P39825 (PROF_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Profilin

Gene names

Name:	cdc3
ORF Names:	SPAC4A8.15c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	127 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. In S.pombe, it is essential for cytokinesis.
Subunit structure	Occurs in many kinds of cells as a complex with monomeric actin in a 1:1 ratio.
Subcellular location	Cytoplasm › cytoskeleton.
Sequence similarities	Belongs to the profilin family.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Ligand	Actin-binding
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin filament bundle assembly involved in cytokinetic actomyosin contractile ring assembly Non-traceable author statement. Source: PomBase actin filament polymerization Inferred from mutant phenotype PubMed 11231572. Source: PomBase plasma membrane fusion involved in cytogamy Inferred from mutant phenotype PubMed 9490631. Source: PomBase
Cellular_component	actin cortical patch Traceable author statement PubMed 8978670. Source: PomBase cell cortex of cell tip Inferred from direct assay Ref.1. Source: PomBase cytosol Inferred from direct assay PubMed 16823372. Source: PomBase mating projection Inferred from direct assay PubMed 9490631. Source: PomBase medial cortex Inferred from direct assay Ref.1. Source: PomBase nucleus Inferred from direct assay PubMed 16823372. Source: PomBase
Molecular_function	actin monomer binding Inferred from direct assay PubMed 11294914. Source: PomBase guanyl-nucleotide exchange factor activity Inferred from direct assay PubMed 11294914. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 127

127

Profilin

PRO_0000199607

Secondary structure

1

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127

Helix Strand Turn

Details...

Helix

3 – 8

6

Turn

9 – 11

3

Helix

12 – 14

3

Beta strand

19 – 24

6

Beta strand

30 – 33

4

Helix

41 – 52

12

Helix

56 – 60

5

Beta strand

62 – 64

3

Beta strand

67 – 74

8

Beta strand

76 – 83

8

Beta strand

86 – 92

7

Beta strand

94 – 102

9

Helix

108 – 125

18

Sequences

	Sequence		Length	Mass (Da)
	P39825 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 1D3BE221AAA73594	FASTA	127	13,411
	10 20 30 40 50 60 MSWQAYVDTS LLGTGKIDRA AIVSRAGDSV WAASAGFNLS PQEIQGLAAG FQDPPSMFGT 70 80 90 100 110 120 GIILAGQKYI TIRAEGRSIY GKLQKEGIIC VATKLCILVS HYPETTLPGE AAKITEALAD YLVGVGY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Schizosaccharomyces pombe cdc3+ gene encodes a profilin essential for cytokinesis." Balasubramanian M.K., Hirani B.R., Burke J.D., Gould K.L. J. Cell Biol. 125:1289-1301(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z30648 Genomic DNA. No translation available.
CU329670 Genomic DNA. Translation: CAB38578.1.

PIR

A53952.

RefSeq

NP_593827.1. NM_001019256.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3D9Y	X-ray	1.65	A/B	1-127	[»]
3DAV	X-ray	2.20	A/B	1-127	[»]

ProteinModelPortal

P39825.

SMR

P39825. Positions 2-127.

ModBase

Search...

Protein-protein interaction databases

STRING

4896.SPAC4A8.15c-1.

Proteomic databases

PaxDb

P39825.

PRIDE

P39825.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

SPAC4A8.15c.1; SPAC4A8.15c.1:pep; SPAC4A8.15c.

GeneID

2543526.

KEGG

spo:SPAC4A8.15c.

Organism-specific databases

PomBase

SPAC4A8.15c.

Phylogenomic databases

eggNOG

NOG277929.

HOGENOM

HOG000171591.

KO

K05759.

OMA

QLLASQC.

OrthoDB

EOG4X9BSP.

Family and domain databases

InterPro

IPR027310. Profilin_CS.
IPR005455. Profilin_eukaryotes/bac.
[Graphical view]

PANTHER

PTHR11604. PTHR11604. 1 hit.

Pfam

PF00235. Profilin. 1 hit.
[Graphical view]

PRINTS

PR00392. PROFILIN.
PR01640. PROFILINPLNT.

SMART

SM00392. PROF. 1 hit.
[Graphical view]

SUPFAM

SSF55770. Profilin. 1 hit.

PROSITE

PS00414. PROFILIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P39825.

NextBio

20804536.

Entry information

Entry name

PROF_SCHPO

Accession

Primary (citable) accession number: P39825

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program