Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P39848

- LYTD_BACSU

UniProt

P39848 - LYTD_BACSU

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Beta-N-acetylglucosaminidase

Gene

lytD

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Cell wall hydrolase not involved in cell autolysis, competence, sporulation or germination. It hydrolyzes the beta-1,4 glycan bond between the N-acetylglucosaminyl and the N-acetylmuramoyl residues in the glycan chain.2 Publications

Catalytic activityi

Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)2)Asn-structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact.

Enzyme regulationi

Inhibited by diethyl pyrocarbonate, slightly by EDTA. Not inhibited by PMSF, diisopropyl fluorophosphate, 2-mercaptoethanol or N-ethylmaleimide.1 Publication

GO - Molecular functioni

  1. amidase activity Source: InterPro
  2. mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity Source: UniProtKB-EC

GO - Biological processi

  1. cell wall organization Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciBSUB:BSU35780-MONOMER.

Protein family/group databases

CAZyiGH73. Glycoside Hydrolase Family 73.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-N-acetylglucosaminidase (EC:3.2.1.96)
Alternative name(s):
Cell wall-associated polypeptide 90
Short name:
CWBP90
Gene namesi
Name:lytD
Synonyms:cwlG
Ordered Locus Names:BSU35780
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU35780. [Micado]

Subcellular locationi

Secreted 1 Publication. Secretedcell wall 1 Publication

GO - Cellular componenti

  1. cell wall Source: UniProtKB-KW
  2. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene show no motility changes on swarm plates; however in combination with an amidase deletion (lytC, AC Q02114) greatly reduced motility is seen.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 27271 PublicationAdd
BLAST
Chaini28 – 880853Beta-N-acetylglucosaminidasePRO_0000012118Add
BLAST

Proteomic databases

PaxDbiP39848.

Expressioni

Inductioni

In stationary phase (PubMed:11987133); under control of SigD (PubMed:7581999).2 Publications

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi224308.BSU35780.

Structurei

3D structure databases

ProteinModelPortaliP39848.
SMRiP39848. Positions 632-682, 735-861.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini70 – 14980SPOR 1Add
BLAST
Domaini150 – 22980SPOR 2Add
BLAST
Domaini230 – 31182SPOR 3Add
BLAST
Repeati439 – 473351Add
BLAST
Repeati479 – 513352Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi72 – 754Poly-Thr
Compositional biasi337 – 3404Poly-Lys
Compositional biasi568 – 5714Poly-Ala

Sequence similaritiesi

Belongs to the glycosyl hydrolase 73 family.Curated
Contains 3 SPOR domains.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiCOG4193.
HOGENOMiHOG000009151.
KOiK01227.
OMAiFQFLKLS.
OrthoDBiEOG6TXQWM.

Family and domain databases

InterProiIPR013338. Lysozyme_subfam2_dom.
IPR002901. MGlyc_endo_b_GlcNAc_like_dom.
IPR003646. SH3-like_bac-type.
IPR007730. Sporulation-related_dom.
[Graphical view]
PfamiPF01832. Glucosaminidase. 1 hit.
PF08239. SH3_3. 1 hit.
PF05036. SPOR. 1 hit.
[Graphical view]
SMARTiSM00047. LYZ2. 1 hit.
SM00287. SH3b. 1 hit.
[Graphical view]
PROSITEiPS51724. SPOR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P39848-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKKRLIAPML LSAASLAFFA MSGSAQAAAY TDYSLYKVEP SNTFSTESQA
60 70 80 90 100
SQAVAKLEKD TGWDASYQAS GTTTTYQISA SGIHSESEAK AILSGLAKQT
110 120 130 140 150
SITGTSSPVG SKQPYVTISS GAISGEKQAN TILAKLKQET GVAGAVKAYG
160 170 180 190 200
AAQPYMNVMT SDIADETKVK ALIQSLAKQT GIKSSYQPIT HTVSVTTIQS
210 220 230 240 250
GTIVGDSRAA QIKNAFQKES GLQASLKETV KGQAYYTFTT AAISGEANAK
260 270 280 290 300
TLLQQLKQST GITGSYKSIN QKTTVESYNV QSAYFKGLST VKDAISQIKK
310 320 330 340 350
NTGVSGSYQQ VGKSTSYTVN MKGITKQQLQ KIDTFFKKKK WHYTSSSVKK
360 370 380 390 400
TTTSAAYQIT TAKILGEQQA NKAAAFFAQK KVKAAKTAAG STAENQYQLI
410 420 430 440 450
SEETSDQAKV TKGLNILKKN QLSASAKSVK KQIADTFKIT TESLLDQTKV
460 470 480 490 500
NQALTFFKSN HISVASQKTG QTAASSYQIT TEAIISQEEI DRVLTFFKQN
510 520 530 540 550
HIAVTTSKTG QTAYTQYKIV TTQLSSKTAL NNGLTYLKSK SVTPSYTTKS
560 570 580 590 600
NTLYKISVNE QFTGNDTAAA ASTKLKQLYG WTSSIVKIKN GPQIMKTNYN
610 620 630 640 650
LSLRDMVQKQ MTVSPQTDGA AYVSLTYINT ATSTVTADVL NIRSTPEVSP
660 670 680 690 700
TNVIGQFKKG DKVKVIGQIN GWAKINLGWR NASSDEVVQY VDPNNFSRDS
710 720 730 740 750
KYYFQFLKLS QTAGLSVTEV NQKVLAGKGI LTGRAKAFID AANQYSINEL
760 770 780 790 800
YLISHALLET GNGTSALANG LTYNGKTVYN MYGIGAYDSN PNYYGAKYAY
810 820 830 840 850
EQGWFTPEAA IIGGAKFIGS SYIHNTAYNQ DTLYKMRWSA TATHQYATDI
860 870 880
GWAYKQVNRM YSLYSLLDGY TLYFDVPEYR
Length:880
Mass (Da):95,554
Last modified:February 1, 1995 - v1
Checksum:i2A912A478FCFC1D1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U02562 Genomic DNA. Translation: AAA67857.1.
D45048 Genomic DNA. Translation: BAA08089.1.
AL009126 Genomic DNA. Translation: CAB15595.1.
PIRiS60137.
RefSeqiNP_391459.1. NC_000964.3.
WP_003243594.1. NZ_CM000487.1.

Genome annotation databases

EnsemblBacteriaiCAB15595; CAB15595; BSU35780.
GeneIDi936822.
KEGGibsu:BSU35780.
PATRICi18979172. VBIBacSub10457_3748.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U02562 Genomic DNA. Translation: AAA67857.1 .
D45048 Genomic DNA. Translation: BAA08089.1 .
AL009126 Genomic DNA. Translation: CAB15595.1 .
PIRi S60137.
RefSeqi NP_391459.1. NC_000964.3.
WP_003243594.1. NZ_CM000487.1.

3D structure databases

ProteinModelPortali P39848.
SMRi P39848. Positions 632-682, 735-861.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224308.BSU35780.

Protein family/group databases

CAZyi GH73. Glycoside Hydrolase Family 73.

Proteomic databases

PaxDbi P39848.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB15595 ; CAB15595 ; BSU35780 .
GeneIDi 936822.
KEGGi bsu:BSU35780.
PATRICi 18979172. VBIBacSub10457_3748.

Organism-specific databases

GenoListi BSU35780. [Micado ]

Phylogenomic databases

eggNOGi COG4193.
HOGENOMi HOG000009151.
KOi K01227.
OMAi FQFLKLS.
OrthoDBi EOG6TXQWM.

Enzyme and pathway databases

BioCyci BSUB:BSU35780-MONOMER.

Family and domain databases

InterProi IPR013338. Lysozyme_subfam2_dom.
IPR002901. MGlyc_endo_b_GlcNAc_like_dom.
IPR003646. SH3-like_bac-type.
IPR007730. Sporulation-related_dom.
[Graphical view ]
Pfami PF01832. Glucosaminidase. 1 hit.
PF08239. SH3_3. 1 hit.
PF05036. SPOR. 1 hit.
[Graphical view ]
SMARTi SM00047. LYZ2. 1 hit.
SM00287. SH3b. 1 hit.
[Graphical view ]
PROSITEi PS51724. SPOR. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The gene of the N-acetylglucosaminidase, a Bacillus subtilis 168 cell wall hydrolase not involved in vegetative cell autolysis."
    Margot P., Maueel C., Karamata D.
    Mol. Microbiol. 12:535-545(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: 168.
  2. "Glucosaminidase of Bacillus subtilis: cloning, regulation, primary structure and biochemical characterization."
    Rashid M.H., Mori M., Sekiguchi J.
    Microbiology 141:2391-2404(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, ENZYME REGULATION, REGULATION BY SIGD.
    Strain: 168 / AC327.
  3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  4. "Bacillus subtilis mutant deficient in the major autolytic amidase and glucosaminidase is impaired in motility."
    Rashid M.H., Kuroda A., Sekiguchi J.
    FEMS Microbiol. Lett. 112:135-140(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF N-TERMINUS, DISRUPTION PHENOTYPE.
    Strain: 168 / AC327.
  5. "Stabilization of cell wall proteins in Bacillus subtilis: a proteomic approach."
    Antelmann H., Yamamoto H., Sekiguchi J., Hecker M.
    Proteomics 2:591-602(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INDUCTION, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: 168.

Entry informationi

Entry nameiLYTD_BACSU
AccessioniPrimary (citable) accession number: P39848
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: October 29, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3