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P39814

- TOP1_BACSU

UniProt

P39814 - TOP1_BACSU

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Protein
DNA topoisomerase 1
Gene
topA, topI, BSU16120
Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Magnesium. Binds two Mg2+ per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi9 – 91Magnesium 1; catalytic By similarity
Sitei33 – 331Interaction with DNA By similarity
Metal bindingi82 – 821Magnesium 1; catalytic By similarity
Metal bindingi82 – 821Magnesium 2 By similarity
Metal bindingi84 – 841Magnesium 2 By similarity
Sitei139 – 1391Interaction with DNA By similarity
Sitei140 – 1401Interaction with DNA By similarity
Sitei143 – 1431Interaction with DNA By similarity
Sitei148 – 1481Interaction with DNA By similarity
Sitei155 – 1551Interaction with DNA By similarity
Active sitei298 – 2981O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Sitei300 – 3001Interaction with DNA By similarity
Sitei490 – 4901Interaction with DNA By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri579 – 60527C4-type 1UniRule annotation
Add
BLAST
Zinc fingeri619 – 64729C4-type 2UniRule annotation
Add
BLAST
Zinc fingeri660 – 68324C4-type 3UniRule annotation
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciBSUB:BSU16120-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topA
Synonyms:topI
Ordered Locus Names:BSU16120
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU16120. [Micado]

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 691691DNA topoisomerase 1UniRule annotation
PRO_0000145142Add
BLAST

Proteomic databases

PaxDbiP39814.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi224308.BSU16120.

Structurei

3D structure databases

ProteinModelPortaliP39814.
SMRiP39814. Positions 2-617.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 114112Toprim
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni163 – 1686Interaction with DNA By similarity

Sequence similaritiesi

Contains 1 Toprim domain.

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0551.
HOGENOMiHOG000004018.
KOiK03168.
OMAiVVECDKC.
OrthoDBiEOG6S7XQ9.
PhylomeDBiP39814.

Family and domain databases

Gene3Di1.10.460.10. 3 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 2 hits.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39814-1 [UniParc]FASTAAdd to Basket

« Hide

MSDYLVIVES PAKAKTIERY LGKKYKVKAS MGHVRDLPKS QMGVDIEQNF    50
EPKYITIRGK GPVLKELKTA AKKAKKVYLA ADPDREGEAI AWHLAHSLDL 100
DLNSDCRVVF NEITKDAIKE SFKHPRMINM DLVDAQQARR ILDRLVGYKI 150
SPILWKKVKK GLSAGRVQSV ALRLIIDREK EINDFKPEEY WTIDGTFLKG 200
QETFEASFFG KNGKKLPLNS EADVKEILSQ LKGNQYTVEK VTKKERKRNP 250
ALPFTTSTLQ QEAARKLNFR AKKTMMIAQQ LYEGIDLGRE GTVGLITYMR 300
TDSTRISNTA VDEAAAFIDQ TYGKEFLGGK RKPAKKNENA QDAHEAIRPT 350
SVLRKPSELK AVLGRDQMRL YKLIWERFVA SQMAPAVLDT MSVDLTNNGL 400
TFRANGSKVK FSGFMKVYVE GKDDQMEEKD RMLPDLQEGD TVLSKDIEPE 450
QHFTQPPPRY TEARLVKTLE ERGIGRPSTY APTLDTIQRR GYVALDNKRF 500
VPTELGQIVL DLIMEFFPEI INVEFTAKME RDLDHVEEGN TEWVKIIDNF 550
YTDFEKRVKK AESEMKEVEI EPEYAGEDCE LCSSPMVYKM GRYGKFLACS 600
NFPDCRNTKP IVKQIGVKCP SCGEGNIVER KSKKKRVFYG CDRYPDCEFV 650
SWDKPIERKC PKCGKMLVEK KLKKGIQVQC VECDYKEEPQ K 691
Length:691
Mass (Da):79,078
Last modified:February 1, 1995 - v1
Checksum:i58A45C84E52711BB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L27797 Genomic DNA. Translation: AAA22763.1.
AJ000975 Genomic DNA. Translation: CAA04422.1.
AL009126 Genomic DNA. Translation: CAB13485.1.
PIRiG69724.
RefSeqiNP_389494.1. NC_000964.3.
WP_003245599.1. NZ_CM000487.1.

Genome annotation databases

EnsemblBacteriaiCAB13485; CAB13485; BSU16120.
GeneIDi937982.
KEGGibsu:BSU16120.
PATRICi18975029. VBIBacSub10457_1706.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L27797 Genomic DNA. Translation: AAA22763.1 .
AJ000975 Genomic DNA. Translation: CAA04422.1 .
AL009126 Genomic DNA. Translation: CAB13485.1 .
PIRi G69724.
RefSeqi NP_389494.1. NC_000964.3.
WP_003245599.1. NZ_CM000487.1.

3D structure databases

ProteinModelPortali P39814.
SMRi P39814. Positions 2-617.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224308.BSU16120.

Proteomic databases

PaxDbi P39814.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB13485 ; CAB13485 ; BSU16120 .
GeneIDi 937982.
KEGGi bsu:BSU16120.
PATRICi 18975029. VBIBacSub10457_1706.

Organism-specific databases

GenoListi BSU16120. [Micado ]

Phylogenomic databases

eggNOGi COG0551.
HOGENOMi HOG000004018.
KOi K03168.
OMAi VVECDKC.
OrthoDBi EOG6S7XQ9.
PhylomeDBi P39814.

Enzyme and pathway databases

BioCyci BSUB:BSU16120-MONOMER.

Family and domain databases

Gene3Di 1.10.460.10. 3 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPi MF_00952. Topoisom_1_prok.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 2 hits.
TIGRFAMsi TIGR01051. topA_bact. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing of the TopI gene, the gene encoding B. subtilis DNA topoisomerase I."
    de Jong S.
    Submitted (AUG-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168 / 8G5.
  2. "Cloning and sequencing 7.5 Kbp of DNA from Bacillus subtilis upstream of the codV gene."
    Foulger D., Errington J.
    Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.

Entry informationi

Entry nameiTOP1_BACSU
AccessioniPrimary (citable) accession number: P39814
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 3, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi