Reviewed, UniProtKB/Swiss-Prot P39865 (NIA1_PHAVU)

Names and origin

Protein names

Recommended name:
    Nitrate reductase [NADH] 1
      Short name=NR-1
    EC=1.7.1.1

Gene names

Name:	NIA1
Synonyms:	NR1

Organism

Phaseolus vulgaris (Kidney bean) (French bean)

Taxonomic identifier

3885 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Phaseolus

Protein attributes

Sequence length	881 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.
Catalytic activity	Nitrite + NAD(+) + H(2)O = nitrate + NADH.
Cofactor	Binds 1 FAD. Binds 1 heme group. The heme group is called cytochrome b-557. Binds 1 molybdenum ion.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the nitrate reductase family. Contains 1 cytochrome b5 heme-binding domain. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Biological process	Nitrate assimilation
Ligand	FAD Flavoprotein Heme Iron Metal-binding Molybdenum NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	nitrate assimilation Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro molybdenum ion binding Inferred from electronic annotation. Source: InterPro nitrate reductase (NADH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 881	881	Nitrate reductase [NADH] 1		PRO_0000166067
Regions
	Domain	515 – 590	76	Cytochrome b5 heme-binding
	Domain	625 – 737	113	FAD-binding FR-type
Sites
	Metal binding	167	1	Molybdenum-pterin Potential
	Metal binding	221	1	Molybdenum-pterin Potential
	Metal binding	550	1	Iron (heme axial ligand) By similarity
	Metal binding	573	1	Iron (heme axial ligand) By similarity
Amino acid modifications
	Disulfide bond	406		Interchain Potential

Sequences

	Sequence		Length	Mass (Da)
	P39865-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: A212A1288B4EE661	FASTA	881	99,223
	10 20 30 40 50 60 MAASVEHRPF TSHQHGVVRS FKSYPDVPRP KKLPLPQPLS DSTNDNDEEA AVWKELVLKS 70 80 90 100 110 120 SAGVEPSIFD PRDDGTSDQW IKRNASMIRL TGKHPFNAEP PLPRLMQHGF ITPSPIHYVR 130 140 150 160 170 180 SHGPVPHARW EDWTVEVTGL VTRPTCFTME QLVNDFPSHE FPATLVCAGN RRKEQNMVKQ 190 200 210 220 230 240 SIGFNWGAGG VSTSVWRGVS LRSLLKRCGI YSRAKGALHV CFEGAEDLPG GGGSNYGTSL 250 260 270 280 290 300 MREVALDPSR DIILAYMQNG ELLSPDHGFP VRMIIPGFIG GRMVKWLKRI VVSNQQSQSH 310 320 330 340 350 360 YHYKDNKLFP SHVDAELANE EDWWYKPEYI INEVNINSVI TTPSHQEILP INSWTTQMPY 370 380 390 400 410 420 SMRGYAYSGG GRKVTRVEVT LDGGETWQVC SVERLEKPNK YGKYWCWCFW SLEVEVLDIL 430 440 450 460 470 480 GAKEIAVRAW DEAQNTQPEK LIWNTMGMIN NCWFRVKTNV CKPKKGEIGI VFEHPTQPGN 490 500 510 520 530 540 QSGGWMAREK QLEKSSESNP ILKKSVSSPF MNTATKSYSL SEVRRHNNRD SAWIIVNGHV 550 560 570 580 590 600 YDCTRFLKDH PGGEDSILLN AGTDCTEEFE AIHSDKAKKM LEDYRIGELM TTDYTSDSSS 610 620 630 640 650 660 SNNSVHGNSE TTHLAPIREV ALNPREKIPC KLLSKTSISH DVRLLRFALP AEDQVMGLPV 670 680 690 700 710 720 GNHVFLCATV DEKLCMRAYT PTSSVDEVGF FDLVVKVYFK GVHPNFPNGG IMSQHLDSLP 730 740 750 760 770 780 IGSVVDVKGP LGHIEYTGRG NFLVHGKPRF AKRLTMLAGG TGITPIYQVV QAILKDPEDR 790 800 810 820 830 840 TEMYVVYANR TEDDILLKEE LDEWAKKHDR LKVWYVLQAN IREGWEYSVG FITESILREH 850 860 870 880 VPLASPDTLA LTCGPPPMIQ FAVQPNLEKL GYDIQNDLLV F

References

[1]	"Cloning and expression of a gene encoding a root specific nitrate reductase in bean (Phaseolus vulgaris)." Hoff T., Stummann B.M., Henningsen K.W. Physiol. Plantarum 82:197-204(1991) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Saxa. Tissue: Shoot.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X53603 Genomic DNA. Translation: CAA37672.1.
PIR	S25445.
3D structure databases
HSSP	HSSP built from PDB template 2CND based on UniProtKB P17571.
ModBase	Search...
Family and domain databases
InterPro	IPR001199. Cyt_B5. IPR001834. Cyt_B5_reductase. IPR001709. FPN_cyt_redctse. IPR005066. MoCF_OxRdtse_dimer. IPR008335. Mopterin_OxRdtase_euk. IPR012137. Nitr_rd_NADH. IPR008333. OxRdtase_FAD-bd. IPR001433. OxRdtase_FAD/NAD_bd. IPR000572. OxRdtase_Mopterin-bd. [Graphical view]
Gene3D	G3DSA:3.10.120.10. Cyt_B5. 1 hit. G3DSA:2.60.40.650. MoCF_oxrdtse_dimer. 1 hit. G3DSA:3.90.420.10. Oxred_molyb_bd. 1 hit.
Pfam	PF00173. Cyt-b5. 1 hit. PF00970. FAD_binding_6. 1 hit. PF03404. Mo-co_dimer. 1 hit. PF00175. NAD_binding_1. 1 hit. PF00174. Oxidored_molyb. 1 hit. [Graphical view]
PIRSF	PIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTS	PR00406. CYTB5RDTASE. PR00363. CYTOCHROMEB5. PR00407. EUMOPTERIN. PR00371. FPNCR.
ProDom	PD000612. Cyt_B5. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00191. CYTOCHROME_B5_1. 1 hit. PS50255. CYTOCHROME_B5_2. 1 hit. PS51384. FAD_FR. 1 hit. PS00559. MOLYBDOPTERIN_EUK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NIA1_PHAVU

Accession

Primary (citable) accession number: P39865

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 25, 2008
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)