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Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

trmFO

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.2 Publications

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.

Cofactori

FAD1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 156FADBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciBSUB:BSU16130-MONOMER.
BRENDAi2.1.1.74. 658.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO (EC:2.1.1.74)
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferase
Folate-dependent tRNA(M-5-U54)-methyltransferase
Gene namesi
Name:trmFO
Synonyms:gid, ylyC
Ordered Locus Names:BSU16130
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 435435Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOPRO_0000117234Add
BLAST

Proteomic databases

PaxDbiP39815.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100008886.

Structurei

3D structure databases

ProteinModelPortaliP39815.
SMRiP39815. Positions 1-435.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family. TrmFO subfamily.Curated

Phylogenomic databases

eggNOGiENOG4107QXI. Bacteria.
COG1206. LUCA.
HOGENOMiHOG000252054.
InParanoidiP39815.
KOiK04094.
OMAiDYLNCPM.
PhylomeDBiP39815.

Family and domain databases

Gene3Di3.50.50.60. 3 hits.
HAMAPiMF_01037. TrmFO. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39815-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQQTVNVIG AGLAGSEAAW QLAKRGIQVK LYEMRPVKQT PAHHTDKFAE
60 70 80 90 100
LVCSNSLRSN TLANAVGVLK EEMRALDSAI IAAADECSVP AGGALAVDRH
110 120 130 140 150
EFAASVTNRV KNHPNVTVIN EEVTEIPEGP TIIATGPLTS ESLSAQLKEL
160 170 180 190 200
TGEDYLYFYD AAAPIVEKDS LDMDKVYLKS RYDKGEAAYL NCPMTEEEFD
210 220 230 240 250
RFHEALTSAE TVPLKEFEKE IFFEGCMPIE VMAKRGKKTM LFGPMKPVGL
260 270 280 290 300
EHPVTGKRPY AVVQLRQDDA AGTLYNIVGF QTHLKWGDQK EVLKLIPGLE
310 320 330 340 350
NVEIVRYGVM HRNTFINSPS LLKPTYQFKN RSDLFFAGQM TGVEGYVESA
360 370 380 390 400
ASGLVAGINA AKLVLGEELV IFPQETAIGS MAHYITTTNQ KNFQPMNANF
410 420 430
GLLKELPVKI KNKKERNEQY ANRAIETIQT ISKTI
Length:435
Mass (Da):48,063
Last modified:July 15, 1998 - v2
Checksum:i6C7AB028F484B683
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ000975 Genomic DNA. Translation: CAA04423.1.
AL009126 Genomic DNA. Translation: CAB13486.1.
L27797 Genomic DNA. Translation: AAA22764.1.
U13634 Genomic DNA. Translation: AAB03368.1.
PIRiA69632.
RefSeqiNP_389495.1. NC_000964.3.
WP_003244725.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB13486; CAB13486; BSU16130.
GeneIDi938816.
KEGGibsu:BSU16130.
PATRICi18975031. VBIBacSub10457_1707.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ000975 Genomic DNA. Translation: CAA04423.1.
AL009126 Genomic DNA. Translation: CAB13486.1.
L27797 Genomic DNA. Translation: AAA22764.1.
U13634 Genomic DNA. Translation: AAB03368.1.
PIRiA69632.
RefSeqiNP_389495.1. NC_000964.3.
WP_003244725.1. NZ_JNCM01000035.1.

3D structure databases

ProteinModelPortaliP39815.
SMRiP39815. Positions 1-435.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100008886.

Proteomic databases

PaxDbiP39815.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13486; CAB13486; BSU16130.
GeneIDi938816.
KEGGibsu:BSU16130.
PATRICi18975031. VBIBacSub10457_1707.

Phylogenomic databases

eggNOGiENOG4107QXI. Bacteria.
COG1206. LUCA.
HOGENOMiHOG000252054.
InParanoidiP39815.
KOiK04094.
OMAiDYLNCPM.
PhylomeDBiP39815.

Enzyme and pathway databases

BioCyciBSUB:BSU16130-MONOMER.
BRENDAi2.1.1.74. 658.

Family and domain databases

Gene3Di3.50.50.60. 3 hits.
HAMAPiMF_01037. TrmFO. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMFO_BACSU
AccessioniPrimary (citable) accession number: P39815
Secondary accession number(s): Q6LCY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 15, 1998
Last modified: September 7, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.