P39824 (BLAC_BACSU) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 90.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Beta-lactamase EC=3.5.2.6 Alternative name(s): Penicillinase | ||||
| Gene names |
| ||||
| Organism | Bacillus subtilis (strain 168) [Reference proteome] [HAMAP] | ||||
| Taxonomic identifier | 224308 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus ›  |
Protein attributes
| Sequence length | 306 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | This protein is a beta-lactamase with a substrate specificity for penicillins. |
| Catalytic activity | A beta-lactam + H2O = a substituted beta-amino acid. |
| Subcellular location | |
| Sequence similarities | Belongs to the class-A beta-lactamase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Antibiotic resistance |
| Cellular component | Secreted |
| Domain | Signal |
| Molecular function | Hydrolase |
| Technical term | Complete proteome Direct protein sequencing Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibioticInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular_component | extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular_function | beta-lactamase activity Inferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 36 | 36 | Ref.4 | ||||||
| Chain | 37 – 306 | 270 | Beta-lactamase | PRO_0000016975 | |||||
Regions | |||||||||
| Region | 251 – 253 | 3 | Substrate binding By similarity | ||||||
Sites | |||||||||
| Active site | 89 | 1 | Acyl-ester intermediate By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 51 | 1 | K → N in CAA84711. Ref.1 | ||||||
| Sequence conflict | 97 – 99 | 3 | AAV → GGF in CAA84711. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Identification of penicillinase-encoding genes of Bacillus amyloliquefaciens and Bacillus subtilis." van Dijl J.M., de Jong A., Nauta A., Venema G., Bron S. Submitted (AUG-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / 6GM. |
| [2] | "Sequence analysis of the Bacillus subtilis chromosome region between the terC and odhAB loci cloned in a yeast artificial chromosome." Lapidus A., Galleron N., Sorokin A., Ehrlich S.D. Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [3] | "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.  Danchin A.Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168. |
| [4] | "Proteome analysis of Bacillus subtilis extracellular proteins: a two-dimensional protein electrophoretic study." Hirose I., Sano K., Shioda I., Kumano M., Nakamura K., Yamane K. Microbiology 146:65-75(2000) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 37-47, SUBCELLULAR LOCATION. Strain: 168. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | Z35652 Genomic DNA. Translation: CAA84711.1. AF027868 Genomic DNA. Translation: AAB84426.1. AL009126 Genomic DNA. Translation: CAB13772.1. |
| PIR | G69674. |
| RefSeq | NP_389761.1. NC_000964.3. |
3D structure databases | |
| ProteinModelPortal | P39824. |
| SMR | P39824. Positions 53-305. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | 224308.BSU18800. |
Proteomic databases | |
| PaxDb | P39824. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblBacteria | CAB13772; CAB13772; BSU18800. |
| GeneID | 940129. |
| KEGG | bsu:BSU18800. |
| PATRIC | 18975601. VBIBacSub10457_1991. |
Organism-specific databases | |
| GenoList | BSU18800. [Micado] |
Phylogenomic databases | |
| eggNOG | COG2367. |
| HOGENOM | HOG000201073. |
| KO | K01467. |
| OMA | DLVNYSP. |
| ProtClustDB | CLSK2393383. |
Enzyme and pathway databases | |
| BioCyc | BSUB:BSU18800-MONOMER. |
Family and domain databases | |
| Gene3D | 3.40.710.10. 1 hit. |
| InterPro | IPR001466. Beta-lactam-related. IPR012338. Beta-lactam/transpept-like. IPR000871. Beta-lactam_class-A/D. IPR023650. Beta-lactam_class-A_AS. [Graphical view] |
| Pfam | PF00144. Beta-lactamase. 1 hit. [Graphical view] |
| PRINTS | PR00118. BLACTAMASEA. |
| SUPFAM | SSF56601. PBP_transp_fold. 1 hit. |
| PROSITE | PS00146. BETA_LACTAMASE_A. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | BLAC_BACSU | ||||||||
| Accession | Primary (citable) accession number: P39824 Secondary accession number(s): O34848 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| Bacillus subtilis Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList |
| SIMILARITY comments Index of protein domains and families |