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Protein

Beta-lactamase

Gene

penP

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This protein is a beta-lactamase with a substrate specificity for penicillins.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei89Acyl-ester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Enzyme and pathway databases

BioCyciBSUB:BSU18800-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase (EC:3.5.2.6)
Alternative name(s):
Penicillinase
Gene namesi
Name:penP
Ordered Locus Names:BSU18800
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Secreted 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 361 PublicationAdd BLAST36
ChainiPRO_000001697537 – 306Beta-lactamaseAdd BLAST270

Proteomic databases

PaxDbiP39824.
PRIDEiP39824.

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100010361.

Structurei

3D structure databases

ProteinModelPortaliP39824.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni251 – 253Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the class-A beta-lactamase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108J4B. Bacteria.
COG2367. LUCA.
HOGENOMiHOG000201073.
InParanoidiP39824.
KOiK17836.
OMAiDEKEATY.
PhylomeDBiP39824.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P39824-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLKTKASIK FGICVGLLCL SITGFTPFFN STHAEAKSIE DTNMASCITN
60 70 80 90 100
KKFVQLEKKF DARLGVYAID IGSNKTIAYR PNERFAYAST YKVLAAAAVL
110 120 130 140 150
KKNSIEKLNE VIHYSKDDLV TYSPITEKHL DTGMSLKEIS EAAIRYSDNT
160 170 180 190 200
AGNILLQQLG GPKGFEKSLK QIGDHVTKAK RFETDLNSAI PGDIRDTSTA
210 220 230 240 250
KALATDLKAF TLDNTLTTDK RMILTDWMRG NATGDELIRA GAPIGWEVGD
260 270 280 290 300
KSGAGSYGTR NDIAIVWPPN RAPIVVAILS NRFTKDANYD NALIAEAAKV

VLNDLK
Length:306
Mass (Da):33,446
Last modified:May 30, 2000 - v2
Checksum:i95BE5644B731500E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti51K → N in CAA84711 (Ref. 1) Curated1
Sequence conflicti97 – 99AAV → GGF in CAA84711 (Ref. 1) Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z35652 Genomic DNA. Translation: CAA84711.1.
AF027868 Genomic DNA. Translation: AAB84426.1.
AL009126 Genomic DNA. Translation: CAB13772.1.
PIRiG69674.
RefSeqiNP_389761.1. NC_000964.3.
WP_003231385.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB13772; CAB13772; BSU18800.
GeneIDi940129.
KEGGibsu:BSU18800.
PATRICi18975601. VBIBacSub10457_1991.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z35652 Genomic DNA. Translation: CAA84711.1.
AF027868 Genomic DNA. Translation: AAB84426.1.
AL009126 Genomic DNA. Translation: CAB13772.1.
PIRiG69674.
RefSeqiNP_389761.1. NC_000964.3.
WP_003231385.1. NZ_JNCM01000036.1.

3D structure databases

ProteinModelPortaliP39824.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100010361.

Proteomic databases

PaxDbiP39824.
PRIDEiP39824.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13772; CAB13772; BSU18800.
GeneIDi940129.
KEGGibsu:BSU18800.
PATRICi18975601. VBIBacSub10457_1991.

Phylogenomic databases

eggNOGiENOG4108J4B. Bacteria.
COG2367. LUCA.
HOGENOMiHOG000201073.
InParanoidiP39824.
KOiK17836.
OMAiDEKEATY.
PhylomeDBiP39824.

Enzyme and pathway databases

BioCyciBSUB:BSU18800-MONOMER.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBLAC_BACSU
AccessioniPrimary (citable) accession number: P39824
Secondary accession number(s): O34848
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: May 30, 2000
Last modified: October 5, 2016
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.