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P39774

- SPAB_BACIU

UniProt

P39774 - SPAB_BACIU

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Protein

Subtilin biosynthesis protein SpaB

Gene

spaB

Organism
Bacillus subtilis
Status
Reviewed - Annotation score: 2 out of 5- Protein predictedi

Functioni

Involved in the post-translational modification of the lantibiotic subtilin.

GO - Biological processi

  1. transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Subtilin biosynthesis protein SpaB
Gene namesi
Name:spaB
Synonyms:spaD, spaE
OrganismiBacillus subtilis
Taxonomic identifieri1423 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

Cell membrane
Note: Possibly associated with, and anchored to, the cytoplasmic side of the membrane.

GO - Cellular componenti

  1. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10301030Subtilin biosynthesis protein SpaBPRO_0000072091Add
BLAST

Interactioni

Protein-protein interaction databases

MINTiMINT-1521998.

Family & Domainsi

Sequence similaritiesi

To S.epidermidis EpiB and L.lactis NisB.Curated

Family and domain databases

InterProiIPR006827. Lant_deHydtase_C.
IPR006826. Lant_deHydtase_N.
IPR023809. Thiopep_bacteriocin_synth_dom.
[Graphical view]
PfamiPF04738. Lant_dehyd_C. 1 hit.
PF04737. Lant_dehyd_N. 1 hit.
PF14028. SpaB_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03891. thiopep_ocin. 1 hit.

Sequencei

Sequence statusi: Complete.

P39774-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MKSLYTPTDY YMIRVPLVHQ DLKNENSQDI DQLLHDLCND SLFREQILVS
60 70 80 90 100
SRTLYETIHT FLQAPDKLKG KKKRNFQQAI LKYATRRATR TTPFGLFSSV
110 120 130 140 150
GIGSFSDKNH LSFNQHSFYK KARVDLEWLY QLIRKLENEY TDRLSFTLNS
160 170 180 190 200
ACYIKGDRAY LLYSTDGKSE EVSVRATSVF YLINELCGES AAYQDIIRCL
210 220 230 240 250
IDNYPNTPIN KINQYVADLI DKEFLISNLR PPMTVSDQFQ YLIDQAESRH
260 270 280 290 300
IPNELIQACK DIQYQIDAYN RITIGEGEHQ YLNLIETMNK LIKASSPLQV
310 320 330 340 350
DAGLADSSIQ LDNETSLAIS ELASMFTYMA SPSANTLDHL EKYHNVFLER
360 370 380 390 400
YGYEREVPLL EMLCSSTGIG APATYTNPAN EFFEETSFGE QFSPEMKQFF
410 420 430 440 450
MRKYFESVRK KAPIQLDDET FHRICNSEIA DEEIPLSFEL NFFVKLRNGR
460 470 480 490 500
VKLYLGPNVG STRAGKTFGR FSHMSDSISE IIKTLHNKEK ELTECNTKVC
510 520 530 540 550
ELSIVPNQTR SGNVTRNVSY REKEMSLFTN SALHLNDSVK AEDILIGINK
560 570 580 590 600
DHNFYARHKT TGEILSFESN HMFNPLLMTN AVRFLLEISR DGKRKWNDFP
610 620 630 640 650
WFSIYSDFKY IPEIKYKEIT LSCEQWLIYK NDLSMHSNAS LEEIKSPFFE
660 670 680 690 700
FHRTYELPQT FYIVNADNRL LIDIENDCTL DVFFWELKKT NHNQPLQLVA
710 720 730 740 750
VEHDADALMD RNQNDYSGEI VVPLLRKQPE KPLYLPVLNA IEGSGSDRIK
760 770 780 790 800
MPFEDWLFIK LYCKQTREEE LIAFEIADFY NQISDQYPVR HFFMRYRDPK
810 820 830 840 850
PHIRLRFNGK AEVLYSLFPQ LLNWLKSLRE KGLVSESVIT QYEREIERYG
860 870 880 890 900
GLSLMEAAEQ LFCEDSKVVE MMIRMHRMKD ITISKEIAGM VSVIQFLEQF
910 920 930 940 950
ELTFEEQLTF LERNSLQNEY RTEFKKDREM YIEICNSDRD WDNLKKTSDG
960 970 980 990 1000
GMLYETLKTR KMAAAHYAFL IKKAFDNKDE VYSRIGSIIH LHCNRLFGTD
1010 1020 1030
RELENKILTL CRHSLYAQRY QKMNGSLAWK
Length:1,030
Mass (Da):120,529
Last modified:November 1, 1997 - v2
Checksum:i89F3B147E02A9DF8
GO

Sequence cautioni

The sequence AAA22769.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAA22774.1 differs from that shown. Reason: Frameshift at position 852. Curated
The sequence AAA22775.1 differs from that shown. Reason: Frameshift at position 852. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti126 – 1261L → F in AAA22774. (PubMed:1400221)Curated
Sequence conflicti261 – 2611D → N in AAA22774. (PubMed:1400221)Curated
Sequence conflicti304 – 3052LA → AG in AAA22774. (PubMed:1400221)Curated
Sequence conflicti344 – 3441H → K in AAA22774. (PubMed:1400221)Curated
Sequence conflicti553 – 5531N → I in AAA22774. (PubMed:1400221)Curated
Sequence conflicti584 – 5841F → L in AAA22774. (PubMed:1400221)Curated
Sequence conflicti647 – 6471P → A in AAA22774. (PubMed:1400221)Curated
Sequence conflicti730 – 7301E → V in AAA22774. (PubMed:1400221)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L24075 Genomic DNA. Translation: AAA22779.1.
U09819 Genomic DNA. Translation: AAB91586.1.
M99263 Genomic DNA. Translation: AAA22774.1. Frameshift.
M99263 Genomic DNA. Translation: AAA22775.1. Frameshift.
M86869 Genomic DNA. Translation: AAA22837.1.
M83944 Genomic DNA. Translation: AAA22769.1. Different initiation.
U38418 Genomic DNA. Translation: AAB01536.1.
PIRiI39987.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L24075 Genomic DNA. Translation: AAA22779.1 .
U09819 Genomic DNA. Translation: AAB91586.1 .
M99263 Genomic DNA. Translation: AAA22774.1 . Frameshift.
M99263 Genomic DNA. Translation: AAA22775.1 . Frameshift.
M86869 Genomic DNA. Translation: AAA22837.1 .
M83944 Genomic DNA. Translation: AAA22769.1 . Different initiation.
U38418 Genomic DNA. Translation: AAB01536.1 .
PIRi I39987.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-1521998.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR006827. Lant_deHydtase_C.
IPR006826. Lant_deHydtase_N.
IPR023809. Thiopep_bacteriocin_synth_dom.
[Graphical view ]
Pfami PF04738. Lant_dehyd_C. 1 hit.
PF04737. Lant_dehyd_N. 1 hit.
PF14028. SpaB_C. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR03891. thiopep_ocin. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Growth phase-dependent regulation and membrane localization of SpaB, a protein involved in biosynthesis of the lantibiotic subtilin."
    Gutowski-Eckel Z., Klein C., Siegers K., Bohm K., Hammelmann M., Entian K.-D.
    Appl. Environ. Microbiol. 60:1-11(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 6633 / PCI 219 / NRS 231.
  2. "Genes involved in self-protection against the lantibiotic subtilin produced by Bacillus subtilis ATCC 6633."
    Klein C., Entian K.-D.
    Appl. Environ. Microbiol. 60:2793-2801(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 6633 / PCI 219 / NRS 231.
  3. "Determination of the sequence of spaE and identification of a promoter in the subtilin (spa) operon in Bacillus subtilis."
    Chung Y.J., Hansen J.N.
    J. Bacteriol. 174:6699-6702(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 6633 / PCI 219 / NRS 231.
  4. "Analysis of genes involved in biosynthesis of the lantibiotic subtilin."
    Klein C., Kaletta C., Schnell N., Entian K.-D.
    Appl. Environ. Microbiol. 58:132-142(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 641-1030.
    Strain: ATCC 6633 / PCI 219 / NRS 231.
  5. "The subtilin gene of Bacillus subtilis ATCC 6633 is encoded in an operon that contains a homolog of the hemolysin B transport protein."
    Chung Y.J., Steen M.T., Hansen J.N.
    J. Bacteriol. 174:1417-1422(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 852-1030.
    Strain: ATCC 6633 / PCI 219 / NRS 231.

Entry informationi

Entry nameiSPAB_BACIU
AccessioniPrimary (citable) accession number: P39774
Secondary accession number(s): P33114, P36360
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 1, 1997
Last modified: October 29, 2014
This is version 56 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally proposed to code for two separate adjacent ORFs, SpaE and SpaD.1 Publication

External Data

Dasty 3