.
    IPR005066. MoCF_OxRdtse_dimer.
    IPR008335. Mopterin_OxRdtase_euk.
    IPR001834. NADH-Cyt_B5_reductase.
    IPR012137. Nitr_rd_NADH.
    IPR008333. OxRdtase_FAD-bd_dom.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR000572. OxRdtase_Mopterin-bd_dom.
    IPR022407. OxRdtase_Mopterin_BS.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view ]
    Pfami PF00173. Cyt-b5. 1 hit.
    PF00970. FAD_binding_6. 1 hit.
    PF03404. Mo-co_dimer. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    PF00174. Oxidored_molyb. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000233. Nitr_rd_NADH. 1 hit.
    PRINTSi PR00406. CYTB5RDTASE.
    PR00363. CYTOCHROMEB5.
    PR00407. EUMOPTERIN.
    PR00371. FPNCR.
    SUPFAMi SSF55856. SSF55856. 1 hit.
    SSF56524. SSF56524. 1 hit.
    SSF63380. SSF63380. 1 hit.
    SSF81296. SSF81296. 1 hit.
    PROSITEi PS00191. CYTOCHROME_B5_1. 1 hit.
    PS50255. CYTOCHROME_B5_2. 1 hit.
    PS51384. FAD_FR. 1 hit.
    PS00559. MOLYBDOPTERIN_EUK. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of cDNA clones corresponding to two inducible nitrate reductase genes in soybean: analysis in wild-type and nr1 mutant."
      Wu S., Lu Q., Kriz A.L., Harper J.E.
      Plant Mol. Biol. 29:491-506(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Williams.

    Entry informationi

    Entry nameiNIA2_SOYBN
    AccessioniPrimary (citable) accession number: P39870
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 98 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3