Reviewed, UniProtKB/Swiss-Prot P39868 (NIA2_BRANA)

Names and origin

Protein names

Recommended name:
    Nitrate reductase [NADH], clone PBNBR1412
      Short name=NR
    EC=1.7.1.1

Gene names

Name:

NIA2

Organism

Brassica napus (Rape)

Taxonomic identifier

3708 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Brassica

Protein attributes

Sequence length	911 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.
Catalytic activity	Nitrite + NAD(+) + H(2)O = nitrate + NADH.
Cofactor	Binds 1 FAD. Binds 1 heme group. The heme group is called cytochrome b-557. Binds 1 molybdenum ion.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the nitrate reductase family. Contains 1 cytochrome b5 heme-binding domain. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Biological process	Nitrate assimilation
Ligand	FAD Flavoprotein Heme Iron Metal-binding Molybdenum NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	nitrate assimilation Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro molybdenum ion binding Inferred from electronic annotation. Source: InterPro nitrate reductase (NADH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 911	911	Nitrate reductase [NADH], clone PBNBR1412		PRO_0000166052
Regions
	Domain	539 – 614	76	Cytochrome b5 heme-binding
	Domain	654 – 766	113	FAD-binding FR-type
Sites
	Metal binding	191	1	Molybdenum-pterin Potential
	Metal binding	245	1	Molybdenum-pterin Potential
	Metal binding	574	1	Iron (heme axial ligand) By similarity
	Metal binding	597	1	Iron (heme axial ligand) By similarity
Amino acid modifications
	Disulfide bond	430		Interchain Potential

Sequences

	Sequence		Length	Mass (Da)
	P39868-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: BA06FEC14114DC21	FASTA	911	102,201
	10 20 30 40 50 60 MATSVDNRHY PRLNPAINGV VRSFKPPPIP SPRHQNKTVS FLTEKVIVKE TKNDAVDDSY 70 80 90 100 110 120 DSSDDEDESH NRNVSYYKEM IRKSHSDVEP SILDSRDEST ADNWIHRNSS MVRLTGKHPF 130 140 150 160 170 180 NAEPPLPRLM HHGFITPVPL HYVRNHGTVP KADWSEWTVE ITGLVKRPAK FTMEELISEF 190 200 210 220 230 240 PSREFPVTLV CAGNRRKEQN MVKQTIGFNW GSAGVSTSLW KGVPLSEILG RCGIYSRRGG 250 260 270 280 290 300 GLNVCFEGAE DLPGGGGSKY GTSIKKEMAM DPARDIILAY MQNGELLTPD HGFPVRIIVP 310 320 330 340 350 360 GFIGGRMVKW LKRIIVTPQE SDSYYHYKDN RVLPSLVDAE LANAEAWWYK PEYIINELNI 370 380 390 400 410 420 NSVITTPGHQ EILPINAFTT QKPYTLKGYA YSGGGKKVTR VEVTLDGGDT WSVCDLDHQE 430 440 450 460 470 480 KPNKYGKFWC WCFWSLDVEV LDLLSAKDVA VRAWDESFNT QPDKLIWNLM GMMNNCWFRI 490 500 510 520 530 540 KTNVCKPHKG EIGIVFEHPT RPGNQSGGWM AKERQLEISS ESNPILKKSV SSPFMNTSAK 550 560 570 580 590 600 MYSMSEVRKH NSVESAWIIV HGHIYDCTRF LKDHPGGSDS ILINAGTDCT EEFEAIHSDK 610 620 630 640 650 660 AKKLLEDYRI GELITTGYDS SPNVSVHGGS TAVSLLAPIK ELAPSKNIAL VNPREKVPVT 670 680 690 700 710 720 LIEKTSISHD VRRFRFALPS EDQQLGLPVG KHIFLCANIN DKLCLRAYTP TSTVDAVGHI 730 740 750 760 770 780 DLVVKVYFKD VHPRFPNGGL MSQHLDSLPI GSVLNIKGPL GHIEYLGKGN FMVTGKPKFA 790 800 810 820 830 840 KKLAMLAGGP GITPIYQVIQ SILSDPEDET EMFVVYANRT EDDILVREEL EGWANKHKDR 850 860 870 880 890 900 LKVWYVVEIA KEGWNYSTGF ITEPVLREHV PEGLEGESLA LACGPPPMIQ FALQPNLEKM 910 GYNVKEDLLI F

References

[1]	"Developmental stage-specific and nitrate-independent regulation of nitrate reductase gene expression in rapeseed." Fukuoka H., Ogawa T., Minami H., Yano H., Ohkawa Y. Plant Physiol. 111:39-47(1996) [PubMed: 8685274] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Lisandra.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D38220 mRNA. Translation: BAA07395.1.
PIR	T08108.
3D structure databases
HSSP	HSSP built from PDB template 2CND based on UniProtKB P17571.
ModBase	Search...
Family and domain databases
InterPro	IPR001199. Cyt_B5. IPR001834. Cyt_B5_reductase. IPR001709. FPN_cyt_redctse. IPR005066. MoCF_OxRdtse_dimer. IPR008335. Mopterin_OxRdtase_euk. IPR012137. Nitr_rd_NADH. IPR008333. OxRdtase_FAD-bd. IPR001433. OxRdtase_FAD/NAD_bd. IPR000572. OxRdtase_Mopterin-bd. [Graphical view]
Gene3D	G3DSA:3.10.120.10. Cyt_B5. 1 hit. G3DSA:2.60.40.650. MoCF_oxrdtse_dimer. 1 hit. G3DSA:3.90.420.10. Oxred_molyb_bd. 1 hit.
Pfam	PF00173. Cyt-b5. 1 hit. PF00970. FAD_binding_6. 1 hit. PF03404. Mo-co_dimer. 1 hit. PF00175. NAD_binding_1. 1 hit. PF00174. Oxidored_molyb. 1 hit. [Graphical view]
PIRSF	PIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTS	PR00406. CYTB5RDTASE. PR00363. CYTOCHROMEB5. PR00407. EUMOPTERIN. PR00371. FPNCR.
ProDom	PD000612. Cyt_B5. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00191. CYTOCHROME_B5_1. 1 hit. PS50255. CYTOCHROME_B5_2. 1 hit. PS51384. FAD_FR. 1 hit. PS00559. MOLYBDOPTERIN_EUK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NIA2_BRANA

Accession

Primary (citable) accession number: P39868

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 25, 2008
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)