Reviewed, UniProtKB/Swiss-Prot P39820 (PROB_BACSU)

Names and origin

Protein names

Recommended name:
    Glutamate 5-kinase 1
    EC=2.7.2.11
Alternative name(s):
    Gamma-glutamyl kinase 1
      Short name=GK 1

Gene names

Name:	proB
Ordered Locus Names:	BSU13120

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline. HAMAP MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP MF_00456
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 365	365	Glutamate 5-kinase 1 HAMAP MF_00456		PRO_0000109641
Regions
	Domain	276 – 353	78	PUA
Experimental info
	Sequence conflict	25 – 32	8	AKIREHVQ → QNQRACS in BAA05044. Ref.1
	Sequence conflict	51	1	A → P in CAA05591. Ref.2
	Sequence conflict	54	1	A → R in CAA05591. Ref.2
	Sequence conflict	181 – 185	5	NPEAK → ILSE in BAA05044. Ref.1
	Sequence conflict	357 – 365	9	Missing in BAA05044. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P39820-1 [UniParc]. Last modified June 16, 2009. Version 3. Checksum: D5B8DB471ADE62E2	FASTA	365	39,346
	10 20 30 40 50 60 MKKQRIVVKI GSSSLTNSKG SIDEAKIREH VQAISVLKKA GHEMILITSG AVAAGFSSLG 70 80 90 100 110 120 YPSRPVTIKG KQAAAAVGQT LLMQQYMNQF KQYSLTPGQI LLTRNDFSKR ERYRNAYATI 130 140 150 160 170 180 MELLERGVIP IINENDSTSV EELTFGDNDM LSALVSGLIH ADQLMILTDI NGLYDANPNE 190 200 210 220 230 240 NPEAKRFDYL PEITPELLGY AGSAGSKVGT GGMKSKLLAT QTALSLGVKV FIGTGSGEQK 250 260 270 280 290 300 LADILDGRGD GTYIGDKELS SVNNTRQWIQ FHSPISGEII IDAGAEEAMI HNGSSLLPAG 310 320 330 340 350 360 VVGVNGSFPK GAVVEVRGPG GVIGKGQTHY SSEEIMEAKG KRSDELDFEK TFEVIHRNDW VNVKD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Multiple copies of the proB gene enhance degS-dependent extracellular protease production in Bacillus subtilis." Ogura M., Kawata-Mukai M., Itaya M., Takio K., Tanaka T. J. Bacteriol. 176:5673-5680(1994) [PubMed: 8083159] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-20. Strain: 168.
[2]	"Sequence of the Bacillus subtilis genome between xlyA and ykoR." Devine K.M. Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract] Cited for: SEQUENCE REVISION TO 51 AND 54.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D26044 Genomic DNA. Translation: BAA05044.1. AJ002571 Genomic DNA. Translation: CAA05591.1. AL009126 Genomic DNA. Translation: CAB13169.2.
PIR	D69682.
RefSeq	NP_389195.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	936790.
GenomeReviews	Gene locus BSU13120 in contig AL009126_GR.
KEGG	bsu:BSU13120.
NMPDR	fig|224308.1.peg.1314.
Organism-specific databases
SubtiList	BG10963. proB. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P39820.
OMA	ILTDQRG.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU1314-MON.
BRENDA	2.7.2.11. 150.
Family and domain databases
HAMAP	MF_00456. [Tree]
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu_5kinase. IPR011529. Glu_5kinase_bact. IPR019797. Glutamate_5-kinase_CS. IPR005715. ProB. IPR002478. PUA. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SMART	SM00359. PUA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_BACSU

Accession

Primary (citable) accession number: P39820
Secondary accession number(s): O34562

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	June 16, 2009
Last modified:	November 3, 2009
This is version 71 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)