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P39774

- SPAB_BACIU

UniProt

P39774 - SPAB_BACIU

Protein

Subtilin biosynthesis protein SpaB

Gene

spaB

Organism
Bacillus subtilis
Status
Reviewed - Annotation score: 2 out of 5- Protein predictedi
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    • History
      Entry version 55 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Involved in the post-translational modification of the lantibiotic subtilin.

    GO - Biological processi

    1. transport Source: UniProtKB-KW

    Keywords - Biological processi

    Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Subtilin biosynthesis protein SpaB
    Gene namesi
    Name:spaB
    Synonyms:spaD, spaE
    OrganismiBacillus subtilis
    Taxonomic identifieri1423 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

    Subcellular locationi

    Cell membrane
    Note: Possibly associated with, and anchored to, the cytoplasmic side of the membrane.

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10301030Subtilin biosynthesis protein SpaBPRO_0000072091Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-1521998.

    Family & Domainsi

    Sequence similaritiesi

    To S.epidermidis EpiB and L.lactis NisB.Curated

    Family and domain databases

    InterProiIPR006827. Lant_deHydtase_C.
    IPR006826. Lant_deHydtase_N.
    IPR023809. Thiopep_bacteriocin_synth_dom.
    [Graphical view]
    PfamiPF04738. Lant_dehyd_C. 1 hit.
    PF04737. Lant_dehyd_N. 1 hit.
    PF14028. SpaB_C. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR03891. thiopep_ocin. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P39774-1 [UniParc]FASTAAdd to Basket

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    MKSLYTPTDY YMIRVPLVHQ DLKNENSQDI DQLLHDLCND SLFREQILVS     50
    SRTLYETIHT FLQAPDKLKG KKKRNFQQAI LKYATRRATR TTPFGLFSSV 100
    GIGSFSDKNH LSFNQHSFYK KARVDLEWLY QLIRKLENEY TDRLSFTLNS 150
    ACYIKGDRAY LLYSTDGKSE EVSVRATSVF YLINELCGES AAYQDIIRCL 200
    IDNYPNTPIN KINQYVADLI DKEFLISNLR PPMTVSDQFQ YLIDQAESRH 250
    IPNELIQACK DIQYQIDAYN RITIGEGEHQ YLNLIETMNK LIKASSPLQV 300
    DAGLADSSIQ LDNETSLAIS ELASMFTYMA SPSANTLDHL EKYHNVFLER 350
    YGYEREVPLL EMLCSSTGIG APATYTNPAN EFFEETSFGE QFSPEMKQFF 400
    MRKYFESVRK KAPIQLDDET FHRICNSEIA DEEIPLSFEL NFFVKLRNGR 450
    VKLYLGPNVG STRAGKTFGR FSHMSDSISE IIKTLHNKEK ELTECNTKVC 500
    ELSIVPNQTR SGNVTRNVSY REKEMSLFTN SALHLNDSVK AEDILIGINK 550
    DHNFYARHKT TGEILSFESN HMFNPLLMTN AVRFLLEISR DGKRKWNDFP 600
    WFSIYSDFKY IPEIKYKEIT LSCEQWLIYK NDLSMHSNAS LEEIKSPFFE 650
    FHRTYELPQT FYIVNADNRL LIDIENDCTL DVFFWELKKT NHNQPLQLVA 700
    VEHDADALMD RNQNDYSGEI VVPLLRKQPE KPLYLPVLNA IEGSGSDRIK 750
    MPFEDWLFIK LYCKQTREEE LIAFEIADFY NQISDQYPVR HFFMRYRDPK 800
    PHIRLRFNGK AEVLYSLFPQ LLNWLKSLRE KGLVSESVIT QYEREIERYG 850
    GLSLMEAAEQ LFCEDSKVVE MMIRMHRMKD ITISKEIAGM VSVIQFLEQF 900
    ELTFEEQLTF LERNSLQNEY RTEFKKDREM YIEICNSDRD WDNLKKTSDG 950
    GMLYETLKTR KMAAAHYAFL IKKAFDNKDE VYSRIGSIIH LHCNRLFGTD 1000
    RELENKILTL CRHSLYAQRY QKMNGSLAWK 1030
    Length:1,030
    Mass (Da):120,529
    Last modified:November 1, 1997 - v2
    Checksum:i89F3B147E02A9DF8
    GO

    Sequence cautioni

    The sequence AAA22774.1 differs from that shown. Reason: Frameshift at position 852.
    The sequence AAA22775.1 differs from that shown. Reason: Frameshift at position 852.
    The sequence AAA22769.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti126 – 1261L → F in AAA22774. (PubMed:1400221)Curated
    Sequence conflicti261 – 2611D → N in AAA22774. (PubMed:1400221)Curated
    Sequence conflicti304 – 3052LA → AG in AAA22774. (PubMed:1400221)Curated
    Sequence conflicti344 – 3441H → K in AAA22774. (PubMed:1400221)Curated
    Sequence conflicti553 – 5531N → I in AAA22774. (PubMed:1400221)Curated
    Sequence conflicti584 – 5841F → L in AAA22774. (PubMed:1400221)Curated
    Sequence conflicti647 – 6471P → A in AAA22774. (PubMed:1400221)Curated
    Sequence conflicti730 – 7301E → V in AAA22774. (PubMed:1400221)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L24075 Genomic DNA. Translation: AAA22779.1.
    U09819 Genomic DNA. Translation: AAB91586.1.
    M99263 Genomic DNA. Translation: AAA22774.1. Frameshift.
    M99263 Genomic DNA. Translation: AAA22775.1. Frameshift.
    M86869 Genomic DNA. Translation: AAA22837.1.
    M83944 Genomic DNA. Translation: AAA22769.1. Different initiation.
    U38418 Genomic DNA. Translation: AAB01536.1.
    PIRiI39987.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L24075 Genomic DNA. Translation: AAA22779.1 .
    U09819 Genomic DNA. Translation: AAB91586.1 .
    M99263 Genomic DNA. Translation: AAA22774.1 . Frameshift.
    M99263 Genomic DNA. Translation: AAA22775.1 . Frameshift.
    M86869 Genomic DNA. Translation: AAA22837.1 .
    M83944 Genomic DNA. Translation: AAA22769.1 . Different initiation.
    U38418 Genomic DNA. Translation: AAB01536.1 .
    PIRi I39987.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-1521998.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR006827. Lant_deHydtase_C.
    IPR006826. Lant_deHydtase_N.
    IPR023809. Thiopep_bacteriocin_synth_dom.
    [Graphical view ]
    Pfami PF04738. Lant_dehyd_C. 1 hit.
    PF04737. Lant_dehyd_N. 1 hit.
    PF14028. SpaB_C. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR03891. thiopep_ocin. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Growth phase-dependent regulation and membrane localization of SpaB, a protein involved in biosynthesis of the lantibiotic subtilin."
      Gutowski-Eckel Z., Klein C., Siegers K., Bohm K., Hammelmann M., Entian K.-D.
      Appl. Environ. Microbiol. 60:1-11(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 6633 / PCI 219 / NRS 231.
    2. "Genes involved in self-protection against the lantibiotic subtilin produced by Bacillus subtilis ATCC 6633."
      Klein C., Entian K.-D.
      Appl. Environ. Microbiol. 60:2793-2801(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 6633 / PCI 219 / NRS 231.
    3. "Determination of the sequence of spaE and identification of a promoter in the subtilin (spa) operon in Bacillus subtilis."
      Chung Y.J., Hansen J.N.
      J. Bacteriol. 174:6699-6702(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 6633 / PCI 219 / NRS 231.
    4. "Analysis of genes involved in biosynthesis of the lantibiotic subtilin."
      Klein C., Kaletta C., Schnell N., Entian K.-D.
      Appl. Environ. Microbiol. 58:132-142(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 641-1030.
      Strain: ATCC 6633 / PCI 219 / NRS 231.
    5. "The subtilin gene of Bacillus subtilis ATCC 6633 is encoded in an operon that contains a homolog of the hemolysin B transport protein."
      Chung Y.J., Steen M.T., Hansen J.N.
      J. Bacteriol. 174:1417-1422(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 852-1030.
      Strain: ATCC 6633 / PCI 219 / NRS 231.

    Entry informationi

    Entry nameiSPAB_BACIU
    AccessioniPrimary (citable) accession number: P39774
    Secondary accession number(s): P33114, P36360
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 55 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Caution

    Was originally proposed to code for two separate adjacent ORFs, SpaE and SpaD.1 Publication

    External Data

    Dasty 3