Reviewed, UniProtKB/Swiss-Prot P39824 (BLAC_BACSU)

Names and origin

Protein names

Recommended name:
    Beta-lactamase
    EC=3.5.2.6
Alternative name(s):
    Penicillinase

Gene names

Name:	penP
Ordered Locus Names:	BSU18800

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This protein is a beta-lactamase with a substrate specificity for penicillins.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Subcellular location	Secreted. Ref.4
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 36	36	Ref.4
	Chain	37 – 306	270	Beta-lactamase		PRO_0000016975
Regions
	Region	251 – 253	3	Substrate binding By similarity
Sites
	Active site	89	1	Acyl-ester intermediate By similarity
Experimental info
	Sequence conflict	51	1	K → N in CAA84711. Ref.1
	Sequence conflict	97 – 99	3	AAV → GGF in CAA84711. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P39824-1 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: 95BE5644B731500E	FASTA	306	33,446
	10 20 30 40 50 60 MKLKTKASIK FGICVGLLCL SITGFTPFFN STHAEAKSIE DTNMASCITN KKFVQLEKKF 70 80 90 100 110 120 DARLGVYAID IGSNKTIAYR PNERFAYAST YKVLAAAAVL KKNSIEKLNE VIHYSKDDLV 130 140 150 160 170 180 TYSPITEKHL DTGMSLKEIS EAAIRYSDNT AGNILLQQLG GPKGFEKSLK QIGDHVTKAK 190 200 210 220 230 240 RFETDLNSAI PGDIRDTSTA KALATDLKAF TLDNTLTTDK RMILTDWMRG NATGDELIRA 250 260 270 280 290 300 GAPIGWEVGD KSGAGSYGTR NDIAIVWPPN RAPIVVAILS NRFTKDANYD NALIAEAAKV VLNDLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of penicillinase-encoding genes of Bacillus amyloliquefaciens and Bacillus subtilis." van Dijl J.M., de Jong A., Nauta A., Venema G., Bron S. Submitted (AUG-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / 6GM.
[2]	"Sequence analysis of the Bacillus subtilis chromosome region between the terC and odhAB loci cloned in a yeast artificial chromosome." Lapidus A., Galleron N., Sorokin A., Ehrlich S.D. Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"Proteome analysis of Bacillus subtilis extracellular proteins: a two-dimensional protein electrophoretic study." Hirose I., Sano K., Shioda I., Kumano M., Nakamura K., Yamane K. Microbiology 146:65-75(2000) [PubMed: 10658653] [Abstract] Cited for: PROTEIN SEQUENCE OF 37-47, SUBCELLULAR LOCATION. Strain: 168.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z35652 Genomic DNA. Translation: CAA84711.1. AF027868 Genomic DNA. Translation: AAB84426.1. AL009126 Genomic DNA. Translation: CAB13772.1.
PIR	G69674.
RefSeq	NP_389761.1.
3D structure databases
HSSP	HSSP built from PDB template 1I2S based on UniProtKB P00808.
ModBase	Search...
Genome annotation databases
GeneID	940129.
GenomeReviews	Gene locus BSU18800 in contig AL009126_GR.
KEGG	bsu:BSU18800.
NMPDR	fig|224308.1.peg.1883.
Organism-specific databases
SubtiList	BG11016. penP. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P39824.
OMA	RVGDKTG.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU1879-MON.
BRENDA	3.5.2.6. 150.
Family and domain databases
InterPro	IPR001466. Beta_lactamase-related. IPR000871. Beta_lactamase_A/D. [Graphical view]
Pfam	PF00144. Beta-lactamase. 1 hit. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
PROSITE	PS00146. BETA_LACTAMASE_A. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLAC_BACSU

Accession

Primary (citable) accession number: P39824
Secondary accession number(s): O34848

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	May 30, 2000
Last modified:	November 3, 2009
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)