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Protein

Glutamate 5-kinase 1

Gene

proB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase 2 (proJ), Glutamate 5-kinase 1 (proB)
  2. Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei9ATPUniRule annotation1
Binding sitei49SubstrateUniRule annotation1
Binding sitei136SubstrateUniRule annotation1
Binding sitei148Substrate; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi168 – 169ATPUniRule annotation2
Nucleotide bindingi210 – 216ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU13120-MONOMER.
UniPathwayiUPA00098; UER00359.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinase 1UniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinase 1UniRule annotation
Short name:
GK 1UniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:BSU13120
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001096411 – 365Glutamate 5-kinase 1Add BLAST365

Proteomic databases

PaxDbiP39820.

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100007266.

Structurei

3D structure databases

ProteinModelPortaliP39820.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini276 – 353PUAUniRule annotationAdd BLAST78

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation
Contains 1 PUA domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CGT. Bacteria.
COG0263. LUCA.
HOGENOMiHOG000246369.
InParanoidiP39820.
KOiK00931.
OMAiHDYGMRC.
PhylomeDBiP39820.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB. 1 hit.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view]
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SMARTiSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39820-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKQRIVVKI GSSSLTNSKG SIDEAKIREH VQAISVLKKA GHEMILITSG
60 70 80 90 100
AVAAGFSSLG YPSRPVTIKG KQAAAAVGQT LLMQQYMNQF KQYSLTPGQI
110 120 130 140 150
LLTRNDFSKR ERYRNAYATI MELLERGVIP IINENDSTSV EELTFGDNDM
160 170 180 190 200
LSALVSGLIH ADQLMILTDI NGLYDANPNE NPEAKRFDYL PEITPELLGY
210 220 230 240 250
AGSAGSKVGT GGMKSKLLAT QTALSLGVKV FIGTGSGEQK LADILDGRGD
260 270 280 290 300
GTYIGDKELS SVNNTRQWIQ FHSPISGEII IDAGAEEAMI HNGSSLLPAG
310 320 330 340 350
VVGVNGSFPK GAVVEVRGPG GVIGKGQTHY SSEEIMEAKG KRSDELDFEK
360
TFEVIHRNDW VNVKD
Length:365
Mass (Da):39,346
Last modified:June 16, 2009 - v3
Checksum:iD5B8DB471ADE62E2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti25 – 32AKIREHVQ → QNQRACS in BAA05044 (PubMed:8083159).Curated8
Sequence conflicti51A → P in CAA05591 (Ref. 2) Curated1
Sequence conflicti54A → R in CAA05591 (Ref. 2) Curated1
Sequence conflicti181 – 185NPEAK → ILSE in BAA05044 (PubMed:8083159).Curated5
Sequence conflicti357 – 365Missing in BAA05044 (PubMed:8083159).Curated9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26044 Genomic DNA. Translation: BAA05044.1.
AJ002571 Genomic DNA. Translation: CAA05591.1.
AL009126 Genomic DNA. Translation: CAB13169.2.
PIRiD69682.
RefSeqiNP_389195.2. NC_000964.3.
WP_003244794.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB13169; CAB13169; BSU13120.
GeneIDi936790.
KEGGibsu:BSU13120.
PATRICi18974381. VBIBacSub10457_1383.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26044 Genomic DNA. Translation: BAA05044.1.
AJ002571 Genomic DNA. Translation: CAA05591.1.
AL009126 Genomic DNA. Translation: CAB13169.2.
PIRiD69682.
RefSeqiNP_389195.2. NC_000964.3.
WP_003244794.1. NZ_JNCM01000035.1.

3D structure databases

ProteinModelPortaliP39820.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100007266.

Proteomic databases

PaxDbiP39820.

Protocols and materials databases

DNASUi936790.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13169; CAB13169; BSU13120.
GeneIDi936790.
KEGGibsu:BSU13120.
PATRICi18974381. VBIBacSub10457_1383.

Phylogenomic databases

eggNOGiENOG4105CGT. Bacteria.
COG0263. LUCA.
HOGENOMiHOG000246369.
InParanoidiP39820.
KOiK00931.
OMAiHDYGMRC.
PhylomeDBiP39820.

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359.
BioCyciBSUB:BSU13120-MONOMER.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB. 1 hit.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view]
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SMARTiSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPROB_BACSU
AccessioniPrimary (citable) accession number: P39820
Secondary accession number(s): O34562
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: June 16, 2009
Last modified: October 5, 2016
This is version 120 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.