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Protein

Nitrate reductase [NADPH]

Gene

NIA

Organism
Fusarium oxysporum (Fusarium vascular wilt)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.

Catalytic activityi

Nitrite + NADP+ + H2O = nitrate + NADPH.

Cofactori

Protein has several cofactor binding sites:
  • FADBy similarityNote: Binds 1 FAD.By similarity
  • hemeBy similarityNote: Binds 1 heme group. The heme group is called cytochrome b-557.By similarity
  • Mo-molybdopterinBy similarityNote: Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi179 – 1791MolybdenumBy similarity
Metal bindingi581 – 5811Iron (heme axial ligand)PROSITE-ProRule annotation
Metal bindingi604 – 6041Iron (heme axial ligand)PROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi875 – 88410NADPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Nitrate assimilation

Keywords - Ligandi

FAD, Flavoprotein, Heme, Iron, Metal-binding, Molybdenum, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate reductase [NADPH] (EC:1.7.1.3)
Short name:
NR
Gene namesi
Name:NIA
OrganismiFusarium oxysporum (Fusarium vascular wilt)
Taxonomic identifieri5507 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesNectriaceaeFusariumFusarium oxysporum species complex

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 905905Nitrate reductase [NADPH]PRO_0000166043Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi428 – 428InterchainSequence analysis

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi5507.FOXG_04181P0.

Structurei

3D structure databases

ProteinModelPortaliP39863.
SMRiP39863. Positions 66-521.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini546 – 62176Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini648 – 759112FAD-binding FR-typePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi3 – 86Poly-Thr

Sequence similaritiesi

Belongs to the nitrate reductase family.Curated
Contains 1 cytochrome b5 heme-binding domain.PROSITE-ProRule annotation
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0534. Eukaryota.
KOG0535. Eukaryota.
KOG0537. Eukaryota.
COG0543. LUCA.
COG2041. LUCA.

Family and domain databases

Gene3Di2.60.40.650. 1 hit.
3.10.120.10. 1 hit.
3.90.420.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR014756. Ig_E-set.
IPR005066. MoCF_OxRdtse_dimer.
IPR008335. Mopterin_OxRdtase_euk.
IPR001834. NADH-Cyt_B5_reductase.
IPR012137. Nitr_rd_NADH.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR000572. OxRdtase_Mopterin-bd_dom.
IPR022407. OxRdtase_Mopterin_BS.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
PF00970. FAD_binding_6. 1 hit.
PF03404. Mo-co_dimer. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF00174. Oxidored_molyb. 1 hit.
[Graphical view]
PIRSFiPIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTSiPR00406. CYTB5RDTASE.
PR00363. CYTOCHROMEB5.
PR00407. EUMOPTERIN.
PR00371. FPNCR.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
SSF56524. SSF56524. 1 hit.
SSF63380. SSF63380. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
PS51384. FAD_FR. 1 hit.
PS00559. MOLYBDOPTERIN_EUK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39863-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METSTTTTLL QQERIPENSE PISTHIHTHS LPPTPPGTAK PSRIGSFHDL
60 70 80 90 100
QELSSVESPR LDHIKPYPLP PKFSPKSVLK EDLKTPDNFV ERDPRLIRLT
110 120 130 140 150
GVHPFNVEAP LSDLYDEGFL TSENLHYVRN HGHVPRCEDD DILDWEFEIS
160 170 180 190 200
GLVENPIKMN VRDLINDYQQ LTYPVTFVCA GNRRKEQNIV RKTKGFSWGP
210 220 230 240 250
AGLSTALWTG VAIGDLLSAA KPKRGARYVC FEGADKLPNG YYGTSIKLNW
260 270 280 290 300
CMDPNRGVMV AHKMNGNPLP PDHGKPVRIV IPGQIGGRSI KWLKKITITQ
310 320 330 340 350
EPSDNWYHIY DNRVLPTMIS PEESANLPEV WKDEKYAIYD LSTNSAICYP
360 370 380 390 400
AHEEKVPFTD APASYKVRGY AYSGGGRRIT RVEVTLDKGK SWRLANIRYP
410 420 430 440 450
EDDYRNAPEG DTLYGGRVDM WWRETSFCWC FWDLDIPLDE LKSADDIMMR
460 470 480 490 500
AMDESMNVQP RDMYWSVLGM MNNPWFRIVI HKEDHALRFE HPTHATLKIK
510 520 530 540 550
GWMERVKEAG GDLTNGYWGE KAPGEVQEVV VKEPEKQICM TNPQINRKIT
560 570 580 590 600
IEELKAHSGE EEPWFVVKGE VYDGTPYLSG HPGGAASIFG AAGQDATEEF
610 620 630 640 650
MAIHSENAKA MLPTYHIGTL DEESRAILSG DATKTNDDAD REVFLQAKTW
660 670 680 690 700
SKAILDKKTS ISPDTKIFSF KLNHEAQKIG LPTGQHLMMR LRDPATREAI
710 720 730 740 750
IRSYTPYSDG SDCGRLDILI KIYYDTPQRK GGVMTQALDA LPIGHWVDFK
760 770 780 790 800
GPTGKFVYHG NGLCTINEKE RRVRRFIMVC GGSGITPIRQ VLRAVIDNPK
810 820 830 840 850
DTTPCLVFNG NRSVNDILCM EELEELEAAN PSRCRVVNAL SNPPPEWNGL
860 870 880 890 900
KGFVNQALVP EYMDLPKASG EGDELLLVCG PPPMVKAVEA SFLGMGFKSD

DFVFF
Length:905
Mass (Da):101,899
Last modified:February 1, 1995 - v1
Checksum:iD5D8E23F7971ACDA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22549 Genomic DNA. Translation: CAA80270.1.
PIRiJN0803.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22549 Genomic DNA. Translation: CAA80270.1.
PIRiJN0803.

3D structure databases

ProteinModelPortaliP39863.
SMRiP39863. Positions 66-521.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5507.FOXG_04181P0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG0534. Eukaryota.
KOG0535. Eukaryota.
KOG0537. Eukaryota.
COG0543. LUCA.
COG2041. LUCA.

Family and domain databases

Gene3Di2.60.40.650. 1 hit.
3.10.120.10. 1 hit.
3.90.420.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR014756. Ig_E-set.
IPR005066. MoCF_OxRdtse_dimer.
IPR008335. Mopterin_OxRdtase_euk.
IPR001834. NADH-Cyt_B5_reductase.
IPR012137. Nitr_rd_NADH.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR000572. OxRdtase_Mopterin-bd_dom.
IPR022407. OxRdtase_Mopterin_BS.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
PF00970. FAD_binding_6. 1 hit.
PF03404. Mo-co_dimer. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF00174. Oxidored_molyb. 1 hit.
[Graphical view]
PIRSFiPIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTSiPR00406. CYTB5RDTASE.
PR00363. CYTOCHROMEB5.
PR00407. EUMOPTERIN.
PR00371. FPNCR.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
SSF56524. SSF56524. 1 hit.
SSF63380. SSF63380. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
PS51384. FAD_FR. 1 hit.
PS00559. MOLYBDOPTERIN_EUK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNIA_FUSOX
AccessioniPrimary (citable) accession number: P39863
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 11, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.