Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Flap endonuclease 1

Gene

rad2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi34Magnesium 1UniRule annotation1
Binding sitei47DNA substrateUniRule annotation1
Binding sitei71DNA substrateUniRule annotation1
Metal bindingi87Magnesium 1UniRule annotation1
Metal bindingi159Magnesium 1UniRule annotation1
Binding sitei159DNA substrateUniRule annotation1
Metal bindingi161Magnesium 1UniRule annotation1
Metal bindingi180Magnesium 2UniRule annotation1
Metal bindingi182Magnesium 2UniRule annotation1
Binding sitei232DNA substrateUniRule annotation1
Metal bindingi234Magnesium 2UniRule annotation1
Binding sitei234DNA substrateUniRule annotation1

GO - Molecular functioni

  • 5'-3' exodeoxyribonuclease activity involved in UV-damage excision repair Source: PomBase
  • 5'-flap endonuclease activity Source: PomBase
  • DNA binding Source: InterPro
  • double-stranded DNA 5'-3' exodeoxyribonuclease activity Source: PomBase
  • metal ion binding Source: UniProtKB-KW
  • single-stranded DNA 5'-3' exodeoxyribonuclease activity Source: PomBase

GO - Biological processi

  • DNA synthesis involved in DNA repair Source: PomBase
  • removal of RNA primer involved in mitotic DNA replication Source: PomBase

Keywordsi

Molecular functionEndonuclease, Exonuclease, Hydrolase, Nuclease
Biological processDNA damage, DNA repair, DNA replication
LigandMagnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-SPO-174437 Removal of the Flap Intermediate from the C-strand
R-SPO-5651801 PCNA-Dependent Long Patch Base Excision Repair
R-SPO-69166 Removal of the Flap Intermediate

Names & Taxonomyi

Protein namesi
Recommended name:
Flap endonuclease 1UniRule annotation (EC:3.1.-.-UniRule annotation)
Short name:
FEN-1UniRule annotation
Alternative name(s):
DNA repair protein rad2
Flap structure-specific endonuclease 1UniRule annotation
Gene namesi
Name:rad2UniRule annotation
Synonyms:fen1UniRule annotation
ORF Names:SPAC3G6.06c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC3G6.06c
PomBaseiSPAC3G6.06c rad2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001540371 – 380Flap endonuclease 1Add BLAST380

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei350Phosphoserine1 Publication1
Modified residuei351Phosphoserine1 Publication1

Post-translational modificationi

Phosphorylated. Phosphorylation upon DNA damage induces relocalization to the nuclear plasma.UniRule annotation

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP39750
PaxDbiP39750
PRIDEiP39750

PTM databases

iPTMnetiP39750

Interactioni

Subunit structurei

Interacts with PCNA. Three molecules of rad2 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity.UniRule annotation

Protein-protein interaction databases

BioGridi279595, 102 interactors
STRINGi4896.SPAC3G6.06c.1

Structurei

3D structure databases

ProteinModelPortaliP39750
SMRiP39750
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 105N-domainAdd BLAST105
Regioni123 – 254I-domainAdd BLAST132
Regioni337 – 345Interaction with PCNAUniRule annotation9

Sequence similaritiesi

Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000193853
InParanoidiP39750
KOiK04799
OMAiGSQDYDS
OrthoDBiEOG092C2ISI
PhylomeDBiP39750

Family and domain databases

CDDicd09867 PIN_FEN1, 1 hit
HAMAPiMF_00614 Fen, 1 hit
InterProiView protein in InterPro
IPR036279 5-3_exonuclease_C_sf
IPR023426 Flap_endonuc
IPR008918 HhH2
IPR029060 PIN-like_dom_sf
IPR006086 XPG-I_dom
IPR006084 XPG/Rad2
IPR019974 XPG_CS
IPR006085 XPG_DNA_repair_N
PANTHERiPTHR11081 PTHR11081, 1 hit
PfamiView protein in Pfam
PF00867 XPG_I, 1 hit
PF00752 XPG_N, 1 hit
PRINTSiPR00853 XPGRADSUPER
SMARTiView protein in SMART
SM00279 HhH2, 1 hit
SM00484 XPGI, 1 hit
SM00485 XPGN, 1 hit
SUPFAMiSSF47807 SSF47807, 1 hit
SSF88723 SSF88723, 1 hit
PROSITEiView protein in PROSITE
PS00841 XPG_1, 1 hit
PS00842 XPG_2, 1 hit

Sequencei

Sequence statusi: Complete.

P39750-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGIKGLAQVL SEHAPASVKH NDIKNYFGRK VAIDASMSLY QFLIQVRSQD
60 70 80 90 100
GQQLMNEQGE TTSHLMGMFY RTLRIVDNGI KPCFVFDGKP PTLKSGELAK
110 120 130 140 150
RVARHQKARE DQEETKEVGT AEMVDRFAKR TVKVTRQHND EAKRLLELMG
160 170 180 190 200
IPFVNAPCEA EAQCAALARS GKVYAAASED MDTLCFQAPV LLRHLTFSEQ
210 220 230 240 250
RKEPISEYNI EKALNGLDMS VEQFVDLCIL LGCDYCEPIR GVGPARAVEL
260 270 280 290 300
IRQYGTLDRF VKEADRSKYP IPEDWPYEDA RRLFLDAEVL PGEEIELKWK
310 320 330 340 350
SPDADGIIQF LVKEKGFNED RVKLGINRLE KASKTIPQGR LDSFFKPVPS
360 370 380
SPKKPVDTKS KGSAKRKRDS NKGGESKKKR
Length:380
Mass (Da):42,866
Last modified:February 1, 1995 - v1
Checksum:i3604065D9B934CBF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77041 Genomic DNA Translation: CAB36991.1
CU329670 Genomic DNA Translation: CAB16282.1
PIRiA56054
RefSeqiNP_594972.1, NM_001020403.2

Genome annotation databases

EnsemblFungiiSPAC3G6.06c.1; SPAC3G6.06c.1:pep; SPAC3G6.06c
GeneIDi2543164
KEGGispo:SPAC3G6.06c

Entry informationi

Entry nameiFEN1_SCHPO
AccessioniPrimary (citable) accession number: P39750
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: March 28, 2018
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health