Reviewed, UniProtKB/Swiss-Prot P39867 (NIA1_BRANA)

Names and origin

Protein names

Recommended name:
    Nitrate reductase [NADH], clone PBNBR1405
      Short name=NR
    EC=1.7.1.1

Gene names

Name:

NIA1

Organism

Brassica napus (Rape)

Taxonomic identifier

3708 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Brassica

Protein attributes

Sequence length	911 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.
Catalytic activity	Nitrite + NAD+ + H2O = nitrate + NADH.
Cofactor	Binds 1 FAD. Binds 1 heme group. The heme group is called cytochrome b-557. Binds 1 molybdenum ion.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the nitrate reductase family. Contains 1 cytochrome b5 heme-binding domain. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Biological process	Nitrate assimilation
Ligand	FAD Flavoprotein Heme Iron Metal-binding Molybdenum NAD
Molecular function	Oxidoreductase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	nitrate assimilation Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitrate reductase (NADH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 911	911	Nitrate reductase [NADH], clone PBNBR1405		PRO_0000166051
Regions
	Domain	539 – 614	76	Cytochrome b5 heme-binding
	Domain	654 – 766	113	FAD-binding FR-type
Sites
	Metal binding	191	1	Molybdenum-pterin Potential
	Metal binding	245	1	Molybdenum-pterin Potential
	Metal binding	574	1	Iron (heme axial ligand) By similarity
	Metal binding	597	1	Iron (heme axial ligand) By similarity
Amino acid modifications
	Disulfide bond	430		Interchain Potential

Sequences

	Sequence		Length	Mass (Da)
	P39867-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: BBA4E19835B03D8C	FASTA	911	102,252
	10 20 30 40 50 60 MATSVDNRHY PRLSSALNGG VVHSFKPPLV PSPSLDRDQD QSVNVPTEKS VDKTTKEDRF 70 80 90 100 110 120 DSSDDEDESH NRYVSYYKEM VLKSNSDLEP TALDSRDEST GDKWIHRNSS MVRLTGKHPF 130 140 150 160 170 180 NAEAPLPRLM HHGFITPVPL HYVRNHGGVP KAEWSDWSVE VTGLVKRPAG LTMEQLISEF 190 200 210 220 230 240 PSREFPVTLV CAGNRRKEQN MVKQTIGFNW GSAGVSTSLW RGVALSDVLR RCGVYSKRGG 250 260 270 280 290 300 ALNVCFEGAE DLPGGGGSKY GTSIKKEMAM DPARDIILAY MQNGELLTPD HGFPVRVIIP 310 320 330 340 350 360 GFIGGRMVKW LKRIIVTPQE SDNYYHYKDN RVLPSYVDAE LPNEESWWYR PEYIINELNI 370 380 390 400 410 420 NSVITTPGHE EILPINAFTT QKPYTLKGYA YSGGGKKVTR VEVTLDGGET WSVCELDHQE 430 440 450 460 470 480 KPNKYGKFWC WCFWSLDVEV LDLLSAKEVA VRAWDESLNT QPEKLIWNLM GMMNNCWFRI 490 500 510 520 530 540 KTNVCKPHRG EIGIVFEHPT RPGNQSGGWM AKERQIEKSS ESHPTLKKSV STPFMNTASK 550 560 570 580 590 600 MYSMSEVRKH NSAESAWIIV HGHIYDCTRF LKDHPGGSDS ILINAGTDCT EEFEAIHSDK 610 620 630 640 650 660 AKKLLEDYRI GELITTGYDS SPNVSVHGGS SVMSLLAPIR QLAPTKNIAL VNPREKVPVK 670 680 690 700 710 720 LIEKTSISHD VRRFRFALPS EDQQLGLPVG KHIFLCATIN DKLCLRAYTP TSTVDAVGYI 730 740 750 760 770 780 DLVIKVYFKN VHPRFPNGGL MSQHPDSLPI GAVLDIKGPL GHIEYQGRGK FMVSGKPKFA 790 800 810 820 830 840 NKLAMLAGGT GITPIYQVIQ SILSDPEDET EMFVVYANRT EDDILVREEL EGWASKFPDR 850 860 870 880 890 900 LKIWYVVEIA KEGWEYSTGF ITEAVLREHV PEGLEGESLA LACGPPPMIQ FALQPNLEKM 910 GYDIKEDLLI F

References

[1]	"Developmental stage-specific and nitrate-independent regulation of nitrate reductase gene expression in rapeseed." Fukuoka H., Ogawa T., Minami H., Yano H., Ohkawa Y. Plant Physiol. 111:39-47(1996) [PubMed: 8685274] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Lisandra.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D38219 mRNA. Translation: BAA07394.1.
PIR	T08105.
3D structure databases
HSSP	HSSP built from PDB template 2CND based on UniProtKB P17571.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.7.1.1. 393.
Family and domain databases
InterPro	IPR001199. Cyt_B5. IPR018506. Cyt_B5_heme-BS. IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR005066. MoCF_OxRdtse_dimer. IPR008335. Mopterin_OxRdtase_euk. IPR001834. NADH-Cyt_B5_reductase. IPR012137. Nitr_rd_NADH. IPR008333. OxRdtase_FAD-bd. IPR001433. OxRdtase_FAD/NAD_bd. IPR000572. OxRdtase_Mopterin-bd. [Graphical view]
Gene3D	G3DSA:3.10.120.10. Cyt_B5. 1 hit. G3DSA:2.60.40.650. MoCF_oxrdtse_dimer. 1 hit. G3DSA:3.90.420.10. Oxred_molyb_bd. 1 hit.
Pfam	PF00173. Cyt-b5. 1 hit. PF00970. FAD_binding_6. 1 hit. PF03404. Mo-co_dimer. 1 hit. PF00175. NAD_binding_1. 1 hit. PF00174. Oxidored_molyb. 1 hit. [Graphical view]
PIRSF	PIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTS	PR00406. CYTB5RDTASE. PR00363. CYTOCHROMEB5. PR00407. EUMOPTERIN. PR00371. FPNCR.
ProDom	PD000612. Cyt_B5. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00191. CYTOCHROME_B5_1. 1 hit. PS50255. CYTOCHROME_B5_2. 1 hit. PS51384. FAD_FR. 1 hit. PS00559. MOLYBDOPTERIN_EUK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NIA1_BRANA

Accession

Primary (citable) accession number: P39867

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	June 16, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)