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Protein

Plipastatin synthase subunit C

Gene

ppsC

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This protein is a multifunctional enzyme, able to activate and polymerize the amino acids Glu and Ala/Val as part of the biosynthesis of the lipopeptide antibiotic plipastatin. The Ala/Val residue is further epimerized to the D-isomer form. The activation sites for these amino acids consist of individual domains.1 Publication

Cofactori

pantetheine 4'-phosphateCuratedNote: Binds 2 phosphopantetheines covalently.Curated

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase, Multifunctional enzyme, Transferase
Biological processAntibiotic biosynthesis

Enzyme and pathway databases

BioCyciBSUB:BSU18320-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Plipastatin synthase subunit C (EC:2.3.1.-)
Alternative name(s):
Peptide synthase 3
Including the following 2 domains:
ATP-dependent glutamate adenylase 2
Short name:
GluA 2
Alternative name(s):
Glutamate activase 2
ATP-dependent alanine/valine adenylase
Short name:
Ala/ValA
Alternative name(s):
Alanine/valine activase
Gene namesi
Name:ppsC
Synonyms:pps3
Ordered Locus Names:BSU18320
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001931871 – 2555Plipastatin synthase subunit CAdd BLAST2555

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1002O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation1
Modified residuei2038O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation1

Keywords - PTMi

Phosphopantetheine, Phosphoprotein

Proteomic databases

PaxDbiP39847

Interactioni

Protein-protein interaction databases

IntActiP39847, 16 interactors
STRINGi224308.Bsubs1_010100010096

Structurei

3D structure databases

ProteinModelPortaliP39847
SMRiP39847
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini967 – 1042Carrier 1PROSITE-ProRule annotationAdd BLAST76
Domaini2003 – 2077Carrier 2PROSITE-ProRule annotationAdd BLAST75

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni7 – 306Condensation 1Add BLAST300
Regioni491 – 894Adenylation 1Add BLAST404
Regioni1054 – 1344Condensation 2Add BLAST291
Regioni1532 – 1927Adenylation 2Add BLAST396
Regioni2085 – 2548Epimerization 3Add BLAST464

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1148 – 1151Poly-Ala4

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105C0W Bacteria
COG1020 LUCA
HOGENOMiHOG000229991
InParanoidiP39847
KOiK15666
OMAiLFDFWSH

Family and domain databases

Gene3Di1.10.1200.10, 2 hits
3.30.559.10, 5 hits
InterProiView protein in InterPro
IPR010071 AA_adenyl_domain
IPR036736 ACP-like_sf
IPR025110 AMP-bd_C
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
IPR023213 CAT-like_dom_sf
IPR001242 Condensatn
IPR010060 NRPS_synth
IPR020806 PKS_PP-bd
IPR009081 PP-bd_ACP
IPR006162 Ppantetheine_attach_site
PfamiView protein in Pfam
PF00501 AMP-binding, 2 hits
PF13193 AMP-binding_C, 2 hits
PF00668 Condensation, 3 hits
PF00550 PP-binding, 2 hits
SMARTiView protein in SMART
SM00823 PKS_PP, 2 hits
SUPFAMiSSF47336 SSF47336, 2 hits
TIGRFAMsiTIGR01733 AA-adenyl-dom, 2 hits
TIGR01720 NRPS-para261, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 2 hits
PS50075 CARRIER, 2 hits
PS00012 PHOSPHOPANTETHEINE, 1 hit

Sequencei

Sequence statusi: Complete.

P39847-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPQQPEIQDI YPLSFMQEGM LFHSLYDEQS RAYFEQASFT IHGQLDLERF
60 70 80 90 100
QKSMDAVFDR YDIFRTAFIY KNVAKPRQVV LKQRHCPIHI EDISHLNERD
110 120 130 140 150
KEHCTEAFKE QDKSKGFDLQ TDVLMRISIL KWAPDHYVCI WSHHHILMDG
160 170 180 190 200
WCLGIVIKDF LHIYQALGKG QLPDLPPVQP YGTYIKWLMQ QDREEAAEYW
210 220 230 240 250
KKRLQHFEKS TPLPKRTDQI PNGTLQQITF AIPEKETAEL QKIAAASGAT
260 270 280 290 300
LNTVFQALWG IMLQKVNRSS DAVFGSVISG RPSELKDVEN MVGLFINTIP
310 320 330 340 350
IRAQSDSLSF SDLVRRMQKD MNEAEAYSYF PLYDIQAQSA LKQELIDHII
360 370 380 390 400
VFENTPTQQE IEELNQAGSF DFSVKDFEME EVTNYSCSVK VIPGRTLYVR
410 420 430 440 450
IHFQTSAYQP SMMSEIKDYL LHMVSDVISD PSLPVSKMTL LDEDKTRKIV
460 470 480 490 500
SQNNRTVSVS PEAPTLHGLF ERQAAVTPER LAIRFSGGSL TYAELDMYAS
510 520 530 540 550
RLAAHLAARG VTNESIVGVL SERSPDMLIA VLAVLKAGGA YLPLDPAYPK
560 570 580 590 600
ERLSYMLKDS GASLLLTQPG CSAPNFSGET LEVDMTSLEC EEVKRHVSAS
610 620 630 640 650
VSDGSLAYVI YTSGSTGQPK GVAVEHRQAV SFLTGMQHQF RLSEDDIVMV
660 670 680 690 700
KTSFSFDASV WQLFWWALSG ASAYLLPPGW EKDSALIVQA IHQENVTTAH
710 720 730 740 750
FIPAMLNSFL DQAEIERLSD RTSLKRVFAG GEPLAPRTAA RFASVLPQVS
760 770 780 790 800
LIHGYGPTEA TVDAAFYVLD PERDRDRLRI PIGKPVPGAR LYVLDPHLAV
810 820 830 840 850
QPSGVAGELY IAGAGVARGY LNRPALTEER FLEDPFYLGE RMYKTGDVAR
860 870 880 890 900
WLPDGNVEFL GRTDDQVKIR GYRIEPGEIE AALRSIEGVR EAAVTVRTDS
910 920 930 940 950
GEPELCAYVE GLQRNEVRAQ LQRLLPGYMV PAYMIEMEQW PVTPSGKLDR
960 970 980 990 1000
NALPAPGGAA DAETYTAPRN VTEMKLSQLW EDVLKNGPVG IHDNFFDRGG
1010 1020 1030 1040 1050
HSLKATALVS RIAKEFDVQV PLKDVFAHPT VEGLATVIRE GTDSPYEAIK
1060 1070 1080 1090 1100
PAEKQETYPV SSAQKRIYVL QQLEDGGTGY NMPAVLELEG KLNLERMDRA
1110 1120 1130 1140 1150
FKELIKRHES LRTAFEQDAG GDPVQRIHDE VPFTLQTTVL GARTEEEAAA
1160 1170 1180 1190 1200
AFIKPFDLSQ APLFRAQIVK VSDERHLLLV DMHHIISDGV SVNILIREFG
1210 1220 1230 1240 1250
ELYNNRKLPA LRIQYKDYAV WQEGFKTGDA YKTQGAYWLK QLEGELPVLD
1260 1270 1280 1290 1300
LPADHARPPM RSFAGDKVSF TLDQEVTSGL YKLARENGST LYMVLLAAYT
1310 1320 1330 1340 1350
AFLSRLSGQE DIIVGSPIAG RPHKDLEPIL GMFVNTLALR TRPEGGKPFV
1360 1370 1380 1390 1400
QYLQEVRETA MEAFEHQDYP FEELVDKLEL TRDMSRNPLF DVMFVLQNMD
1410 1420 1430 1440 1450
QESLELDELC LKPAANNGHQ TSKFDLTLYA QEQPRGLLTF QMEFSTDLYK
1460 1470 1480 1490 1500
KKTIEKWLQY FNNMLLSIIK DNKAALGTIN ILNEDEAHYL IHELNRTKID
1510 1520 1530 1540 1550
YPRNETISRL FEMQAEQTPN AVAIVSDTQV FTYEDLNSWA NQIASVLQIK
1560 1570 1580 1590 1600
GVGPDSVVAL LTGRTPELIA GMLGILKAGG AYLPIDSNLP VERIAYMLSD
1610 1620 1630 1640 1650
SRAALLLQSE KTEKRLLGIE CEQIIIEDIQ KQGEAKNVES SAGPHSLAYI
1660 1670 1680 1690 1700
IYTSGSTGKP KGVMIEQRSV IRLVKNSNYI TFTPEDRLLM TSSIGFDVGS
1710 1720 1730 1740 1750
FEIFGPLLNG AALHLSDQQT FLDSHQLKRY IEHQGITTIW LTSSLFNHLT
1760 1770 1780 1790 1800
EQNEQTFSQL KHLIIGGEAL SPSHVNRIRN VCPEVSIWNG YGPTENTTFS
1810 1820 1830 1840 1850
TCLHIQKTYE LSIPIGRPVG NSTAFILNQW GVLQPVGAVG ELCVGGDGVA
1860 1870 1880 1890 1900
RGYLGRPDLT KEKFVPHPFA PGDRLYRTGD LARWLSDGTI EYVGRIDDQV
1910 1920 1930 1940 1950
KVRGYRVELG EIETALRQID GVKEAAVLAR TAQTGSKELF GYISVKAGTN
1960 1970 1980 1990 2000
AEQVRSLLAR SLPNYMIPAY IIEMETLPLT SNGKLNRKAL PEPDVASKQT
2010 2020 2030 2040 2050
YIPPRNELEE QLALIWQEVL GIQRIGIEDS FFELGGDSIK ALQVSARLGR
2060 2070 2080 2090 2100
YGLSLQVSDL FRHPKIKDLS PFIRKSERII EQGPIQGDVP WTPVQQWFFS
2110 2120 2130 2140 2150
QDIEERHHFN QSVMLFHSGR LSENALRPAL KKLAEHHDAL RMVYRNDDRR
2160 2170 2180 2190 2200
WIQINQGIHE SQLYSLRISD LSQSESGWET KIKQEVADLQ QSINLQEGPL
2210 2220 2230 2240 2250
LHAALFKTLT GDYLFLAIHH LVVDGVSWRI LLEDLSAGYQ QAAAGQTIQL
2260 2270 2280 2290 2300
PPKTDSYQEY ARRIQEYAQS SKLIREEAYW RSVEEQQAAE LPYEIPHHVN
2310 2320 2330 2340 2350
IDFSKRDSLS FSLTEADTAV LLQNVNHAYG TDTQDILLTA ASLAICEWTG
2360 2370 2380 2390 2400
GSKLRIAMEG HGREHILPEL DISRTVGWFT SMYPALISFE NHRDELGTSV
2410 2420 2430 2440 2450
KTVKDTLGRI PNKGVGYGML KYLTHPENKS ITFSKTPEIS FNYLGQFNDI
2460 2470 2480 2490 2500
ERQDTFRPSS LGSGKDITHT WKREQIIEMS AMAADKKLHF NLSYPPARFH
2510 2520 2530 2540 2550
RNTMEQLINR IEHFLLDIMK HCAGQQKAEK TLSDFSSQSL TAEDLDSISS

LVEEL
Length:2,555
Mass (Da):287,503
Last modified:July 15, 1999 - v2
Checksum:i3E50B3395105D5D0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA Translation: CAB13715.1
Z34883 Genomic DNA Translation: CAA84362.1
PIRiC69681
RefSeqiNP_389714.1, NC_000964.3
WP_009967356.1, NZ_CP019663.1

Genome annotation databases

EnsemblBacteriaiCAB13715; CAB13715; BSU18320
GeneIDi940102
KEGGibsu:BSU18320
PATRICifig|224308.179.peg.1999

Similar proteinsi

Entry informationi

Entry nameiPPSC_BACSU
AccessioniPrimary (citable) accession number: P39847
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 15, 1999
Last modified: March 28, 2018
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health