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Protein

Homeotic protein spalt-major

Gene

salm

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the establishment of the posterior-most head and the anterior-most tail segments of the embryo. Probably function as a transcriptional regulator. Could repress the transcription of the tsh gene.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri451 – 47323C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri479 – 50123C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri824 – 84623C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri852 – 87423C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri884 – 90623C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1289 – 131123C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1317 – 133923C2H2-type 7PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • antennal joint development Source: FlyBase
  • branched duct epithelial cell fate determination, open tracheal system Source: FlyBase
  • compound eye photoreceptor cell differentiation Source: FlyBase
  • epithelial cell migration, open tracheal system Source: FlyBase
  • glial cell development Source: FlyBase
  • imaginal disc-derived wing vein morphogenesis Source: FlyBase
  • male genitalia development Source: FlyBase
  • male gonad development Source: FlyBase
  • muscle organ morphogenesis Source: FlyBase
  • negative regulation of terminal cell fate specification, open tracheal system Source: FlyBase
  • negative regulation of transcription, DNA-templated Source: FlyBase
  • notum cell fate specification Source: FlyBase
  • oenocyte development Source: FlyBase
  • open tracheal system development Source: FlyBase
  • R7 cell differentiation Source: FlyBase
  • R8 cell differentiation Source: FlyBase
  • regulation of alternative mRNA splicing, via spliceosome Source: FlyBase
  • regulation of cell fate specification Source: FlyBase
  • regulation of transcription, DNA-templated Source: FlyBase
  • segment specification Source: FlyBase
  • sensory organ development Source: FlyBase
  • sensory perception of sound Source: FlyBase
  • somatic muscle development Source: FlyBase
  • spiracle morphogenesis, open tracheal system Source: FlyBase
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Homeotic protein spalt-major
Gene namesi
Name:salm
Synonyms:sal
ORF Names:CG6464
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0261648. salm.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13651365Homeotic protein spalt-majorPRO_0000047018Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei739 – 7391Phosphoserine1 Publication
Modified residuei744 – 7441Phosphoserine1 Publication
Modified residuei1076 – 10761Phosphoserine1 Publication
Modified residuei1079 – 10791Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP39770.

PTM databases

iPTMnetiP39770.

Expressioni

Developmental stagei

First expressed at blastoderm stage and later in restricted aeras of the embryonic nervous system as well as in the developing trachea.1 Publication

Gene expression databases

BgeeiP39770.
ExpressionAtlasiP39770. differential.
GenevisibleiP39770. DM.

Interactioni

Protein-protein interaction databases

BioGridi60626. 12 interactions.
IntActiP39770. 2 interactions.
MINTiMINT-1547918.
STRINGi7227.FBpp0088852.

Structurei

3D structure databases

ProteinModelPortaliP39770.
SMRiP39770. Positions 451-510, 823-916, 1285-1320.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 7 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri451 – 47323C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri479 – 50123C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri824 – 84623C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri852 – 87423C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri884 – 90623C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1289 – 131123C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1317 – 133923C2H2-type 7PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1074. Eukaryota.
ENOG410ZE3Z. LUCA.
GeneTreeiENSGT00550000074555.
InParanoidiP39770.
KOiK19871.
OMAiSMMPGHF.
OrthoDBiEOG7CNZF2.
PhylomeDBiP39770.

Family and domain databases

Gene3Di3.30.160.60. 7 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 2 hits.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 7 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 7 hits.
PS50157. ZINC_FINGER_C2H2_2. 7 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39770-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNHLSNVLC AMRSDFKDNH QETINKMIQF GTVKYGIVKQ LKDRARSADK
60 70 80 90 100
DIGSDQEENG GCSPLTTATT TASPSRSPEP EEEQPEEQST SEQSIPEQST
110 120 130 140 150
PDHQLENDIK SEAKSEIEPV EDNNNRVAMT KPSSEEREPN ASGSMPSSPV
160 170 180 190 200
AEASAEEAAT ERTPEKEKEK DVEVDVEKPD EAPSSAVPST EVTLPGGAGA
210 220 230 240 250
PVTLEAIQNM QMAIAQFAAK TIANGSNGAD NEAAMKQLAF LQQTLFNLQQ
260 270 280 290 300
QQLFQIQLIQ QLQSQLALNQ AKQEEDTEED ADQEQDQEQE TDTYEEEERI
310 320 330 340 350
ADMELRQKAE ARMAEAKARQ HLINAGVPLR ESSGSPAESL KRRREHDHES
360 370 380 390 400
QPNRRPSLDN THKADTAQDA LAKLKEMENT PLPFGSDLAS SIITNHDDLP
410 420 430 440 450
EPNSLDLLQK RAQEVLDSAS QGILANSMAD DFAFGEKSGE GKGRNEPFFK
460 470 480 490 500
HRCRYCGKVF GSDSALQIHI RSHTGERPFK CNVCGSRFTT KGNLKVHFQR
510 520 530 540 550
HAQKFPHVPM NATPIPEHMD KFHPPLLDQM SPTDSSPNHS PAPPPLGSAP
560 570 580 590 600
ASFPPAFPGL QNLYRPPMEI LKSLGAAAPH QYFPQELPTD LRKPSPQLDE
610 620 630 640 650
DEPQVKNEPV EEKDQREEHE QEMAECSEPE PEPLPLEVRI KEERVEEQEQ
660 670 680 690 700
VKQEDHRIEP RRTPSPSSEH RSPHHHRHSH MGYPPVVQPI QPAALMHPQS
710 720 730 740 750
SPGSQSHLDH LPTPGQLPPR EDFFAERFPL NFTTAKMLSP EHHSPVRSPA
760 770 780 790 800
GGALPPGVPP PPHHHPHHMA RSPFFNPIKH EMAALLPRPH SNDNSWENFI
810 820 830 840 850
EVSNTCETMK LKELMKNKKI SDPNQCVVCD RVLSCKSALQ MHYRTHTGER
860 870 880 890 900
PFKCRICGRA FTTKGNLKTH MAVHKIRPPM RNFHQCPVCH KKYSNALVLQ
910 920 930 940 950
QHIRLHTGEP TDLTPEQIQA AEIRDPPPSM MPGHFMNPFA AAAFHFGALP
960 970 980 990 1000
GGPGGPPGPN HGAHNGALGS ESSQGDMDDN MDCGEDYDDD VSSEHLSNSN
1010 1020 1030 1040 1050
LEQEGDRSRS GDDFKSLLFE QKLRIDATGV VNTNPVRPRS SASSHGHSVG
1060 1070 1080 1090 1100
STSAPTSPSV HASSQVIKRS SSPARSEASQ GALDLTPRAA PTSSSSSRSP
1110 1120 1130 1140 1150
LPKEKPVSPP SLPRSPSGSS HASANILTSP LPPTVGIDCL PPGLQHHLQQ
1160 1170 1180 1190 1200
QHQHLMQQQA AVAAAAAAQH HHHQQMAALH QHQEQLRREA AEAQQKAAAA
1210 1220 1230 1240 1250
AAAAAAAAAA QRQTPPQARD QRQEGGPGAG PPPNPLMGAR PPFGMFPNLP
1260 1270 1280 1290 1300
LFPPATTQNM CNAMNQIAQS VMPAAPFNPL ALSGVRGSTT CGICYKTFPC
1310 1320 1330 1340 1350
HSALEIHYRS HTKERPFKCS ICDRGFTTKG NLKQHMLTHK IRDMEQETFR
1360
NRAVKYMSEW NEDRE
Length:1,365
Mass (Da):150,319
Last modified:August 31, 2004 - v3
Checksum:iBDF5896C43CD016A
GO

Sequence cautioni

The sequence AAM50766.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAA53229.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti178 – 1781K → M in CAA53229 (PubMed:7905822).Curated
Sequence conflicti356 – 3561P → T in CAA53229 (PubMed:7905822).Curated
Sequence conflicti1142 – 11421P → K in CAA53229 (PubMed:7905822).Curated
Sequence conflicti1180 – 11801H → D in CAA53229 (PubMed:7905822).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75541 Genomic DNA. Translation: CAA53229.1. Sequence problems.
AE014134 Genomic DNA. Translation: AAF53097.3.
AY118906 mRNA. Translation: AAM50766.1. Different initiation.
PIRiS40022.
RefSeqiNP_723670.2. NM_164966.3.

Genome annotation databases

EnsemblMetazoaiFBtr0089913; FBpp0088852; FBgn0261648.
GeneIDi34569.
KEGGidme:Dmel_CG6464.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75541 Genomic DNA. Translation: CAA53229.1. Sequence problems.
AE014134 Genomic DNA. Translation: AAF53097.3.
AY118906 mRNA. Translation: AAM50766.1. Different initiation.
PIRiS40022.
RefSeqiNP_723670.2. NM_164966.3.

3D structure databases

ProteinModelPortaliP39770.
SMRiP39770. Positions 451-510, 823-916, 1285-1320.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi60626. 12 interactions.
IntActiP39770. 2 interactions.
MINTiMINT-1547918.
STRINGi7227.FBpp0088852.

PTM databases

iPTMnetiP39770.

Proteomic databases

PaxDbiP39770.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0089913; FBpp0088852; FBgn0261648.
GeneIDi34569.
KEGGidme:Dmel_CG6464.

Organism-specific databases

CTDi34569.
FlyBaseiFBgn0261648. salm.

Phylogenomic databases

eggNOGiKOG1074. Eukaryota.
ENOG410ZE3Z. LUCA.
GeneTreeiENSGT00550000074555.
InParanoidiP39770.
KOiK19871.
OMAiSMMPGHF.
OrthoDBiEOG7CNZF2.
PhylomeDBiP39770.

Miscellaneous databases

ChiTaRSisls. fly.
GenomeRNAii34569.
PROiP39770.

Gene expression databases

BgeeiP39770.
ExpressionAtlasiP39770. differential.
GenevisibleiP39770. DM.

Family and domain databases

Gene3Di3.30.160.60. 7 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 2 hits.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 7 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 7 hits.
PS50157. ZINC_FINGER_C2H2_2. 7 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Spalt encodes an evolutionarily conserved zinc finger protein of novel structure which provides homeotic gene function in the head and tail region of the Drosophila embryo."
    Kuehnlein R.P., Frommer G., Friedrich M., Gonzalez-Gaitan M., Weber A., Wagner-Bernholz J.F., Gehring W.J., Jaeckle H., Schuh R.
    EMBO J. 13:168-179(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DEVELOPMENTAL STAGE.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. "A Drosophila full-length cDNA resource."
    Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
    Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 855-1365.
    Strain: Berkeley.
    Tissue: Embryo.
  5. "Phosphoproteome analysis of Drosophila melanogaster embryos."
    Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
    J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-739; SER-744; SER-1076 AND SER-1079, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Embryo.

Entry informationi

Entry nameiSALM_DROME
AccessioniPrimary (citable) accession number: P39770
Secondary accession number(s): Q8MSC6, Q9VKH2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: August 31, 2004
Last modified: June 8, 2016
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.