P39804 (IPI_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Intracellular proteinase inhibitor

Alternative name(s):
BsuPI

Gene names

Name:	ipi
Ordered Locus Names:	BSU11130

Organism

Bacillus subtilis (strain 168) [Reference proteome] [HAMAP]

Taxonomic identifier

224308 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus subtilis group › Bacillus subtilis › Bacillus subtilis subsp. subtilis

Protein attributes

Sequence length	119 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Directly regulates the major intracellular proteinase (ISP-1) activity in vivo. Inhibits ISP-1 in the early stages of sporulation. It may be then inactivated by a membrane-bound proteinase.
Subcellular location	Cytoplasm.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Protease inhibitor Serine protease inhibitor
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	serine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 119

119

Intracellular proteinase inhibitor

PRO_0000084223

Secondary structure

1

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119

Helix Strand Turn

Details...

Beta strand

5 – 14

10

Beta strand

19 – 27

9

Beta strand

29 – 31

3

Beta strand

33 – 40

8

Beta strand

43 – 48

6

Beta strand

54 – 57

4

Turn

58 – 61

4

Beta strand

69 – 73

5

Beta strand

78 – 87

10

Beta strand

91 – 100

10

Beta strand

111 – 118

8

Sequences

	Sequence		Length	Mass (Da)
	P39804 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: DDACBCA16C721308	FASTA	119	14,074
	10 20 30 40 50 60 MENQEVVLSI DAIQEPEQIK FNMSLKNQSE RAIEFQFSTG QKFELVVYDS EHKERYRYSK 70 80 90 100 110 EKMFTQAFQN LTLESGETYD FSDVWKEVPE PGTYEVKVTF KGRAENLKQV QAVQQFEVK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the gene encoding an intracellular proteinase inhibitor of Bacillus subtilis and its role in regulation of the major intracellular proteinase." Shiga Y., Yamagata H., Udaka S. J. Bacteriol. 175:7130-7137(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10. Strain: 168 / DB104.
[2]	"A 10.3 kbp segment from nprB to argJ at the 102 degrees region of the Bacillus subtilis chromosome." Levine A., Vannier F., Roche B., Autret S., Mavel D., Seror S.J. Microbiology 143:175-177(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"Sequencing of regions downstream of addA (98 degrees) and citG (289 degrees) in Bacillus subtilis." Medina N., Vannier F., Roche B., Autret S., Levine A., Seror S.J. Microbiology 143:3305-3308(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[4]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D16311 Genomic DNA. Translation: BAA03820.1.
Z79580 Genomic DNA. Translation: CAB01835.1.
Y09476 Genomic DNA. Translation: CAA70631.1.
AL009126 Genomic DNA. Translation: CAB12953.1.

PIR

C49921.

RefSeq

NP_388994.1. NC_000964.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3ISY	X-ray	2.61	A	1-119	[»]

ProteinModelPortal

P39804.

SMR

P39804. Positions 3-119.

ModBase

Search...

Protein-protein interaction databases

IntAct

P39804. 1 interaction.

STRING

224308.BSU11130.

Proteomic databases

PaxDb

P39804.

Protocols and materials databases

DNASU

936380.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

CAB12953; CAB12953; BSU11130.

GeneID

936380.

KEGG

bsu:BSU11130.

PATRIC

18973932. VBIBacSub10457_1160.

Organism-specific databases

GenoList

BSU11130. [Micado]

Phylogenomic databases

eggNOG

NOG70929.

HOGENOM

HOG000008848.

OMA

MFTQAFQ.

ProtClustDB

CLSK887048.

Enzyme and pathway databases

BioCyc

BSUB:BSU11130-MONOMER.

Family and domain databases

InterPro

IPR020481. Prot_inh_BsuPI.
[Graphical view]

Pfam

PF12690. BsuPI. 1 hit.
[Graphical view]

ProDom

PD076312. Intracellular_proteinase_inh. 1 hit.
[Graphical view] [Entries sharing at least one domain]

ProtoNet

Search...

Other

EvolutionaryTrace

P39804.

Entry information

Entry name

IPI_BACSU

Accession

Primary (citable) accession number: P39804

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program