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P39814 (TOP1_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 1

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene names
Name:topA
Synonyms:topI
Ordered Locus Names:BSU16120
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length691 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. HAMAP-Rule MF_00952

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. HAMAP-Rule MF_00952

Cofactor

Magnesium. Binds two Mg2+ per subunit By similarity. HAMAP-Rule MF_00952

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00952

Sequence similarities

Belongs to the type IA topoisomerase family.

Contains 1 Toprim domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 691691DNA topoisomerase 1 HAMAP-Rule MF_00952
PRO_0000145142

Regions

Domain3 – 114112Toprim
Zinc finger579 – 60527C4-type 1 HAMAP-Rule MF_00952
Zinc finger619 – 64729C4-type 2 HAMAP-Rule MF_00952
Zinc finger660 – 68324C4-type 3 HAMAP-Rule MF_00952
Region163 – 1686Interaction with DNA By similarity

Sites

Active site2981O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Metal binding91Magnesium 1; catalytic By similarity
Metal binding821Magnesium 1; catalytic By similarity
Metal binding821Magnesium 2 By similarity
Metal binding841Magnesium 2 By similarity
Site331Interaction with DNA By similarity
Site1391Interaction with DNA By similarity
Site1401Interaction with DNA By similarity
Site1431Interaction with DNA By similarity
Site1481Interaction with DNA By similarity
Site1551Interaction with DNA By similarity
Site3001Interaction with DNA By similarity
Site4901Interaction with DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
P39814 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 58A45C84E52711BB

FASTA69179,078
        10         20         30         40         50         60 
MSDYLVIVES PAKAKTIERY LGKKYKVKAS MGHVRDLPKS QMGVDIEQNF EPKYITIRGK 

        70         80         90        100        110        120 
GPVLKELKTA AKKAKKVYLA ADPDREGEAI AWHLAHSLDL DLNSDCRVVF NEITKDAIKE 

       130        140        150        160        170        180 
SFKHPRMINM DLVDAQQARR ILDRLVGYKI SPILWKKVKK GLSAGRVQSV ALRLIIDREK 

       190        200        210        220        230        240 
EINDFKPEEY WTIDGTFLKG QETFEASFFG KNGKKLPLNS EADVKEILSQ LKGNQYTVEK 

       250        260        270        280        290        300 
VTKKERKRNP ALPFTTSTLQ QEAARKLNFR AKKTMMIAQQ LYEGIDLGRE GTVGLITYMR 

       310        320        330        340        350        360 
TDSTRISNTA VDEAAAFIDQ TYGKEFLGGK RKPAKKNENA QDAHEAIRPT SVLRKPSELK 

       370        380        390        400        410        420 
AVLGRDQMRL YKLIWERFVA SQMAPAVLDT MSVDLTNNGL TFRANGSKVK FSGFMKVYVE 

       430        440        450        460        470        480 
GKDDQMEEKD RMLPDLQEGD TVLSKDIEPE QHFTQPPPRY TEARLVKTLE ERGIGRPSTY 

       490        500        510        520        530        540 
APTLDTIQRR GYVALDNKRF VPTELGQIVL DLIMEFFPEI INVEFTAKME RDLDHVEEGN 

       550        560        570        580        590        600 
TEWVKIIDNF YTDFEKRVKK AESEMKEVEI EPEYAGEDCE LCSSPMVYKM GRYGKFLACS 

       610        620        630        640        650        660 
NFPDCRNTKP IVKQIGVKCP SCGEGNIVER KSKKKRVFYG CDRYPDCEFV SWDKPIERKC 

       670        680        690 
PKCGKMLVEK KLKKGIQVQC VECDYKEEPQ K 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and sequencing of the TopI gene, the gene encoding B. subtilis DNA topoisomerase I."
de Jong S.
Submitted (AUG-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168 / 8G5.
[2]"Cloning and sequencing 7.5 Kbp of DNA from Bacillus subtilis upstream of the codV gene."
Foulger D., Errington J.
Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[3]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L27797 Genomic DNA. Translation: AAA22763.1.
AJ000975 Genomic DNA. Translation: CAA04422.1.
AL009126 Genomic DNA. Translation: CAB13485.1.
PIRG69724.
RefSeqNP_389494.1. NC_000964.3.

3D structure databases

ProteinModelPortalP39814.
SMRP39814. Positions 2-617.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU16120.

Proteomic databases

PaxDbP39814.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB13485; CAB13485; BSU16120.
GeneID937982.
KEGGbsu:BSU16120.
PATRIC18975029. VBIBacSub10457_1706.

Organism-specific databases

GenoListBSU16120. [Micado]

Phylogenomic databases

eggNOGCOG0551.
HOGENOMHOG000004018.
KOK03168.
OMAMVYKMGR.
OrthoDBEOG6S7XQ9.
ProtClustDBPRK05582.

Enzyme and pathway databases

BioCycBSUB:BSU16120-MONOMER.

Family and domain databases

Gene3D1.10.460.10. 3 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPMF_00952. Topoisom_1_prok.
InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERPTHR11390. PTHR11390. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. SSF56712. 2 hits.
TIGRFAMsTIGR01051. topA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP1_BACSU
AccessionPrimary (citable) accession number: P39814
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: February 19, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList