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P39774 (SPAB_BACIU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Subtilin biosynthesis protein SpaB
Gene names
Name:spaB
Synonyms:spaD, spaE
OrganismBacillus subtilis
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length1030 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Involved in the post-translational modification of the lantibiotic subtilin.

Subcellular location

Cell membrane. Note: Possibly associated with, and anchored to, the cytoplasmic side of the membrane.

Sequence similarities

To S.epidermidis EpiB and L.lactis NisB.

Caution

Was originally (Ref.3) proposed to code for two separate adjacent ORFs, SpaE and SpaD.

Sequence caution

The sequence AAA22769.1 differs from that shown. Reason: Erroneous initiation.

The sequence AAA22774.1 differs from that shown. Reason: Frameshift at position 852.

The sequence AAA22775.1 differs from that shown. Reason: Frameshift at position 852.

Ontologies

Keywords
   Biological processTransport
   Cellular componentCell membrane
Membrane
Gene Ontology (GO)
   Biological_processtransport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10301030Subtilin biosynthesis protein SpaB
PRO_0000072091

Experimental info

Sequence conflict1261L → F in AAA22774. Ref.3
Sequence conflict2611D → N in AAA22774. Ref.3
Sequence conflict304 – 3052LA → AG in AAA22774. Ref.3
Sequence conflict3441H → K in AAA22774. Ref.3
Sequence conflict5531N → I in AAA22774. Ref.3
Sequence conflict5841F → L in AAA22774. Ref.3
Sequence conflict6471P → A in AAA22774. Ref.3
Sequence conflict7301E → V in AAA22774. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P39774 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: 89F3B147E02A9DF8

FASTA1,030120,529
        10         20         30         40         50         60 
MKSLYTPTDY YMIRVPLVHQ DLKNENSQDI DQLLHDLCND SLFREQILVS SRTLYETIHT 

        70         80         90        100        110        120 
FLQAPDKLKG KKKRNFQQAI LKYATRRATR TTPFGLFSSV GIGSFSDKNH LSFNQHSFYK 

       130        140        150        160        170        180 
KARVDLEWLY QLIRKLENEY TDRLSFTLNS ACYIKGDRAY LLYSTDGKSE EVSVRATSVF 

       190        200        210        220        230        240 
YLINELCGES AAYQDIIRCL IDNYPNTPIN KINQYVADLI DKEFLISNLR PPMTVSDQFQ 

       250        260        270        280        290        300 
YLIDQAESRH IPNELIQACK DIQYQIDAYN RITIGEGEHQ YLNLIETMNK LIKASSPLQV 

       310        320        330        340        350        360 
DAGLADSSIQ LDNETSLAIS ELASMFTYMA SPSANTLDHL EKYHNVFLER YGYEREVPLL 

       370        380        390        400        410        420 
EMLCSSTGIG APATYTNPAN EFFEETSFGE QFSPEMKQFF MRKYFESVRK KAPIQLDDET 

       430        440        450        460        470        480 
FHRICNSEIA DEEIPLSFEL NFFVKLRNGR VKLYLGPNVG STRAGKTFGR FSHMSDSISE 

       490        500        510        520        530        540 
IIKTLHNKEK ELTECNTKVC ELSIVPNQTR SGNVTRNVSY REKEMSLFTN SALHLNDSVK 

       550        560        570        580        590        600 
AEDILIGINK DHNFYARHKT TGEILSFESN HMFNPLLMTN AVRFLLEISR DGKRKWNDFP 

       610        620        630        640        650        660 
WFSIYSDFKY IPEIKYKEIT LSCEQWLIYK NDLSMHSNAS LEEIKSPFFE FHRTYELPQT 

       670        680        690        700        710        720 
FYIVNADNRL LIDIENDCTL DVFFWELKKT NHNQPLQLVA VEHDADALMD RNQNDYSGEI 

       730        740        750        760        770        780 
VVPLLRKQPE KPLYLPVLNA IEGSGSDRIK MPFEDWLFIK LYCKQTREEE LIAFEIADFY 

       790        800        810        820        830        840 
NQISDQYPVR HFFMRYRDPK PHIRLRFNGK AEVLYSLFPQ LLNWLKSLRE KGLVSESVIT 

       850        860        870        880        890        900 
QYEREIERYG GLSLMEAAEQ LFCEDSKVVE MMIRMHRMKD ITISKEIAGM VSVIQFLEQF 

       910        920        930        940        950        960 
ELTFEEQLTF LERNSLQNEY RTEFKKDREM YIEICNSDRD WDNLKKTSDG GMLYETLKTR 

       970        980        990       1000       1010       1020 
KMAAAHYAFL IKKAFDNKDE VYSRIGSIIH LHCNRLFGTD RELENKILTL CRHSLYAQRY 

      1030 
QKMNGSLAWK 

« Hide

References

[1]"Growth phase-dependent regulation and membrane localization of SpaB, a protein involved in biosynthesis of the lantibiotic subtilin."
Gutowski-Eckel Z., Klein C., Siegers K., Bohm K., Hammelmann M., Entian K.-D.
Appl. Environ. Microbiol. 60:1-11(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 6633 / PCI 219 / NRS 231.
[2]"Genes involved in self-protection against the lantibiotic subtilin produced by Bacillus subtilis ATCC 6633."
Klein C., Entian K.-D.
Appl. Environ. Microbiol. 60:2793-2801(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 6633 / PCI 219 / NRS 231.
[3]"Determination of the sequence of spaE and identification of a promoter in the subtilin (spa) operon in Bacillus subtilis."
Chung Y.J., Hansen J.N.
J. Bacteriol. 174:6699-6702(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 6633 / PCI 219 / NRS 231.
[4]"Analysis of genes involved in biosynthesis of the lantibiotic subtilin."
Klein C., Kaletta C., Schnell N., Entian K.-D.
Appl. Environ. Microbiol. 58:132-142(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 641-1030.
Strain: ATCC 6633 / PCI 219 / NRS 231.
[5]"The subtilin gene of Bacillus subtilis ATCC 6633 is encoded in an operon that contains a homolog of the hemolysin B transport protein."
Chung Y.J., Steen M.T., Hansen J.N.
J. Bacteriol. 174:1417-1422(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 852-1030.
Strain: ATCC 6633 / PCI 219 / NRS 231.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L24075 Genomic DNA. Translation: AAA22779.1.
U09819 Genomic DNA. Translation: AAB91586.1.
M99263 Genomic DNA. Translation: AAA22774.1. Frameshift.
M99263 Genomic DNA. Translation: AAA22775.1. Frameshift.
M86869 Genomic DNA. Translation: AAA22837.1.
M83944 Genomic DNA. Translation: AAA22769.1. Different initiation.
U38418 Genomic DNA. Translation: AAB01536.1.
PIRI39987.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-1521998.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR006827. Lant_deHydtase_C.
IPR006826. Lant_deHydtase_N.
IPR023809. Thiopep_bacteriocin_synth_dom.
[Graphical view]
PfamPF04738. Lant_dehyd_C. 1 hit.
PF04737. Lant_dehyd_N. 1 hit.
PF14028. SpaB_C. 1 hit.
[Graphical view]
TIGRFAMsTIGR03891. thiopep_ocin. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSPAB_BACIU
AccessionPrimary (citable) accession number: P39774
Secondary accession number(s): P33114, P36360
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 1, 1997
Last modified: May 29, 2013
This is version 54 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program