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P39745

- SUR1_CAEEL

UniProt

P39745 - SUR1_CAEEL

Protein

Mitogen-activated protein kinase mpk-1

Gene

mpk-1

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 2 (25 Nov 2002)
      Previous versions | rss
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    Functioni

    Function in let-60 Ras signaling pathway; acts downstream of lin-45 raf kinase, but before the lin-1 gene product in controlling vulval cell differentiation.

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    Activated by tyrosine and threonine phosphorylation.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei125 – 1251ATPPROSITE-ProRule annotation
    Active sitei220 – 2201Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi102 – 1109ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. MAP kinase activity Source: UniProtKB
    3. protein binding Source: IntAct
    4. protein serine/threonine kinase activity Source: WormBase

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. MAPK cascade Source: GOC
    3. oocyte maturation Source: WormBase
    4. peptidyl-serine phosphorylation Source: WormBase
    5. Ras protein signal transduction Source: WormBase
    6. vulval development Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.24. 1045.
    ReactomeiREACT_183011. RSK activation.
    REACT_183032. CREB phosphorylation through the activation of Ras.
    REACT_183795. ERKs are inactivated.
    REACT_183805. Senescence-Associated Secretory Phenotype (SASP).
    REACT_184116. Gastrin-CREB signalling pathway via PKC and MAPK.
    REACT_205771. Regulation of HSF1-mediated heat shock response.
    REACT_207352. Signal attenuation.
    SignaLinkiP39745.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase mpk-1 (EC:2.7.11.24)
    Alternative name(s):
    MAP kinase sur-1
    Gene namesi
    Name:mpk-1
    Synonyms:sur-1
    ORF Names:F43C1.2
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome III

    Organism-specific databases

    WormBaseiF43C1.2a; CE01583; WBGene00003401; mpk-1.
    F43C1.2b; CE24971; WBGene00003401; mpk-1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: WormBase
    2. nucleus Source: WormBase

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi106 – 1061A → V: Loss of function and ATP-binding.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 444444Mitogen-activated protein kinase mpk-1PRO_0000186306Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei256 – 2561PhosphothreonineBy similarity
    Modified residuei258 – 2581PhosphotyrosineBy similarity

    Post-translational modificationi

    Dually phosphorylated on Thr-256 and Tyr-258, which activates the enzyme.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP39745.
    PRIDEiP39745.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    gla-3O022893EBI-321013,EBI-317795

    Protein-protein interaction databases

    BioGridi40782. 66 interactions.
    DIPiDIP-26227N.
    IntActiP39745. 51 interactions.
    MINTiMINT-114245.
    STRINGi6239.F43C1.2b.

    Structurei

    3D structure databases

    ProteinModelPortaliP39745.
    SMRiP39745. Positions 90-421.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini96 – 384289Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi256 – 2583TXY

    Domaini

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00550000074298.
    HOGENOMiHOG000233024.
    InParanoidiP39745.
    KOiK04371.
    OMAiHAYLSPH.
    OrthoDBiEOG7M3J0K.
    PhylomeDBiP39745.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR008349. MAPK_ERK1/2.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    PRINTSiPR01770. ERK1ERK2MAPK.
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform b (identifier: P39745-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MPTWIPNNLC AQPTTRNAKP PSNGHPQATQ QQSAPGSLAY RNSSNIPNGA    50
    TNHVRQQKWQ YTRSGHRKMA DGEAVISTVN NVEEVHGQLF EVAPRYVNLS 100
    YIGEGAYGMV ASALDTITRD RVAIKKISPF EHQTFCQRTL REIKILNRFK 150
    HENIINIQEI IRSETVDSLK DIYIVQCLME TDLYKLLKTQ KLSNDHVCYF 200
    LYQILRGLKY IHSANVLHRD LKPSNLLLNT TCDLKICDFG LARVTDPQTD 250
    HTGFLTEYVA TRWYRAPEIM LNSKGYTKSI DVWSVGCILA EMLSNRPLFP 300
    GKHYLDQLNL ILAVVGSPSN ADLQCIINDK ARSYLISLPH KPKQPWARLY 350
    PGADPRALDL LDKMLTFNPH NRIDIEQALA HPYLEQYYDP GDEPVCEEPF 400
    TLEMEFDDLP KEKLKELIWE EAEAHHRRME AEAAARNNGG QNPV 444
    Length:444
    Mass (Da):50,663
    Last modified:November 25, 2002 - v2
    Checksum:i11BA27D17641980D
    GO
    Isoform a (identifier: P39745-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-68: Missing.

    Show »
    Length:376
    Mass (Da):43,102
    Checksum:i9974B791EFE16838
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti9 – 102LC → FF in AAA18956. (PubMed:8299936)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 6868Missing in isoform a. 1 PublicationVSP_004848Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U03879 mRNA. Translation: AAA18956.1.
    U27124 mRNA. Translation: AAA73482.1.
    Z46937 Genomic DNA. Translation: CAA87057.1.
    Z46937 Genomic DNA. Translation: CAB60996.1.
    PIRiA36977.
    A36978.
    RefSeqiNP_001022583.1. NM_001027412.3. [P39745-2]
    NP_001022584.1. NM_001027413.2. [P39745-1]
    UniGeneiCel.34032.

    Genome annotation databases

    EnsemblMetazoaiF43C1.2b; F43C1.2b; WBGene00003401. [P39745-1]
    GeneIDi175545.
    KEGGicel:CELE_F43C1.2.
    UCSCiF43C1.2a.1. c. elegans. [P39745-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U03879 mRNA. Translation: AAA18956.1 .
    U27124 mRNA. Translation: AAA73482.1 .
    Z46937 Genomic DNA. Translation: CAA87057.1 .
    Z46937 Genomic DNA. Translation: CAB60996.1 .
    PIRi A36977.
    A36978.
    RefSeqi NP_001022583.1. NM_001027412.3. [P39745-2 ]
    NP_001022584.1. NM_001027413.2. [P39745-1 ]
    UniGenei Cel.34032.

    3D structure databases

    ProteinModelPortali P39745.
    SMRi P39745. Positions 90-421.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 40782. 66 interactions.
    DIPi DIP-26227N.
    IntActi P39745. 51 interactions.
    MINTi MINT-114245.
    STRINGi 6239.F43C1.2b.

    Proteomic databases

    PaxDbi P39745.
    PRIDEi P39745.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai F43C1.2b ; F43C1.2b ; WBGene00003401 . [P39745-1 ]
    GeneIDi 175545.
    KEGGi cel:CELE_F43C1.2.
    UCSCi F43C1.2a.1. c. elegans. [P39745-1 ]

    Organism-specific databases

    CTDi 175545.
    WormBasei F43C1.2a ; CE01583 ; WBGene00003401 ; mpk-1.
    F43C1.2b ; CE24971 ; WBGene00003401 ; mpk-1.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00550000074298.
    HOGENOMi HOG000233024.
    InParanoidi P39745.
    KOi K04371.
    OMAi HAYLSPH.
    OrthoDBi EOG7M3J0K.
    PhylomeDBi P39745.

    Enzyme and pathway databases

    BRENDAi 2.7.11.24. 1045.
    Reactomei REACT_183011. RSK activation.
    REACT_183032. CREB phosphorylation through the activation of Ras.
    REACT_183795. ERKs are inactivated.
    REACT_183805. Senescence-Associated Secretory Phenotype (SASP).
    REACT_184116. Gastrin-CREB signalling pathway via PKC and MAPK.
    REACT_205771. Regulation of HSF1-mediated heat shock response.
    REACT_207352. Signal attenuation.
    SignaLinki P39745.

    Miscellaneous databases

    NextBioi 888610.
    PROi P39745.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR008349. MAPK_ERK1/2.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    PRINTSi PR01770. ERK1ERK2MAPK.
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Suppression of activated Let-60 ras protein defines a role of Caenorhabditis elegans Sur-1 MAP kinase in vulval differentiation."
      Wu Y., Han M.
      Genes Dev. 8:147-159(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
      Strain: Bristol N2.
    2. "A MAP kinase homolog, mpk-1, is involved in ras-mediated induction of vulval cell fates in Caenorhabditis elegans."
      Lackner M.R., Kornfeld K., Miller L.M., Horvitz H.R., Kim S.K.
      Genes Dev. 8:160-173(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
      Strain: Bristol N2.
    3. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.

    Entry informationi

    Entry nameiSUR1_CAEEL
    AccessioniPrimary (citable) accession number: P39745
    Secondary accession number(s): Q9U3F3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: November 25, 2002
    Last modified: October 1, 2014
    This is version 134 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3