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P39745

- SUR1_CAEEL

UniProt

P39745 - SUR1_CAEEL

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Protein

Mitogen-activated protein kinase mpk-1

Gene

mpk-1

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Function in let-60 Ras signaling pathway; acts downstream of lin-45 raf kinase, but before the lin-1 gene product in controlling vulval cell differentiation.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Magnesium.By similarity

Enzyme regulationi

Activated by tyrosine and threonine phosphorylation.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei125 – 1251ATPPROSITE-ProRule annotation
Active sitei220 – 2201Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi102 – 1109ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: UniProtKB
  3. protein serine/threonine kinase activity Source: WormBase

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. MAPK cascade Source: GOC
  3. oocyte maturation Source: WormBase
  4. peptidyl-serine phosphorylation Source: WormBase
  5. Ras protein signal transduction Source: WormBase
  6. vulval development Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.24. 1045.
ReactomeiREACT_183011. RSK activation.
REACT_183032. CREB phosphorylation through the activation of Ras.
REACT_183795. ERKs are inactivated.
REACT_183805. Senescence-Associated Secretory Phenotype (SASP).
REACT_184116. Gastrin-CREB signalling pathway via PKC and MAPK.
REACT_205771. Regulation of HSF1-mediated heat shock response.
REACT_207352. Signal attenuation.
SignaLinkiP39745.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase mpk-1 (EC:2.7.11.24)
Alternative name(s):
MAP kinase sur-1
Gene namesi
Name:mpk-1
Synonyms:sur-1
ORF Names:F43C1.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome III

Organism-specific databases

WormBaseiF43C1.2a; CE01583; WBGene00003401; mpk-1.
F43C1.2b; CE24971; WBGene00003401; mpk-1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: WormBase
  2. nucleus Source: WormBase
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi106 – 1061A → V: Loss of function and ATP-binding.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 444444Mitogen-activated protein kinase mpk-1PRO_0000186306Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei256 – 2561PhosphothreonineBy similarity
Modified residuei258 – 2581PhosphotyrosineBy similarity

Post-translational modificationi

Dually phosphorylated on Thr-256 and Tyr-258, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP39745.
PRIDEiP39745.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
gla-3O022893EBI-321013,EBI-317795

Protein-protein interaction databases

BioGridi40782. 66 interactions.
DIPiDIP-26227N.
IntActiP39745. 51 interactions.
MINTiMINT-114245.
STRINGi6239.F43C1.2b.

Structurei

3D structure databases

ProteinModelPortaliP39745.
SMRiP39745. Positions 90-421.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini96 – 384289Protein kinasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi256 – 2583TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00550000074298.
HOGENOMiHOG000233024.
InParanoidiP39745.
KOiK04371.
OMAiHAYLSPH.
OrthoDBiEOG7M3J0K.
PhylomeDBiP39745.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008349. MAPK_ERK1/2.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01770. ERK1ERK2MAPK.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform b (identifier: P39745-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPTWIPNNLC AQPTTRNAKP PSNGHPQATQ QQSAPGSLAY RNSSNIPNGA
60 70 80 90 100
TNHVRQQKWQ YTRSGHRKMA DGEAVISTVN NVEEVHGQLF EVAPRYVNLS
110 120 130 140 150
YIGEGAYGMV ASALDTITRD RVAIKKISPF EHQTFCQRTL REIKILNRFK
160 170 180 190 200
HENIINIQEI IRSETVDSLK DIYIVQCLME TDLYKLLKTQ KLSNDHVCYF
210 220 230 240 250
LYQILRGLKY IHSANVLHRD LKPSNLLLNT TCDLKICDFG LARVTDPQTD
260 270 280 290 300
HTGFLTEYVA TRWYRAPEIM LNSKGYTKSI DVWSVGCILA EMLSNRPLFP
310 320 330 340 350
GKHYLDQLNL ILAVVGSPSN ADLQCIINDK ARSYLISLPH KPKQPWARLY
360 370 380 390 400
PGADPRALDL LDKMLTFNPH NRIDIEQALA HPYLEQYYDP GDEPVCEEPF
410 420 430 440
TLEMEFDDLP KEKLKELIWE EAEAHHRRME AEAAARNNGG QNPV
Length:444
Mass (Da):50,663
Last modified:November 25, 2002 - v2
Checksum:i11BA27D17641980D
GO
Isoform a (identifier: P39745-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-68: Missing.

Show »
Length:376
Mass (Da):43,102
Checksum:i9974B791EFE16838
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti9 – 102LC → FF in AAA18956. (PubMed:8299936)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 6868Missing in isoform a. 1 PublicationVSP_004848Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U03879 mRNA. Translation: AAA18956.1.
U27124 mRNA. Translation: AAA73482.1.
Z46937 Genomic DNA. Translation: CAA87057.1.
Z46937 Genomic DNA. Translation: CAB60996.1.
PIRiA36977.
A36978.
RefSeqiNP_001022583.1. NM_001027412.3. [P39745-2]
NP_001022584.1. NM_001027413.2. [P39745-1]
UniGeneiCel.34032.

Genome annotation databases

EnsemblMetazoaiF43C1.2b; F43C1.2b; WBGene00003401. [P39745-1]
GeneIDi175545.
KEGGicel:CELE_F43C1.2.
UCSCiF43C1.2a.1. c. elegans. [P39745-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U03879 mRNA. Translation: AAA18956.1 .
U27124 mRNA. Translation: AAA73482.1 .
Z46937 Genomic DNA. Translation: CAA87057.1 .
Z46937 Genomic DNA. Translation: CAB60996.1 .
PIRi A36977.
A36978.
RefSeqi NP_001022583.1. NM_001027412.3. [P39745-2 ]
NP_001022584.1. NM_001027413.2. [P39745-1 ]
UniGenei Cel.34032.

3D structure databases

ProteinModelPortali P39745.
SMRi P39745. Positions 90-421.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 40782. 66 interactions.
DIPi DIP-26227N.
IntActi P39745. 51 interactions.
MINTi MINT-114245.
STRINGi 6239.F43C1.2b.

Proteomic databases

PaxDbi P39745.
PRIDEi P39745.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai F43C1.2b ; F43C1.2b ; WBGene00003401 . [P39745-1 ]
GeneIDi 175545.
KEGGi cel:CELE_F43C1.2.
UCSCi F43C1.2a.1. c. elegans. [P39745-1 ]

Organism-specific databases

CTDi 175545.
WormBasei F43C1.2a ; CE01583 ; WBGene00003401 ; mpk-1.
F43C1.2b ; CE24971 ; WBGene00003401 ; mpk-1.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00550000074298.
HOGENOMi HOG000233024.
InParanoidi P39745.
KOi K04371.
OMAi HAYLSPH.
OrthoDBi EOG7M3J0K.
PhylomeDBi P39745.

Enzyme and pathway databases

BRENDAi 2.7.11.24. 1045.
Reactomei REACT_183011. RSK activation.
REACT_183032. CREB phosphorylation through the activation of Ras.
REACT_183795. ERKs are inactivated.
REACT_183805. Senescence-Associated Secretory Phenotype (SASP).
REACT_184116. Gastrin-CREB signalling pathway via PKC and MAPK.
REACT_205771. Regulation of HSF1-mediated heat shock response.
REACT_207352. Signal attenuation.
SignaLinki P39745.

Miscellaneous databases

NextBioi 888610.
PROi P39745.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008349. MAPK_ERK1/2.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
PRINTSi PR01770. ERK1ERK2MAPK.
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Suppression of activated Let-60 ras protein defines a role of Caenorhabditis elegans Sur-1 MAP kinase in vulval differentiation."
    Wu Y., Han M.
    Genes Dev. 8:147-159(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
    Strain: Bristol N2.
  2. "A MAP kinase homolog, mpk-1, is involved in ras-mediated induction of vulval cell fates in Caenorhabditis elegans."
    Lackner M.R., Kornfeld K., Miller L.M., Horvitz H.R., Kim S.K.
    Genes Dev. 8:160-173(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
    Strain: Bristol N2.
  3. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiSUR1_CAEEL
AccessioniPrimary (citable) accession number: P39745
Secondary accession number(s): Q9U3F3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 25, 2002
Last modified: October 29, 2014
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3