Reviewed, UniProtKB/Swiss-Prot P39800 (XLYA_BACSU)

Names and origin

Protein names

Recommended name:
    N-acetylmuramoyl-L-alanine amidase xlyA
    EC=3.5.1.28
Alternative name(s):
    Cell wall hydrolase
    Autolysin

Gene names

Name:	xlyA
Ordered Locus Names:	BSU12810

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation.
Catalytic activity	Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family. Contains 1 LysM repeat.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation Competence Sporulation
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW establishment of competence for transformation Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan catabolic process Inferred from electronic annotation. Source: InterPro sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N-acetylmuramoyl-L-alanine amidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 44	44	Potential
	Chain	45 – 297	253	N-acetylmuramoyl-L-alanine amidase xlyA		PRO_0000006457
Regions
	Repeat	161 – 204	44	LysM

Sequences

	Sequence		Length	Mass (Da)
	P39800-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 33DB710059A3EC72	FASTA	297	31,913
	10 20 30 40 50 60 MVNIIQDFIP VGANNRPGYA MTPLYITVHN TANTAVGADA AAHARYLKNP DTTTSWHFTV 70 80 90 100 110 120 DDTEIYQHLP LNENGWHAGD GNGSGNRASI GIEICENADG DFAKATANAQ WLIKTLMAEH 130 140 150 160 170 180 NISLANVVPH KYWSGKECPR KLLDTWDSFK AGIGGGGSQT YVVKQGDTLT SIARAFGVTV 190 200 210 220 230 240 AQLQEWNNIE DPNLIRVGQV LIVSAPSAAE KPELYPLPDG IIQLTTPYTS GEHVFQVQRA 250 260 270 280 290 LAALYFYPDK GAVNNGIDGV YGPKTADAVA RFQSVNGLTA DGIYGPATKE KIAAQLS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Krogh S., Joergensen S.T., Diderichsen B., Devine K.M. Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / SO113.
[2]	"Lytic enzymes associated with defective prophages of Bacillus subtilis: sequencing and characterization of the region comprising the N-acetylmuramoyl-L-alanine amidase gene of prophage PBSX." Longchamp P.F., Mauel C., Karamata D. Microbiology 140:1855-1867(1994) [PubMed: 7921239] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"Lysis genes of the Bacillus subtilis defective prophage PBSX." Krogh S., Jorgensen S.T., Devine K.M. J. Bacteriol. 180:2110-2117(1998) [PubMed: 9555893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[4]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[5]	"Sequence of the Bacillus subtilis genome between xlyA and ykoR." Devine K.M. Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 195-297. Strain: 168.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z36941 Genomic DNA. Translation: CAA85403.1. L25924 Genomic DNA. Translation: AAA22645.1. Z70177 Genomic DNA. Translation: CAA94049.1. AL009126 Genomic DNA. Translation: CAB13138.1. AJ002571 Genomic DNA. Translation: CAA05561.1.
PIR	I39938.
RefSeq	NP_389164.1.
3D structure databases
HSSP	HSSP built from PDB template 1LBU based on UniProtKB P00733.
ModBase	Search...
Genome annotation databases
GeneID	939869.
GenomeReviews	Gene locus BSU12810 in contig AL009126_GR.
KEGG	bsu:BSU12810.
NMPDR	fig|224308.1.peg.1283.
Organism-specific databases
SubtiList	BG10962. xlyA. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P39800.
OMA	WHAGDGN.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU1283-MON.
BRENDA	3.5.1.28. 150.
Family and domain databases
InterPro	IPR002502. Amidase_2. IPR002477. Peptidoglycan-bd-like. IPR018392. Peptidoglycan-bd_lysin. IPR002482. Peptidoglycan-bd_Lysin_sg. [Graphical view]
Gene3D	G3DSA:3.40.80.10. Amidase_2. 1 hit. G3DSA:1.10.101.10. PGBD_1. 1 hit.
Pfam	PF01510. Amidase_2. 1 hit. PF01476. LysM. 1 hit. PF01471. PG_binding_1. 1 hit. [Graphical view]
SMART	SM00644. Ami_2. 1 hit. SM00257. LysM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XLYA_BACSU

Accession

Primary (citable) accession number: P39800

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 3, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)