P39774 (SPAB_BACIU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Subtilin biosynthesis protein SpaB

Gene names

Name:	spaB
Synonyms:	spaD, spaE

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus subtilis group

Protein attributes

Sequence length	1030 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Involved in the post-translational modification of the lantibiotic subtilin.
Subcellular location	Cell membrane. Note: Possibly associated with, and anchored to, the cytoplasmic side of the membrane.
Sequence similarities	To S.epidermidis EpiB and L.lactis NisB.
Caution	Was originally (Ref.3) proposed to encode for two separate adjacent ORFs, SpaE and SpaD.
Sequence caution	The sequence AAA22769.1 differs from that shown. Reason: Erroneous initiation. The sequence AAA22774.1 differs from that shown. Reason: Frameshift at position 852. The sequence AAA22775.1 differs from that shown. Reason: Frameshift at position 852.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Membrane
Gene Ontology (GO)
Biological_process	transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1030	1030	Subtilin biosynthesis protein SpaB		PRO_0000072091
Experimental info
	Sequence conflict	126	1	L → F in AAA22774. Ref.3
	Sequence conflict	261	1	D → N in AAA22774. Ref.3
	Sequence conflict	304 – 305	2	LA → AG in AAA22774. Ref.3
	Sequence conflict	344	1	H → K in AAA22774. Ref.3
	Sequence conflict	553	1	N → I in AAA22774. Ref.3
	Sequence conflict	584	1	F → L in AAA22774. Ref.3
	Sequence conflict	647	1	P → A in AAA22774. Ref.3
	Sequence conflict	730	1	E → V in AAA22774. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P39774 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 89F3B147E02A9DF8	FASTA	1,030	120,529
	10 20 30 40 50 60 MKSLYTPTDY YMIRVPLVHQ DLKNENSQDI DQLLHDLCND SLFREQILVS SRTLYETIHT 70 80 90 100 110 120 FLQAPDKLKG KKKRNFQQAI LKYATRRATR TTPFGLFSSV GIGSFSDKNH LSFNQHSFYK 130 140 150 160 170 180 KARVDLEWLY QLIRKLENEY TDRLSFTLNS ACYIKGDRAY LLYSTDGKSE EVSVRATSVF 190 200 210 220 230 240 YLINELCGES AAYQDIIRCL IDNYPNTPIN KINQYVADLI DKEFLISNLR PPMTVSDQFQ 250 260 270 280 290 300 YLIDQAESRH IPNELIQACK DIQYQIDAYN RITIGEGEHQ YLNLIETMNK LIKASSPLQV 310 320 330 340 350 360 DAGLADSSIQ LDNETSLAIS ELASMFTYMA SPSANTLDHL EKYHNVFLER YGYEREVPLL 370 380 390 400 410 420 EMLCSSTGIG APATYTNPAN EFFEETSFGE QFSPEMKQFF MRKYFESVRK KAPIQLDDET 430 440 450 460 470 480 FHRICNSEIA DEEIPLSFEL NFFVKLRNGR VKLYLGPNVG STRAGKTFGR FSHMSDSISE 490 500 510 520 530 540 IIKTLHNKEK ELTECNTKVC ELSIVPNQTR SGNVTRNVSY REKEMSLFTN SALHLNDSVK 550 560 570 580 590 600 AEDILIGINK DHNFYARHKT TGEILSFESN HMFNPLLMTN AVRFLLEISR DGKRKWNDFP 610 620 630 640 650 660 WFSIYSDFKY IPEIKYKEIT LSCEQWLIYK NDLSMHSNAS LEEIKSPFFE FHRTYELPQT 670 680 690 700 710 720 FYIVNADNRL LIDIENDCTL DVFFWELKKT NHNQPLQLVA VEHDADALMD RNQNDYSGEI 730 740 750 760 770 780 VVPLLRKQPE KPLYLPVLNA IEGSGSDRIK MPFEDWLFIK LYCKQTREEE LIAFEIADFY 790 800 810 820 830 840 NQISDQYPVR HFFMRYRDPK PHIRLRFNGK AEVLYSLFPQ LLNWLKSLRE KGLVSESVIT 850 860 870 880 890 900 QYEREIERYG GLSLMEAAEQ LFCEDSKVVE MMIRMHRMKD ITISKEIAGM VSVIQFLEQF 910 920 930 940 950 960 ELTFEEQLTF LERNSLQNEY RTEFKKDREM YIEICNSDRD WDNLKKTSDG GMLYETLKTR 970 980 990 1000 1010 1020 KMAAAHYAFL IKKAFDNKDE VYSRIGSIIH LHCNRLFGTD RELENKILTL CRHSLYAQRY 1030 QKMNGSLAWK

References

[1]	"Growth phase-dependent regulation and membrane localization of SpaB, a protein involved in biosynthesis of the lantibiotic subtilin." Gutowski-Eckel Z., Klein C., Siegers K., Bohm K., Hammelmann M., Entian K.-D. Appl. Environ. Microbiol. 60:1-11(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 6633 / PCI 219 / NRS 231.
[2]	"Genes involved in self-protection against the lantibiotic subtilin produced by Bacillus subtilis ATCC 6633." Klein C., Entian K.-D. Appl. Environ. Microbiol. 60:2793-2801(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 6633 / PCI 219 / NRS 231.
[3]	"Determination of the sequence of spaE and identification of a promoter in the subtilin (spa) operon in Bacillus subtilis." Chung Y.J., Hansen J.N. J. Bacteriol. 174:6699-6702(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 6633 / PCI 219 / NRS 231.
[4]	"Analysis of genes involved in biosynthesis of the lantibiotic subtilin." Klein C., Kaletta C., Schnell N., Entian K.-D. Appl. Environ. Microbiol. 58:132-142(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 641-1030. Strain: ATCC 6633 / PCI 219 / NRS 231.
[5]	"The subtilin gene of Bacillus subtilis ATCC 6633 is encoded in an operon that contains a homolog of the hemolysin B transport protein." Chung Y.J., Steen M.T., Hansen J.N. J. Bacteriol. 174:1417-1422(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 852-1030. Strain: ATCC 6633 / PCI 219 / NRS 231.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L24075 Genomic DNA. Translation: AAA22779.1. U09819 Genomic DNA. Translation: AAB91586.1. M99263 Genomic DNA. Translation: AAA22774.1. Frameshift. M99263 Genomic DNA. Translation: AAA22775.1. Frameshift. M86869 Genomic DNA. Translation: AAA22837.1. M83944 Genomic DNA. Translation: AAA22769.1. Different initiation. U38418 Genomic DNA. Translation: AAB01536.1.
PIR	I39987.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR006827. Lant_deHydtase_C. IPR006826. Lant_deHydtase_N. IPR023809. Thiopep_bacteriocin_synth_dom. [Graphical view]
Pfam	PF04738. Lant_dehyd_C. 1 hit. PF04737. Lant_dehyd_N. 1 hit. PF14028. SpaB_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR03891. thiopep_ocin. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SPAB_BACIU

Accession

Primary (citable) accession number: P39774
Secondary accession number(s): P33114, P36360

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	November 1, 1997
Last modified:	April 3, 2013
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program