High-affinity gluconate transporter - Escherichia coli (strain K12)

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P39835 (GNTT_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 102. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
High-affinity gluconate transporter

Alternative name(s):
Gluconate permease
Gnt-I system

Gene names

Name:	gntT
Synonyms:	gntM, usgA, yhgC
Ordered Locus Names:	b3415, JW5690

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	438 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of the gluconate utilization system Gnt-I; high-affinity intake of gluconate.
Pathway	Carbohydrate acid metabolism; D-gluconate degradation.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Ref.9.
Sequence similarities	Belongs to the GntP permease family.

Ontologies

Keywords
Biological process	Gluconate utilization Sugar transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	D-gluconate catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane Inferred from direct assay Ref.8. Source: EcoCyc plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	gluconate transmembrane transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 438

438

High-affinity gluconate transporter

PRO_0000061934

Regions

Transmembrane

2 – 22

Helical; Potential

Transmembrane

23 – 43

Helical; Potential

Transmembrane

52 – 72

Helical; Potential

Transmembrane

108 – 128

Helical; Potential

Transmembrane

134 – 154

Helical; Potential

Transmembrane

174 – 194

Helical; Potential

Transmembrane

222 – 242

Helical; Potential

Transmembrane

258 – 278

Helical; Potential

Transmembrane

292 – 312

Helical; Potential

Transmembrane

327 – 347

Helical; Potential

Transmembrane

349 – 369

Helical; Potential

Transmembrane

370 – 390

Helical; Potential

Transmembrane

418 – 438

Helical; Potential

Experimental info

Sequence conflict

55 – 58

GGTL → ADV in AAA58213. Ref.1

Sequence conflict

385

S → A Ref.5

Sequence conflict

420 – 421

ET → VS Ref.5

Sequences

Sequence

Length

Mass (Da)

Tools

P39835 [UniParc].

Last modified October 11, 2004. Version 3.
Checksum: C142882C557E894C
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FASTA

438

45,967

        10         20         30         40         50         60 
MPLVIVAIGV ILLLLLMIRF KMNGFIALVL VALAVGLMQG MPLDKVIGSI KAGVGGTLGS 

        70         80         90        100        110        120 
LALIMGFGAM LGKMLADCGG AQRIATTLIA KFGKKHIQWA VVLTGFTVGF ALFYEVGFVL 

       130        140        150        160        170        180 
MLPLVFTIAA SANIPLLYVG VPMAAALSVT HGFLPPHPGP TAIATIFNAD MGKTLLYGTI 

       190        200        210        220        230        240 
LAIPTVILAG PVYARVLKGI DKPIPEGLYS AKTFSEEEMP SFGVSVWTSL VPVVLMAMRA 

       250        260        270        280        290        300 
IAEMILPKGH AFLPVAEFLG DPVMATLIAV LIAMFTFGLN RGRSMDQIND TLVSSIKIIA 

       310        320        330        340        350        360 
MMLLIIGGGG AFKQVLVDSG VDKYIASMMH ETNISPLLMA WSIAAVLRIA LGSATVAAIT 

       370        380        390        400        410        420 
AGGIAAPLIA TTGVSPELMV IAVGSGSVIF SHVNDPGFWL FKEYFNLTIG ETIKSWSMLE 

       430 
TIISVCGLVG CLLLNMVI

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the Escherichia coli gntT and gntU genes and comparison of the products with their homologues." Yamada M., Kawai T., Izu H. Biosci. Biotechnol. Biochem. 60:1548-1550(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Escherichia coli K-12: a cooperatively developed annotation snapshot -- 2005." Riley M., Abe T., Arnaud M.B., Berlyn M.K.B., Blattner F.R., Chaudhuri R.R., Glasner J.D., Horiuchi T., Keseler I.M., Kosuge T., Mori H., Perna N.T., Plunkett G. III, Rudd K.E., Serres M.H., Thomas G.H., Thomson N.R., Wishart D., Wanner B.L. Nucleic Acids Res. 34:1-9(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION TO 55-58.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Molecular characterization of malQ, the structural gene for the Escherichia coli enzyme amylomaltase." Pugsley A.P., Dubreuil C. Mol. Microbiol. 2:473-479(1988) [PubMed] [Europe PMC] [Abstract] Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 381-437.
[6]	"Intrinsic and extrinsic approaches for detecting genes in a bacterial genome." Borodovsky M., Rudd K.E., Koonin E.V. Nucleic Acids Res. 22:4756-4767(1994) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[7]	"Mutations affecting gluconate catabolism in Escherichia coli. Genetic mapping of the locus for the thermosensitive gluconokinase." Isturiz T., Palmero E., Vitelli-Flores J. J. Gen. Microbiol. 132:3209-3219(1986) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[8]	"Molecular genetic characterization of the Escherichia coli gntT gene of GntI, the main system for gluconate metabolism." Porco A., Peekhaus N., Bausch C., Tong S., Isturiz T., Conway T. J. Bacteriol. 179:1584-1590(1997) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[9]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18997 Genomic DNA. Translation: AAA58213.1. U00096 Genomic DNA. Translation: AAT48179.1. AP009048 Genomic DNA. Translation: BAE77876.1. M32793 Genomic DNA. No translation available.
PIR	JC4988.
RefSeq	YP_026217.1. NC_000913.2. YP_492017.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P39835.
ModBase	Search...
Protein-protein interaction databases
STRING	511145.b3415.
Protein family/group databases
TCDB	2.A.8.1.4. gluconate:H+ symporter (GntP) family.
Protocols and materials databases
DNASU	947924.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAT48179; AAT48179; b3415. BAE77876; BAE77876; BAE77876.
GeneID	12932223. 947924.
KEGG	ecj:Y75_p3761. eco:b3415.
PATRIC	32122268. VBIEscCol129921_3511.
Organism-specific databases
EchoBASE	EB2282.
EcoGene	EG12380. gntT.
Phylogenomic databases
eggNOG	COG2610.
HOGENOM	HOG000237115.
KO	K06155.
OMA	WAIMVAS.
ProtClustDB	PRK14984.
Enzyme and pathway databases
BioCyc	EcoCyc:GNTT-MONOMER. ECOL316407:JW5690-MONOMER.
UniPathway	UPA00792.
Gene expression databases
Genevestigator	P39835.
Family and domain databases
InterPro	IPR003474. Glcn_transporter. [Graphical view]
Pfam	PF02447. GntP_permease. 1 hit. [Graphical view]
PIRSF	PIRSF002746. Gluconate_transporter. 1 hit.
TIGRFAMs	TIGR00791. gntP. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GNTT_ECOLI

Accession

Primary (citable) accession number: P39835
Secondary accession number(s): Q2M780, Q6BF35

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 102 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents