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Protein

Endonuclease III

Gene

nth

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi189Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi196Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi199Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi205Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Glycosidase, Hydrolase, Lyase
Biological processDNA damage, DNA repair
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciBSUB:BSU22340-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease IIIUniRule annotation (EC:4.2.99.18UniRule annotation)
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation
Gene namesi
Name:nthUniRule annotation
Synonyms:jooB, ypoB
Ordered Locus Names:BSU22340
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene have a 5-fold increased spontaneous mutation frequency. A triple MutS/MutL/nth disruption has a 280-fold increased spontaneous mutation frequency.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001022131 – 219Endonuclease IIIAdd BLAST219

Proteomic databases

PaxDbiP39788
PRIDEiP39788

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100012286

Structurei

3D structure databases

ProteinModelPortaliP39788
SMRiP39788
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini109 – 128HhHUniRule annotationAdd BLAST20

Sequence similaritiesi

Belongs to the Nth/MutY family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CSM Bacteria
COG0177 LUCA
HOGENOMiHOG000252208
InParanoidiP39788
KOiK10773
OMAiKAKNPLC
PhylomeDBiP39788

Family and domain databases

CDDicd00056 ENDO3c, 1 hit
Gene3Di1.10.1670.10, 2 hits
HAMAPiMF_00942 Nth, 1 hit
InterProiView protein in InterPro
IPR011257 DNA_glycosylase
IPR004036 Endonuclease-III-like_CS2
IPR003651 Endonuclease3_FeS-loop_motif
IPR004035 Endouclease-III_FeS-bd_BS
IPR003265 HhH-GPD_domain
IPR000445 HhH_motif
IPR023170 HTH_base_excis_C
IPR005759 Nth
PfamiView protein in Pfam
PF10576 EndIII_4Fe-2S, 1 hit
PF00633 HHH, 1 hit
PF00730 HhH-GPD, 1 hit
SMARTiView protein in SMART
SM00478 ENDO3c, 1 hit
SM00525 FES, 1 hit
SUPFAMiSSF48150 SSF48150, 1 hit
TIGRFAMsiTIGR01083 nth, 1 hit
PROSITEiView protein in PROSITE
PS00764 ENDONUCLEASE_III_1, 1 hit
PS01155 ENDONUCLEASE_III_2, 1 hit

Sequencei

Sequence statusi: Complete.

P39788-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNLKQIEFC LDKIGDMFPH AECELVHSNP FELVVAVALS AQCTDALVNR
60 70 80 90 100
VTKTLFQKYK RPEDYLAVPL EELQQDIKSI GLYRNKAKNI QKLSKMIIED
110 120 130 140 150
YGGEVPRDRD ELVKLPGVGR KTANVVVSVA FGVPAIAVDT HVERVSKRLG
160 170 180 190 200
ICRWKDSVLE VEKTLMRKVP KEDWSVTHHR LIFFGRYHCK AQSPRCAECP
210
LLSLCREGQK RDKKGLVKR
Length:219
Mass (Da):25,000
Last modified:February 1, 1995 - v1
Checksum:iC38C66DA7948BA40
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11289 Genomic DNA Translation: AAA80005.1
L47709 Genomic DNA Translation: AAB38457.1
AL009126 Genomic DNA Translation: CAB14150.1
PIRiI40525
RefSeqiNP_390115.1, NC_000964.3
WP_009967580.1, NZ_JNCM01000036.1

Genome annotation databases

EnsemblBacteriaiCAB14150; CAB14150; BSU22340
GeneIDi939036
KEGGibsu:BSU22340
PATRICifig|224308.179.peg.2438

Similar proteinsi

Entry informationi

Entry nameiEND3_BACSU
AccessioniPrimary (citable) accession number: P39788
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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