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Protein

Glutamate synthase [NADPH] large chain

Gene

gltA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH.

Cofactori

Protein has several cofactor binding sites:

Pathwayi: L-glutamate biosynthesis via GLT pathway

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate from 2-oxoglutarate and L-glutamine (NADP(+) route).
Proteins known to be involved in this subpathway in this organism are:
  1. Glutamate synthase [NADPH] small chain (gltB), Glutamate synthase [NADPH] large chain (gltA)
This subpathway is part of the pathway L-glutamate biosynthesis via GLT pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate from 2-oxoglutarate and L-glutamine (NADP(+) route), the pathway L-glutamate biosynthesis via GLT pathway and in Amino-acid biosynthesis.

Pathwayi: nitrogen metabolism

This protein is involved in the pathway nitrogen metabolism, which is part of Energy metabolism.
View all proteins of this organism that are known to be involved in the pathway nitrogen metabolism and in Energy metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei22For GATase activityBy similarity1
Metal bindingi1113Iron-sulfur (3Fe-4S)By similarity1
Metal bindingi1119Iron-sulfur (3Fe-4S)By similarity1
Metal bindingi1124Iron-sulfur (3Fe-4S)By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi1060 – 1112FMNBy similarityAdd BLAST53

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Glutamate biosynthesis
Ligand3Fe-4S, FAD, Flavoprotein, FMN, Iron, Iron-sulfur, Metal-binding, NADP

Enzyme and pathway databases

BioCyciBSUB:BSU18450-MONOMER
BRENDAi1.4.1.13 658
SABIO-RKiP39812
UniPathwayiUPA00045
UPA00634; UER00689

Protein family/group databases

MEROPSiC44.003

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate synthase [NADPH] large chain (EC:1.4.1.13)
Alternative name(s):
NADPH-GOGAT
Gene namesi
Name:gltA
Ordered Locus Names:BSU18450
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001707971 – 1520Glutamate synthase [NADPH] large chainAdd BLAST1520

Proteomic databases

PaxDbiP39812
PRIDEiP39812

Expressioni

Inductioni

The gltAB operon is positively regulated by GltC and negatively regulated by TnrA under nitrogen-limited conditions.

Interactioni

Subunit structurei

Aggregate of 4 catalytic active heterodimers, consisting of a large and a small subunit.By similarity

Protein-protein interaction databases

IntActiP39812, 1 interactor
MINTiP39812
STRINGi224308.Bsubs1_010100010166

Structurei

3D structure databases

ProteinModelPortaliP39812
SMRiP39812
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 415Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST394

Sequence similaritiesi

Belongs to the glutamate synthase family.Curated

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4105CBC Bacteria
COG0067 LUCA
COG0069 LUCA
COG0070 LUCA
HOGENOMiHOG000031558
InParanoidiP39812
KOiK00265
OMAiRFKTGAM
PhylomeDBiP39812

Family and domain databases

CDDicd00982 gltB_C, 1 hit
cd02808 GltS_FMN, 1 hit
Gene3Di2.160.20.60, 1 hit
3.20.20.70, 5 hits
3.60.20.10, 2 hits
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR017932 GATase_2_dom
IPR002489 Glu_synth_asu_C
IPR036485 Glu_synth_asu_C_sf
IPR006982 Glu_synth_centr_N
IPR002932 Glu_synthdom
IPR029055 Ntn_hydrolases_N
PfamiView protein in Pfam
PF00310 GATase_2, 1 hit
PF04898 Glu_syn_central, 1 hit
PF01645 Glu_synthase, 1 hit
PF01493 GXGXG, 1 hit
SUPFAMiSSF56235 SSF56235, 1 hit
SSF69336 SSF69336, 1 hit
PROSITEiView protein in PROSITE
PS51278 GATASE_TYPE_2, 1 hit

Sequencei

Sequence statusi: Complete.

P39812-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTYNQMPKAQ GLYRPEFEHD ACGIGLYAHL KGKQTHDIVK QGLKMLCQLD
60 70 80 90 100
HRGGQGSDPD TGDGAGLLVQ IPDAFFRKEC KNINLPEKER YGVGMVFFSQ
110 120 130 140 150
KEDERKKIEK QINALIEQEG QVVLGWRTVP VNVGKIGTVA QKSCPFVRQV
160 170 180 190 200
FIGASSDLKD NLSFERKLYV IRKQAENWGV TEGLDFYFAS LSSQTIVYKG
210 220 230 240 250
LLTPEQVDAF YSDLQDEAFV SAFALVHSRF STNTFPTWER AHPNRYLVHN
260 270 280 290 300
GEINTLRGNI NWMRAREQQF VSESFGEDLN KILPILNADG SDSSILDNAF
310 320 330 340 350
EFFVMAGRKP AHTAMMLIPE PWTENTHMSK EKRAFYEYHS SLMEPWDGPT
360 370 380 390 400
AISFTDGKQI GAILDRNGLR PARYYVTKDD YIIFSSEVGV IEVEQENVLY
410 420 430 440 450
KNRLEPGKML LIDLEEGRII SDEEVKTQIA TEYPYQKWLE EELVQVNPDP
460 470 480 490 500
ESREEEQFSD LLTRQKAFGY TYEDIQKYLI PVIKEGKDPL GSMGNDAPLA
510 520 530 540 550
VLSDRAQSLF NYFKQLFAQV TNPPIDAIRE QLVTSTMTWL GAEGDLLHPS
560 570 580 590 600
ERNVRRIKLY TPVLSNEQFY ALKTIVHPDL KSQKIDVLFS EDLERGLKDM
610 620 630 640 650
FTQAEKAISQ GVSLLILSDK KMNERLTPIP PLLAVSALHQ HLIRKGLRTK
660 670 680 690 700
VSIIVESGEA REVHHFAALI GYGADAINPY LAYATYKQEI DEGRLDISYE
710 720 730 740 750
EAVSKYGKSI TEGVVKVMSK MGISTVQSYR GAQIFEAVGI SRDVIDRYFS
760 770 780 790 800
GTASQLGGID LQTIAEEAQR RHREAYQDDY SKTLEPGSDF QWRNGGEHHA
810 820 830 840 850
FNPKTIHTLQ WACRRNDYNL FKQYTKAADE ERIGFLRNLF AFDGNRKPLK
860 870 880 890 900
LEEVESAESI VKRFKTGAMS FGSLSKEAHE ALAIAMNRLG GKSNSGEGGE
910 920 930 940 950
DPKRFVPDEN GDDRRSAIKQ IASGRFGVKS HYLVNADELQ IKMAQGAKPG
960 970 980 990 1000
EGGQLPGNKV YPWVADVRGS TPGVGLISPP PHHDIYSIED LAQLIHDLKN
1010 1020 1030 1040 1050
ANRDARISVK LVSKAGVGTI AAGVAKATAD VIVISGYDGG TGASPKTSIK
1060 1070 1080 1090 1100
HTGLPWELGL AEAHQTLMLN GLRDRVVLET DGKLMTGRDV VMAALLGAEE
1110 1120 1130 1140 1150
FGFATAPLVV LGCVMMRACH LDTCPVGVAT QNPELRKKFM GDPDHIVNYM
1160 1170 1180 1190 1200
LFIAEEVREY MAALGFKTFD EMIGRTDVLH ASERAKEHWK ASQLDLSTLL
1210 1220 1230 1240 1250
YQPEGVRTFQ SPQNHKIDQS LDITTILPAV QEAIESGKEA DISIEINNTN
1260 1270 1280 1290 1300
RVAGTITGSE ISKRYGEEGL PEDTIKLHFT GSAGQSFGAF VPKGMTLYLD
1310 1320 1330 1340 1350
GDSNDYVGKG LSGGKIIVKS SEGFNSASDD NVIIGNVAFY GATSGEAYIN
1360 1370 1380 1390 1400
GRAGERFAVR NSGVNVVVEG IGDHGCEYMT GGSVVVLGDV GKNFAAGMSG
1410 1420 1430 1440 1450
GIAYVLTEDV KAFKRKCNLE MILFESLEDE KEIQQIKAML ERHTAYTNSQ
1460 1470 1480 1490 1500
KAEDLLDQWE DSVKKFVKVI PKNYKQMLAS IEEQKAAGLS DEEAIMFAFE
1510 1520
ANTKPKQNTA ASGQKQAVVQ
Length:1,520
Mass (Da):168,772
Last modified:June 16, 2009 - v3
Checksum:i156A22E65A8CC423
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA Translation: CAB13728.2
M28509 Unassigned DNA Translation: AAA16438.1
PIRiG69634
RefSeqiNP_389727.2, NC_000964.3
WP_009967365.1, NZ_CP010052.1

Genome annotation databases

EnsemblBacteriaiCAB13728; CAB13728; BSU18450
GeneIDi940024
KEGGibsu:BSU18450
PATRICifig|224308.179.peg.2012

Similar proteinsi

Entry informationi

Entry nameiGLTA_BACSU
AccessioniPrimary (citable) accession number: P39812
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: June 16, 2009
Last modified: May 23, 2018
This is version 137 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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