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P39745 (SUR1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase mpk-1

EC=2.7.11.24
Alternative name(s):
MAP kinase sur-1
Gene names
Name:mpk-1
Synonyms:sur-1
ORF Names:F43C1.2
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length444 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Function in let-60 Ras signaling pathway; acts downstream of lin-45 raf kinase, but before the lin-1 gene product in controlling vulval cell differentiation.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium By similarity.

Enzyme regulation

Activated by tyrosine and threonine phosphorylation By similarity.

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-256 and Tyr-258, which activates the enzyme By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

gla-3O022893EBI-321013,EBI-317795

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform b (identifier: P39745-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform a (identifier: P39745-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-68: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 444444Mitogen-activated protein kinase mpk-1
PRO_0000186306

Regions

Domain96 – 384289Protein kinase
Nucleotide binding102 – 1109ATP By similarity
Motif256 – 2583TXY

Sites

Active site2201Proton acceptor By similarity
Binding site1251ATP By similarity

Amino acid modifications

Modified residue2561Phosphothreonine By similarity
Modified residue2581Phosphotyrosine By similarity

Natural variations

Alternative sequence1 – 6868Missing in isoform a.
VSP_004848

Experimental info

Mutagenesis1061A → V: Loss of function and ATP-binding.
Sequence conflict9 – 102LC → FF in AAA18956. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform b [UniParc].

Last modified November 25, 2002. Version 2.
Checksum: 11BA27D17641980D

FASTA44450,663
        10         20         30         40         50         60 
MPTWIPNNLC AQPTTRNAKP PSNGHPQATQ QQSAPGSLAY RNSSNIPNGA TNHVRQQKWQ 

        70         80         90        100        110        120 
YTRSGHRKMA DGEAVISTVN NVEEVHGQLF EVAPRYVNLS YIGEGAYGMV ASALDTITRD 

       130        140        150        160        170        180 
RVAIKKISPF EHQTFCQRTL REIKILNRFK HENIINIQEI IRSETVDSLK DIYIVQCLME 

       190        200        210        220        230        240 
TDLYKLLKTQ KLSNDHVCYF LYQILRGLKY IHSANVLHRD LKPSNLLLNT TCDLKICDFG 

       250        260        270        280        290        300 
LARVTDPQTD HTGFLTEYVA TRWYRAPEIM LNSKGYTKSI DVWSVGCILA EMLSNRPLFP 

       310        320        330        340        350        360 
GKHYLDQLNL ILAVVGSPSN ADLQCIINDK ARSYLISLPH KPKQPWARLY PGADPRALDL 

       370        380        390        400        410        420 
LDKMLTFNPH NRIDIEQALA HPYLEQYYDP GDEPVCEEPF TLEMEFDDLP KEKLKELIWE 

       430        440 
EAEAHHRRME AEAAARNNGG QNPV 

« Hide

Isoform a [UniParc].

Checksum: 9974B791EFE16838
Show »

FASTA37643,102

References

« Hide 'large scale' references
[1]"Suppression of activated Let-60 ras protein defines a role of Caenorhabditis elegans Sur-1 MAP kinase in vulval differentiation."
Wu Y., Han M.
Genes Dev. 8:147-159(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
Strain: Bristol N2.
[2]"A MAP kinase homolog, mpk-1, is involved in ras-mediated induction of vulval cell fates in Caenorhabditis elegans."
Lackner M.R., Kornfeld K., Miller L.M., Horvitz H.R., Kim S.K.
Genes Dev. 8:160-173(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
Strain: Bristol N2.
[3]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U03879 mRNA. Translation: AAA18956.1.
U27124 mRNA. Translation: AAA73482.1.
Z46937 Genomic DNA. Translation: CAA87057.1.
Z46937 Genomic DNA. Translation: CAB60996.1.
PIRA36977.
A36978.
RefSeqNP_001022583.1. NM_001027412.3. [P39745-2]
NP_001022584.1. NM_001027413.2. [P39745-1]
UniGeneCel.34032.

3D structure databases

ProteinModelPortalP39745.
SMRP39745. Positions 90-421.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid40782. 65 interactions.
DIPDIP-26227N.
IntActP39745. 51 interactions.
MINTMINT-114245.
STRING6239.F43C1.2b.

Proteomic databases

PaxDbP39745.
PRIDEP39745.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaF43C1.2b; F43C1.2b; WBGene00003401. [P39745-1]
GeneID175545.
KEGGcel:CELE_F43C1.2.
UCSCF43C1.2a.1. c. elegans. [P39745-1]

Organism-specific databases

CTD175545.
WormBaseF43C1.2a; CE01583; WBGene00003401; mpk-1.
F43C1.2b; CE24971; WBGene00003401; mpk-1.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00550000074298.
HOGENOMHOG000233024.
InParanoidP39745.
KOK04371.
OMAHAYLSPH.
OrthoDBEOG7M3J0K.
PhylomeDBP39745.

Enzyme and pathway databases

BRENDA2.7.11.24. 1045.
SignaLinkP39745.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008349. MAPK_ERK1/2.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSPR01770. ERK1ERK2MAPK.
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio888610.
PROP39745.

Entry information

Entry nameSUR1_CAEEL
AccessionPrimary (citable) accession number: P39745
Secondary accession number(s): Q9U3F3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 25, 2002
Last modified: July 9, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase