Reviewed, UniProtKB/Swiss-Prot P39863 (NIA_FUSOX)

Names and origin

Protein names

Recommended name:
    Nitrate reductase [NADPH]
      Short name=NR
    EC=1.7.1.3

Gene names

Name:

NIA

Organism

Fusarium oxysporum

Taxonomic identifier

5507 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Hypocreomycetidae › Hypocreales › mitosporic Hypocreales › Fusarium › Fusarium oxysporum species complex

Protein attributes

Sequence length	905 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.
Catalytic activity	Nitrite + NADP+ + H2O = nitrate + NADPH.
Cofactor	Binds 1 FAD. Binds 1 heme group. The heme group is called cytochrome b-557. Binds 1 molybdenum ion.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the nitrate reductase family. Contains 1 cytochrome b5 heme-binding domain. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Biological process	Nitrate assimilation
Ligand	FAD Flavoprotein Heme Iron Metal-binding Molybdenum NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	nitrate assimilation Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitrate reductase (NADPH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 905	905	Nitrate reductase [NADPH]		PRO_0000166043
Regions
	Domain	546 – 621	76	Cytochrome b5 heme-binding
	Domain	648 – 759	112	FAD-binding FR-type
	Nucleotide binding	875 – 884	10	NADP By similarity
	Compositional bias	3 – 8	6	Poly-Thr
Sites
	Metal binding	179	1	Molybdenum-pterin Potential
	Metal binding	230	1	Molybdenum-pterin Potential
	Metal binding	581	1	Iron (heme axial ligand) By similarity
	Metal binding	604	1	Iron (heme axial ligand) By similarity
Amino acid modifications
	Disulfide bond	428		Interchain Potential

Sequences

	Sequence		Length	Mass (Da)
	P39863-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: D5D8E23F7971ACDA	FASTA	905	101,899
	10 20 30 40 50 60 METSTTTTLL QQERIPENSE PISTHIHTHS LPPTPPGTAK PSRIGSFHDL QELSSVESPR 70 80 90 100 110 120 LDHIKPYPLP PKFSPKSVLK EDLKTPDNFV ERDPRLIRLT GVHPFNVEAP LSDLYDEGFL 130 140 150 160 170 180 TSENLHYVRN HGHVPRCEDD DILDWEFEIS GLVENPIKMN VRDLINDYQQ LTYPVTFVCA 190 200 210 220 230 240 GNRRKEQNIV RKTKGFSWGP AGLSTALWTG VAIGDLLSAA KPKRGARYVC FEGADKLPNG 250 260 270 280 290 300 YYGTSIKLNW CMDPNRGVMV AHKMNGNPLP PDHGKPVRIV IPGQIGGRSI KWLKKITITQ 310 320 330 340 350 360 EPSDNWYHIY DNRVLPTMIS PEESANLPEV WKDEKYAIYD LSTNSAICYP AHEEKVPFTD 370 380 390 400 410 420 APASYKVRGY AYSGGGRRIT RVEVTLDKGK SWRLANIRYP EDDYRNAPEG DTLYGGRVDM 430 440 450 460 470 480 WWRETSFCWC FWDLDIPLDE LKSADDIMMR AMDESMNVQP RDMYWSVLGM MNNPWFRIVI 490 500 510 520 530 540 HKEDHALRFE HPTHATLKIK GWMERVKEAG GDLTNGYWGE KAPGEVQEVV VKEPEKQICM 550 560 570 580 590 600 TNPQINRKIT IEELKAHSGE EEPWFVVKGE VYDGTPYLSG HPGGAASIFG AAGQDATEEF 610 620 630 640 650 660 MAIHSENAKA MLPTYHIGTL DEESRAILSG DATKTNDDAD REVFLQAKTW SKAILDKKTS 670 680 690 700 710 720 ISPDTKIFSF KLNHEAQKIG LPTGQHLMMR LRDPATREAI IRSYTPYSDG SDCGRLDILI 730 740 750 760 770 780 KIYYDTPQRK GGVMTQALDA LPIGHWVDFK GPTGKFVYHG NGLCTINEKE RRVRRFIMVC 790 800 810 820 830 840 GGSGITPIRQ VLRAVIDNPK DTTPCLVFNG NRSVNDILCM EELEELEAAN PSRCRVVNAL 850 860 870 880 890 900 SNPPPEWNGL KGFVNQALVP EYMDLPKASG EGDELLLVCG PPPMVKAVEA SFLGMGFKSD DFVFF

References

[1]	"The nia gene of Fusarium oxysporum: isolation, sequence and development of a homologous transformation system." Diolez A., Langin T., Gerlinger C., Brygoo Y., Daboussi M.-J. Gene 131:61-67(1993) [PubMed: 8370541] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: FOM24.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z22549 Genomic DNA. Translation: CAA80270.1.
PIR	JN0803.
3D structure databases
HSSP	HSSP built from PDB template 1EUE based on UniProtKB P04166.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.7.1.3. 15244.
Family and domain databases
InterPro	IPR001199. Cyt_B5. IPR018506. Cyt_B5_heme-BS. IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR005066. MoCF_OxRdtse_dimer. IPR008335. Mopterin_OxRdtase_euk. IPR001834. NADH-Cyt_B5_reductase. IPR012137. Nitr_rd_NADH. IPR008333. OxRdtase_FAD-bd. IPR001433. OxRdtase_FAD/NAD_bd. IPR000572. OxRdtase_Mopterin-bd. [Graphical view]
Gene3D	G3DSA:3.10.120.10. Cyt_B5. 1 hit. G3DSA:2.60.40.650. MoCF_oxrdtse_dimer. 1 hit. G3DSA:3.90.420.10. Oxred_molyb_bd. 1 hit.
Pfam	PF00173. Cyt-b5. 1 hit. PF00970. FAD_binding_6. 1 hit. PF03404. Mo-co_dimer. 1 hit. PF00175. NAD_binding_1. 1 hit. PF00174. Oxidored_molyb. 1 hit. [Graphical view]
PIRSF	PIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTS	PR00406. CYTB5RDTASE. PR00363. CYTOCHROMEB5. PR00407. EUMOPTERIN. PR00371. FPNCR.
ProDom	PD000612. Cyt_B5. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00191. CYTOCHROME_B5_1. 1 hit. PS50255. CYTOCHROME_B5_2. 1 hit. PS51384. FAD_FR. 1 hit. PS00559. MOLYBDOPTERIN_EUK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NIA_FUSOX

Accession

Primary (citable) accession number: P39863

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	June 16, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)