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Protein

Nitrate reductase [NADPH]

Gene

NIAA

Organism
Phytophthora infestans (Potato late blight fungus) (Botrytis infestans)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.

Catalytic activityi

Nitrite + NADP+ + H2O = nitrate + NADPH.

Cofactori

Protein has several cofactor binding sites:
  • FADBy similarityNote: Binds 1 FAD.By similarity
  • hemeBy similarityNote: Binds 1 heme group. The heme group is called cytochrome b-557.By similarity
  • Mo-molybdopterinBy similarityNote: Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi182 – 1821MolybdenumBy similarity
Metal bindingi572 – 5721Iron (heme axial ligand)PROSITE-ProRule annotation
Metal bindingi595 – 5951Iron (heme axial ligand)PROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi872 – 8798NADPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Nitrate assimilation

Keywords - Ligandi

FAD, Flavoprotein, Heme, Iron, Metal-binding, Molybdenum, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate reductase [NADPH] (EC:1.7.1.3)
Short name:
NR
Gene namesi
Name:NIAA
OrganismiPhytophthora infestans (Potato late blight fungus) (Botrytis infestans)
Taxonomic identifieri4787 [NCBI]
Taxonomic lineageiEukaryotaStramenopilesOomycetesPeronosporalesPhytophthora

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 902902Nitrate reductase [NADPH]PRO_0000166047Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi419 – 419InterchainSequence analysis

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi4787.PITG_13012T0.

Structurei

3D structure databases

ProteinModelPortaliP39864.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini537 – 61276Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini637 – 751115FAD-binding FR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the nitrate reductase family.Curated
Contains 1 cytochrome b5 heme-binding domain.PROSITE-ProRule annotation
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0534. Eukaryota.
KOG0535. Eukaryota.
KOG0537. Eukaryota.
COG0543. LUCA.
COG2041. LUCA.
COG5274. LUCA.
HOGENOMiHOG000252609.

Family and domain databases

Gene3Di2.60.40.650. 1 hit.
3.10.120.10. 1 hit.
3.90.420.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR014756. Ig_E-set.
IPR005066. MoCF_OxRdtse_dimer.
IPR008335. Mopterin_OxRdtase_euk.
IPR001834. NADH-Cyt_B5_reductase.
IPR012137. Nitr_rd_NADH.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR000572. OxRdtase_Mopterin-bd_dom.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
PF00970. FAD_binding_6. 1 hit.
PF03404. Mo-co_dimer. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF00174. Oxidored_molyb. 1 hit.
[Graphical view]
PIRSFiPIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTSiPR00406. CYTB5RDTASE.
PR00363. CYTOCHROMEB5.
PR00407. EUMOPTERIN.
PR00371. FPNCR.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
SSF56524. SSF56524. 1 hit.
SSF63380. SSF63380. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
PS51384. FAD_FR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39864-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRLRSAAST ITNRSTPALA LTASHAGSHP AVIVPIQPLL LTLERRPSCP
60 70 80 90 100
HRVSYPEIEA YRGISSSLRS HNAIVRASDI MAQIDPRDVG TPDEWVPRHP
110 120 130 140 150
ELIRLTGRHP FNSEPPLKYA STFITPMALH YVRNHGPVPR LEWDTHTFSI
160 170 180 190 200
DGLVKKPRTF GMNELVTTFE QETVTFPVLL VCAGNRRKEQ NMIKKTIGFS
210 220 230 240 250
WGAAGCSTAE WTGVPLHVLL TACGVDREKA QWVWFEGIED LPHDKYGTCI
260 270 280 290 300
RASTELDPEC DVLVAWKANG ELLGPDHGFP VRLIVPGHIG GRMVKWLERI
310 320 330 340 350
HVSDHESSNH HHIMDNRVLP SHVTAETATA EGWWSKSPYA IMELNVNAVV
360 370 380 390 400
ILPNHDDLLA LGEDTTFNDI ETYTIKGYAY SGGGRRVIRV EVTLDDGASW
410 420 430 440 450
QLARIIYHER PSKYGKMWCW VHYELAAPMS SLLCAREVCV RAWDSSMNLM
460 470 480 490 500
PAFPTWNVMG MMNNPWYRVK IHHEQDTNSL RFEHPTQAGN QKGGWMTKER
510 520 530 540 550
IMTNDVDSIK MLQVEPLDTS SAATPKPGLT ADELSELPLI FADEVAKHNS
560 570 580 590 600
KKSCWFICRD LVYDATPFLD EHPGGATSIL LCGGTDCTDE FESIHSTKAW
610 620 630 640 650
QMLKKYCIGR CSSTEDDTGT SDTSSDHEET DVALKGRTKV PIVLISREVV
660 670 680 690 700
SHDARIFKFA LPAKDLRLGL PIGNHVFLYA KINGKTAVRA YTPISSENDE
710 720 730 740 750
DRGFVSFLIK VYFAGDNPVH PEGGLFSQYL DGLHLGQQIQ IKGPLGHFTY
760 770 780 790 800
YGDGNFSLET TNFHAYKFGF VAGGTGITPV YQVMRAILED AKDQTKVALI
810 820 830 840 850
YCVRSQRDLL LRKELETLQK LRPGQCRIFY TLSDMELLDR NDPIVRGWAY
860 870 880 890 900
GKSRLNFAMV KNIIGSDAED VCMCGPEGMI EYACKPALLK LNYDLKTQTT

VF
Length:902
Mass (Da):101,074
Last modified:February 1, 1995 - v1
Checksum:i05B9F4E1A381BBE6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14405 Genomic DNA. Translation: AAA86681.1.
PIRiS57199.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14405 Genomic DNA. Translation: AAA86681.1.
PIRiS57199.

3D structure databases

ProteinModelPortaliP39864.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4787.PITG_13012T0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG0534. Eukaryota.
KOG0535. Eukaryota.
KOG0537. Eukaryota.
COG0543. LUCA.
COG2041. LUCA.
COG5274. LUCA.
HOGENOMiHOG000252609.

Family and domain databases

Gene3Di2.60.40.650. 1 hit.
3.10.120.10. 1 hit.
3.90.420.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR014756. Ig_E-set.
IPR005066. MoCF_OxRdtse_dimer.
IPR008335. Mopterin_OxRdtase_euk.
IPR001834. NADH-Cyt_B5_reductase.
IPR012137. Nitr_rd_NADH.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR000572. OxRdtase_Mopterin-bd_dom.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
PF00970. FAD_binding_6. 1 hit.
PF03404. Mo-co_dimer. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF00174. Oxidored_molyb. 1 hit.
[Graphical view]
PIRSFiPIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTSiPR00406. CYTB5RDTASE.
PR00363. CYTOCHROMEB5.
PR00407. EUMOPTERIN.
PR00371. FPNCR.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
SSF56524. SSF56524. 1 hit.
SSF63380. SSF63380. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
PS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNIA_PHYIN
AccessioniPrimary (citable) accession number: P39864
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 11, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.