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Protein

Beta-lactamase

Gene

penP

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This protein is a beta-lactamase with a substrate specificity for penicillins.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei89 – 891Acyl-ester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. beta-lactamase activity Source: UniProtKB-EC

GO - Biological processi

  1. beta-lactam antibiotic catabolic process Source: InterPro
  2. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Enzyme and pathway databases

BioCyciBSUB:BSU18800-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase (EC:3.5.2.6)
Alternative name(s):
Penicillinase
Gene namesi
Name:penP
Ordered Locus Names:BSU18800
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU18800. [Micado]

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 36361 PublicationAdd
BLAST
Chaini37 – 306270Beta-lactamasePRO_0000016975Add
BLAST

Proteomic databases

PaxDbiP39824.

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU18800.

Structurei

3D structure databases

ProteinModelPortaliP39824.
SMRiP39824. Positions 53-305.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni251 – 2533Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the class-A beta-lactamase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2367.
HOGENOMiHOG000201073.
InParanoidiP39824.
KOiK17836.
OMAiAVIWPPD.
OrthoDBiEOG6K9QFS.
PhylomeDBiP39824.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PfamiPF00144. Beta-lactamase. 1 hit.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P39824-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLKTKASIK FGICVGLLCL SITGFTPFFN STHAEAKSIE DTNMASCITN
60 70 80 90 100
KKFVQLEKKF DARLGVYAID IGSNKTIAYR PNERFAYAST YKVLAAAAVL
110 120 130 140 150
KKNSIEKLNE VIHYSKDDLV TYSPITEKHL DTGMSLKEIS EAAIRYSDNT
160 170 180 190 200
AGNILLQQLG GPKGFEKSLK QIGDHVTKAK RFETDLNSAI PGDIRDTSTA
210 220 230 240 250
KALATDLKAF TLDNTLTTDK RMILTDWMRG NATGDELIRA GAPIGWEVGD
260 270 280 290 300
KSGAGSYGTR NDIAIVWPPN RAPIVVAILS NRFTKDANYD NALIAEAAKV

VLNDLK
Length:306
Mass (Da):33,446
Last modified:May 30, 2000 - v2
Checksum:i95BE5644B731500E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti51 – 511K → N in CAA84711 (Ref. 1) Curated
Sequence conflicti97 – 993AAV → GGF in CAA84711 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z35652 Genomic DNA. Translation: CAA84711.1.
AF027868 Genomic DNA. Translation: AAB84426.1.
AL009126 Genomic DNA. Translation: CAB13772.1.
PIRiG69674.
RefSeqiNP_389761.1. NC_000964.3.
WP_003231385.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB13772; CAB13772; BSU18800.
GeneIDi940129.
KEGGibsu:BSU18800.
PATRICi18975601. VBIBacSub10457_1991.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z35652 Genomic DNA. Translation: CAA84711.1.
AF027868 Genomic DNA. Translation: AAB84426.1.
AL009126 Genomic DNA. Translation: CAB13772.1.
PIRiG69674.
RefSeqiNP_389761.1. NC_000964.3.
WP_003231385.1. NZ_JNCM01000036.1.

3D structure databases

ProteinModelPortaliP39824.
SMRiP39824. Positions 53-305.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.BSU18800.

Proteomic databases

PaxDbiP39824.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13772; CAB13772; BSU18800.
GeneIDi940129.
KEGGibsu:BSU18800.
PATRICi18975601. VBIBacSub10457_1991.

Organism-specific databases

GenoListiBSU18800. [Micado]

Phylogenomic databases

eggNOGiCOG2367.
HOGENOMiHOG000201073.
InParanoidiP39824.
KOiK17836.
OMAiAVIWPPD.
OrthoDBiEOG6K9QFS.
PhylomeDBiP39824.

Enzyme and pathway databases

BioCyciBSUB:BSU18800-MONOMER.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PfamiPF00144. Beta-lactamase. 1 hit.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of penicillinase-encoding genes of Bacillus amyloliquefaciens and Bacillus subtilis."
    van Dijl J.M., de Jong A., Nauta A., Venema G., Bron S.
    Submitted (AUG-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168 / 6GM.
  2. "Sequence analysis of the Bacillus subtilis chromosome region between the terC and odhAB loci cloned in a yeast artificial chromosome."
    Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.
    Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  4. "Proteome analysis of Bacillus subtilis extracellular proteins: a two-dimensional protein electrophoretic study."
    Hirose I., Sano K., Shioda I., Kumano M., Nakamura K., Yamane K.
    Microbiology 146:65-75(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 37-47, SUBCELLULAR LOCATION.
    Strain: 168.

Entry informationi

Entry nameiBLAC_BACSU
AccessioniPrimary (citable) accession number: P39824
Secondary accession number(s): O34848
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: May 30, 2000
Last modified: January 7, 2015
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.