Reviewed, UniProtKB/Swiss-Prot P39814 (TOP1_BACSU)

Names and origin

Protein names

Recommended name:
    DNA topoisomerase 1
    EC=5.99.1.2
Alternative name(s):
    DNA topoisomerase I
    Omega-protein
    Relaxing enzyme
    Untwisting enzyme
    Swivelase

Gene names

Name:	topA
Synonyms:	topI
Ordered Locus Names:	BSU16120

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	691 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Subunit structure	Monomer By similarity.
Miscellaneous	When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand.
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family.

Ontologies

Keywords
Domain	Repeat Zinc-finger
Ligand	ATP-binding DNA-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA unwinding during replication Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 691	691	DNA topoisomerase 1		PRO_0000145142
Regions
	Zinc finger	579 – 605	27	C4-type 1
	Zinc finger	619 – 647	29	C4-type 2
	Zinc finger	660 – 683	24	C4-type 3
Sites
	Active site	298	1	For DNA cleavage activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	P39814-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 58A45C84E52711BB	FASTA	691	79,078
	10 20 30 40 50 60 MSDYLVIVES PAKAKTIERY LGKKYKVKAS MGHVRDLPKS QMGVDIEQNF EPKYITIRGK 70 80 90 100 110 120 GPVLKELKTA AKKAKKVYLA ADPDREGEAI AWHLAHSLDL DLNSDCRVVF NEITKDAIKE 130 140 150 160 170 180 SFKHPRMINM DLVDAQQARR ILDRLVGYKI SPILWKKVKK GLSAGRVQSV ALRLIIDREK 190 200 210 220 230 240 EINDFKPEEY WTIDGTFLKG QETFEASFFG KNGKKLPLNS EADVKEILSQ LKGNQYTVEK 250 260 270 280 290 300 VTKKERKRNP ALPFTTSTLQ QEAARKLNFR AKKTMMIAQQ LYEGIDLGRE GTVGLITYMR 310 320 330 340 350 360 TDSTRISNTA VDEAAAFIDQ TYGKEFLGGK RKPAKKNENA QDAHEAIRPT SVLRKPSELK 370 380 390 400 410 420 AVLGRDQMRL YKLIWERFVA SQMAPAVLDT MSVDLTNNGL TFRANGSKVK FSGFMKVYVE 430 440 450 460 470 480 GKDDQMEEKD RMLPDLQEGD TVLSKDIEPE QHFTQPPPRY TEARLVKTLE ERGIGRPSTY 490 500 510 520 530 540 APTLDTIQRR GYVALDNKRF VPTELGQIVL DLIMEFFPEI INVEFTAKME RDLDHVEEGN 550 560 570 580 590 600 TEWVKIIDNF YTDFEKRVKK AESEMKEVEI EPEYAGEDCE LCSSPMVYKM GRYGKFLACS 610 620 630 640 650 660 NFPDCRNTKP IVKQIGVKCP SCGEGNIVER KSKKKRVFYG CDRYPDCEFV SWDKPIERKC 670 680 690 PKCGKMLVEK KLKKGIQVQC VECDYKEEPQ K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of the TopI gene, the gene encoding B. subtilis DNA topoisomerase I." de Jong S. Submitted (AUG-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / 8G5.
[2]	"Cloning and sequencing 7.5 Kbp of DNA from Bacillus subtilis upstream of the codV gene." Foulger D., Errington J. Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L27797 Genomic DNA. Translation: AAA22763.1. AJ000975 Genomic DNA. Translation: CAA04422.1. AL009126 Genomic DNA. Translation: CAB13485.1.
PIR	G69724.
RefSeq	NP_389494.1.
3D structure databases
HSSP	HSSP built from PDB template 1ECL based on UniProtKB P06612.
ModBase	Search...
Genome annotation databases
GeneID	937982.
GenomeReviews	Gene locus BSU16120 in contig AL009126_GR.
KEGG	bsu:BSU16120.
NMPDR	fig|224308.1.peg.1614.
Organism-specific databases
SubtiList	BG11007. topA. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P39814.
OMA	RKFYGCS.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU1613-MON.
BRENDA	5.99.1.2. 150.
Family and domain databases
InterPro	IPR003601. Topo_IA_2. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013826. Topo_IA_cen_sub3. IPR000380. Topo_IA_core. IPR003602. Topo_IA_DNA_bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac. IPR006154. Toprim_dom_subgr. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:1.10.290.10. Topo_IA_cen_sub3. 1 hit.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 3 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
TIGRFAMs	TIGR01051. topA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TOP1_BACSU

Accession

Primary (citable) accession number: P39814

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 3, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)