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Protein

Plipastatin synthase subunit C

Gene

ppsC

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This protein is a multifunctional enzyme, able to activate and polymerize the amino acids Glu and Ala/Val as part of the biosynthesis of the lipopeptide antibiotic plipastatin. The Ala/Val residue is further epimerized to the D-isomer form. The activation sites for these amino acids consist of individual domains.1 Publication

Cofactori

pantetheine 4'-phosphateCuratedNote: Binds 2 phosphopantetheines covalently.Curated

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase, Transferase

Keywords - Biological processi

Antibiotic biosynthesis

Enzyme and pathway databases

BioCyciBSUB:BSU18320-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Plipastatin synthase subunit C (EC:2.3.1.-)
Alternative name(s):
Peptide synthase 3
Including the following 2 domains:
ATP-dependent glutamate adenylase 2
Short name:
GluA 2
Alternative name(s):
Glutamate activase 2
ATP-dependent alanine/valine adenylase
Short name:
Ala/ValA
Alternative name(s):
Alanine/valine activase
Gene namesi
Name:ppsC
Synonyms:pps3
Ordered Locus Names:BSU18320
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 25552555Plipastatin synthase subunit CPRO_0000193187Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1002 – 10021O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation
Modified residuei2038 – 20381O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation

Keywords - PTMi

Phosphopantetheine, Phosphoprotein

Proteomic databases

PaxDbiP39847.

Interactioni

Protein-protein interaction databases

IntActiP39847. 16 interactions.
STRINGi224308.Bsubs1_010100010096.

Structurei

3D structure databases

ProteinModelPortaliP39847.
SMRiP39847. Positions 443-958, 963-1482, 1485-1995, 1998-2076.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini972 – 103968Acyl carrier 1PROSITE-ProRule annotationAdd
BLAST
Domaini2008 – 207467Acyl carrier 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni7 – 306300Condensation 1Add
BLAST
Regioni491 – 894404Adenylation 1Add
BLAST
Regioni1054 – 1344291Condensation 2Add
BLAST
Regioni1532 – 1927396Adenylation 2Add
BLAST
Regioni2085 – 2548464Epimerization 3Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1148 – 11514Poly-Ala

Sequence similaritiesi

Contains 2 acyl carrier domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105C0W. Bacteria.
COG1020. LUCA.
HOGENOMiHOG000229991.
InParanoidiP39847.
KOiK15666.
OMAiMVQHHIV.
OrthoDBiEOG6XHC08.

Family and domain databases

Gene3Di1.10.1200.10. 2 hits.
InterProiIPR010071. AA_adenyl_domain.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR001242. Condensatn.
IPR010060. NRPS_synth.
IPR020806. PKS_PP-bd.
IPR009081. PP-bd_ACP.
IPR006162. Ppantetheine_attach_site.
[Graphical view]
PfamiPF00501. AMP-binding. 2 hits.
PF13193. AMP-binding_C. 2 hits.
PF00668. Condensation. 3 hits.
PF00550. PP-binding. 2 hits.
[Graphical view]
SMARTiSM00823. PKS_PP. 2 hits.
[Graphical view]
SUPFAMiSSF47336. SSF47336. 2 hits.
TIGRFAMsiTIGR01733. AA-adenyl-dom. 2 hits.
TIGR01720. NRPS-para261. 1 hit.
PROSITEiPS50075. ACP_DOMAIN. 2 hits.
PS00455. AMP_BINDING. 2 hits.
PS00012. PHOSPHOPANTETHEINE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39847-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPQQPEIQDI YPLSFMQEGM LFHSLYDEQS RAYFEQASFT IHGQLDLERF
60 70 80 90 100
QKSMDAVFDR YDIFRTAFIY KNVAKPRQVV LKQRHCPIHI EDISHLNERD
110 120 130 140 150
KEHCTEAFKE QDKSKGFDLQ TDVLMRISIL KWAPDHYVCI WSHHHILMDG
160 170 180 190 200
WCLGIVIKDF LHIYQALGKG QLPDLPPVQP YGTYIKWLMQ QDREEAAEYW
210 220 230 240 250
KKRLQHFEKS TPLPKRTDQI PNGTLQQITF AIPEKETAEL QKIAAASGAT
260 270 280 290 300
LNTVFQALWG IMLQKVNRSS DAVFGSVISG RPSELKDVEN MVGLFINTIP
310 320 330 340 350
IRAQSDSLSF SDLVRRMQKD MNEAEAYSYF PLYDIQAQSA LKQELIDHII
360 370 380 390 400
VFENTPTQQE IEELNQAGSF DFSVKDFEME EVTNYSCSVK VIPGRTLYVR
410 420 430 440 450
IHFQTSAYQP SMMSEIKDYL LHMVSDVISD PSLPVSKMTL LDEDKTRKIV
460 470 480 490 500
SQNNRTVSVS PEAPTLHGLF ERQAAVTPER LAIRFSGGSL TYAELDMYAS
510 520 530 540 550
RLAAHLAARG VTNESIVGVL SERSPDMLIA VLAVLKAGGA YLPLDPAYPK
560 570 580 590 600
ERLSYMLKDS GASLLLTQPG CSAPNFSGET LEVDMTSLEC EEVKRHVSAS
610 620 630 640 650
VSDGSLAYVI YTSGSTGQPK GVAVEHRQAV SFLTGMQHQF RLSEDDIVMV
660 670 680 690 700
KTSFSFDASV WQLFWWALSG ASAYLLPPGW EKDSALIVQA IHQENVTTAH
710 720 730 740 750
FIPAMLNSFL DQAEIERLSD RTSLKRVFAG GEPLAPRTAA RFASVLPQVS
760 770 780 790 800
LIHGYGPTEA TVDAAFYVLD PERDRDRLRI PIGKPVPGAR LYVLDPHLAV
810 820 830 840 850
QPSGVAGELY IAGAGVARGY LNRPALTEER FLEDPFYLGE RMYKTGDVAR
860 870 880 890 900
WLPDGNVEFL GRTDDQVKIR GYRIEPGEIE AALRSIEGVR EAAVTVRTDS
910 920 930 940 950
GEPELCAYVE GLQRNEVRAQ LQRLLPGYMV PAYMIEMEQW PVTPSGKLDR
960 970 980 990 1000
NALPAPGGAA DAETYTAPRN VTEMKLSQLW EDVLKNGPVG IHDNFFDRGG
1010 1020 1030 1040 1050
HSLKATALVS RIAKEFDVQV PLKDVFAHPT VEGLATVIRE GTDSPYEAIK
1060 1070 1080 1090 1100
PAEKQETYPV SSAQKRIYVL QQLEDGGTGY NMPAVLELEG KLNLERMDRA
1110 1120 1130 1140 1150
FKELIKRHES LRTAFEQDAG GDPVQRIHDE VPFTLQTTVL GARTEEEAAA
1160 1170 1180 1190 1200
AFIKPFDLSQ APLFRAQIVK VSDERHLLLV DMHHIISDGV SVNILIREFG
1210 1220 1230 1240 1250
ELYNNRKLPA LRIQYKDYAV WQEGFKTGDA YKTQGAYWLK QLEGELPVLD
1260 1270 1280 1290 1300
LPADHARPPM RSFAGDKVSF TLDQEVTSGL YKLARENGST LYMVLLAAYT
1310 1320 1330 1340 1350
AFLSRLSGQE DIIVGSPIAG RPHKDLEPIL GMFVNTLALR TRPEGGKPFV
1360 1370 1380 1390 1400
QYLQEVRETA MEAFEHQDYP FEELVDKLEL TRDMSRNPLF DVMFVLQNMD
1410 1420 1430 1440 1450
QESLELDELC LKPAANNGHQ TSKFDLTLYA QEQPRGLLTF QMEFSTDLYK
1460 1470 1480 1490 1500
KKTIEKWLQY FNNMLLSIIK DNKAALGTIN ILNEDEAHYL IHELNRTKID
1510 1520 1530 1540 1550
YPRNETISRL FEMQAEQTPN AVAIVSDTQV FTYEDLNSWA NQIASVLQIK
1560 1570 1580 1590 1600
GVGPDSVVAL LTGRTPELIA GMLGILKAGG AYLPIDSNLP VERIAYMLSD
1610 1620 1630 1640 1650
SRAALLLQSE KTEKRLLGIE CEQIIIEDIQ KQGEAKNVES SAGPHSLAYI
1660 1670 1680 1690 1700
IYTSGSTGKP KGVMIEQRSV IRLVKNSNYI TFTPEDRLLM TSSIGFDVGS
1710 1720 1730 1740 1750
FEIFGPLLNG AALHLSDQQT FLDSHQLKRY IEHQGITTIW LTSSLFNHLT
1760 1770 1780 1790 1800
EQNEQTFSQL KHLIIGGEAL SPSHVNRIRN VCPEVSIWNG YGPTENTTFS
1810 1820 1830 1840 1850
TCLHIQKTYE LSIPIGRPVG NSTAFILNQW GVLQPVGAVG ELCVGGDGVA
1860 1870 1880 1890 1900
RGYLGRPDLT KEKFVPHPFA PGDRLYRTGD LARWLSDGTI EYVGRIDDQV
1910 1920 1930 1940 1950
KVRGYRVELG EIETALRQID GVKEAAVLAR TAQTGSKELF GYISVKAGTN
1960 1970 1980 1990 2000
AEQVRSLLAR SLPNYMIPAY IIEMETLPLT SNGKLNRKAL PEPDVASKQT
2010 2020 2030 2040 2050
YIPPRNELEE QLALIWQEVL GIQRIGIEDS FFELGGDSIK ALQVSARLGR
2060 2070 2080 2090 2100
YGLSLQVSDL FRHPKIKDLS PFIRKSERII EQGPIQGDVP WTPVQQWFFS
2110 2120 2130 2140 2150
QDIEERHHFN QSVMLFHSGR LSENALRPAL KKLAEHHDAL RMVYRNDDRR
2160 2170 2180 2190 2200
WIQINQGIHE SQLYSLRISD LSQSESGWET KIKQEVADLQ QSINLQEGPL
2210 2220 2230 2240 2250
LHAALFKTLT GDYLFLAIHH LVVDGVSWRI LLEDLSAGYQ QAAAGQTIQL
2260 2270 2280 2290 2300
PPKTDSYQEY ARRIQEYAQS SKLIREEAYW RSVEEQQAAE LPYEIPHHVN
2310 2320 2330 2340 2350
IDFSKRDSLS FSLTEADTAV LLQNVNHAYG TDTQDILLTA ASLAICEWTG
2360 2370 2380 2390 2400
GSKLRIAMEG HGREHILPEL DISRTVGWFT SMYPALISFE NHRDELGTSV
2410 2420 2430 2440 2450
KTVKDTLGRI PNKGVGYGML KYLTHPENKS ITFSKTPEIS FNYLGQFNDI
2460 2470 2480 2490 2500
ERQDTFRPSS LGSGKDITHT WKREQIIEMS AMAADKKLHF NLSYPPARFH
2510 2520 2530 2540 2550
RNTMEQLINR IEHFLLDIMK HCAGQQKAEK TLSDFSSQSL TAEDLDSISS

LVEEL
Length:2,555
Mass (Da):287,503
Last modified:July 15, 1999 - v2
Checksum:i3E50B3395105D5D0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAB13715.1.
Z34883 Genomic DNA. Translation: CAA84362.1.
PIRiC69681.
RefSeqiNP_389714.1. NC_000964.3.
WP_009967356.1. NZ_CP010052.1.

Genome annotation databases

EnsemblBacteriaiCAB13715; CAB13715; BSU18320.
GeneIDi940102.
KEGGibsu:BSU18320.
PATRICi18975501. VBIBacSub10457_1942.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAB13715.1.
Z34883 Genomic DNA. Translation: CAA84362.1.
PIRiC69681.
RefSeqiNP_389714.1. NC_000964.3.
WP_009967356.1. NZ_CP010052.1.

3D structure databases

ProteinModelPortaliP39847.
SMRiP39847. Positions 443-958, 963-1482, 1485-1995, 1998-2076.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP39847. 16 interactions.
STRINGi224308.Bsubs1_010100010096.

Proteomic databases

PaxDbiP39847.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13715; CAB13715; BSU18320.
GeneIDi940102.
KEGGibsu:BSU18320.
PATRICi18975501. VBIBacSub10457_1942.

Phylogenomic databases

eggNOGiENOG4105C0W. Bacteria.
COG1020. LUCA.
HOGENOMiHOG000229991.
InParanoidiP39847.
KOiK15666.
OMAiMVQHHIV.
OrthoDBiEOG6XHC08.

Enzyme and pathway databases

BioCyciBSUB:BSU18320-MONOMER.

Family and domain databases

Gene3Di1.10.1200.10. 2 hits.
InterProiIPR010071. AA_adenyl_domain.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR001242. Condensatn.
IPR010060. NRPS_synth.
IPR020806. PKS_PP-bd.
IPR009081. PP-bd_ACP.
IPR006162. Ppantetheine_attach_site.
[Graphical view]
PfamiPF00501. AMP-binding. 2 hits.
PF13193. AMP-binding_C. 2 hits.
PF00668. Condensation. 3 hits.
PF00550. PP-binding. 2 hits.
[Graphical view]
SMARTiSM00823. PKS_PP. 2 hits.
[Graphical view]
SUPFAMiSSF47336. SSF47336. 2 hits.
TIGRFAMsiTIGR01733. AA-adenyl-dom. 2 hits.
TIGR01720. NRPS-para261. 1 hit.
PROSITEiPS50075. ACP_DOMAIN. 2 hits.
PS00455. AMP_BINDING. 2 hits.
PS00012. PHOSPHOPANTETHEINE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  2. "A putative new peptide synthase operon in Bacillus subtilis: partial characterization."
    Tognoni A., Franchi E., Magistrelli C., Colombo E., Cosmina P., Grandi G.
    Microbiology 141:645-648(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-859.
    Strain: 168.
  3. "The genes degQ, pps, and lpa-8 (sfp) are responsible for conversion of Bacillus subtilis 168 to plipastatin production."
    Tsuge K., Ano T., Hirai M., Nakamura Y., Shoda M.
    Antimicrob. Agents Chemother. 43:2183-2192(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN PLIPASTATIN BIOSYNTHESIS.

Entry informationi

Entry nameiPPSC_BACSU
AccessioniPrimary (citable) accession number: P39847
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 15, 1999
Last modified: May 11, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.