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Protein

Nitrate reductase [NADH], clone PBNBR1412

Gene

NIA2

Organism
Brassica napus (Rape)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.

Catalytic activityi

Nitrite + NAD+ + H2O = nitrate + NADH.

Cofactori

Protein has several cofactor binding sites:
  • FADBy similarityNote: Binds 1 FAD.By similarity
  • hemeBy similarityNote: Binds 1 heme group. The heme group is called cytochrome b-557.By similarity
  • Mo-molybdopterinBy similarityNote: Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi191MolybdenumBy similarity1
Metal bindingi574Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi597Iron (heme axial ligand)PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processNitrate assimilation
LigandFAD, Flavoprotein, Heme, Iron, Metal-binding, Molybdenum, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate reductase [NADH], clone PBNBR1412 (EC:1.7.1.1)
Short name:
NR
Gene namesi
Name:NIA2
OrganismiBrassica napus (Rape)
Taxonomic identifieri3708 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001660521 – 911Nitrate reductase [NADH], clone PBNBR1412Add BLAST911

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi430InterchainSequence analysis

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP39868

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP39868
SMRiP39868
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini539 – 614Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd BLAST76
Domaini654 – 766FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST113

Sequence similaritiesi

Belongs to the nitrate reductase family.Curated

Phylogenomic databases

KOiK10534

Family and domain databases

Gene3Di3.10.120.10, 1 hit
3.90.420.10, 1 hit
InterProiView protein in InterPro
IPR001199 Cyt_B5-like_heme/steroid-bd
IPR036400 Cyt_B5-like_heme/steroid_sf
IPR018506 Cyt_B5_heme-BS
IPR017927 Fd_Rdtase_FAD-bd
IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR014756 Ig_E-set
IPR005066 MoCF_OxRdtse_dimer
IPR008335 Mopterin_OxRdtase_euk
IPR001834 NADH-Cyt_B5_reductase
IPR012137 Nitr_rd_NADH
IPR008333 OxRdtase_FAD-bd_dom
IPR001433 OxRdtase_FAD/NAD-bd
IPR000572 OxRdtase_Mopterin-bd_dom
IPR036374 OxRdtase_Mopterin-bd_sf
IPR022407 OxRdtase_Mopterin_BS
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00173 Cyt-b5, 1 hit
PF00970 FAD_binding_6, 1 hit
PF03404 Mo-co_dimer, 1 hit
PF00175 NAD_binding_1, 1 hit
PF00174 Oxidored_molyb, 1 hit
PIRSFiPIRSF000233 Nitr_rd_NADH, 1 hit
PRINTSiPR00406 CYTB5RDTASE
PR00363 CYTOCHROMEB5
PR00407 EUMOPTERIN
PR00371 FPNCR
SMARTiView protein in SMART
SM01117 Cyt-b5, 1 hit
SUPFAMiSSF55856 SSF55856, 1 hit
SSF56524 SSF56524, 1 hit
SSF63380 SSF63380, 1 hit
SSF81296 SSF81296, 1 hit
PROSITEiView protein in PROSITE
PS00191 CYTOCHROME_B5_1, 1 hit
PS50255 CYTOCHROME_B5_2, 1 hit
PS51384 FAD_FR, 1 hit
PS00559 MOLYBDOPTERIN_EUK, 1 hit

Sequencei

Sequence statusi: Complete.

P39868-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATSVDNRHY PRLNPAINGV VRSFKPPPIP SPRHQNKTVS FLTEKVIVKE
60 70 80 90 100
TKNDAVDDSY DSSDDEDESH NRNVSYYKEM IRKSHSDVEP SILDSRDEST
110 120 130 140 150
ADNWIHRNSS MVRLTGKHPF NAEPPLPRLM HHGFITPVPL HYVRNHGTVP
160 170 180 190 200
KADWSEWTVE ITGLVKRPAK FTMEELISEF PSREFPVTLV CAGNRRKEQN
210 220 230 240 250
MVKQTIGFNW GSAGVSTSLW KGVPLSEILG RCGIYSRRGG GLNVCFEGAE
260 270 280 290 300
DLPGGGGSKY GTSIKKEMAM DPARDIILAY MQNGELLTPD HGFPVRIIVP
310 320 330 340 350
GFIGGRMVKW LKRIIVTPQE SDSYYHYKDN RVLPSLVDAE LANAEAWWYK
360 370 380 390 400
PEYIINELNI NSVITTPGHQ EILPINAFTT QKPYTLKGYA YSGGGKKVTR
410 420 430 440 450
VEVTLDGGDT WSVCDLDHQE KPNKYGKFWC WCFWSLDVEV LDLLSAKDVA
460 470 480 490 500
VRAWDESFNT QPDKLIWNLM GMMNNCWFRI KTNVCKPHKG EIGIVFEHPT
510 520 530 540 550
RPGNQSGGWM AKERQLEISS ESNPILKKSV SSPFMNTSAK MYSMSEVRKH
560 570 580 590 600
NSVESAWIIV HGHIYDCTRF LKDHPGGSDS ILINAGTDCT EEFEAIHSDK
610 620 630 640 650
AKKLLEDYRI GELITTGYDS SPNVSVHGGS TAVSLLAPIK ELAPSKNIAL
660 670 680 690 700
VNPREKVPVT LIEKTSISHD VRRFRFALPS EDQQLGLPVG KHIFLCANIN
710 720 730 740 750
DKLCLRAYTP TSTVDAVGHI DLVVKVYFKD VHPRFPNGGL MSQHLDSLPI
760 770 780 790 800
GSVLNIKGPL GHIEYLGKGN FMVTGKPKFA KKLAMLAGGP GITPIYQVIQ
810 820 830 840 850
SILSDPEDET EMFVVYANRT EDDILVREEL EGWANKHKDR LKVWYVVEIA
860 870 880 890 900
KEGWNYSTGF ITEPVLREHV PEGLEGESLA LACGPPPMIQ FALQPNLEKM
910
GYNVKEDLLI F
Length:911
Mass (Da):102,201
Last modified:February 1, 1995 - v1
Checksum:iBA06FEC14114DC21
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38220 mRNA Translation: BAA07395.1
PIRiT08108
RefSeqiNP_001303048.1, NM_001316119.1
UniGeneiBna.1439

Genome annotation databases

GeneIDi106368948
KEGGibna:106368948

Similar proteinsi

Entry informationi

Entry nameiNIA2_BRANA
AccessioniPrimary (citable) accession number: P39868
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

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