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Protein

Aldose 1-epimerase

Gene

galM

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Alpha-D-glucose = beta-D-glucose.1 Publication

Pathwayi: hexose metabolism

This protein is involved in the pathway hexose metabolism, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway hexose metabolism and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei177 – 1771Proton donorBy similarity
Binding sitei230 – 2301SubstrateBy similarity
Active sitei283 – 2831Proton acceptorBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

BioCyciBSUB:BSU18360-MONOMER.
UniPathwayiUPA00242.

Names & Taxonomyi

Protein namesi
Recommended name:
Aldose 1-epimerase (EC:5.1.3.3)
Alternative name(s):
Galactose mutarotase
Gene namesi
Name:galM
Synonyms:yoxA
Ordered Locus Names:BSU18360
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 325324Aldose 1-epimerasePRO_0000049666Add
BLAST

Proteomic databases

PaxDbiP39840.

Expressioni

Developmental stagei

Expressed at the end of the exponential growth phase.1 Publication

Inductioni

By nitrogen or tryptophan starvation.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100010116.

Structurei

Secondary structure

1
325
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 108Combined sources
Beta strandi13 – 2816Combined sources
Turni29 – 335Combined sources
Beta strandi34 – 407Combined sources
Turni41 – 444Combined sources
Helixi54 – 596Combined sources
Beta strandi63 – 653Combined sources
Beta strandi68 – 714Combined sources
Beta strandi73 – 764Combined sources
Beta strandi79 – 824Combined sources
Beta strandi85 – 884Combined sources
Turni94 – 974Combined sources
Beta strandi98 – 1036Combined sources
Beta strandi110 – 11910Combined sources
Beta strandi121 – 1288Combined sources
Helixi129 – 1313Combined sources
Helixi133 – 1386Combined sources
Beta strandi143 – 15210Combined sources
Beta strandi155 – 16410Combined sources
Beta strandi166 – 1683Combined sources
Beta strandi170 – 1723Combined sources
Turni184 – 1863Combined sources
Beta strandi187 – 19913Combined sources
Beta strandi205 – 2117Combined sources
Helixi215 – 2206Combined sources
Beta strandi230 – 2367Combined sources
Helixi237 – 2404Combined sources
Beta strandi245 – 2506Combined sources
Turni251 – 2544Combined sources
Beta strandi255 – 2617Combined sources
Beta strandi267 – 2715Combined sources
Beta strandi277 – 28711Combined sources
Helixi291 – 2933Combined sources
Helixi298 – 3014Combined sources
Beta strandi304 – 3063Combined sources
Beta strandi311 – 32212Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3MWXX-ray1.45A/B1-325[»]
4BZEX-ray2.00A/B1-325[»]
4BZFX-ray1.90A/B2-325[»]
4BZGX-ray2.13A/B2-325[»]
4BZHX-ray2.60A/B2-325[»]
ProteinModelPortaliP39840.
SMRiP39840. Positions 2-323.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP39840.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni73 – 742Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the aldose epimerase family.Curated

Phylogenomic databases

eggNOGiENOG4108N2V. Bacteria.
COG2017. LUCA.
HOGENOMiHOG000008952.
InParanoidiP39840.
KOiK01785.
OMAiWGSNVIS.
OrthoDBiEOG6358CK.
PhylomeDBiP39840.

Family and domain databases

Gene3Di2.70.98.10. 1 hit.
InterProiIPR008183. Aldose_1/G6P_1-epimerase.
IPR011013. Gal_mutarotase_SF_dom.
IPR014718. GH-type_carb-bd.
[Graphical view]
PfamiPF01263. Aldose_epim. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P39840-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANFIEKITY LGTPAIKAGN EHLEMIVVPE WGSNVISLVD KTTNVQLLRE
60 70 80 90 100
PETAESFHDT PTLYGIPILF PPNRISDGTF SFRGRTYHFD INEKDKHNHL
110 120 130 140 150
HGFLYHEKWN VVTTKQTDEG VIVETEIDLS ELPHVQKQFP HHAVVRMTYT
160 170 180 190 200
IKENTLFKHA TVMNKGKEAF PWGIGYHTTF IFPAESSLFS LTADQQWELD
210 220 230 240 250
ERLLPTGKLM DVPYKEALHE GMDLRHKQLD DVFLSSYQKR GGENQAVIYH
260 270 280 290 300
QHAHISIIYK ADEQFKHWVV YNADGKQGYL CPEPYTWVTN AVNLDLPSSL
310 320
TGLQVLEPGE ETTAKSSITI ELNHQ
Length:325
Mass (Da):37,228
Last modified:December 15, 1998 - v2
Checksum:iCD80CB777AF4B829
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAB13719.1.
Z34883 Genomic DNA. Translation: CAA84365.1.
PIRiA69930.
RefSeqiNP_389718.1. NC_000964.3.
WP_004399304.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB13719; CAB13719; BSU18360.
GeneIDi938046.
KEGGibsu:BSU18360.
PATRICi18975509. VBIBacSub10457_1946.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAB13719.1.
Z34883 Genomic DNA. Translation: CAA84365.1.
PIRiA69930.
RefSeqiNP_389718.1. NC_000964.3.
WP_004399304.1. NZ_JNCM01000036.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3MWXX-ray1.45A/B1-325[»]
4BZEX-ray2.00A/B1-325[»]
4BZFX-ray1.90A/B2-325[»]
4BZGX-ray2.13A/B2-325[»]
4BZHX-ray2.60A/B2-325[»]
ProteinModelPortaliP39840.
SMRiP39840. Positions 2-323.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100010116.

Proteomic databases

PaxDbiP39840.

Protocols and materials databases

DNASUi938046.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13719; CAB13719; BSU18360.
GeneIDi938046.
KEGGibsu:BSU18360.
PATRICi18975509. VBIBacSub10457_1946.

Phylogenomic databases

eggNOGiENOG4108N2V. Bacteria.
COG2017. LUCA.
HOGENOMiHOG000008952.
InParanoidiP39840.
KOiK01785.
OMAiWGSNVIS.
OrthoDBiEOG6358CK.
PhylomeDBiP39840.

Enzyme and pathway databases

UniPathwayiUPA00242.
BioCyciBSUB:BSU18360-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP39840.

Family and domain databases

Gene3Di2.70.98.10. 1 hit.
InterProiIPR008183. Aldose_1/G6P_1-epimerase.
IPR011013. Gal_mutarotase_SF_dom.
IPR014718. GH-type_carb-bd.
[Graphical view]
PfamiPF01263. Aldose_epim. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the proteins encoded by the Bacillus subtilis yoxA-dacC operon."
    Duez C., Zervosen A., Teller N., Melkonian R., Banzubaze E., Bouillenne F., Luxen A., Frere J.-M.
    FEMS Microbiol. Lett. 300:42-47(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-5, CATALYTIC ACTIVITY, INDUCTION, DEVELOPMENTAL STAGE.
    Strain: 168.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  3. "A putative new peptide synthase operon in Bacillus subtilis: partial characterization."
    Tognoni A., Franchi E., Magistrelli C., Colombo E., Cosmina P., Grandi G.
    Microbiology 141:645-648(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 284-325.
    Strain: 168.

Entry informationi

Entry nameiGALM_BACSU
AccessioniPrimary (citable) accession number: P39840
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: December 15, 1998
Last modified: July 6, 2016
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.