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P39820

- PROB_BACSU

UniProt

P39820 - PROB_BACSU

Protein

Glutamate 5-kinase 1

Gene

proB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 3 (16 Jun 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

    Catalytic activityi

    ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei9 – 91ATPUniRule annotation
    Binding sitei49 – 491SubstrateUniRule annotation
    Binding sitei136 – 1361SubstrateUniRule annotation
    Binding sitei148 – 1481Substrate; via amide nitrogenUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi168 – 1692ATPUniRule annotation
    Nucleotide bindingi210 – 2167ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate 5-kinase activity Source: UniProtKB-HAMAP
    3. RNA binding Source: InterPro

    GO - Biological processi

    1. L-proline biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Proline biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBSUB:BSU13120-MONOMER.
    UniPathwayiUPA00098; UER00359.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate 5-kinase 1UniRule annotation (EC:2.7.2.11UniRule annotation)
    Alternative name(s):
    Gamma-glutamyl kinase 1UniRule annotation
    Short name:
    GK 1UniRule annotation
    Gene namesi
    Name:proBUniRule annotation
    Ordered Locus Names:BSU13120
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU13120. [Micado]

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 365365Glutamate 5-kinase 1PRO_0000109641Add
    BLAST

    Proteomic databases

    PaxDbiP39820.

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.BSU13120.

    Structurei

    3D structure databases

    ProteinModelPortaliP39820.
    SMRiP39820. Positions 2-362.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini276 – 35378PUAUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glutamate 5-kinase family.UniRule annotation
    Contains 1 PUA domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0263.
    HOGENOMiHOG000246369.
    KOiK00931.
    OrthoDBiEOG6PGK7G.
    PhylomeDBiP39820.

    Family and domain databases

    Gene3Di2.30.130.10. 1 hit.
    3.40.1160.10. 1 hit.
    HAMAPiMF_00456. ProB.
    InterProiIPR001048. Asp/Glu/Uridylate_kinase.
    IPR001057. Glu/AcGlu_kinase.
    IPR011529. Glu_5kinase.
    IPR005715. Glu_5kinase/COase_Synthase.
    IPR019797. Glutamate_5-kinase_CS.
    IPR002478. PUA.
    IPR015947. PUA-like_domain.
    [Graphical view]
    PfamiPF00696. AA_kinase. 1 hit.
    PF01472. PUA. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000729. GK. 1 hit.
    PRINTSiPR00474. GLU5KINASE.
    SMARTiSM00359. PUA. 1 hit.
    [Graphical view]
    SUPFAMiSSF53633. SSF53633. 1 hit.
    SSF88697. SSF88697. 1 hit.
    TIGRFAMsiTIGR01027. proB. 1 hit.
    PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
    PS50890. PUA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P39820-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKKQRIVVKI GSSSLTNSKG SIDEAKIREH VQAISVLKKA GHEMILITSG    50
    AVAAGFSSLG YPSRPVTIKG KQAAAAVGQT LLMQQYMNQF KQYSLTPGQI 100
    LLTRNDFSKR ERYRNAYATI MELLERGVIP IINENDSTSV EELTFGDNDM 150
    LSALVSGLIH ADQLMILTDI NGLYDANPNE NPEAKRFDYL PEITPELLGY 200
    AGSAGSKVGT GGMKSKLLAT QTALSLGVKV FIGTGSGEQK LADILDGRGD 250
    GTYIGDKELS SVNNTRQWIQ FHSPISGEII IDAGAEEAMI HNGSSLLPAG 300
    VVGVNGSFPK GAVVEVRGPG GVIGKGQTHY SSEEIMEAKG KRSDELDFEK 350
    TFEVIHRNDW VNVKD 365
    Length:365
    Mass (Da):39,346
    Last modified:June 16, 2009 - v3
    Checksum:iD5B8DB471ADE62E2
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti25 – 328AKIREHVQ → QNQRACS in BAA05044. (PubMed:8083159)Curated
    Sequence conflicti51 – 511A → P in CAA05591. 1 PublicationCurated
    Sequence conflicti54 – 541A → R in CAA05591. 1 PublicationCurated
    Sequence conflicti181 – 1855NPEAK → ILSE in BAA05044. (PubMed:8083159)Curated
    Sequence conflicti357 – 3659Missing in BAA05044. (PubMed:8083159)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D26044 Genomic DNA. Translation: BAA05044.1.
    AJ002571 Genomic DNA. Translation: CAA05591.1.
    AL009126 Genomic DNA. Translation: CAB13169.2.
    PIRiD69682.
    RefSeqiNP_389195.2. NC_000964.3.

    Genome annotation databases

    EnsemblBacteriaiCAB13169; CAB13169; BSU13120.
    GeneIDi936790.
    KEGGibsu:BSU13120.
    PATRICi18974381. VBIBacSub10457_1383.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D26044 Genomic DNA. Translation: BAA05044.1 .
    AJ002571 Genomic DNA. Translation: CAA05591.1 .
    AL009126 Genomic DNA. Translation: CAB13169.2 .
    PIRi D69682.
    RefSeqi NP_389195.2. NC_000964.3.

    3D structure databases

    ProteinModelPortali P39820.
    SMRi P39820. Positions 2-362.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU13120.

    Proteomic databases

    PaxDbi P39820.

    Protocols and materials databases

    DNASUi 936790.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB13169 ; CAB13169 ; BSU13120 .
    GeneIDi 936790.
    KEGGi bsu:BSU13120.
    PATRICi 18974381. VBIBacSub10457_1383.

    Organism-specific databases

    GenoListi BSU13120. [Micado ]

    Phylogenomic databases

    eggNOGi COG0263.
    HOGENOMi HOG000246369.
    KOi K00931.
    OrthoDBi EOG6PGK7G.
    PhylomeDBi P39820.

    Enzyme and pathway databases

    UniPathwayi UPA00098 ; UER00359 .
    BioCyci BSUB:BSU13120-MONOMER.

    Family and domain databases

    Gene3Di 2.30.130.10. 1 hit.
    3.40.1160.10. 1 hit.
    HAMAPi MF_00456. ProB.
    InterProi IPR001048. Asp/Glu/Uridylate_kinase.
    IPR001057. Glu/AcGlu_kinase.
    IPR011529. Glu_5kinase.
    IPR005715. Glu_5kinase/COase_Synthase.
    IPR019797. Glutamate_5-kinase_CS.
    IPR002478. PUA.
    IPR015947. PUA-like_domain.
    [Graphical view ]
    Pfami PF00696. AA_kinase. 1 hit.
    PF01472. PUA. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000729. GK. 1 hit.
    PRINTSi PR00474. GLU5KINASE.
    SMARTi SM00359. PUA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53633. SSF53633. 1 hit.
    SSF88697. SSF88697. 1 hit.
    TIGRFAMsi TIGR01027. proB. 1 hit.
    PROSITEi PS00902. GLUTAMATE_5_KINASE. 1 hit.
    PS50890. PUA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Multiple copies of the proB gene enhance degS-dependent extracellular protease production in Bacillus subtilis."
      Ogura M., Kawata-Mukai M., Itaya M., Takio K., Tanaka T.
      J. Bacteriol. 176:5673-5680(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-20.
      Strain: 168.
    2. "Sequence of the Bacillus subtilis genome between xlyA and ykoR."
      Devine K.M.
      Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    4. "From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
      Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
      Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: SEQUENCE REVISION TO 51 AND 54.

    Entry informationi

    Entry nameiPROB_BACSU
    AccessioniPrimary (citable) accession number: P39820
    Secondary accession number(s): O34562
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: June 16, 2009
    Last modified: October 1, 2014
    This is version 109 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3