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P39820

- PROB_BACSU

UniProt

P39820 - PROB_BACSU

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Protein

Glutamate 5-kinase 1

Gene

proB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei9 – 91ATPUniRule annotation
Binding sitei49 – 491SubstrateUniRule annotation
Binding sitei136 – 1361SubstrateUniRule annotation
Binding sitei148 – 1481Substrate; via amide nitrogenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi168 – 1692ATPUniRule annotation
Nucleotide bindingi210 – 2167ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate 5-kinase activity Source: UniProtKB-HAMAP
  3. RNA binding Source: InterPro

GO - Biological processi

  1. L-proline biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU13120-MONOMER.
UniPathwayiUPA00098; UER00359.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinase 1UniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinase 1UniRule annotation
Short name:
GK 1UniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:BSU13120
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU13120. [Micado]

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 365365Glutamate 5-kinase 1PRO_0000109641Add
BLAST

Proteomic databases

PaxDbiP39820.

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU13120.

Structurei

3D structure databases

ProteinModelPortaliP39820.
SMRiP39820. Positions 2-362.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini276 – 35378PUAUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation
Contains 1 PUA domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0263.
HOGENOMiHOG000246369.
InParanoidiP39820.
KOiK00931.
OrthoDBiEOG6PGK7G.
PhylomeDBiP39820.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view]
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SMARTiSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39820-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKKQRIVVKI GSSSLTNSKG SIDEAKIREH VQAISVLKKA GHEMILITSG
60 70 80 90 100
AVAAGFSSLG YPSRPVTIKG KQAAAAVGQT LLMQQYMNQF KQYSLTPGQI
110 120 130 140 150
LLTRNDFSKR ERYRNAYATI MELLERGVIP IINENDSTSV EELTFGDNDM
160 170 180 190 200
LSALVSGLIH ADQLMILTDI NGLYDANPNE NPEAKRFDYL PEITPELLGY
210 220 230 240 250
AGSAGSKVGT GGMKSKLLAT QTALSLGVKV FIGTGSGEQK LADILDGRGD
260 270 280 290 300
GTYIGDKELS SVNNTRQWIQ FHSPISGEII IDAGAEEAMI HNGSSLLPAG
310 320 330 340 350
VVGVNGSFPK GAVVEVRGPG GVIGKGQTHY SSEEIMEAKG KRSDELDFEK
360
TFEVIHRNDW VNVKD
Length:365
Mass (Da):39,346
Last modified:June 16, 2009 - v3
Checksum:iD5B8DB471ADE62E2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti25 – 328AKIREHVQ → QNQRACS in BAA05044. (PubMed:8083159)Curated
Sequence conflicti51 – 511A → P in CAA05591. 1 PublicationCurated
Sequence conflicti54 – 541A → R in CAA05591. 1 PublicationCurated
Sequence conflicti181 – 1855NPEAK → ILSE in BAA05044. (PubMed:8083159)Curated
Sequence conflicti357 – 3659Missing in BAA05044. (PubMed:8083159)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D26044 Genomic DNA. Translation: BAA05044.1.
AJ002571 Genomic DNA. Translation: CAA05591.1.
AL009126 Genomic DNA. Translation: CAB13169.2.
PIRiD69682.
RefSeqiNP_389195.2. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB13169; CAB13169; BSU13120.
GeneIDi936790.
KEGGibsu:BSU13120.
PATRICi18974381. VBIBacSub10457_1383.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D26044 Genomic DNA. Translation: BAA05044.1 .
AJ002571 Genomic DNA. Translation: CAA05591.1 .
AL009126 Genomic DNA. Translation: CAB13169.2 .
PIRi D69682.
RefSeqi NP_389195.2. NC_000964.3.

3D structure databases

ProteinModelPortali P39820.
SMRi P39820. Positions 2-362.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224308.BSU13120.

Proteomic databases

PaxDbi P39820.

Protocols and materials databases

DNASUi 936790.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB13169 ; CAB13169 ; BSU13120 .
GeneIDi 936790.
KEGGi bsu:BSU13120.
PATRICi 18974381. VBIBacSub10457_1383.

Organism-specific databases

GenoListi BSU13120. [Micado ]

Phylogenomic databases

eggNOGi COG0263.
HOGENOMi HOG000246369.
InParanoidi P39820.
KOi K00931.
OrthoDBi EOG6PGK7G.
PhylomeDBi P39820.

Enzyme and pathway databases

UniPathwayi UPA00098 ; UER00359 .
BioCyci BSUB:BSU13120-MONOMER.

Family and domain databases

Gene3Di 2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPi MF_00456. ProB.
InterProi IPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view ]
Pfami PF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view ]
PIRSFi PIRSF000729. GK. 1 hit.
PRINTSi PR00474. GLU5KINASE.
SMARTi SM00359. PUA. 1 hit.
[Graphical view ]
SUPFAMi SSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsi TIGR01027. proB. 1 hit.
PROSITEi PS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Multiple copies of the proB gene enhance degS-dependent extracellular protease production in Bacillus subtilis."
    Ogura M., Kawata-Mukai M., Itaya M., Takio K., Tanaka T.
    J. Bacteriol. 176:5673-5680(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-20.
    Strain: 168.
  2. "Sequence of the Bacillus subtilis genome between xlyA and ykoR."
    Devine K.M.
    Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  4. "From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
    Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
    Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO 51 AND 54.

Entry informationi

Entry nameiPROB_BACSU
AccessioniPrimary (citable) accession number: P39820
Secondary accession number(s): O34562
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: June 16, 2009
Last modified: October 29, 2014
This is version 110 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3