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Protein

Nitrate reductase [NADH] 2

Gene

NIA2

Organism
Phaseolus vulgaris (Kidney bean) (French bean)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.

Catalytic activityi

Nitrite + NAD+ + H2O = nitrate + NADH.

Cofactori

Protein has several cofactor binding sites:
  • FADBy similarityNote: Binds 1 FAD.By similarity
  • hemeBy similarityNote: Binds 1 heme group. The heme group is called cytochrome b-557.By similarity
  • Mo-molybdopterinBy similarityNote: Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi165MolybdenumBy similarity1
Metal bindingi548Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi571Iron (heme axial ligand)PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processNitrate assimilation
LigandFAD, Flavoprotein, Heme, Iron, Metal-binding, Molybdenum, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate reductase [NADH] 2 (EC:1.7.1.1)
Short name:
NR-2
Gene namesi
Name:NIA2
Synonyms:NR2
OrganismiPhaseolus vulgaris (Kidney bean) (French bean)
Taxonomic identifieri3885 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaePhaseolus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001660681 – 890Nitrate reductase [NADH] 2Add BLAST890

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi404InterchainSequence analysis

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP39866

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP39866
SMRiP39866
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini513 – 588Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd BLAST76
Domaini634 – 746FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST113

Sequence similaritiesi

Belongs to the nitrate reductase family.Curated

Family and domain databases

Gene3Di3.10.120.10, 1 hit
3.90.420.10, 1 hit
InterProiView protein in InterPro
IPR001199 Cyt_B5-like_heme/steroid-bd
IPR036400 Cyt_B5-like_heme/steroid_sf
IPR018506 Cyt_B5_heme-BS
IPR017927 Fd_Rdtase_FAD-bd
IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR014756 Ig_E-set
IPR005066 MoCF_OxRdtse_dimer
IPR008335 Mopterin_OxRdtase_euk
IPR001834 NADH-Cyt_B5_reductase
IPR012137 Nitr_rd_NADH
IPR008333 OxRdtase_FAD-bd_dom
IPR001433 OxRdtase_FAD/NAD-bd
IPR000572 OxRdtase_Mopterin-bd_dom
IPR036374 OxRdtase_Mopterin-bd_sf
IPR022407 OxRdtase_Mopterin_BS
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00173 Cyt-b5, 1 hit
PF00970 FAD_binding_6, 1 hit
PF03404 Mo-co_dimer, 1 hit
PF00175 NAD_binding_1, 1 hit
PF00174 Oxidored_molyb, 1 hit
PIRSFiPIRSF000233 Nitr_rd_NADH, 1 hit
PRINTSiPR00406 CYTB5RDTASE
PR00363 CYTOCHROMEB5
PR00407 EUMOPTERIN
PR00371 FPNCR
SMARTiView protein in SMART
SM01117 Cyt-b5, 1 hit
SUPFAMiSSF55856 SSF55856, 1 hit
SSF56524 SSF56524, 1 hit
SSF63380 SSF63380, 1 hit
SSF81296 SSF81296, 1 hit
PROSITEiView protein in PROSITE
PS00191 CYTOCHROME_B5_1, 1 hit
PS50255 CYTOCHROME_B5_2, 1 hit
PS51384 FAD_FR, 1 hit
PS00559 MOLYBDOPTERIN_EUK, 1 hit

Sequencei

Sequence statusi: Complete.

P39866-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASVGNRQF ATHMNGVVRS CGQDLKPSLP LDFDLDSSSS DDDENDDASY
60 70 80 90 100
LKELVRKANA ETEASVMDPR DEGTADQWVA RNASMVRLTG KHPFNAESPL
110 120 130 140 150
QRLMHHGFIT PVPLHYVRNH GPVPKANWED WTVEITGLVK RPTRFTMDRL
160 170 180 190 200
VREFPHREFP ATLVCAGNRR KEQNMVKKTI GFNWGSAGTS TSVWRGVPVR
210 220 230 240 250
HVLRRCGILT RGKGALHVSF EGAENLPGGG GSKYGTSISR EMAMDPSRDI
260 270 280 290 300
ILAYMQNGEP LAPDHGFPIR MIIPGFIGGR MVKWLKRIVV TEQECESHYH
310 320 330 340 350
YKDNRVLPSH VDPELANEEG WWFKPEYIIN ELNINSVITT PCHDEILPIN
360 370 380 390 400
SWTTQRPYVV RGYAYSGGGR KVTRVEVTLD GGETWHVCTL DHPEKPNKYG
410 420 430 440 450
KYWCWCFWSL EVEVLDLLGT KEIAVRAWDE GLNTQPENLI WNLMGMMNNC
460 470 480 490 500
WFRVKTNVCK PHKGEIGIVF EHPTQPGNQP GGWMAKEKHL EQSQEAKPSL
510 520 530 540 550
KKSVSTPFMN TASKMFSVSE VKKHSSPDSA WIIVHGHVYD CTRFLKDHPG
560 570 580 590 600
GTDSILINAG TDCTEEFDAI HSDKAKKMLE DYRIGELITT GYTSADSSPN
610 620 630 640 650
NSVHGNSEFI HLAPINEITT IPPLPPRSVA LNPRQKIPCK LVSKTSISHD
660 670 680 690 700
VRLFRFEMPS KNQLLGLPVG KHIFLCATID GKLCMRAYTP TSSVEEVGFF
710 720 730 740 750
DLLIKVYFKD VHPKFPNGGL MSQYLESLSI GSMLDVKGPL GHIEYTGRGN
760 770 780 790 800
FTVNGKSRFA KRLAMLAGGT GITPIYQVAQ AILKDPEDLT EMHVVYANRT
810 820 830 840 850
EDDILLREEL DTWAKEHCER FKVWYVVETA KEGWGYGVGF ITEAIMREHL
860 870 880 890
PEASSDSLAM TCGPPPMIQF AVQPNLEKMG YDIKNDLLVF
Length:890
Mass (Da):99,995
Last modified:February 1, 1995 - v1
Checksum:iFC03B96F0139DE1E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01029 Unassigned DNA Translation: AAA95940.1
PIRiT11805

Similar proteinsi

Entry informationi

Entry nameiNIA2_PHAVU
AccessioniPrimary (citable) accession number: P39866
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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