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P39824

- BLAC_BACSU

UniProt

P39824 - BLAC_BACSU

Protein

Beta-lactamase

Gene

penP

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 2 (30 May 2000)
      Previous versions | rss
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    Functioni

    This protein is a beta-lactamase with a substrate specificity for penicillins.

    Catalytic activityi

    A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei89 – 891Acyl-ester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. beta-lactamase activity Source: UniProtKB-EC

    GO - Biological processi

    1. beta-lactam antibiotic catabolic process Source: InterPro
    2. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antibiotic resistance

    Enzyme and pathway databases

    BioCyciBSUB:BSU18800-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-lactamase (EC:3.5.2.6)
    Alternative name(s):
    Penicillinase
    Gene namesi
    Name:penP
    Ordered Locus Names:BSU18800
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU18800. [Micado]

    Subcellular locationi

    Secreted 1 Publication

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 36361 PublicationAdd
    BLAST
    Chaini37 – 306270Beta-lactamasePRO_0000016975Add
    BLAST

    Proteomic databases

    PaxDbiP39824.

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.BSU18800.

    Structurei

    3D structure databases

    ProteinModelPortaliP39824.
    SMRiP39824. Positions 53-305.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni251 – 2533Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the class-A beta-lactamase family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2367.
    HOGENOMiHOG000201073.
    KOiK17836.
    OMAiMKLIGGP.
    OrthoDBiEOG6K9QFS.
    PhylomeDBiP39824.

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR001466. Beta-lactam-related.
    IPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view]
    PfamiPF00144. Beta-lactamase. 1 hit.
    [Graphical view]
    PRINTSiPR00118. BLACTAMASEA.
    SUPFAMiSSF56601. SSF56601. 1 hit.
    PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P39824-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLKTKASIK FGICVGLLCL SITGFTPFFN STHAEAKSIE DTNMASCITN    50
    KKFVQLEKKF DARLGVYAID IGSNKTIAYR PNERFAYAST YKVLAAAAVL 100
    KKNSIEKLNE VIHYSKDDLV TYSPITEKHL DTGMSLKEIS EAAIRYSDNT 150
    AGNILLQQLG GPKGFEKSLK QIGDHVTKAK RFETDLNSAI PGDIRDTSTA 200
    KALATDLKAF TLDNTLTTDK RMILTDWMRG NATGDELIRA GAPIGWEVGD 250
    KSGAGSYGTR NDIAIVWPPN RAPIVVAILS NRFTKDANYD NALIAEAAKV 300
    VLNDLK 306
    Length:306
    Mass (Da):33,446
    Last modified:May 30, 2000 - v2
    Checksum:i95BE5644B731500E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti51 – 511K → N in CAA84711. 1 PublicationCurated
    Sequence conflicti97 – 993AAV → GGF in CAA84711. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z35652 Genomic DNA. Translation: CAA84711.1.
    AF027868 Genomic DNA. Translation: AAB84426.1.
    AL009126 Genomic DNA. Translation: CAB13772.1.
    PIRiG69674.
    RefSeqiNP_389761.1. NC_000964.3.
    WP_003231385.1. NC_000964.3.

    Genome annotation databases

    EnsemblBacteriaiCAB13772; CAB13772; BSU18800.
    GeneIDi940129.
    KEGGibsu:BSU18800.
    PATRICi18975601. VBIBacSub10457_1991.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z35652 Genomic DNA. Translation: CAA84711.1 .
    AF027868 Genomic DNA. Translation: AAB84426.1 .
    AL009126 Genomic DNA. Translation: CAB13772.1 .
    PIRi G69674.
    RefSeqi NP_389761.1. NC_000964.3.
    WP_003231385.1. NC_000964.3.

    3D structure databases

    ProteinModelPortali P39824.
    SMRi P39824. Positions 53-305.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU18800.

    Proteomic databases

    PaxDbi P39824.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB13772 ; CAB13772 ; BSU18800 .
    GeneIDi 940129.
    KEGGi bsu:BSU18800.
    PATRICi 18975601. VBIBacSub10457_1991.

    Organism-specific databases

    GenoListi BSU18800. [Micado ]

    Phylogenomic databases

    eggNOGi COG2367.
    HOGENOMi HOG000201073.
    KOi K17836.
    OMAi MKLIGGP.
    OrthoDBi EOG6K9QFS.
    PhylomeDBi P39824.

    Enzyme and pathway databases

    BioCyci BSUB:BSU18800-MONOMER.

    Family and domain databases

    Gene3Di 3.40.710.10. 1 hit.
    InterProi IPR001466. Beta-lactam-related.
    IPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view ]
    Pfami PF00144. Beta-lactamase. 1 hit.
    [Graphical view ]
    PRINTSi PR00118. BLACTAMASEA.
    SUPFAMi SSF56601. SSF56601. 1 hit.
    PROSITEi PS00146. BETA_LACTAMASE_A. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of penicillinase-encoding genes of Bacillus amyloliquefaciens and Bacillus subtilis."
      van Dijl J.M., de Jong A., Nauta A., Venema G., Bron S.
      Submitted (AUG-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168 / 6GM.
    2. "Sequence analysis of the Bacillus subtilis chromosome region between the terC and odhAB loci cloned in a yeast artificial chromosome."
      Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.
      Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    4. "Proteome analysis of Bacillus subtilis extracellular proteins: a two-dimensional protein electrophoretic study."
      Hirose I., Sano K., Shioda I., Kumano M., Nakamura K., Yamane K.
      Microbiology 146:65-75(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 37-47, SUBCELLULAR LOCATION.
      Strain: 168.

    Entry informationi

    Entry nameiBLAC_BACSU
    AccessioniPrimary (citable) accession number: P39824
    Secondary accession number(s): O34848
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: May 30, 2000
    Last modified: October 1, 2014
    This is version 97 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3