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Protein

Nitrate reductase [NADH]

Gene

NIA

Organism
Lotus japonicus (Lotus corniculatus var. japonicus)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.

Catalytic activityi

Nitrite + NAD+ + H2O = nitrate + NADH.

Cofactori

Protein has several cofactor binding sites:
  • FADBy similarityNote: Binds 1 FAD.By similarity
  • hemeBy similarityNote: Binds 1 heme group. The heme group is called cytochrome b-557.By similarity
  • Mo-molybdopterinBy similarityNote: Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi172MolybdenumBy similarity1
Metal bindingi556Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi579Iron (heme axial ligand)PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processNitrate assimilation
LigandFAD, Flavoprotein, Heme, Iron, Metal-binding, Molybdenum, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate reductase [NADH] (EC:1.7.1.1)
Short name:
NR
Gene namesi
Name:NIA
OrganismiLotus japonicus (Lotus corniculatus var. japonicus)
Taxonomic identifieri34305 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeLoteaeLotus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001660591 – 900Nitrate reductase [NADH]Add BLAST900

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi411InterchainSequence analysis

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP39869

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP39869
SMRiP39869
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini521 – 596Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd BLAST76
Domaini644 – 756FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST113

Sequence similaritiesi

Belongs to the nitrate reductase family.Curated

Family and domain databases

Gene3Di3.10.120.10, 1 hit
3.90.420.10, 1 hit
InterProiView protein in InterPro
IPR001199 Cyt_B5-like_heme/steroid-bd
IPR036400 Cyt_B5-like_heme/steroid_sf
IPR018506 Cyt_B5_heme-BS
IPR017927 Fd_Rdtase_FAD-bd
IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR014756 Ig_E-set
IPR005066 MoCF_OxRdtse_dimer
IPR008335 Mopterin_OxRdtase_euk
IPR001834 NADH-Cyt_B5_reductase
IPR012137 Nitr_rd_NADH
IPR008333 OxRdtase_FAD-bd_dom
IPR001433 OxRdtase_FAD/NAD-bd
IPR000572 OxRdtase_Mopterin-bd_dom
IPR036374 OxRdtase_Mopterin-bd_sf
IPR022407 OxRdtase_Mopterin_BS
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00173 Cyt-b5, 1 hit
PF00970 FAD_binding_6, 1 hit
PF03404 Mo-co_dimer, 1 hit
PF00175 NAD_binding_1, 1 hit
PF00174 Oxidored_molyb, 1 hit
PIRSFiPIRSF000233 Nitr_rd_NADH, 1 hit
PRINTSiPR00406 CYTB5RDTASE
PR00363 CYTOCHROMEB5
PR00407 EUMOPTERIN
PR00371 FPNCR
SMARTiView protein in SMART
SM01117 Cyt-b5, 1 hit
SUPFAMiSSF55856 SSF55856, 1 hit
SSF56524 SSF56524, 1 hit
SSF63380 SSF63380, 1 hit
SSF81296 SSF81296, 1 hit
PROSITEiView protein in PROSITE
PS00191 CYTOCHROME_B5_1, 1 hit
PS50255 CYTOCHROME_B5_2, 1 hit
PS51384 FAD_FR, 1 hit
PS00559 MOLYBDOPTERIN_EUK, 1 hit

Sequencei

Sequence statusi: Complete.

P39869-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASVDNRQY STLNGVVRSF TPNNTYLHDP KPSFPAVNLD LDASSSDDDD
60 70 80 90 100
EKDDASILKD LIRKGNAEIE SSVLDPRDQG TADNWISRNS SMVRLTGKHP
110 120 130 140 150
FNSEPPLPRL MHHGFITPVP LHYVRNHGPV PKARWDDWTV EVTGLVKTPT
160 170 180 190 200
RFSMDRLVRD FPSRELPVTL VCAGNRRKEQ NMVRQSIGFN WGSAGVSTSV
210 220 230 240 250
WRGVSLRHIL RRCRIQTRSR GALHVCFEGD EDLPGGGGSK YSTSIRREVA
260 270 280 290 300
MDPSRDVILA YMQNGEVLAP DHGFPVRVII PGFIGGRMVK WLKRIVVTEE
310 320 330 340 350
ECDGHYHYKD NRVLPSHVDA ELANEEGWWY KPEYIINELN INSVITTPCH
360 370 380 390 400
DEILPINAWT TQRPYTLRGY SYSGGGRKVT RVEVTLDGGE TWFVCALDQQ
410 420 430 440 450
EKPNKYGKYW CWCFWSLEVE VLDLLGTKEI AVRAWDEALN TQPENLIWNV
460 470 480 490 500
MGMMNNCWFR VKTNVCKPHK GEIGIVFEHP TQPGNQPGGW MAKEKHLEIS
510 520 530 540 550
QQDSRPILKK SVSSPFMNTF TKMYSLSEVK KHNSPDSAWI IVHGHVYDCT
560 570 580 590 600
RFLKDHPGGA DSILINAGTD CTEEFEAIHS DKAKKMLEDY RVGELITTGY
610 620 630 640 650
TSDSSSPNNS LHGNSEFKHL APIKEITTMS LPPLPRRKVA LIPREKIPCK
660 670 680 690 700
LISRTSISHD VRVFRFALPS EDQQLGLPVG KHIFLCATVD GKLCMRAYTP
710 720 730 740 750
TSGVDEVGYF ELVVKVYFKG VHPKFPNGGA MSQHLDSLPI GSDLDVKGPL
760 770 780 790 800
GHIEYTGRGN FLVHGKHRFA KKLAMLAGGT GITPIYQVAQ AILKDPEDHT
810 820 830 840 850
KMYVVYANRT EDDILLREEL DTWAKKYEDR FKVWYVVETA KEGWGYSVGF
860 870 880 890 900
VTEGVMREHL PEAGDDALAL ACGPPPMIQF AVNPNLEKMG YDVKNDLLVF
Length:900
Mass (Da):101,420
Last modified:February 1, 1995 - v1
Checksum:i547C25388DD13535
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80670 Genomic DNA Translation: CAA56696.1
PIRiS47029

Similar proteinsi

Entry informationi

Entry nameiNIA_LOTJA
AccessioniPrimary (citable) accession number: P39869
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

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