P39745 (SUR1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitogen-activated protein kinase mpk-1
EC=2.7.11.24

Alternative name(s):
MAP kinase sur-1

Gene names

Name:	mpk-1
Synonyms:	sur-1
ORF Names:	F43C1.2

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Function in let-60 Ras signaling pathway; acts downstream of lin-45 raf kinase, but before the lin-1 gene product in controlling vulval cell differentiation.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity.
Enzyme regulation	Activated by tyrosine and threonine phosphorylation By similarity.
Domain	The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational modification	Dually phosphorylated on Thr-256 and Tyr-258, which activates the enzyme By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cell cycle
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	Ras protein signal transduction Inferred from genetic interaction. Source: WormBase apoptotic process Inferred from mutant phenotype. Source: WormBase determination of adult lifespan Inferred from mutant phenotype. Source: WormBase embryo development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase germline cell cycle switching, mitotic to meiotic cell cycle Inferred from mutant phenotype. Source: WormBase growth Inferred from mutant phenotype. Source: WormBase hermaphrodite genitalia development Inferred from mutant phenotype. Source: WormBase negative regulation of vulval development Inferred from mutant phenotype. Source: WormBase peptidyl-serine phosphorylation Inferred from direct assay. Source: WormBase vulval development Inferred from mutant phenotype Ref.1Ref.2. Source: UniProtKB
Cellular component	cytoplasm Inferred from direct assay. Source: WormBase nucleus Inferred from direct assay. Source: WormBase
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase activity Inferred from mutant phenotype Ref.2. Source: UniProtKB protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
gla-3	O02289	3	EBI-321013,EBI-317795

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform b (identifier: P39745-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform a (identifier: P39745-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-68: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 444	444	Mitogen-activated protein kinase mpk-1		PRO_0000186306
Regions
	Domain	96 – 384	289	Protein kinase
	Nucleotide binding	102 – 110	9	ATP By similarity
	Motif	256 – 258	3	TXY
Sites
	Active site	220	1	Proton acceptor By similarity
	Binding site	125	1	ATP By similarity
Amino acid modifications
	Modified residue	256	1	Phosphothreonine By similarity
	Modified residue	258	1	Phosphotyrosine By similarity
Natural variations
	Alternative sequence	1 – 68	68	Missing in isoform a.		VSP_004848
Experimental info
	Mutagenesis	106	1	A → V: Loss of function and ATP-binding.
	Sequence conflict	9 – 10	2	LC → FF in AAA18956. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform b [UniParc]. Last modified November 25, 2002. Version 2. Checksum: 11BA27D17641980D	FASTA	444	50,663
	10 20 30 40 50 60 MPTWIPNNLC AQPTTRNAKP PSNGHPQATQ QQSAPGSLAY RNSSNIPNGA TNHVRQQKWQ 70 80 90 100 110 120 YTRSGHRKMA DGEAVISTVN NVEEVHGQLF EVAPRYVNLS YIGEGAYGMV ASALDTITRD 130 140 150 160 170 180 RVAIKKISPF EHQTFCQRTL REIKILNRFK HENIINIQEI IRSETVDSLK DIYIVQCLME 190 200 210 220 230 240 TDLYKLLKTQ KLSNDHVCYF LYQILRGLKY IHSANVLHRD LKPSNLLLNT TCDLKICDFG 250 260 270 280 290 300 LARVTDPQTD HTGFLTEYVA TRWYRAPEIM LNSKGYTKSI DVWSVGCILA EMLSNRPLFP 310 320 330 340 350 360 GKHYLDQLNL ILAVVGSPSN ADLQCIINDK ARSYLISLPH KPKQPWARLY PGADPRALDL 370 380 390 400 410 420 LDKMLTFNPH NRIDIEQALA HPYLEQYYDP GDEPVCEEPF TLEMEFDDLP KEKLKELIWE 430 440 EAEAHHRRME AEAAARNNGG QNPV
	Isoform a [UniParc]. Checksum: 9974B791EFE16838	FASTA	376	43,102
	10 20 30 40 50 60 MADGEAVIST VNNVEEVHGQ LFEVAPRYVN LSYIGEGAYG MVASALDTIT RDRVAIKKIS 70 80 90 100 110 120 PFEHQTFCQR TLREIKILNR FKHENIINIQ EIIRSETVDS LKDIYIVQCL METDLYKLLK 130 140 150 160 170 180 TQKLSNDHVC YFLYQILRGL KYIHSANVLH RDLKPSNLLL NTTCDLKICD FGLARVTDPQ 190 200 210 220 230 240 TDHTGFLTEY VATRWYRAPE IMLNSKGYTK SIDVWSVGCI LAEMLSNRPL FPGKHYLDQL 250 260 270 280 290 300 NLILAVVGSP SNADLQCIIN DKARSYLISL PHKPKQPWAR LYPGADPRAL DLLDKMLTFN 310 320 330 340 350 360 PHNRIDIEQA LAHPYLEQYY DPGDEPVCEE PFTLEMEFDD LPKEKLKELI WEEAEAHHRR 370 MEAEAAARNN GGQNPV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Suppression of activated Let-60 ras protein defines a role of Caenorhabditis elegans Sur-1 MAP kinase in vulval differentiation." Wu Y., Han M. Genes Dev. 8:147-159(1994) [PubMed: 8299935] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B). Strain: Bristol N2.
[2]	"A MAP kinase homolog, mpk-1, is involved in ras-mediated induction of vulval cell fates in Caenorhabditis elegans." Lackner M.R., Kornfeld K., Miller L.M., Horvitz H.R., Kim S.K. Genes Dev. 8:160-173(1994) [PubMed: 8299936] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A). Strain: Bristol N2.
[3]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U03879 mRNA. Translation: AAA18956.1. U27124 mRNA. Translation: AAA73482.1. Z46937 Genomic DNA. Translation: CAA87057.1. Z46937 Genomic DNA. Translation: CAB60996.1.
PIR	A36977. A36978.
RefSeq	NP_001022583.1. NM_001027412.2. NP_001022584.1. NM_001027413.2.
UniGene	Cel.34032.
3D structure databases
ProteinModelPortal	P39745.
SMR	P39745. Positions 90-421.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-26227N.
IntAct	P39745. 42 interactions.
MINT	MINT-114245.
STRING	P39745.
Proteomic databases
PRIDE	P39745.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	F43C1.2b; F43C1.2b; F43C1.2.
GeneID	175545.
KEGG	cel:F43C1.2.
UCSC	F43C1.2a.1. c. elegans.
Organism-specific databases
CTD	175545.
WormBase	F43C1.2a; CE01583; WBGene00003401; mpk-1. F43C1.2b; CE24971; WBGene00003401; mpk-1.
Phylogenomic databases
eggNOG	meNOG04730.
GeneTree	EMGT00050000001140.
HOGENOM	HBG755340.
InParanoid	P39745.
OMA	HAYLSPH.
PhylomeDB	P39745.
Enzyme and pathway databases
BRENDA	2.7.11.24. 1045.
Gene expression databases
ArrayExpress	P39745.
Family and domain databases
InterPro	IPR008349. Erk_1_2_MAPK. IPR011009. Kinase-like_dom. IPR003527. MAP_kinase_CS. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
KO	K04371.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PRINTS	PR01770. ERK1ERK2MAPK.
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS01351. MAPK. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	888610.

Entry information

Entry name

SUR1_CAEEL

Accession

Primary (citable) accession number: P39745
Secondary accession number(s): Q9U3F3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	November 25, 2002
Last modified:	January 25, 2012
This is version 110 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project